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P0AG74

- RUSA_ECOLI

UniProt

P0AG74 - RUSA_ECOLI

Protein

Crossover junction endodeoxyribonuclease RusA

Gene

rusA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of ruvAB or ruvC.

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

    Cofactori

    Binds 1 magnesium ion per subunit.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701MagnesiumCurated
    Metal bindingi72 – 721MagnesiumCurated
    Metal bindingi91 – 911MagnesiumCurated

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: EcoCyc
    2. magnesium ion binding Source: InterPro
    3. recombination hotspot binding Source: EcoCyc

    GO - Biological processi

    1. DNA catabolic process, endonucleolytic Source: GOC
    2. DNA recombination Source: UniProtKB-KW
    3. DNA repair Source: EcoCyc

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G6306-MONOMER.
    ECOL316407:JW0538-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RusA (EC:3.1.22.4)
    Alternative name(s):
    Holliday junction nuclease RusA
    Holliday junction resolvase
    Gene namesi
    Name:rusA
    Synonyms:rus, ybcP
    Ordered Locus Names:b0550, JW0538
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG20283. rusA.

    Subcellular locationi

    GO - Cellular componenti

    1. Holliday junction resolvase complex Source: EcoCyc

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi16 – 161R → Q: No effect on ability to promote DNA repair. 1 Publication
    Mutagenesisi17 – 171Y → L: No effect on ability to promote DNA repair. 1 Publication
    Mutagenesisi19 – 191R → Q: No effect on ability to promote DNA repair. 1 Publication
    Mutagenesisi58 – 581R → Q: No effect on ability to promote DNA repair. 1 Publication
    Mutagenesisi68 – 681R → Q: No effect on ability to promote DNA repair. 1 Publication
    Mutagenesisi69 – 691R → Q or A: Loss of ability to promote DNA repair. Great loss of Holliday junction resolvase activity. No effect on DNA binding. 1 Publication
    Mutagenesisi70 – 701D → N: Reduces junction resolution 80-fold. No effect on DNA binding. 2 Publications
    Mutagenesisi72 – 721D → N: Loss of ability to resolve junctions. No effect on DNA binding. 1 Publication
    Mutagenesisi73 – 731N → A: Slight reduction in ability to promote DNA repair. Great reduction in Holliday junction resolution activity. 1 Publication
    Mutagenesisi76 – 761K → Q: Loss of ability to promote DNA repair. Loss of Holliday junction resolvase activity. No effect on DNA binding. 1 Publication
    Mutagenesisi76 – 761K → R: Loss of ability to promote DNA repair. Less than 2% activity of Holliday junction resolvase. No effect on DNA binding. 1 Publication
    Mutagenesisi80 – 801D → N: Slight reduction in ability to resolve junctions. No effect on DNA binding. 1 Publication
    Mutagenesisi87 – 871F → Y or V: No effect on ability to promote DNA repair. 1 Publication
    Mutagenesisi90 – 901D → N: Slight reduction in ability to resolve junctions. No effect on DNA binding. 1 Publication
    Mutagenesisi91 – 911D → N: Loss of ability to resolve junctions. No effect on DNA binding. 1 Publication
    Mutagenesisi101 – 1011K → Q: No effect on ability to promote DNA repair. 1 Publication
    Mutagenesisi111 – 1111E → Q: No effect on resolvase activity or DNA binding. 1 Publication
    Mutagenesisi116 – 1161E → Q: No effect on resolvase activity or DNA binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 120120Crossover junction endodeoxyribonuclease RusAPRO_0000192004Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP0AG74.

    Interactioni

    Subunit structurei

    Homodimer.3 Publications

    Protein-protein interaction databases

    BioGridi849560. 4 interactions.
    DIPiDIP-16988N.
    IntActiP0AG74. 1 interaction.
    STRINGi511145.b0550.

    Structurei

    Secondary structure

    1
    120
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 97
    Helixi14 – 174
    Beta strandi18 – 236
    Beta strandi25 – 273
    Helixi29 – 4416
    Beta strandi55 – 628
    Beta strandi64 – 674
    Helixi72 – 8413
    Beta strandi87 – 893
    Helixi91 – 933
    Beta strandi94 – 1029
    Beta strandi109 – 1168

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q8RX-ray1.90A/B1-120[»]
    2H8CX-ray3.10A/B/C/D1-120[»]
    2H8EX-ray1.20A1-120[»]
    ProteinModelPortaliP0AG74.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AG74.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni13 – 164DNA binding
    Regioni66 – 738DNA binding

    Sequence similaritiesi

    Belongs to the RusA family.Curated

    Phylogenomic databases

    eggNOGiCOG4570.
    HOGENOMiHOG000119986.
    KOiK01160.
    OMAiRGIHYIS.
    OrthoDBiEOG66HVH9.

    Family and domain databases

    Gene3Di3.30.1330.70. 1 hit.
    InterProiIPR016281. Endonuclease_RusA.
    IPR008822. Endonuclease_RusA-like.
    [Graphical view]
    PfamiPF05866. RusA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001007. RusA. 1 hit.
    SUPFAMiSSF103084. SSF103084. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AG74-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTYSITLPW PPSNNRYYRH NRGRTHVSAE GQAYRDNVAR IIKNAMLDIG    50
    LAMPVKIRIE CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV 100
    KMPVTKGGRL ELTITEMGNE 120
    Length:120
    Mass (Da):13,846
    Last modified:December 20, 2005 - v1
    Checksum:i38401669E8819EA6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92587 Genomic DNA. Translation: CAA63321.1.
    U82598 Genomic DNA. Translation: AAB40746.1.
    U00096 Genomic DNA. Translation: AAC73651.1.
    AP009048 Genomic DNA. Translation: BAE76325.1.
    PIRiS66590.
    RefSeqiNP_415082.1. NC_000913.3.
    YP_488837.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73651; AAC73651; b0550.
    BAE76325; BAE76325; BAE76325.
    GeneIDi12932535.
    945174.
    KEGGiecj:Y75_p0535.
    eco:b0550.
    PATRICi32116262. VBIEscCol129921_0571.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92587 Genomic DNA. Translation: CAA63321.1 .
    U82598 Genomic DNA. Translation: AAB40746.1 .
    U00096 Genomic DNA. Translation: AAC73651.1 .
    AP009048 Genomic DNA. Translation: BAE76325.1 .
    PIRi S66590.
    RefSeqi NP_415082.1. NC_000913.3.
    YP_488837.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Q8R X-ray 1.90 A/B 1-120 [» ]
    2H8C X-ray 3.10 A/B/C/D 1-120 [» ]
    2H8E X-ray 1.20 A 1-120 [» ]
    ProteinModelPortali P0AG74.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 849560. 4 interactions.
    DIPi DIP-16988N.
    IntActi P0AG74. 1 interaction.
    STRINGi 511145.b0550.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73651 ; AAC73651 ; b0550 .
    BAE76325 ; BAE76325 ; BAE76325 .
    GeneIDi 12932535.
    945174.
    KEGGi ecj:Y75_p0535.
    eco:b0550.
    PATRICi 32116262. VBIEscCol129921_0571.

    Organism-specific databases

    EchoBASEi EB4170.
    EcoGenei EG20283. rusA.

    Phylogenomic databases

    eggNOGi COG4570.
    HOGENOMi HOG000119986.
    KOi K01160.
    OMAi RGIHYIS.
    OrthoDBi EOG66HVH9.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6306-MONOMER.
    ECOL316407:JW0538-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AG74.
    PROi P0AG74.

    Gene expression databases

    Genevestigatori P0AG74.

    Family and domain databases

    Gene3Di 3.30.1330.70. 1 hit.
    InterProi IPR016281. Endonuclease_RusA.
    IPR008822. Endonuclease_RusA-like.
    [Graphical view ]
    Pfami PF05866. RusA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001007. RusA. 1 hit.
    SUPFAMi SSF103084. SSF103084. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82."
      Mahdi A.A., Sharples G.J., Mandal T.N., Lloyd R.G.
      J. Mol. Biol. 257:561-573(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions."
      Sharples G.J., Chan S.N., Mahdi A.A., Whitby M.C., Lloyd R.G.
      EMBO J. 13:6133-6142(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-9, CHARACTERIZATION.
    6. "Sequence specificity and biochemical characterization of the RusA Holliday junction resolvase of Escherichia coli."
      Chan S.N., Harris L., Bolt E.L., Whitby M.C., Lloyd R.G.
      J. Biol. Chem. 272:14873-14882(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, SUBUNIT.
    7. "Structural recognition and distortion by the DNA junction-resolving enzyme RusA."
      Giraud-Panis M.-J.E., Lilley D.M.J.
      J. Mol. Biol. 278:117-133(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE SPECIFICITY, DNA-BINDING, MUTAGENESIS OF ASP-70.
    8. "Identification of three aspartic acid residues essential for catalysis by the RusA holliday junction resolvase."
      Bolt E.L., Sharples G.J., Lloyd R.G.
      J. Mol. Biol. 286:403-415(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ASP-70; ASP-72; ASP-80; ASP-90; ASP-91; GLU-111 AND GLU-116.
    9. "Analysis of conserved basic residues associated with DNA binding (Arg69) and catalysis (Lys76) by the RusA holliday junction resolvase."
      Bolt E.L., Sharples G.J., Lloyd R.G.
      J. Mol. Biol. 304:165-176(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ARG-16; TYR-17; ARG-19; ARG-58; ARG-68; ARG-69; ASN-73; LYS-76; PHE-87 AND LYS-101.
    10. "The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold."
      Rafferty J.B., Bolt E.L., Muranova T.A., Sedelnikova S.E., Leonard P., Pasquo A., Baker P.J., Rice D.W., Sharples G.J., Lloyd R.G.
      Structure 11:1557-1567(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT.
    11. "RusA Holliday junction resolvase: DNA complex structure -- insights into selectivity and specificity."
      Macmaster R., Sedelnikova S., Baker P.J., Bolt E.L., Lloyd R.G., Rafferty J.B.
      Nucleic Acids Res. 34:5577-5584(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) OF MUTANT ASN-70 IN COMPLEX WITH DNA, SUBUNIT.

    Entry informationi

    Entry nameiRUSA_ECOLI
    AccessioniPrimary (citable) accession number: P0AG74
    Secondary accession number(s): P40116, Q2MBN1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Encoded by the cryptic lambdoid prophage DLP12. Normally not expressed. Complete suppression of ruv mutations by RusA is dependent on the activity of RecG.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3