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Protein

Crossover junction endodeoxyribonuclease RusA

Gene

rusA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of ruvAB or ruvC.

Miscellaneous

Encoded by the cryptic lambdoid prophage DLP12. Normally not expressed. Complete suppression of ruv mutations by RusA is dependent on the activity of RecG.

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+CuratedNote: Binds 1 Mg2+ ion per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70MagnesiumCurated1
Metal bindingi72MagnesiumCurated1
Metal bindingi91MagnesiumCurated1

GO - Molecular functioni

  • crossover junction endodeoxyribonuclease activity Source: EcoCyc
  • magnesium ion binding Source: InterPro
  • recombination hotspot binding Source: EcoCyc

GO - Biological processi

  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: EcoCyc

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6306-MONOMER
BRENDAi3.1.22.4 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RusA (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase
Gene namesi
Name:rusA
Synonyms:rus, ybcP
Ordered Locus Names:b0550, JW0538
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20283 rusA

Subcellular locationi

GO - Cellular componenti

  • Holliday junction resolvase complex Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi16R → Q: No effect on ability to promote DNA repair. 1 Publication1
Mutagenesisi17Y → L: No effect on ability to promote DNA repair. 1 Publication1
Mutagenesisi19R → Q: No effect on ability to promote DNA repair. 1 Publication1
Mutagenesisi58R → Q: No effect on ability to promote DNA repair. 1 Publication1
Mutagenesisi68R → Q: No effect on ability to promote DNA repair. 1 Publication1
Mutagenesisi69R → Q or A: Loss of ability to promote DNA repair. Great loss of Holliday junction resolvase activity. No effect on DNA binding. 1 Publication1
Mutagenesisi70D → N: Reduces junction resolution 80-fold. No effect on DNA binding. 2 Publications1
Mutagenesisi72D → N: Loss of ability to resolve junctions. No effect on DNA binding. 1 Publication1
Mutagenesisi73N → A: Slight reduction in ability to promote DNA repair. Great reduction in Holliday junction resolution activity. 1 Publication1
Mutagenesisi76K → Q: Loss of ability to promote DNA repair. Loss of Holliday junction resolvase activity. No effect on DNA binding. 1 Publication1
Mutagenesisi76K → R: Loss of ability to promote DNA repair. Less than 2% activity of Holliday junction resolvase. No effect on DNA binding. 1 Publication1
Mutagenesisi80D → N: Slight reduction in ability to resolve junctions. No effect on DNA binding. 1 Publication1
Mutagenesisi87F → Y or V: No effect on ability to promote DNA repair. 1 Publication1
Mutagenesisi90D → N: Slight reduction in ability to resolve junctions. No effect on DNA binding. 1 Publication1
Mutagenesisi91D → N: Loss of ability to resolve junctions. No effect on DNA binding. 1 Publication1
Mutagenesisi101K → Q: No effect on ability to promote DNA repair. 1 Publication1
Mutagenesisi111E → Q: No effect on resolvase activity or DNA binding. 1 Publication1
Mutagenesisi116E → Q: No effect on resolvase activity or DNA binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001920041 – 120Crossover junction endodeoxyribonuclease RusAAdd BLAST120

Proteomic databases

PaxDbiP0AG74
PRIDEiP0AG74

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

BioGridi4261529, 59 interactors
849560, 4 interactors
DIPiDIP-16988N
IntActiP0AG74, 1 interactor
STRINGi316385.ECDH10B_0506

Structurei

Secondary structure

1120
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi14 – 17Combined sources4
Beta strandi18 – 23Combined sources6
Beta strandi25 – 27Combined sources3
Helixi29 – 44Combined sources16
Beta strandi55 – 62Combined sources8
Beta strandi64 – 67Combined sources4
Helixi72 – 84Combined sources13
Beta strandi87 – 89Combined sources3
Helixi91 – 93Combined sources3
Beta strandi94 – 102Combined sources9
Beta strandi109 – 116Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q8RX-ray1.90A/B1-120[»]
2H8CX-ray3.10A/B/C/D1-120[»]
2H8EX-ray1.20A1-120[»]
ProteinModelPortaliP0AG74
SMRiP0AG74
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AG74

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 16DNA binding4
Regioni66 – 73DNA binding8

Sequence similaritiesi

Belongs to the RusA family.Curated

Phylogenomic databases

eggNOGiENOG4106CZA Bacteria
COG4570 LUCA
HOGENOMiHOG000119986
InParanoidiP0AG74
KOiK01160
OMAiIHYISDW

Family and domain databases

Gene3Di3.30.1330.70, 1 hit
InterProiView protein in InterPro
IPR016281 Endonuclease_RusA
IPR008822 Endonuclease_RusA-like
IPR036614 RusA-like_sf
PfamiView protein in Pfam
PF05866 RusA, 1 hit
PIRSFiPIRSF001007 RusA, 1 hit
SUPFAMiSSF103084 SSF103084, 1 hit

Sequencei

Sequence statusi: Complete.

P0AG74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTYSITLPW PPSNNRYYRH NRGRTHVSAE GQAYRDNVAR IIKNAMLDIG
60 70 80 90 100
LAMPVKIRIE CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV
110 120
KMPVTKGGRL ELTITEMGNE
Length:120
Mass (Da):13,846
Last modified:December 20, 2005 - v1
Checksum:i38401669E8819EA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92587 Genomic DNA Translation: CAA63321.1
U82598 Genomic DNA Translation: AAB40746.1
U00096 Genomic DNA Translation: AAC73651.1
AP009048 Genomic DNA Translation: BAE76325.1
PIRiS66590
RefSeqiNP_415082.1, NC_000913.3
WP_001099712.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73651; AAC73651; b0550
BAE76325; BAE76325; BAE76325
GeneIDi945174
KEGGiecj:JW0538
eco:b0550
PATRICifig|1411691.4.peg.1727

Similar proteinsi

Entry informationi

Entry nameiRUSA_ECOLI
AccessioniPrimary (citable) accession number: P0AG74
Secondary accession number(s): P40116, Q2MBN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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