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P0AG74 (RUSA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RusA

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase
Gene names
Name:rusA
Synonyms:rus, ybcP
Ordered Locus Names:b0550, JW0538
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length120 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of ruvAB or ruvC.

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactor

Binds 1 magnesium ion per subunit Probable.

Subunit structure

Homodimer. Ref.6 Ref.10 Ref.11

Miscellaneous

Encoded by the cryptic lambdoid prophage DLP12. Normally not expressed. Complete suppression of ruv mutations by RusA is dependent on the activity of RecG.

Sequence similarities

Belongs to the RusA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 120120Crossover junction endodeoxyribonuclease RusA
PRO_0000192004

Regions

Region13 – 164DNA binding
Region66 – 738DNA binding

Sites

Metal binding701Magnesium Probable
Metal binding721Magnesium Probable
Metal binding911Magnesium Probable

Experimental info

Mutagenesis161R → Q: No effect on ability to promote DNA repair. Ref.9
Mutagenesis171Y → L: No effect on ability to promote DNA repair. Ref.9
Mutagenesis191R → Q: No effect on ability to promote DNA repair. Ref.9
Mutagenesis581R → Q: No effect on ability to promote DNA repair. Ref.9
Mutagenesis681R → Q: No effect on ability to promote DNA repair. Ref.9
Mutagenesis691R → Q or A: Loss of ability to promote DNA repair. Great loss of Holliday junction resolvase activity. No effect on DNA binding. Ref.9
Mutagenesis701D → N: Reduces junction resolution 80-fold. No effect on DNA binding. Ref.7 Ref.8
Mutagenesis721D → N: Loss of ability to resolve junctions. No effect on DNA binding. Ref.8
Mutagenesis731N → A: Slight reduction in ability to promote DNA repair. Great reduction in Holliday junction resolution activity. Ref.9
Mutagenesis761K → Q: Loss of ability to promote DNA repair. Loss of Holliday junction resolvase activity. No effect on DNA binding. Ref.9
Mutagenesis761K → R: Loss of ability to promote DNA repair. Less than 2% activity of Holliday junction resolvase. No effect on DNA binding. Ref.9
Mutagenesis801D → N: Slight reduction in ability to resolve junctions. No effect on DNA binding. Ref.8
Mutagenesis871F → Y or V: No effect on ability to promote DNA repair. Ref.9
Mutagenesis901D → N: Slight reduction in ability to resolve junctions. No effect on DNA binding. Ref.8
Mutagenesis911D → N: Loss of ability to resolve junctions. No effect on DNA binding. Ref.8
Mutagenesis1011K → Q: No effect on ability to promote DNA repair. Ref.9
Mutagenesis1111E → Q: No effect on resolvase activity or DNA binding. Ref.8
Mutagenesis1161E → Q: No effect on resolvase activity or DNA binding. Ref.8

Secondary structure

....................... 120
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AG74 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 38401669E8819EA6

FASTA12013,846
        10         20         30         40         50         60 
MNTYSITLPW PPSNNRYYRH NRGRTHVSAE GQAYRDNVAR IIKNAMLDIG LAMPVKIRIE 

        70         80         90        100        110        120 
CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV KMPVTKGGRL ELTITEMGNE 

« Hide

References

« Hide 'large scale' references
[1]"Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82."
Mahdi A.A., Sharples G.J., Mandal T.N., Lloyd R.G.
J. Mol. Biol. 257:561-573(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions."
Sharples G.J., Chan S.N., Mahdi A.A., Whitby M.C., Lloyd R.G.
EMBO J. 13:6133-6142(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-9, CHARACTERIZATION.
[6]"Sequence specificity and biochemical characterization of the RusA Holliday junction resolvase of Escherichia coli."
Chan S.N., Harris L., Bolt E.L., Whitby M.C., Lloyd R.G.
J. Biol. Chem. 272:14873-14882(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, SUBUNIT.
[7]"Structural recognition and distortion by the DNA junction-resolving enzyme RusA."
Giraud-Panis M.-J.E., Lilley D.M.J.
J. Mol. Biol. 278:117-133(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE SPECIFICITY, DNA-BINDING, MUTAGENESIS OF ASP-70.
[8]"Identification of three aspartic acid residues essential for catalysis by the RusA holliday junction resolvase."
Bolt E.L., Sharples G.J., Lloyd R.G.
J. Mol. Biol. 286:403-415(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ASP-70; ASP-72; ASP-80; ASP-90; ASP-91; GLU-111 AND GLU-116.
[9]"Analysis of conserved basic residues associated with DNA binding (Arg69) and catalysis (Lys76) by the RusA holliday junction resolvase."
Bolt E.L., Sharples G.J., Lloyd R.G.
J. Mol. Biol. 304:165-176(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ARG-16; TYR-17; ARG-19; ARG-58; ARG-68; ARG-69; ASN-73; LYS-76; PHE-87 AND LYS-101.
[10]"The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold."
Rafferty J.B., Bolt E.L., Muranova T.A., Sedelnikova S.E., Leonard P., Pasquo A., Baker P.J., Rice D.W., Sharples G.J., Lloyd R.G.
Structure 11:1557-1567(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT.
[11]"RusA Holliday junction resolvase: DNA complex structure -- insights into selectivity and specificity."
Macmaster R., Sedelnikova S., Baker P.J., Bolt E.L., Lloyd R.G., Rafferty J.B.
Nucleic Acids Res. 34:5577-5584(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) OF MUTANT ASN-70 IN COMPLEX WITH DNA, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X92587 Genomic DNA. Translation: CAA63321.1.
U82598 Genomic DNA. Translation: AAB40746.1.
U00096 Genomic DNA. Translation: AAC73651.1.
AP009048 Genomic DNA. Translation: BAE76325.1.
PIRS66590.
RefSeqNP_415082.1. NC_000913.3.
YP_488837.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q8RX-ray1.90A/B1-120[»]
2H8CX-ray3.10A/B/C/D1-120[»]
2H8EX-ray1.20A1-120[»]
ProteinModelPortalP0AG74.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid849560. 4 interactions.
DIPDIP-16988N.
IntActP0AG74. 1 interaction.
STRING511145.b0550.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73651; AAC73651; b0550.
BAE76325; BAE76325; BAE76325.
GeneID12932535.
945174.
KEGGecj:Y75_p0535.
eco:b0550.
PATRIC32116262. VBIEscCol129921_0571.

Organism-specific databases

EchoBASEEB4170.
EcoGeneEG20283. rusA.

Phylogenomic databases

eggNOGCOG4570.
HOGENOMHOG000119986.
KOK01160.
OMARGIHYIS.
OrthoDBEOG66HVH9.

Enzyme and pathway databases

BioCycEcoCyc:G6306-MONOMER.
ECOL316407:JW0538-MONOMER.

Gene expression databases

GenevestigatorP0AG74.

Family and domain databases

Gene3D3.30.1330.70. 1 hit.
InterProIPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamPF05866. RusA. 1 hit.
[Graphical view]
PIRSFPIRSF001007. RusA. 1 hit.
SUPFAMSSF103084. SSF103084. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0AG74.
PROP0AG74.

Entry information

Entry nameRUSA_ECOLI
AccessionPrimary (citable) accession number: P0AG74
Secondary accession number(s): P40116, Q2MBN1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene