Reviewed, UniProtKB/Swiss-Prot P0AG67 (RS1_ECOLI)

Names and origin

Protein names

Recommended name:
    30S ribosomal protein S1

Gene names

Name:	rpsA
Synonyms:	ssyF
Ordered Locus Names:	b0911, JW0894

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds mRNA; thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence.
Subunit structure	Part of the 30S ribosomal subunit. Some nascent polypeptide chains are able to cross-link to this protein in situ. Can be cross-linked to mRNA in the ribosome.
Post-translational modification	Phosphorylated; probably on a serine. Ref.13
Sequence similarities	Belongs to the ribosomal protein S1P family. Contains 6 S1 motif domains.

Ontologies

Keywords
Domain	Repeat
Ligand	RNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	30S ribosomal protein S1		PRO_0000196033
Regions
	Domain	21 – 87	67	S1 motif 1
	Domain	105 – 171	67	S1 motif 2
	Domain	192 – 260	69	S1 motif 3
	Domain	277 – 347	71	S1 motif 4
	Domain	364 – 434	71	S1 motif 5
	Domain	451 – 520	70	S1 motif 6
Amino acid modifications
	Modified residue	229	1	N6-acetyllysine Ref.14
	Modified residue	279	1	N6-acetyllysine Ref.14
	Modified residue	363	1	N6-acetyllysine Ref.14
Experimental info
	Sequence conflict	125	1	N → D AA sequence Ref.1
	Sequence conflict	181 – 182	2	ER → D in CAA23630. Ref.2
	Sequence conflict	181 – 182	2	ER → D in CAA23644. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0AG67-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 0ABCDEB9E510C267	FASTA	557	61,158
	10 20 30 40 50 60 MTESFAQLFE ESLKEIETRP GSIVRGVVVA IDKDVVLVDA GLKSESAIPA EQFKNAQGEL 70 80 90 100 110 120 EIQVGDEVDV ALDAVEDGFG ETLLSREKAK RHEAWITLEK AYEDAETVTG VINGKVKGGF 130 140 150 160 170 180 TVELNGIRAF LPGSLVDVRP VRDTLHLEGK ELEFKVIKLD QKRNNVVVSR RAVIESENSA 190 200 210 220 230 240 ERDQLLENLQ EGMEVKGIVK NLTDYGAFVD LGGVDGLLHI TDMAWKRVKH PSEIVNVGDE 250 260 270 280 290 300 ITVKVLKFDR ERTRVSLGLK QLGEDPWVAI AKRYPEGTKL TGRVTNLTDY GCFVEIEEGV 310 320 330 340 350 360 EGLVHVSEMD WTNKNIHPSK VVNVGDVVEV MVLDIDEERR RISLGLKQCK ANPWQQFAET 370 380 390 400 410 420 HNKGDRVEGK IKSITDFGIF IGLDGGIDGL VHLSDISWNV AGEEAVREYK KGDEIAAVVL 430 440 450 460 470 480 QVDAERERIS LGVKQLAEDP FNNWVALNKK GAIVTGKVTA VDAKGATVEL ADGVEGYLRA 490 500 510 520 530 540 SEASRDRVED ATLVLSVGDE VEAKFTGVDR KNRAISLSVR AKDEADEKDA IATVNKQEDA 550 NFSNNAMAEA FKAAKGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA." Schnier J., Kimura M., Foulaki K., Subramanian A.R., Isono K., Wittmann-Liebold B. Proc. Natl. Acad. Sci. U.S.A. 79:1008-1011(1982) [PubMed: 7041110] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: MRE-600.
[2]	"The DNA sequence of the gene rpsA of Escherichia coli coding for ribosomal protein S1." Schnier J., Isono K. Nucleic Acids Res. 10:1857-1865(1982) [PubMed: 6281725] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / JS6.5.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Transcriptional organization of the rpsA operon of Escherichia coli." Pedersen S., Skouv J., Kajitani M., Ishihama A. Mol. Gen. Genet. 196:135-140(1984) [PubMed: 6384724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
[7]	Bienvenut W.V., Barblan J., Quadroni M. Submitted (JAN-2004) to UniProtKB Cited for: PROTEIN SEQUENCE OF 118-128, MASS SPECTROMETRY.
[8]	"Primary structure of the hip gene of Escherichia coli and of its product, the beta subunit of integration host factor." Flamm E., Weisberg R.A. J. Mol. Biol. 183:117-128(1985) [PubMed: 3159903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 550-557. Strain: K12.
[9]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-11. Strain: K12 / EMG2.
[10]	Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F. Submitted (FEB-1996) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-11. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[11]	"The path of mRNA through the Escherichia coli ribosome; site-directed cross-linking of mRNA analogues carrying a photo-reactive label at various points 3' to the decoding site." Rinke-Appel J., Juenke N., Stade K., Brimacombe R. EMBO J. 10:2195-2202(1991) [PubMed: 1712292] [Abstract] Cited for: CROSS-LINKING TO MRNA.
[12]	"Flexibility of the nascent polypeptide chain within the ribosome --contacts from the peptide N-terminus to a specific region of the 30S subunit." Choi K.M., Atkins J.F., Gesteland R.F., Brimacombe R. Eur. J. Biochem. 255:409-413(1998) [PubMed: 9716382] [Abstract] Cited for: CROSS-LINKING TO NASCENT POLYPEPTIDE CHAINS.
[13]	"Identification of phosphoproteins in Escherichia coli." Freestone P., Grant S., Toth I., Norris V. Mol. Microbiol. 15:573-580(1995) [PubMed: 7783627] [Abstract] Cited for: PHOSPHORYLATION, PROTEIN SEQUENCE OF 1-13.
[14]	"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y. Mol. Cell. Proteomics 8:215-225(2009) [PubMed: 18723842] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-229; LYS-279 AND LYS-363, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

V00342 Genomic DNA. Translation: CAA23630.1.
V00352 Genomic DNA. Translation: CAA23644.1.
U00096 Genomic DNA. Translation: AAC73997.1.
AP009048 Genomic DNA. Translation: BAA35655.1.
X00785 Genomic DNA. Translation: CAA25361.1.
X04864 Genomic DNA. Translation: CAA28556.1.

PIR

R3EC1. F64830.

RefSeq

AP_001541.1.
NP_415431.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BH8	X-ray	1.90	A/B	364-398	[»]

ModBase

Search...

PTM databases

PhosSite

P0AG67.

2-D gel databases

SWISS-2DPAGE

P0AG67.

2DBase-Ecoli

P0AG67.

ECO2DBASE

B065.0. 6TH EDITION.

Genome annotation databases

GeneID

945536.

GenomeReviews

Gene locus JW0894 in contig AP009048_GR.
Gene locus b0911 in contig U00096_GR.

KEGG

ecj:JW0894.
eco:b0911.

Organism-specific databases

EchoBASE

EB0893.

EcoGene

EG10900. rpsA.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AG67.

OMA

P0AG67. SITDYGA.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10900-MON.

Family and domain databases

InterPro

IPR012340. NA-bd_OB-fold.
IPR018032. Rbsml-S1_synth_mod.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR000110. Ribosomal_S1.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 4 hits.

Pfam

PF00575. S1. 6 hits.
[Graphical view]

PRINTS

PR00681. RIBOSOMALS1.

TIGRFAMs

TIGR00717. rpsA. 1 hit.

PROSITE

PS50126. S1. 6 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RS1_ECOLI

Accession

Primary (citable) accession number: P0AG67
Secondary accession number(s): P02349, P77352

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)