30S ribosomal protein S17 - Escherichia coli (strain K12)

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Names and origin

Protein names

Recommended name:
30S ribosomal protein S17

Gene names

Name:	rpsQ
Synonyms:	neaA
Ordered Locus Names:	b3311, JW3273

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	84 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. Also plays a role in translational accuracy; neamine-resistant ribosomes show reduced neamine-induced misreading in vitro. HAMAP MF_01345
Subunit structure	Part of the 30S ribosomal subunit. Ref.8
Miscellaneous	The strain missing both S17 and L29 grows very slowly and has a rather unstable temperature-sensitive phenotype. HAMAP MF_01345
Sequence similarities	Belongs to the ribosomal protein S17P family.
Mass spectrometry	Molecular mass is 9573.0 Da from positions 2 - 84. Determined by MALDI. Ref.11

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Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.4 Ref.6

Chain

2 – 84

30S ribosomal protein S17 HAMAP MF_01345

PRO_0000128456

Natural variations

Natural variant

H → P in neamine-resistant mutant nea301. HAMAP MF_01345

Natural variant

S → F Prevents 30S subunit assembly at 42 degrees Celsius. Ref.9

Experimental info

Sequence conflict

53 – 59

CGIGDVV → GIGDVVC AA sequence Ref.4

Sequence conflict

64 – 66

CRP → RPC Ref.5

Secondary structure

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0AG63-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: FC64AF04841842F6
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FASTA

9,704

        10         20         30         40         50         60 
MTDKIRTLQG RVVSDKMEKS IVVAIERFVK HPIYGKFIKR TTKLHVHDEN NECGIGDVVE 

        70         80 
IRECRPLSKT KSWTLVRVVE KAVL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the Escherichia coli S10 ribosomal protein operon." Zurawski G., Zurawski S.M. Nucleic Acids Res. 13:4521-4526(1985) [PubMed: 3892488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The primary structure of protein S17 from the small ribosomal subunit of Escherichia coli." Yaguchi M., Wittmann H.G. FEBS Lett. 87:37-40(1978) [PubMed: 344065] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-84. Strain: K.
[5]	"DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coli." Post L.E., Arfsten A.E., Reusser F., Nomura M. Cell 15:215-229(1978) [PubMed: 151587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-84. Strain: K12.
[6]	"Small genes/gene-products in Escherichia coli K-12." Wasinger V.C., Humphery-Smith I. FEMS Microbiol. Lett. 169:375-382(1998) [PubMed: 9868784] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11. Strain: K12.
[7]	"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies." Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B. EMBO J. 14:4578-4588(1995) [PubMed: 7556101] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-36, CROSS-LINKING TO RRNA. Strain: MRE-600.
[8]	"Assembly mapping of 30 S ribosomal proteins from Escherichia coli. Further studies." Held W.A., Ballou B., Mizushima S., Nomura M. J. Biol. Chem. 249:3103-3111(1974) [PubMed: 4598121] [Abstract] Cited for: IDENTIFICATION AS A PRIMARY 16S RRNA BINDING PROTEIN, ASSEMBLY MAP OF THE 30S SUBUNIT. Strain: Q13.
[9]	"A missense mutation in the gene coding for ribosomal protein S17 (rpsQ) leading to ribosomal assembly defectivity in Escherichia coli." Herzog A., Yaguchi M., Cabezon T., Corchuelo M.C., Petre J., Bollen A. Mol. Gen. Genet. 171:15-22(1979) [PubMed: 108517] [Abstract] Cited for: THERMOSENSITIVE VARIANT PHE-68 AFFECTS ASSEMBLY.
[10]	"A mutant from Escherichia coli which lacks ribosomal proteins S17 and L29 used to localize these two proteins on the ribosomal surface." Stoeffler-Meilicke M., Dabbs E.R., Albrecht-Ehrlich R., Stoeffler G. Eur. J. Biochem. 150:485-490(1985) [PubMed: 3926498] [Abstract] Cited for: CHARACTERIZATION OF A STRAIN MISSING S17 AND L29. Strain: AM111.
[11]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[12]	"Alteration of ribosomal protein S17 by mutation linked to neamine resistance in Escherichia coli. I. General properties of neaA mutants." Bollen A., Cabezon T., de Wilde M., Villarroel R., Herzog A. J. Mol. Biol. 99:795-806(1975) [PubMed: 765484] [Abstract] Cited for: ISOLATION, CHARACTERIZATION OF NEAMINE-RESISTANT VARIANT NEA301. Strain: K12S.
[13]	"Alteration of ribosomal protein S17 by mutation linked to neamine resistance in Escherichia coli. II. Localization of the amino acid replacement in protein S17 from a meaA mutant." Yaguchi M., Wittmann H.G., Cabezon T., DeWilde M., Villarroel R., Herzog A., Bollen A. J. Mol. Biol. 104:617-620(1976) [PubMed: 781296] [Abstract] Cited for: NEAMINE-RESISTANT VARIANT NEA301. Strain: K12S.
[14]	"All-atom homology model of the Escherichia coli 30S ribosomal subunit." Tung C.-S., Joseph S., Sanbonmatsu K.Y. Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[15]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[16]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X02613 Genomic DNA. Translation: CAA26469.1.
U18997 Genomic DNA. Translation: AAA58108.1.
U00096 Genomic DNA. Translation: AAC76336.1.
AP009048 Genomic DNA. Translation: BAE77980.1.
V00357 Genomic DNA. Translation: CAA23652.1.
X01563 Genomic DNA. Translation: CAA25714.1.

PIR

R3EC17. A37519.

RefSeq

AP_004479.1.
NP_417770.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M5G	model	-	Q	4-84	[»]
1P6G	electron microscopy	12.30	Q	1-84	[»]
1P87	electron microscopy	11.50	Q	1-84	[»]
1VS5	X-ray	3.46	Q	1-84	[»]
1VS7	X-ray	3.46	Q	1-84	[»]
2AVY	X-ray	3.46	Q	1-84	[»]
2AW7	X-ray	3.46	Q	1-84	[»]
2GY9	electron microscopy	-	Q	6-83	[»]
2GYB	electron microscopy	-	Q	6-83	[»]
2I2P	X-ray	3.22	Q	2-83	[»]
2I2U	X-ray	3.22	Q	2-83	[»]
2QAL	X-ray	3.21	Q	2-84	[»]
2QAN	X-ray	3.21	Q	2-84	[»]
2QB9	X-ray	3.54	Q	2-84	[»]
2QBB	X-ray	3.54	Q	2-84	[»]
2QBD	X-ray	3.30	Q	2-84	[»]
2QBF	X-ray	3.30	Q	2-84	[»]
2QBH	X-ray	4.00	Q	2-84	[»]
2QBJ	X-ray	4.00	Q	2-84	[»]
2QOU	X-ray	3.93	Q	2-84	[»]
2QOW	X-ray	3.93	Q	2-84	[»]
2QOY	X-ray	3.50	Q	2-84	[»]
2QP0	X-ray	3.50	Q	2-84	[»]
2Z4K	X-ray	4.45	Q	2-84	[»]
2Z4M	X-ray	4.45	Q	2-84	[»]
3FIH	electron microscopy	-	Q	4-83	[»]

ModBase

Search...

2-D gel databases

ECO2DBASE

I012.6. 6TH EDITION.

Genome annotation databases

GeneID

947808.

GenomeReviews

Gene locus JW3273 in contig AP009048_GR.
Gene locus b3311 in contig U00096_GR.

KEGG

ecj:JW3273.
eco:b3311.

Organism-specific databases

EchoBASE

EB0909.

EcoGene

EG10916. rpsQ.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AG63.

OMA

P0AG63. FKAHDEE.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10916-MON.

Family and domain databases

HAMAP

MF_01345.
[Tree]

InterPro

IPR012340. NA-bd_OB-fold.
IPR000266. Ribosomal_S17.
IPR019984. Ribosomal_S17_bac-type.
IPR019979. Ribosomal_S17_CS.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.

PANTHER

PTHR10744. Ribosomal_S17. 1 hit.

Pfam

PF00366. Ribosomal_S17. 1 hit.
[Graphical view]

PRINTS

PR00973. RIBOSOMALS17.

ProDom

PD001295. Ribosomal_S17. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR03635. S17_bact. 1 hit.

PROSITE

PS00056. RIBOSOMAL_S17. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

RS17_ECOLI

Accession

Primary (citable) accession number: P0AG63
Secondary accession number(s): P02373, Q2M6X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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