Reviewed, UniProtKB/Swiss-Prot P0AG59 (RS14_ECOLI)

Names and origin

Protein names

Recommended name:
    30S ribosomal protein S14

Gene names

Name:	rpsN
Ordered Locus Names:	b3307, JW3269

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site. HAMAP MF_00537
Subunit structure	Part of the 30S ribosomal subunit. Contacts proteins S3 and S10 By similarity.
Sequence similarities	Belongs to the ribosomal protein S14P family.
Mass spectrometry	Molecular mass is 11449.3 Da from positions 2 - 101. Determined by MALDI. Ref.5

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 101

100

30S ribosomal protein S14 HAMAP MF_00537

PRO_0000130890

Experimental info

Sequence conflict

92

1

E → Q AA sequence Ref.1

Sequence conflict

99 – 101

3

ASW → G in CAA25718. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

101

Helix Strand Turn

Details...

Turn

3 – 5

3

Helix

6 – 19

14

Turn

20 – 24

5

Helix

25 – 31

7

Helix

43 – 48

6

Beta strand

65 – 67

3

Turn

75 – 77

3

Beta strand

78 – 80

3

Helix

82 – 89

8

Beta strand

93 – 95

3

Sequences

	Sequence		Length	Mass (Da)
	P0AG59-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E2B21D9D3752352C	FASTA	101	11,580
	10 20 30 40 50 60 MAKQSMKARE VKRVALADKY FAKRAELKAI ISDVNASDED RWNAVLKLQT LPRDSSPSRQ 70 80 90 100 RNRCRQTGRP HGFLRKFGLS RIKVREAAMR GEIPGLKKAS W

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Yaguchi M., Roy C., Reithmeier R.A.F., Wittmann-Liebold B. Submitted (OCT-1982) to the PIR data bank Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-101. Strain: K12.
[2]	"The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene." Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M. Nucleic Acids Res. 11:2599-2616(1983) [PubMed: 6222285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[6]	"All-atom homology model of the Escherichia coli 30S ribosomal subunit." Tung C.-S., Joseph S., Sanbonmatsu K.Y. Nat. Struct. Biol. 9:750-755(2002) [PubMed: 12244297] [Abstract] Cited for: 3D-STRUCTURE MODELING.
[7]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[8]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X01563 Genomic DNA. Translation: CAA25718.1.
U18997 Genomic DNA. Translation: AAA58104.1.
U00096 Genomic DNA. Translation: AAC76332.1.
AP009048 Genomic DNA. Translation: BAE77984.1.

PIR

R3EC14. F65123.

RefSeq

AP_004483.1.
NP_417766.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M5G	model	-	N	42-101	[»]
1P6G	electron microscopy	12.30	N	1-101	[»]
1P87	electron microscopy	11.50	N	1-101	[»]
1VS5	X-ray	3.46	N	1-101	[»]
1VS7	X-ray	3.46	N	1-101	[»]
2AVY	X-ray	3.46	N	1-101	[»]
2AW7	X-ray	3.46	N	1-101	[»]
2GY9	electron microscopy	-	N	41-100	[»]
2GYB	electron microscopy	-	N	41-100	[»]
2I2P	X-ray	3.22	N	2-100	[»]
2I2U	X-ray	3.22	N	2-100	[»]
2QAL	X-ray	3.21	N	2-101	[»]
2QAN	X-ray	3.21	N	2-101	[»]
2QB9	X-ray	3.54	N	2-101	[»]
2QBB	X-ray	3.54	N	2-101	[»]
2QBD	X-ray	3.30	N	2-101	[»]
2QBF	X-ray	3.30	N	2-101	[»]
2QBH	X-ray	4.00	N	2-101	[»]
2QBJ	X-ray	4.00	N	2-101	[»]
2QOU	X-ray	3.93	N	2-101	[»]
2QOW	X-ray	3.93	N	2-101	[»]
2QOY	X-ray	3.50	N	2-101	[»]
2QP0	X-ray	3.50	N	2-101	[»]
2VHO	X-ray	3.74	N	2-101	[»]
2VHP	X-ray	3.74	N	2-101	[»]
2Z4K	X-ray	4.45	N	2-101	[»]
2Z4M	X-ray	4.45	N	2-101	[»]
3DF1	X-ray	3.50	N	2-101	[»]
3DF3	X-ray	3.50	N	2-101	[»]
3FIH	electron microscopy	-	N	2-101	[»]

ModBase

Search...

Genome annotation databases

GeneID

947801.

GenomeReviews

Gene locus JW3269 in contig AP009048_GR.
Gene locus b3307 in contig U00096_GR.

KEGG

ecj:JW3269.
eco:b3307.

Organism-specific databases

EchoBASE

EB0906.

EcoGene

EG10913. rpsN.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AG59.

OMA

P0AG59. RLEIHRK.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10913-MON.

Family and domain databases

HAMAP

MF_00537.
[Tree]

InterPro

IPR001209. Ribosomal_S14.
IPR018271. Ribosomal_S14_CS.
[Graphical view]

PANTHER

PTHR19836. Ribosomal_S14. 1 hit.

Pfam

PF00253. Ribosomal_S14. 1 hit.
[Graphical view]

PROSITE

PS00527. RIBOSOMAL_S14. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RS14_ECOLI

Accession

Primary (citable) accession number: P0AG59
Secondary accession number(s): P02370, Q2M6X2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)