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P0AG59

- RS14_ECOLI

UniProt

P0AG59 - RS14_ECOLI

Protein

30S ribosomal protein S14

Gene

rpsN

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-HAMAP
    2. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10913-MONOMER.
    ECOL316407:JW3269-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    30S ribosomal protein S14
    Gene namesi
    Name:rpsN
    Ordered Locus Names:b3307, JW3269
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10913. rpsN.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 10110030S ribosomal protein S14PRO_0000130890Add
    BLAST

    Proteomic databases

    PaxDbiP0AG59.
    PRIDEiP0AG59.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AG59.

    Interactioni

    Subunit structurei

    Part of the 30S ribosomal subunit. Contacts proteins S3 and S10 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-35805N.
    IntActiP0AG59. 70 interactions.
    MINTiMINT-1295909.
    STRINGi511145.b3307.

    Structurei

    Secondary structure

    1
    101
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1916
    Turni21 – 233
    Helixi24 – 263
    Turni27 – 315
    Beta strandi32 – 343
    Helixi43 – 475
    Turni49 – 524
    Helixi53 – 553
    Helixi57 – 593
    Turni65 – 673
    Beta strandi71 – 744
    Turni75 – 784
    Helixi81 – 888
    Turni89 – 913
    Beta strandi93 – 953
    Beta strandi96 – 983

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1M5Gmodel-N42-101[»]
    1P6Gelectron microscopy12.30N2-101[»]
    1P87electron microscopy11.50N2-101[»]
    1VS5X-ray3.46N1-101[»]
    1VS7X-ray3.46N1-101[»]
    2AVYX-ray3.46N2-101[»]
    2AW7X-ray3.46N2-101[»]
    2GY9electron microscopy15.00N41-101[»]
    2GYBelectron microscopy15.00N41-101[»]
    2I2PX-ray3.22N2-101[»]
    2I2UX-ray3.22N2-101[»]
    2QALX-ray3.21N2-101[»]
    2QANX-ray3.21N2-101[»]
    2QB9X-ray3.54N2-101[»]
    2QBBX-ray3.54N2-101[»]
    2QBDX-ray3.30N2-101[»]
    2QBFX-ray3.30N2-101[»]
    2QBHX-ray4.00N2-101[»]
    2QBJX-ray4.00N2-101[»]
    2QOUX-ray3.93N2-101[»]
    2QOWX-ray3.93N2-101[»]
    2QOYX-ray3.50N2-101[»]
    2QP0X-ray3.50N2-101[»]
    2VHOX-ray3.74N2-101[»]
    2VHPX-ray3.74N2-101[»]
    2WWLelectron microscopy5.80N2-101[»]
    2YKRelectron microscopy9.80N2-101[»]
    2Z4KX-ray4.45N2-101[»]
    2Z4MX-ray4.45N2-101[»]
    3DF1X-ray3.50N2-101[»]
    3DF3X-ray3.50N2-101[»]
    3E1Aelectron microscopy-G1-101[»]
    3E1Celectron microscopy-G1-101[»]
    3FIHelectron microscopy6.70N2-101[»]
    3I1MX-ray3.19N1-101[»]
    3I1OX-ray3.19N1-101[»]
    3I1QX-ray3.81N1-101[»]
    3I1SX-ray3.81N1-101[»]
    3I1ZX-ray3.71N1-101[»]
    3I21X-ray3.71N1-101[»]
    3IZVelectron microscopy-R1-101[»]
    3IZWelectron microscopy-R1-101[»]
    3J00electron microscopy-N2-101[»]
    3J0Uelectron microscopy12.10Q2-101[»]
    3J0Velectron microscopy14.70Q2-101[»]
    3J0Xelectron microscopy13.50Q2-101[»]
    3J0Zelectron microscopy11.50Q2-101[»]
    3J10electron microscopy11.50Q2-101[»]
    3J13electron microscopy13.10P2-101[»]
    3J18electron microscopy8.30N2-101[»]
    3J36electron microscopy9.80N2-101[»]
    3J4Velectron microscopy12.00N1-101[»]
    3J4Welectron microscopy12.00N1-101[»]
    3J4Yelectron microscopy17.00N1-101[»]
    3J4Zelectron microscopy20.00N1-101[»]
    3J53electron microscopy13.00N1-101[»]
    3J55electron microscopy15.00N1-101[»]
    3J57electron microscopy17.00N1-101[»]
    3J59electron microscopy12.00N1-101[»]
    3J5Belectron microscopy17.00N1-101[»]
    3J5Delectron microscopy17.00N1-101[»]
    3J5Felectron microscopy20.00N1-101[»]
    3J5Helectron microscopy15.00N1-101[»]
    3J5Jelectron microscopy9.00N1-101[»]
    3J5Nelectron microscopy6.80N1-101[»]
    3J5Telectron microscopy7.60N2-101[»]
    3J5Xelectron microscopy7.60N2-101[»]
    3KC4electron microscopy-N1-101[»]
    3OAQX-ray3.25N2-101[»]
    3OARX-ray3.25N2-101[»]
    3OFAX-ray3.19N2-101[»]
    3OFBX-ray3.19N2-101[»]
    3OFOX-ray3.10N2-101[»]
    3OFPX-ray3.10N2-101[»]
    3OFXX-ray3.29N2-101[»]
    3OFYX-ray3.29N2-101[»]
    3OR9X-ray3.30N1-101[»]
    3ORAX-ray3.30N1-101[»]
    3SFSX-ray3.20N1-101[»]
    3UOQX-ray3.70N1-101[»]
    4A2Ielectron microscopy16.50N2-101[»]
    4ADVelectron microscopy13.50N2-101[»]
    4GAQX-ray3.30N1-101[»]
    4GASX-ray3.30N1-101[»]
    4GD1X-ray3.00N2-101[»]
    4GD2X-ray3.00N2-101[»]
    4KIYX-ray2.90N1-101[»]
    4KJ0X-ray2.90N1-101[»]
    4KJ2X-ray2.90N1-101[»]
    4KJ4X-ray2.90N1-101[»]
    4KJ6X-ray2.90N1-101[»]
    4KJ8X-ray2.90N1-101[»]
    4KJAX-ray2.90N1-101[»]
    4KJCX-ray2.90N1-101[»]
    ProteinModelPortaliP0AG59.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AG59.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S14P family.Curated

    Phylogenomic databases

    eggNOGiCOG0199.
    HOGENOMiHOG000039864.
    KOiK02954.
    OMAiRIRNRCW.
    OrthoDBiEOG6BCT0K.
    PhylomeDBiP0AG59.

    Family and domain databases

    HAMAPiMF_00537. Ribosomal_S14_1.
    InterProiIPR001209. Ribosomal_S14.
    IPR023036. Ribosomal_S14_bac/plaastid.
    IPR018271. Ribosomal_S14_CS.
    [Graphical view]
    PANTHERiPTHR19836. PTHR19836. 1 hit.
    PfamiPF00253. Ribosomal_S14. 1 hit.
    [Graphical view]
    PROSITEiPS00527. RIBOSOMAL_S14. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AG59-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKQSMKARE VKRVALADKY FAKRAELKAI ISDVNASDED RWNAVLKLQT    50
    LPRDSSPSRQ RNRCRQTGRP HGFLRKFGLS RIKVREAAMR GEIPGLKKAS 100
    W 101
    Length:101
    Mass (Da):11,580
    Last modified:January 23, 2007 - v2
    Checksum:iE2B21D9D3752352C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti92 – 921E → Q AA sequence 1 PublicationCurated
    Sequence conflicti99 – 1013ASW → G in CAA25718. (PubMed:6222285)Curated

    Mass spectrometryi

    Molecular mass is 11449.3 Da from positions 2 - 101. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01563 Genomic DNA. Translation: CAA25718.1.
    U18997 Genomic DNA. Translation: AAA58104.1.
    U00096 Genomic DNA. Translation: AAC76332.1.
    AP009048 Genomic DNA. Translation: BAE77984.1.
    PIRiF65123. R3EC14.
    RefSeqiNP_417766.1. NC_000913.3.
    YP_492125.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76332; AAC76332; b3307.
    BAE77984; BAE77984; BAE77984.
    GeneIDi12934450.
    947801.
    KEGGiecj:Y75_p3869.
    eco:b3307.
    PATRICi32122046. VBIEscCol129921_3400.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01563 Genomic DNA. Translation: CAA25718.1 .
    U18997 Genomic DNA. Translation: AAA58104.1 .
    U00096 Genomic DNA. Translation: AAC76332.1 .
    AP009048 Genomic DNA. Translation: BAE77984.1 .
    PIRi F65123. R3EC14.
    RefSeqi NP_417766.1. NC_000913.3.
    YP_492125.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1M5G model - N 42-101 [» ]
    1P6G electron microscopy 12.30 N 2-101 [» ]
    1P87 electron microscopy 11.50 N 2-101 [» ]
    1VS5 X-ray 3.46 N 1-101 [» ]
    1VS7 X-ray 3.46 N 1-101 [» ]
    2AVY X-ray 3.46 N 2-101 [» ]
    2AW7 X-ray 3.46 N 2-101 [» ]
    2GY9 electron microscopy 15.00 N 41-101 [» ]
    2GYB electron microscopy 15.00 N 41-101 [» ]
    2I2P X-ray 3.22 N 2-101 [» ]
    2I2U X-ray 3.22 N 2-101 [» ]
    2QAL X-ray 3.21 N 2-101 [» ]
    2QAN X-ray 3.21 N 2-101 [» ]
    2QB9 X-ray 3.54 N 2-101 [» ]
    2QBB X-ray 3.54 N 2-101 [» ]
    2QBD X-ray 3.30 N 2-101 [» ]
    2QBF X-ray 3.30 N 2-101 [» ]
    2QBH X-ray 4.00 N 2-101 [» ]
    2QBJ X-ray 4.00 N 2-101 [» ]
    2QOU X-ray 3.93 N 2-101 [» ]
    2QOW X-ray 3.93 N 2-101 [» ]
    2QOY X-ray 3.50 N 2-101 [» ]
    2QP0 X-ray 3.50 N 2-101 [» ]
    2VHO X-ray 3.74 N 2-101 [» ]
    2VHP X-ray 3.74 N 2-101 [» ]
    2WWL electron microscopy 5.80 N 2-101 [» ]
    2YKR electron microscopy 9.80 N 2-101 [» ]
    2Z4K X-ray 4.45 N 2-101 [» ]
    2Z4M X-ray 4.45 N 2-101 [» ]
    3DF1 X-ray 3.50 N 2-101 [» ]
    3DF3 X-ray 3.50 N 2-101 [» ]
    3E1A electron microscopy - G 1-101 [» ]
    3E1C electron microscopy - G 1-101 [» ]
    3FIH electron microscopy 6.70 N 2-101 [» ]
    3I1M X-ray 3.19 N 1-101 [» ]
    3I1O X-ray 3.19 N 1-101 [» ]
    3I1Q X-ray 3.81 N 1-101 [» ]
    3I1S X-ray 3.81 N 1-101 [» ]
    3I1Z X-ray 3.71 N 1-101 [» ]
    3I21 X-ray 3.71 N 1-101 [» ]
    3IZV electron microscopy - R 1-101 [» ]
    3IZW electron microscopy - R 1-101 [» ]
    3J00 electron microscopy - N 2-101 [» ]
    3J0U electron microscopy 12.10 Q 2-101 [» ]
    3J0V electron microscopy 14.70 Q 2-101 [» ]
    3J0X electron microscopy 13.50 Q 2-101 [» ]
    3J0Z electron microscopy 11.50 Q 2-101 [» ]
    3J10 electron microscopy 11.50 Q 2-101 [» ]
    3J13 electron microscopy 13.10 P 2-101 [» ]
    3J18 electron microscopy 8.30 N 2-101 [» ]
    3J36 electron microscopy 9.80 N 2-101 [» ]
    3J4V electron microscopy 12.00 N 1-101 [» ]
    3J4W electron microscopy 12.00 N 1-101 [» ]
    3J4Y electron microscopy 17.00 N 1-101 [» ]
    3J4Z electron microscopy 20.00 N 1-101 [» ]
    3J53 electron microscopy 13.00 N 1-101 [» ]
    3J55 electron microscopy 15.00 N 1-101 [» ]
    3J57 electron microscopy 17.00 N 1-101 [» ]
    3J59 electron microscopy 12.00 N 1-101 [» ]
    3J5B electron microscopy 17.00 N 1-101 [» ]
    3J5D electron microscopy 17.00 N 1-101 [» ]
    3J5F electron microscopy 20.00 N 1-101 [» ]
    3J5H electron microscopy 15.00 N 1-101 [» ]
    3J5J electron microscopy 9.00 N 1-101 [» ]
    3J5N electron microscopy 6.80 N 1-101 [» ]
    3J5T electron microscopy 7.60 N 2-101 [» ]
    3J5X electron microscopy 7.60 N 2-101 [» ]
    3KC4 electron microscopy - N 1-101 [» ]
    3OAQ X-ray 3.25 N 2-101 [» ]
    3OAR X-ray 3.25 N 2-101 [» ]
    3OFA X-ray 3.19 N 2-101 [» ]
    3OFB X-ray 3.19 N 2-101 [» ]
    3OFO X-ray 3.10 N 2-101 [» ]
    3OFP X-ray 3.10 N 2-101 [» ]
    3OFX X-ray 3.29 N 2-101 [» ]
    3OFY X-ray 3.29 N 2-101 [» ]
    3OR9 X-ray 3.30 N 1-101 [» ]
    3ORA X-ray 3.30 N 1-101 [» ]
    3SFS X-ray 3.20 N 1-101 [» ]
    3UOQ X-ray 3.70 N 1-101 [» ]
    4A2I electron microscopy 16.50 N 2-101 [» ]
    4ADV electron microscopy 13.50 N 2-101 [» ]
    4GAQ X-ray 3.30 N 1-101 [» ]
    4GAS X-ray 3.30 N 1-101 [» ]
    4GD1 X-ray 3.00 N 2-101 [» ]
    4GD2 X-ray 3.00 N 2-101 [» ]
    4KIY X-ray 2.90 N 1-101 [» ]
    4KJ0 X-ray 2.90 N 1-101 [» ]
    4KJ2 X-ray 2.90 N 1-101 [» ]
    4KJ4 X-ray 2.90 N 1-101 [» ]
    4KJ6 X-ray 2.90 N 1-101 [» ]
    4KJ8 X-ray 2.90 N 1-101 [» ]
    4KJA X-ray 2.90 N 1-101 [» ]
    4KJC X-ray 2.90 N 1-101 [» ]
    ProteinModelPortali P0AG59.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35805N.
    IntActi P0AG59. 70 interactions.
    MINTi MINT-1295909.
    STRINGi 511145.b3307.

    Chemistry

    ChEMBLi CHEMBL2363135.

    Proteomic databases

    PaxDbi P0AG59.
    PRIDEi P0AG59.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76332 ; AAC76332 ; b3307 .
    BAE77984 ; BAE77984 ; BAE77984 .
    GeneIDi 12934450.
    947801.
    KEGGi ecj:Y75_p3869.
    eco:b3307.
    PATRICi 32122046. VBIEscCol129921_3400.

    Organism-specific databases

    EchoBASEi EB0906.
    EcoGenei EG10913. rpsN.

    Phylogenomic databases

    eggNOGi COG0199.
    HOGENOMi HOG000039864.
    KOi K02954.
    OMAi RIRNRCW.
    OrthoDBi EOG6BCT0K.
    PhylomeDBi P0AG59.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10913-MONOMER.
    ECOL316407:JW3269-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AG59.
    PROi P0AG59.

    Gene expression databases

    Genevestigatori P0AG59.

    Family and domain databases

    HAMAPi MF_00537. Ribosomal_S14_1.
    InterProi IPR001209. Ribosomal_S14.
    IPR023036. Ribosomal_S14_bac/plaastid.
    IPR018271. Ribosomal_S14_CS.
    [Graphical view ]
    PANTHERi PTHR19836. PTHR19836. 1 hit.
    Pfami PF00253. Ribosomal_S14. 1 hit.
    [Graphical view ]
    PROSITEi PS00527. RIBOSOMAL_S14. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Yaguchi M., Roy C., Reithmeier R.A.F., Wittmann-Liebold B.
      Submitted (OCT-1982) to the PIR data bank
      Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-101.
      Strain: K12.
    2. "The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene."
      Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M.
      Nucleic Acids Res. 11:2599-2616(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry."
      Arnold R.J., Reilly J.P.
      Anal. Biochem. 269:105-112(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
      Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
    6. "All-atom homology model of the Escherichia coli 30S ribosomal subunit."
      Tung C.-S., Joseph S., Sanbonmatsu K.Y.
      Nat. Struct. Biol. 9:750-755(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.
    7. "Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement."
      Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.
      Cell 113:789-801(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS).
      Strain: MRE-600.
    8. Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES.
      Strain: MRE-600.

    Entry informationi

    Entry nameiRS14_ECOLI
    AccessioniPrimary (citable) accession number: P0AG59
    Secondary accession number(s): P02370, Q2M6X2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Ribosomal proteins
      Ribosomal proteins families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3