Reviewed, UniProtKB/Swiss-Prot P0AG51 (RL30_ECOLI)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L30

Gene names

Name:	rpmD
Ordered Locus Names:	b3302, JW3264

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	59 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Part of the 50S ribosomal subunit. HAMAP MF_01371
Miscellaneous	This protein is located near the guanosine triphosphatase center of the 50S subunit. HAMAP MF_01371
Sequence similarities	Belongs to the ribosomal protein L30P family.
Mass spectrometry	Molecular mass is 6410.3 Da from positions 2 - 59. Determined by MALDI. Ref.5

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.4

Chain

2 – 59

58

50S ribosomal protein L30 HAMAP MF_01371

PRO_0000104590

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

59

Helix Strand Turn

Details...

Beta strand

5 – 8

4

Helix

19 – 22

4

Turn

23 – 27

5

Helix

42 – 44

3

Helix

45 – 49

5

Turn

50 – 54

5

Sequences

	Sequence		Length	Mass (Da)
	P0AG51-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 08F427BBB6F820A4	FASTA	59	6,542
	10 20 30 40 50 MAKTIKITQT RSAIGRLPKH KATLLGLGLR RIGHTVERED TPAIRGMINA VSFMVKVEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene." Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M. Nucleic Acids Res. 11:2599-2616(1983) [PubMed: 6222285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The primary structure of protein L30 from Escherichia coli ribosomes." Ritter E., Wittmann-Liebold B. FEBS Lett. 60:153-155(1975) [PubMed: 776670] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-59. Strain: K.
[5]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[6]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[7]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X01563 Genomic DNA. Translation: CAA25723.1.
U18997 Genomic DNA. Translation: AAA58099.1.
U00096 Genomic DNA. Translation: AAC76327.1.
AP009048 Genomic DNA. Translation: BAE77989.1.

PIR

R5EC30. A65123.

RefSeq

AP_004488.1.
NP_417761.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P85	electron microscopy	12.30	X	2-59	[»]
1P86	electron microscopy	11.50	X	2-59	[»]
1VS6	X-ray	3.46	Y	1-59	[»]
1VS8	X-ray	3.46	Y	1-59	[»]
2AW4	X-ray	3.46	Y	2-59	[»]
2AWB	X-ray	3.46	Y	2-59	[»]
2GYA	electron microscopy	15.00	X	4-58	[»]
2GYC	electron microscopy	15.00	X	4-59	[»]
2I2T	X-ray	3.22	Z	2-58	[»]
2I2V	X-ray	3.22	Z	2-58	[»]
2J28	electron microscopy	8.00	Y	2-59	[»]
2QAM	X-ray	3.21	Y	2-59	[»]
2QAO	X-ray	3.21	Y	2-59	[»]
2QBA	X-ray	3.54	Y	2-59	[»]
2QBC	X-ray	3.54	Y	2-59	[»]
2QBE	X-ray	3.30	Y	2-59	[»]
2QBG	X-ray	3.30	Y	2-59	[»]
2QBI	X-ray	4.00	Y	2-59	[»]
2QBK	X-ray	4.00	Y	2-59	[»]
2QOV	X-ray	3.93	Y	2-59	[»]
2QOX	X-ray	3.93	Y	2-59	[»]
2QOZ	X-ray	3.50	Y	2-59	[»]
2QP1	X-ray	3.50	Y	2-59	[»]
2RDO	electron microscopy	9.10	Y	2-59	[»]
2Z4L	X-ray	4.45	Y	2-59	[»]
2Z4N	X-ray	4.45	Y	2-59	[»]
3BBX	electron microscopy	10.00	Y	2-59	[»]
3E1B	electron microscopy	-	R	1-59	[»]
3E1D	electron microscopy	-	R	1-59	[»]
3FIK	electron microscopy	6.70	Z	2-59	[»]
3I1N	X-ray	3.19	Z	1-59	[»]
3I1P	X-ray	3.19	Z	1-59	[»]
3I1R	X-ray	3.81	Z	1-59	[»]
3I1T	X-ray	3.81	Z	1-59	[»]
3I20	X-ray	3.71	Z	1-59	[»]
3I22	X-ray	3.71	Z	1-59	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0AG51.

2-D gel databases

ECO2DBASE

I011.4. 6TH EDITION.

Genome annotation databases

GeneID

947797.

GenomeReviews

Gene locus JW3264 in contig AP009048_GR.
Gene locus b3302 in contig U00096_GR.

KEGG

ecj:JW3264.
eco:b3302.

Organism-specific databases

EchoBASE

EB0881.

EcoGene

EG10888. rpmD.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AG51.

OMA

MALKITQ.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10888-MON.

Gene expression databases

Genevestigator

P0AG51.

Family and domain databases

HAMAP

MF_01371.
[Tree]

InterPro

IPR005996. Ribosomal_L30_bac-type.
IPR018038. Ribosomal_L30_CS.
IPR000517. Ribosomal_L30p/L7e_cons-reg.
[Graphical view]

Pfam

PF00327. Ribosomal_L30. 1 hit.
[Graphical view]

PIRSF

PIRSF002211. Ribosomal_L30_bac-type. 1 hit.

TIGRFAMs

TIGR01308. rpmD_bact. 1 hit.

PROSITE

PS00634. RIBOSOMAL_L30. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL30_ECOLI

Accession

Primary (citable) accession number: P0AG51
Secondary accession number(s): P02430, Q2M6W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)