Reviewed, UniProtKB/Swiss-Prot P0AG48 (RL21_ECOLI)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L21

Gene names

Name:	rplU
Ordered Locus Names:	b3186, JW3153

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	103 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein binds to 23S rRNA in the presence of protein L20. HAMAP MF_01363
Subunit structure	Part of the 50S ribosomal subunit. Contacts protein L20. HAMAP MF_01363
Sequence similarities	Belongs to the ribosomal protein L21P family.
Mass spectrometry	Molecular mass is 11562.7 Da from positions 1 - 103. Determined by MALDI. Ref.8

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 103

103

50S ribosomal protein L21 HAMAP MF_01363

PRO_0000181000

Secondary structure

1

.

.

.

.

.

.

.

.

.

103

Helix Strand Turn

Details...

Beta strand

7 – 9

3

Beta strand

14 – 16

3

Beta strand

52 – 54

3

Beta strand

56 – 58

3

Sequences

	Sequence		Length	Mass (Da)
	P0AG48-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 9221F104EC9C8CDB	FASTA	103	11,564
	10 20 30 40 50 60 MYAVFQSGGK QHRVSEGQTV RLEKLDIATG ETVEFAEVLM IANGEEVKIG VPFVDGGVIK 70 80 90 100 AEVVAHGRGE KVKIVKFRRR KHYRKQQGHR QWFTDVKITG ISA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Amino acid sequence of the ribosomal protein L21 of Escherichia coli." Heiland I., Wittmann-Liebold B. Biochemistry 18:4605-4612(1979) [PubMed: 387076] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Cloning and nucleotide sequencing of the genes, rpIU and rpmA, for ribosomal proteins L21 and L27 of Escherichia coli." Jeong J.H., Kitakawa M.S., Isono S., Isono K. DNA Seq. 4:59-67(1993) [PubMed: 8312607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F. Submitted (SEP-1994) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-13. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The use of 2-iminothiolane as an RNA-protein cross-linking agent in Escherichia coli ribosomes, and the localisation on 23S RNA of sites cross-linked to proteins L4, L6, L21, L23, L27 and L29." Wower I., Wower J., Meinke M., Brimacombe R. Nucleic Acids Res. 9:4285-4302(1981) [PubMed: 6170935] [Abstract] Cited for: CROSS-LINKING TO 23S RRNA. Strain: MRE-600.
[7]	"Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli." Walleczek J., Martin T., Redl B., Stoeffler-Meilicke M., Stoeffler G. Biochemistry 28:4099-4105(1989) [PubMed: 2665813] [Abstract] Cited for: CROSS-LINKING TO L20.
[8]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[9]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D13267 Genomic DNA. Translation: BAA02525.1.
U18997 Genomic DNA. Translation: AAA57987.1.
U00096 Genomic DNA. Translation: AAC76218.1.
AP009048 Genomic DNA. Translation: BAE77230.1.

PIR

R5EC21. JS0766.

RefSeq

AP_003729.1.
NP_417653.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VS6	X-ray	3.46	R	1-103	[»]
1VS8	X-ray	3.46	R	1-103	[»]
2AW4	X-ray	3.46	R	1-103	[»]
2AWB	X-ray	3.46	R	1-103	[»]
2I2T	X-ray	3.22	R	1-103	[»]
2I2V	X-ray	3.22	R	1-103	[»]
2J28	electron microscopy	8.00	R	1-103	[»]
2QAM	X-ray	3.21	R	1-103	[»]
2QAO	X-ray	3.21	R	1-103	[»]
2QBA	X-ray	3.54	R	1-103	[»]
2QBC	X-ray	3.54	R	1-103	[»]
2QBE	X-ray	3.30	R	1-103	[»]
2QBG	X-ray	3.30	R	1-103	[»]
2QBI	X-ray	4.00	R	1-103	[»]
2QBK	X-ray	4.00	R	1-103	[»]
2QOV	X-ray	3.93	R	1-103	[»]
2QOX	X-ray	3.93	R	1-103	[»]
2QOZ	X-ray	3.50	R	1-103	[»]
2QP1	X-ray	3.50	R	1-103	[»]
2RDO	electron microscopy	9.10	R	1-103	[»]
2Z4L	X-ray	4.45	R	1-103	[»]
2Z4N	X-ray	4.45	R	1-103	[»]
3BBX	electron microscopy	10.00	R	1-103	[»]
3E1B	electron microscopy	-	K	1-103	[»]
3E1D	electron microscopy	-	K	1-103	[»]
3FIK	electron microscopy	6.70	R	1-103	[»]
3I1N	X-ray	3.19	R	1-103	[»]
3I1P	X-ray	3.19	R	1-103	[»]
3I1R	X-ray	3.81	R	1-103	[»]
3I1T	X-ray	3.81	R	1-103	[»]
3I20	X-ray	3.71	R	1-103	[»]
3I22	X-ray	3.71	R	1-103	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0AG48.

2-D gel databases

SWISS-2DPAGE

P0AG48.

ECO2DBASE

I013.2. 6TH EDITION.

Genome annotation databases

GeneID

949057.

GenomeReviews

Gene locus JW3153 in contig AP009048_GR.
Gene locus b3186 in contig U00096_GR.

KEGG

ecj:JW3153.
eco:b3186.

Organism-specific databases

EchoBASE

EB4295.

EcoGene

EG50001. rplU.

CMR

Search...

Phylogenomic databases

eggNOG

COG0261.

HOGENOM

HBG706775.

OMA

YRRKQGH.

Enzyme and pathway databases

BioCyc

EcoCyc:EG50001-MONOMER.
ECOL168927:B3186-MONOMER.

Gene expression databases

Genevestigator

P0AG48.

Family and domain databases

HAMAP

MF_01363. Ribosomal_L21.
[Tree]

InterPro

IPR001787. Ribosomal_L21.
IPR018258. Ribosomal_L21_CS.
[Graphical view]

PANTHER

PTHR21349. Ribosomal_L21p. 1 hit.

Pfam

PF00829. Ribosomal_L21p. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00061. L21. 1 hit.

PROSITE

PS01169. RIBOSOMAL_L21. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL21_ECOLI

Accession

Primary (citable) accession number: P0AG48
Secondary accession number(s): P02422, Q2M926

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)