50S ribosomal protein L17 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0AG44 (RL17_ECOLI)

Last modified June 16, 2009. Version 40. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
50S ribosomal protein L17

Gene names

Name:	rplQ
Ordered Locus Names:	b3294, JW3256

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	127 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Requires L15 for assembly into the 50S subunit. HAMAP MF_01368
Subunit structure	Part of the 50S ribosomal subunit. Contacts protein L32. Ref.6
Sequence similarities	Belongs to the ribosomal protein L17P family.
Mass spectrometry	Molecular mass is 14364.7 Da from positions 1 - 127. Determined by MALDI. Ref.8

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Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 127

127

50S ribosomal protein L17 HAMAP MF_01368

PRO_0000175524

Secondary structure

127

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0AG44-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: D96DF17C07013A11
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FASTA

127

14,365

        10         20         30         40         50         60 
MRHRKSGRQL NRNSSHRQAM FRNMAGSLVR HEIIKTTLPK AKELRRVVEP LITLAKTDSV 

        70         80         90        100        110        120 
ANRRLAFART RDNEIVAKLF NELGPRFASR AGGYTRILKC GFRAGDNAPM AYIELVDRSE 


KAEAAAE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of protein L17 from the Escherichia coli ribosome." Rombauts W., Feytons V., Wittmann-Liebold B. FEBS Lett. 149:320-327(1982) Cited for: PROTEIN SEQUENCE.
[2]	"Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli: a second regulatory binding site for protein S4?" Meek D.W., Hayward R.S. Nucleic Acids Res. 12:5813-5821(1984) [PubMed: 6379605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli." Bedwell D.M., Davis G.R., Gosink M., Post L.E., Nomura M., Kestler H., Zengel J.M., Lindahl L. Nucleic Acids Res. 13:3891-3903(1985) [PubMed: 2989779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Incorporation of six additional proteins to complete the assembly map of the 50 S subunit from Escherichia coli ribosomes." Herold M., Nierhaus K.H. J. Biol. Chem. 262:8826-8833(1987) [PubMed: 3298242] [Abstract] Cited for: ASSEMBLY MAP OF THE 50S SUBUNIT. Strain: K12.
[7]	"Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli." Walleczek J., Martin T., Redl B., Stoeffler-Meilicke M., Stoeffler G. Biochemistry 28:4099-4105(1989) [PubMed: 2665813] [Abstract] Cited for: CROSS-LINKING TO L32.
[8]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[9]	"Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement." Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J. Cell 113:789-801(2003) [PubMed: 12809609] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). Strain: MRE-600.
[10]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X02543 Genomic DNA. Translation: CAA26396.1.
J01685 Genomic DNA. Translation: AAA24578.1.
X00766 Genomic DNA. Translation: CAA25338.1.
U18997 Genomic DNA. Translation: AAA58091.1.
U00096 Genomic DNA. Translation: AAC76319.1.
AP009048 Genomic DNA. Translation: BAE77997.1.

PIR

R5EC17. B22884.

RefSeq

AP_004496.1.
NP_417753.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P85	electron microscopy	12.30	L	1-127	[»]
1P86	electron microscopy	11.50	L	1-127	[»]
1VS6	X-ray	3.46	N	1-127	[»]
1VS8	X-ray	3.46	N	1-127	[»]
2AW4	X-ray	3.46	N	1-127	[»]
2AWB	X-ray	3.46	N	1-127	[»]
2GYA	electron microscopy	2.00	L	3-116	[»]
2GYC	electron microscopy	-	L	3-116	[»]
2I2T	X-ray	3.22	N	1-127	[»]
2I2V	X-ray	3.22	N	1-127	[»]
2J28	electron microscopy	8.00	N	1-127	[»]
2QAM	X-ray	3.21	N	1-127	[»]
2QAO	X-ray	3.21	N	1-127	[»]
2QBA	X-ray	3.54	N	1-127	[»]
2QBC	X-ray	3.54	N	1-127	[»]
2QBE	X-ray	3.30	N	1-127	[»]
2QBG	X-ray	3.30	N	1-127	[»]
2QBI	X-ray	4.00	N	1-127	[»]
2QBK	X-ray	4.00	N	1-127	[»]
2QOV	X-ray	3.93	N	1-127	[»]
2QOX	X-ray	3.93	N	1-127	[»]
2QOZ	X-ray	3.50	N	1-127	[»]
2QP1	X-ray	3.50	N	1-127	[»]
2RDO	electron microscopy	9.10	N	1-127	[»]
2Z4L	X-ray	4.45	N	1-127	[»]
2Z4N	X-ray	4.45	N	1-127	[»]
3BBX	electron microscopy	-	N	1-127	[»]
3FIK	electron microscopy	-	N	1-120	[»]

ModBase

Search...

2-D gel databases

ECO2DBASE

I014.9. 6TH EDITION.

Genome annotation databases

GeneID

947784.

GenomeReviews

Gene locus JW3256 in contig AP009048_GR.
Gene locus b3294 in contig U00096_GR.

KEGG

ecj:JW3256.
eco:b3294.

Organism-specific databases

EchoBASE

EB0871.

EcoGene

EG10878. rplQ.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AG44.

OMA

P0AG44. TGDKAPM.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10878-MON.

Family and domain databases

HAMAP

MF_01368.
[Tree]

InterPro

IPR000456. Ribosomal_L17.
[Graphical view]

Gene3D

G3DSA:3.90.1030.10. Ribosomal_L17. 1 hit.

PANTHER

PTHR14413. Ribosomal_L17. 1 hit.

Pfam

PF01196. Ribosomal_L17. 1 hit.
[Graphical view]

ProDom

PD004277. Ribosomal_L17. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00059. L17. 1 hit.

PROSITE

PS01167. RIBOSOMAL_L17. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

RL17_ECOLI

Accession

Primary (citable) accession number: P0AG44
Secondary accession number(s): P02416, Q2M6V9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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