P0AG43 (RIBF_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin biosynthesis protein RibF

Including the following 2 domains:

1. Riboflavin kinase
   EC=2.7.1.26
   Alternative name(s):
   Flavokinase
2. FMN adenylyltransferase
   EC=2.7.7.2
   Alternative name(s):
   FAD pyrophosphorylase
   FAD synthase

Gene names

Name:	ribF
Ordered Locus Names:	SF0021, S0024

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + riboflavin = ADP + FMN. ATP + FMN = diphosphate + FAD.
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Sequence similarities	Belongs to the RibF family.

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Kinase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	FAD biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway FMN biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW FMN adenylyltransferase activity Inferred from electronic annotation. Source: EC riboflavin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Riboflavin biosynthesis protein RibF		PRO_0000194143

Sequences

	Sequence		Length	Mass (Da)
	P0AG43 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: C0B2EF5499CD30FE	FASTA	313	34,734
	10 20 30 40 50 60 MKLIRGIHNL SQAPQEGCVL TIGNFDGVHR GHRALLQGLQ EEGRKRNLPV MVMLFEPQPL 70 80 90 100 110 120 ELFATDKAPA RLTRLREKLR YLAECGVDYV LCVRFDRRFA ALTAQNFISD LLVKHLRVKF 130 140 150 160 170 180 LAVGDDFRFG AGREGDFLLL QKAGMEYGFD ITSTQTFCEG GVRISSTAVR QALADDNLAL 190 200 210 220 230 240 AESLLGHPFA ISGRVVHGDE LGRTIGFPTA NVPLRRQVSP VKGVYAVEVL GLGEKPLPGV 250 260 270 280 290 300 ANIGTRPTVA GIRQQLEVHL LDVAMDLYGR HIQVVLRKKI RNEQRFASLD ELKAQIARDE 310 LTAREFFGLT KPA

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN41687.1. AE014073 Genomic DNA. Translation: AAP15569.1.
RefSeq	NP_705980.1. NC_004337.2. NP_835764.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0AG43.
SMR	P0AG43. Positions 19-306.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF0021.
Proteomic databases
PaxDb	P0AG43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN41687; AAN41687; SF0021. AAP15569; AAP15569; S0024.
GeneID	1024592. 1076481.
KEGG	sfl:SF0021. sfx:S0024.
PATRIC	18700966. VBIShiFle31049_0025.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0196.
HOGENOM	HOG000006845.
KO	K11753.
OMA	FHFGRGR.
ProtClustDB	PRK05627.
Enzyme and pathway databases
UniPathway	UPA00276; UER00406. UPA00277; UER00407.
Family and domain databases
Gene3D	2.40.30.30. 1 hit. 3.40.50.620. 1 hit.
InterPro	IPR015864. FAD_synthase. IPR023468. Riboflavin_kinase. IPR002606. Riboflavin_kinase_bac. IPR015865. Riboflavin_kinase_bac/euk. IPR023465. Riboflavin_kinase_domain. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR22749. PTHR22749. 1 hit.
Pfam	PF06574. FAD_syn. 1 hit. PF01687. Flavokinase. 1 hit. [Graphical view]
PIRSF	PIRSF004491. FAD_Synth. 1 hit.
SMART	SM00904. Flavokinase. 1 hit. [Graphical view]
SUPFAM	SSF82114. Riboflavin_kinase. 1 hit.
TIGRFAMs	TIGR00083. ribF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBF_SHIFL

Accession

Primary (citable) accession number: P0AG43
Secondary accession number(s): P08391, P75621

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program