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P0AG40 (RIBF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Riboflavin biosynthesis protein RibF

Including the following 2 domains:

  1. Riboflavin kinase
    EC=2.7.1.26
    Alternative name(s):
    Flavokinase
  2. FMN adenylyltransferase
    EC=2.7.7.2
    Alternative name(s):
    FAD pyrophosphorylase
    FAD synthase
Gene names
Name:ribF
Synonyms:yaaC
Ordered Locus Names:b0025, JW0023
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + riboflavin = ADP + FMN.

ATP + FMN = diphosphate + FAD.

Pathway

Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.

Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.

Sequence similarities

Belongs to the RibF family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

aceEP0AFG81EBI-542969,EBI-542683

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Riboflavin biosynthesis protein RibF
PRO_0000194137

Experimental info

Sequence conflict1081I → V in AAA24605. Ref.1
Sequence conflict1081I → V no nucleotide entry Ref.2
Sequence conflict128 – 16942RFGAG…SSTAV → PLALVVKAISCYYRKLAWNT ASISPVRKLFAEVACASAAR L in AAA24605. Ref.1
Sequence conflict128 – 16942RFGAG…SSTAV → PLALVVKAISCYYRKLAWNT ASISPVRKLFAEVACASAAR L no nucleotide entry Ref.2
Sequence conflict2141L → P in AAA24605. Ref.1
Sequence conflict2141L → P no nucleotide entry Ref.2

Sequences

Sequence LengthMass (Da)Tools
P0AG40 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: C0B2EF5499CD30FE

FASTA31334,734
        10         20         30         40         50         60 
MKLIRGIHNL SQAPQEGCVL TIGNFDGVHR GHRALLQGLQ EEGRKRNLPV MVMLFEPQPL 

        70         80         90        100        110        120 
ELFATDKAPA RLTRLREKLR YLAECGVDYV LCVRFDRRFA ALTAQNFISD LLVKHLRVKF 

       130        140        150        160        170        180 
LAVGDDFRFG AGREGDFLLL QKAGMEYGFD ITSTQTFCEG GVRISSTAVR QALADDNLAL 

       190        200        210        220        230        240 
AESLLGHPFA ISGRVVHGDE LGRTIGFPTA NVPLRRQVSP VKGVYAVEVL GLGEKPLPGV 

       250        260        270        280        290        300 
ANIGTRPTVA GIRQQLEVHL LDVAMDLYGR HIQVVLRKKI RNEQRFASLD ELKAQIARDE 

       310 
LTAREFFGLT KPA 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon."
Kamio Y., Lin C.-K., Regue M., Wu H.C.
J. Biol. Chem. 260:5616-5620(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 108; 128-169 AND 214.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M10428 Genomic DNA. Translation: AAA24605.1.
U00096 Genomic DNA. Translation: AAC73136.1.
AP009048 Genomic DNA. Translation: BAB96594.2.
PIRQQECIL. A64723.
RefSeqNP_414566.1. NC_000913.3.
YP_488331.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AG40.
SMRP0AG40. Positions 19-306.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35789N.
IntActP0AG40. 10 interactions.
MINTMINT-1256514.
STRING511145.b0025.

Proteomic databases

PaxDbP0AG40.
PRIDEP0AG40.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73136; AAC73136; b0025.
BAB96594; BAB96594; BAB96594.
GeneID12932854.
949129.
KEGGecj:Y75_p0025.
eco:b0025.
PATRIC32115141. VBIEscCol129921_0022.

Organism-specific databases

EchoBASEEB1071.
EcoGeneEG11079. ribF.

Phylogenomic databases

eggNOGCOG0196.
HOGENOMHOG000006845.
KOK11753.
OMAGVASFGC.
OrthoDBEOG6QP0ZV.
PhylomeDBP0AG40.

Enzyme and pathway databases

BioCycEcoCyc:RIBF-MONOMER.
ECOL316407:JW0023-MONOMER.
MetaCyc:RIBF-MONOMER.
UniPathwayUPA00276; UER00406.
UPA00277; UER00407.

Gene expression databases

GenevestigatorP0AG40.

Family and domain databases

Gene3D2.40.30.30. 1 hit.
3.40.50.620. 1 hit.
InterProIPR015864. FAD_synthase.
IPR023468. Riboflavin_kinase.
IPR002606. Riboflavin_kinase_bac.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR22749. PTHR22749. 1 hit.
PfamPF06574. FAD_syn. 1 hit.
PF01687. Flavokinase. 1 hit.
[Graphical view]
PIRSFPIRSF004491. FAD_Synth. 1 hit.
SMARTSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMSSF82114. SSF82114. 1 hit.
TIGRFAMsTIGR00083. ribF. 1 hit.
ProtoNetSearch...

Other

PROP0AG40.

Entry information

Entry nameRIBF_ECOLI
AccessionPrimary (citable) accession number: P0AG40
Secondary accession number(s): P08391, P75621
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene