SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0AG40

- RIBF_ECOLI

UniProt

P0AG40 - RIBF_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Riboflavin biosynthesis protein RibF
Gene
ribF, yaaC, b0025, JW0023
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + riboflavin = ADP + FMN.
ATP + FMN = diphosphate + FAD.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. FMN adenylyltransferase activity Source: EcoCyc
  3. riboflavin kinase activity Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  1. FAD biosynthetic process Source: UniProtKB-UniPathway
  2. FMN biosynthetic process Source: UniProtKB-UniPathway
  3. riboflavin biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:RIBF-MONOMER.
ECOL316407:JW0023-MONOMER.
MetaCyc:RIBF-MONOMER.
UniPathwayiUPA00276; UER00406.
UPA00277; UER00407.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin biosynthesis protein RibF
Including the following 2 domains:
Riboflavin kinase (EC:2.7.1.26)
Alternative name(s):
Flavokinase
FMN adenylyltransferase (EC:2.7.7.2)
Alternative name(s):
FAD pyrophosphorylase
FAD synthase
Gene namesi
Name:ribF
Synonyms:yaaC
Ordered Locus Names:b0025, JW0023
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11079. ribF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Riboflavin biosynthesis protein RibF
PRO_0000194137Add
BLAST

Proteomic databases

PaxDbiP0AG40.
PRIDEiP0AG40.

Expressioni

Gene expression databases

GenevestigatoriP0AG40.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
aceEP0AFG81EBI-542969,EBI-542683

Protein-protein interaction databases

DIPiDIP-35789N.
IntActiP0AG40. 10 interactions.
MINTiMINT-1256514.
STRINGi511145.b0025.

Structurei

3D structure databases

ProteinModelPortaliP0AG40.
SMRiP0AG40. Positions 19-306.

Family & Domainsi

Sequence similaritiesi

Belongs to the RibF family.

Phylogenomic databases

eggNOGiCOG0196.
HOGENOMiHOG000006845.
KOiK11753.
OMAiGVASFGC.
OrthoDBiEOG6QP0ZV.
PhylomeDBiP0AG40.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR015864. FAD_synthase.
IPR023468. Riboflavin_kinase.
IPR002606. Riboflavin_kinase_bac.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 1 hit.
PfamiPF06574. FAD_syn. 1 hit.
PF01687. Flavokinase. 1 hit.
[Graphical view]
PIRSFiPIRSF004491. FAD_Synth. 1 hit.
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.
TIGRFAMsiTIGR00083. ribF. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG40-1 [UniParc]FASTAAdd to Basket

« Hide

MKLIRGIHNL SQAPQEGCVL TIGNFDGVHR GHRALLQGLQ EEGRKRNLPV    50
MVMLFEPQPL ELFATDKAPA RLTRLREKLR YLAECGVDYV LCVRFDRRFA 100
ALTAQNFISD LLVKHLRVKF LAVGDDFRFG AGREGDFLLL QKAGMEYGFD 150
ITSTQTFCEG GVRISSTAVR QALADDNLAL AESLLGHPFA ISGRVVHGDE 200
LGRTIGFPTA NVPLRRQVSP VKGVYAVEVL GLGEKPLPGV ANIGTRPTVA 250
GIRQQLEVHL LDVAMDLYGR HIQVVLRKKI RNEQRFASLD ELKAQIARDE 300
LTAREFFGLT KPA 313
Length:313
Mass (Da):34,734
Last modified:December 20, 2005 - v1
Checksum:iC0B2EF5499CD30FE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081I → V in AAA24605. 1 Publication
Sequence conflicti108 – 1081I → V no nucleotide entry 1 Publication
Sequence conflicti128 – 16942RFGAG…SSTAV → PLALVVKAISCYYRKLAWNT ASISPVRKLFAEVACASAAR L in AAA24605. 1 Publication
Add
BLAST
Sequence conflicti128 – 16942RFGAG…SSTAV → PLALVVKAISCYYRKLAWNT ASISPVRKLFAEVACASAAR L no nucleotide entry 1 Publication
Add
BLAST
Sequence conflicti214 – 2141L → P in AAA24605. 1 Publication
Sequence conflicti214 – 2141L → P no nucleotide entry 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10428 Genomic DNA. Translation: AAA24605.1.
U00096 Genomic DNA. Translation: AAC73136.1.
AP009048 Genomic DNA. Translation: BAB96594.2.
PIRiA64723. QQECIL.
RefSeqiNP_414566.1. NC_000913.3.
YP_488331.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73136; AAC73136; b0025.
BAB96594; BAB96594; BAB96594.
GeneIDi12932854.
949129.
KEGGiecj:Y75_p0025.
eco:b0025.
PATRICi32115141. VBIEscCol129921_0022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10428 Genomic DNA. Translation: AAA24605.1 .
U00096 Genomic DNA. Translation: AAC73136.1 .
AP009048 Genomic DNA. Translation: BAB96594.2 .
PIRi A64723. QQECIL.
RefSeqi NP_414566.1. NC_000913.3.
YP_488331.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AG40.
SMRi P0AG40. Positions 19-306.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-35789N.
IntActi P0AG40. 10 interactions.
MINTi MINT-1256514.
STRINGi 511145.b0025.

Proteomic databases

PaxDbi P0AG40.
PRIDEi P0AG40.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73136 ; AAC73136 ; b0025 .
BAB96594 ; BAB96594 ; BAB96594 .
GeneIDi 12932854.
949129.
KEGGi ecj:Y75_p0025.
eco:b0025.
PATRICi 32115141. VBIEscCol129921_0022.

Organism-specific databases

EchoBASEi EB1071.
EcoGenei EG11079. ribF.

Phylogenomic databases

eggNOGi COG0196.
HOGENOMi HOG000006845.
KOi K11753.
OMAi GVASFGC.
OrthoDBi EOG6QP0ZV.
PhylomeDBi P0AG40.

Enzyme and pathway databases

UniPathwayi UPA00276 ; UER00406 .
UPA00277 ; UER00407 .
BioCyci EcoCyc:RIBF-MONOMER.
ECOL316407:JW0023-MONOMER.
MetaCyc:RIBF-MONOMER.

Miscellaneous databases

PROi P0AG40.

Gene expression databases

Genevestigatori P0AG40.

Family and domain databases

Gene3Di 2.40.30.30. 1 hit.
3.40.50.620. 1 hit.
InterProi IPR015864. FAD_synthase.
IPR023468. Riboflavin_kinase.
IPR002606. Riboflavin_kinase_bac.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR22749. PTHR22749. 1 hit.
Pfami PF06574. FAD_syn. 1 hit.
PF01687. Flavokinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF004491. FAD_Synth. 1 hit.
SMARTi SM00904. Flavokinase. 1 hit.
[Graphical view ]
SUPFAMi SSF82114. SSF82114. 1 hit.
TIGRFAMsi TIGR00083. ribF. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon."
    Kamio Y., Lin C.-K., Regue M., Wu H.C.
    J. Biol. Chem. 260:5616-5620(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
    Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
    Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 108; 128-169 AND 214.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiRIBF_ECOLI
AccessioniPrimary (citable) accession number: P0AG40
Secondary accession number(s): P08391, P75621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi