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Protein

Bifunctional riboflavin kinase/FMN adenylyltransferase

Gene

ribF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of riboflavin to FMN followed by the adenylation of FMN to FAD.By similarity

Catalytic activityi

ATP + riboflavin = ADP + FMN.By similarity
ATP + FMN = diphosphate + FAD.By similarity

Pathwayi: FAD biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FAD from FMN.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF)
This subpathway is part of the pathway FAD biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FAD from FMN, the pathway FAD biosynthesis and in Cofactor biosynthesis.

Pathwayi: FMN biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FMN from riboflavin (ATP route).By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF)
This subpathway is part of the pathway FMN biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FMN from riboflavin (ATP route), the pathway FMN biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • FMN adenylyltransferase activity Source: EcoCyc
  • riboflavin kinase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionKinase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
LigandATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:RIBF-MONOMER
MetaCyc:RIBF-MONOMER
UniPathwayiUPA00276; UER00406
UPA00277; UER00407

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional riboflavin kinase/FMN adenylyltransferaseBy similarity
Alternative name(s):
Riboflavin biosynthesis protein RibFBy similarity
Including the following 2 domains:
Riboflavin kinaseBy similarity (EC:2.7.1.26By similarity)
Alternative name(s):
FlavokinaseBy similarity
FMN adenylyltransferaseBy similarity (EC:2.7.7.2By similarity)
Alternative name(s):
FAD pyrophosphorylaseBy similarity
FAD synthaseBy similarity
Gene namesi
Name:ribF
Synonyms:yaaC
Ordered Locus Names:b0025, JW0023
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11079 ribF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941371 – 313Bifunctional riboflavin kinase/FMN adenylyltransferaseAdd BLAST313

Proteomic databases

PaxDbiP0AG40
PRIDEiP0AG40

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
aceEP0AFG82EBI-542969,EBI-542683

Protein-protein interaction databases

BioGridi4261848, 7 interactors
DIPiDIP-35789N
IntActiP0AG40, 10 interactors
STRINGi316385.ECDH10B_0026

Structurei

3D structure databases

ProteinModelPortaliP0AG40
SMRiP0AG40
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RibF family.Curated

Phylogenomic databases

eggNOGiENOG4105DTN Bacteria
COG0196 LUCA
HOGENOMiHOG000006845
InParanoidiP0AG40
KOiK11753
OMAiHRGHQAI
PhylomeDBiP0AG40

Family and domain databases

Gene3Di2.40.30.30, 1 hit
3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR015864 FAD_synthase
IPR023468 Riboflavin_kinase
IPR002606 Riboflavin_kinase_bac
IPR015865 Riboflavin_kinase_bac/euk
IPR023465 Riboflavin_kinase_dom_sf
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR22749 PTHR22749, 1 hit
PfamiView protein in Pfam
PF06574 FAD_syn, 1 hit
PF01687 Flavokinase, 1 hit
PIRSFiPIRSF004491 FAD_Synth, 1 hit
SMARTiView protein in SMART
SM00904 Flavokinase, 1 hit
SUPFAMiSSF82114 SSF82114, 1 hit
TIGRFAMsiTIGR00083 ribF, 1 hit

Sequencei

Sequence statusi: Complete.

P0AG40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIRGIHNL SQAPQEGCVL TIGNFDGVHR GHRALLQGLQ EEGRKRNLPV
60 70 80 90 100
MVMLFEPQPL ELFATDKAPA RLTRLREKLR YLAECGVDYV LCVRFDRRFA
110 120 130 140 150
ALTAQNFISD LLVKHLRVKF LAVGDDFRFG AGREGDFLLL QKAGMEYGFD
160 170 180 190 200
ITSTQTFCEG GVRISSTAVR QALADDNLAL AESLLGHPFA ISGRVVHGDE
210 220 230 240 250
LGRTIGFPTA NVPLRRQVSP VKGVYAVEVL GLGEKPLPGV ANIGTRPTVA
260 270 280 290 300
GIRQQLEVHL LDVAMDLYGR HIQVVLRKKI RNEQRFASLD ELKAQIARDE
310
LTAREFFGLT KPA
Length:313
Mass (Da):34,734
Last modified:December 20, 2005 - v1
Checksum:iC0B2EF5499CD30FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108I → V in AAA24605 (PubMed:2985604).Curated1
Sequence conflicti108I → V no nucleotide entry (PubMed:1630901).Curated1
Sequence conflicti128 – 169RFGAG…SSTAV → PLALVVKAISCYYRKLAWNT ASISPVRKLFAEVACASAAR L in AAA24605 (PubMed:2985604).CuratedAdd BLAST42
Sequence conflicti128 – 169RFGAG…SSTAV → PLALVVKAISCYYRKLAWNT ASISPVRKLFAEVACASAAR L no nucleotide entry (PubMed:1630901).CuratedAdd BLAST42
Sequence conflicti214L → P in AAA24605 (PubMed:2985604).Curated1
Sequence conflicti214L → P no nucleotide entry (PubMed:1630901).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10428 Genomic DNA Translation: AAA24605.1
U00096 Genomic DNA Translation: AAC73136.1
AP009048 Genomic DNA Translation: BAB96594.2
PIRiA64723 QQECIL
RefSeqiNP_414566.1, NC_000913.3
WP_000767329.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73136; AAC73136; b0025
BAB96594; BAB96594; BAB96594
GeneIDi949129
KEGGiecj:JW0023
eco:b0025
PATRICifig|1411691.4.peg.2260

Similar proteinsi

Entry informationi

Entry nameiRIBF_ECOLI
AccessioniPrimary (citable) accession number: P0AG40
Secondary accession number(s): P08391, P75621
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: December 20, 2005
Last modified: April 25, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health