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Protein

Riboflavin biosynthesis protein RibF

Gene

ribF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + riboflavin = ADP + FMN.
ATP + FMN = diphosphate + FAD.

Pathwayi: FAD biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FAD from FMN.
Proteins known to be involved in this subpathway in this organism are:
  1. Riboflavin biosynthesis protein RibF (ribF)
This subpathway is part of the pathway FAD biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FAD from FMN, the pathway FAD biosynthesis and in Cofactor biosynthesis.

Pathwayi: FMN biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FMN from riboflavin (ATP route).
Proteins known to be involved in this subpathway in this organism are:
  1. Riboflavin biosynthesis protein RibF (ribF)
This subpathway is part of the pathway FMN biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FMN from riboflavin (ATP route), the pathway FMN biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • FMN adenylyltransferase activity Source: EcoCyc
  • riboflavin kinase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:RIBF-MONOMER.
ECOL316407:JW0023-MONOMER.
MetaCyc:RIBF-MONOMER.
UniPathwayiUPA00276; UER00406.
UPA00277; UER00407.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin biosynthesis protein RibF
Including the following 2 domains:
Riboflavin kinase (EC:2.7.1.26)
Alternative name(s):
Flavokinase
FMN adenylyltransferase (EC:2.7.7.2)
Alternative name(s):
FAD pyrophosphorylase
FAD synthase
Gene namesi
Name:ribF
Synonyms:yaaC
Ordered Locus Names:b0025, JW0023
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11079. ribF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941371 – 313Riboflavin biosynthesis protein RibFAdd BLAST313

Proteomic databases

PaxDbiP0AG40.
PRIDEiP0AG40.

Interactioni

Protein-protein interaction databases

BioGridi4261848. 3 interactors.
DIPiDIP-35789N.
IntActiP0AG40. 10 interactors.
MINTiMINT-1256514.
STRINGi511145.b0025.

Structurei

3D structure databases

ProteinModelPortaliP0AG40.
SMRiP0AG40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RibF family.Curated

Phylogenomic databases

eggNOGiENOG4105DTN. Bacteria.
COG0196. LUCA.
HOGENOMiHOG000006845.
InParanoidiP0AG40.
KOiK11753.
OMAiFPTANMR.
PhylomeDBiP0AG40.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR015864. FAD_synthase.
IPR023468. Riboflavin_kinase.
IPR002606. Riboflavin_kinase_bac.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 1 hit.
PfamiPF06574. FAD_syn. 1 hit.
PF01687. Flavokinase. 1 hit.
[Graphical view]
PIRSFiPIRSF004491. FAD_Synth. 1 hit.
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.
TIGRFAMsiTIGR00083. ribF. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIRGIHNL SQAPQEGCVL TIGNFDGVHR GHRALLQGLQ EEGRKRNLPV
60 70 80 90 100
MVMLFEPQPL ELFATDKAPA RLTRLREKLR YLAECGVDYV LCVRFDRRFA
110 120 130 140 150
ALTAQNFISD LLVKHLRVKF LAVGDDFRFG AGREGDFLLL QKAGMEYGFD
160 170 180 190 200
ITSTQTFCEG GVRISSTAVR QALADDNLAL AESLLGHPFA ISGRVVHGDE
210 220 230 240 250
LGRTIGFPTA NVPLRRQVSP VKGVYAVEVL GLGEKPLPGV ANIGTRPTVA
260 270 280 290 300
GIRQQLEVHL LDVAMDLYGR HIQVVLRKKI RNEQRFASLD ELKAQIARDE
310
LTAREFFGLT KPA
Length:313
Mass (Da):34,734
Last modified:December 20, 2005 - v1
Checksum:iC0B2EF5499CD30FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108I → V in AAA24605 (PubMed:2985604).Curated1
Sequence conflicti108I → V no nucleotide entry (PubMed:1630901).Curated1
Sequence conflicti128 – 169RFGAG…SSTAV → PLALVVKAISCYYRKLAWNT ASISPVRKLFAEVACASAAR L in AAA24605 (PubMed:2985604).CuratedAdd BLAST42
Sequence conflicti128 – 169RFGAG…SSTAV → PLALVVKAISCYYRKLAWNT ASISPVRKLFAEVACASAAR L no nucleotide entry (PubMed:1630901).CuratedAdd BLAST42
Sequence conflicti214L → P in AAA24605 (PubMed:2985604).Curated1
Sequence conflicti214L → P no nucleotide entry (PubMed:1630901).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10428 Genomic DNA. Translation: AAA24605.1.
U00096 Genomic DNA. Translation: AAC73136.1.
AP009048 Genomic DNA. Translation: BAB96594.2.
PIRiA64723. QQECIL.
RefSeqiNP_414566.1. NC_000913.3.
WP_000767329.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73136; AAC73136; b0025.
BAB96594; BAB96594; BAB96594.
GeneIDi949129.
KEGGiecj:JW0023.
eco:b0025.
PATRICi32115141. VBIEscCol129921_0022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10428 Genomic DNA. Translation: AAA24605.1.
U00096 Genomic DNA. Translation: AAC73136.1.
AP009048 Genomic DNA. Translation: BAB96594.2.
PIRiA64723. QQECIL.
RefSeqiNP_414566.1. NC_000913.3.
WP_000767329.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AG40.
SMRiP0AG40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261848. 3 interactors.
DIPiDIP-35789N.
IntActiP0AG40. 10 interactors.
MINTiMINT-1256514.
STRINGi511145.b0025.

Proteomic databases

PaxDbiP0AG40.
PRIDEiP0AG40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73136; AAC73136; b0025.
BAB96594; BAB96594; BAB96594.
GeneIDi949129.
KEGGiecj:JW0023.
eco:b0025.
PATRICi32115141. VBIEscCol129921_0022.

Organism-specific databases

EchoBASEiEB1071.
EcoGeneiEG11079. ribF.

Phylogenomic databases

eggNOGiENOG4105DTN. Bacteria.
COG0196. LUCA.
HOGENOMiHOG000006845.
InParanoidiP0AG40.
KOiK11753.
OMAiFPTANMR.
PhylomeDBiP0AG40.

Enzyme and pathway databases

UniPathwayiUPA00276; UER00406.
UPA00277; UER00407.
BioCyciEcoCyc:RIBF-MONOMER.
ECOL316407:JW0023-MONOMER.
MetaCyc:RIBF-MONOMER.

Miscellaneous databases

PROiP0AG40.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR015864. FAD_synthase.
IPR023468. Riboflavin_kinase.
IPR002606. Riboflavin_kinase_bac.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 1 hit.
PfamiPF06574. FAD_syn. 1 hit.
PF01687. Flavokinase. 1 hit.
[Graphical view]
PIRSFiPIRSF004491. FAD_Synth. 1 hit.
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.
TIGRFAMsiTIGR00083. ribF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRIBF_ECOLI
AccessioniPrimary (citable) accession number: P0AG40
Secondary accession number(s): P08391, P75621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.