Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0AG33

- RHO_SHIFL

UniProt

P0AG33 - RHO_SHIFL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Transcription termination factor Rho

Gene

rho

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei212 – 2121ATPUniRule annotation
Sitei326 – 3261RNA-binding 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi169 – 1746ATPUniRule annotation
Nucleotide bindingi181 – 1866ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. helicase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: UniProtKB-HAMAP
  4. RNA-dependent ATPase activity Source: InterPro

GO - Biological processi

  1. DNA-templated transcription, termination Source: UniProtKB-HAMAP
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation, Transcription termination

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription termination factor RhoUniRule annotation (EC:3.6.4.-UniRule annotation)
Alternative name(s):
ATP-dependent helicase RhoUniRule annotation
Gene namesi
Name:rhoUniRule annotation
Ordered Locus Names:SF3856, S3903
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Transcription termination factor RhoPRO_0000188978Add
BLAST

Proteomic databases

PaxDbiP0AG33.
PRIDEiP0AG33.

Interactioni

Subunit structurei

Homohexamer. The homohexamer assembles into an open ring structure.UniRule annotation

Protein-protein interaction databases

STRINGi198214.SF3856.

Structurei

3D structure databases

ProteinModelPortaliP0AG33.
SMRiP0AG33. Positions 1-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni61 – 666RNA-binding 1UniRule annotation
Regioni78 – 803RNA-binding 1UniRule annotation
Regioni108 – 1103RNA-binding 1UniRule annotation
Regioni284 – 2885RNA-binding 2UniRule annotation

Sequence similaritiesi

Belongs to the Rho family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1158.
HOGENOMiHOG000076952.
KOiK03628.
OrthoDBiEOG6N681W.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01884. Rho.
InterProiIPR003593. AAA+_ATPase.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR011129. Cold_shock_prot.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR011112. Rho_N.
IPR011113. Rho_RNA-bd.
IPR004665. Term_rho.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF07498. Rho_N. 1 hit.
PF07497. Rho_RNA_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00357. CSP. 1 hit.
SM00959. Rho_N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF68912. SSF68912. 1 hit.
TIGRFAMsiTIGR00767. rho. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG33-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNLTELKNTP VSELITLGEN MGLENLARMR KQDIIFAILK QHAKSGEDIF
60 70 80 90 100
GDGVLEILQD GFGFLRSADS SYLAGPDDIY VSPSQIRRFN LRTGDTISGK
110 120 130 140 150
IRPPKEGERY FALLKVNEVN FDKPENARNK ILFENLTPLH ANSRLRMERG
160 170 180 190 200
NGSTEDLTAR VLDLASPIGR GQRGLIVAPP KAGKTMLLQN IAQSIAYNHP
210 220 230 240 250
DCVLMVLLID ERPEEVTEMQ RLVKGEVVAS TFDEPASRHV QVAEMVIEKA
260 270 280 290 300
KRLVEHKKDV IILLDSITRL ARAYNTVVPA SGKVLTGGVD ANALHRPKRF
310 320 330 340 350
FGAARNVEEG GSLTIIATAL IDTGSKMDEV IYEEFKGTGN MELHLSRKIA
360 370 380 390 400
EKRVFPAIDY NRSGTRKEEL LTTQEELQKM WILRKIIHPM GEIDAMEFLI
410
NKLAMTKTND DFFEMMKRS
Length:419
Mass (Da):47,004
Last modified:July 21, 1986 - v1
Checksum:i5970A85334C43467
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN45293.2.
AE014073 Genomic DNA. Translation: AAP18904.1.
RefSeqiNP_709586.2. NC_004337.2.
NP_839093.1. NC_004741.1.

Genome annotation databases

EnsemblBacteriaiAAN45293; AAN45293; SF3856.
AAP18904; AAP18904; S3903.
GeneIDi1025988.
1076538.
KEGGisfl:SF3856.
sfx:S3903.
PATRICi18709682. VBIShiFle31049_4256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN45293.2 .
AE014073 Genomic DNA. Translation: AAP18904.1 .
RefSeqi NP_709586.2. NC_004337.2.
NP_839093.1. NC_004741.1.

3D structure databases

ProteinModelPortali P0AG33.
SMRi P0AG33. Positions 1-415.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198214.SF3856.

Proteomic databases

PaxDbi P0AG33.
PRIDEi P0AG33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN45293 ; AAN45293 ; SF3856 .
AAP18904 ; AAP18904 ; S3903 .
GeneIDi 1025988.
1076538.
KEGGi sfl:SF3856.
sfx:S3903.
PATRICi 18709682. VBIShiFle31049_4256.

Phylogenomic databases

eggNOGi COG1158.
HOGENOMi HOG000076952.
KOi K03628.
OrthoDBi EOG6N681W.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_01884. Rho.
InterProi IPR003593. AAA+_ATPase.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR011129. Cold_shock_prot.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR011112. Rho_N.
IPR011113. Rho_RNA-bd.
IPR004665. Term_rho.
[Graphical view ]
Pfami PF00006. ATP-synt_ab. 1 hit.
PF07498. Rho_N. 1 hit.
PF07497. Rho_RNA_bind. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
SM00357. CSP. 1 hit.
SM00959. Rho_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF68912. SSF68912. 1 hit.
TIGRFAMsi TIGR00767. rho. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiRHO_SHIFL
AccessioniPrimary (citable) accession number: P0AG33
Secondary accession number(s): P03002, Q48357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3