P0AG33 (RHO_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription termination factor Rho
EC=3.6.4.-

Alternative name(s):
ATP-dependent helicase Rho

Gene names

Name:	rho
Ordered Locus Names:	SF3856, S3903

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template By similarity. HAMAP-Rule MF_01884
Subunit structure	Homohexamer. The homohexamer assembles into an open ring structure By similarity.
Sequence similarities	Belongs to the Rho family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Transcription termination
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, termination Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: HAMAP RNA-dependent ATPase activity Inferred from electronic annotation. Source: InterPro helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Transcription termination factor Rho HAMAP-Rule MF_01884		PRO_0000188978
Regions
	Nucleotide binding	169 – 174	6	ATP Potential
	Nucleotide binding	181 – 186	6	ATP By similarity
	Region	61 – 66	6	RNA-binding 1 By similarity
	Region	78 – 80	3	RNA-binding 1 By similarity
	Region	108 – 110	3	RNA-binding 1 By similarity
	Region	284 – 288	5	RNA-binding 2 By similarity
Sites
	Binding site	212	1	ATP By similarity
	Site	326	1	RNA-binding 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AG33 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 5970A85334C43467	FASTA	419	47,004
	10 20 30 40 50 60 MNLTELKNTP VSELITLGEN MGLENLARMR KQDIIFAILK QHAKSGEDIF GDGVLEILQD 70 80 90 100 110 120 GFGFLRSADS SYLAGPDDIY VSPSQIRRFN LRTGDTISGK IRPPKEGERY FALLKVNEVN 130 140 150 160 170 180 FDKPENARNK ILFENLTPLH ANSRLRMERG NGSTEDLTAR VLDLASPIGR GQRGLIVAPP 190 200 210 220 230 240 KAGKTMLLQN IAQSIAYNHP DCVLMVLLID ERPEEVTEMQ RLVKGEVVAS TFDEPASRHV 250 260 270 280 290 300 QVAEMVIEKA KRLVEHKKDV IILLDSITRL ARAYNTVVPA SGKVLTGGVD ANALHRPKRF 310 320 330 340 350 360 FGAARNVEEG GSLTIIATAL IDTGSKMDEV IYEEFKGTGN MELHLSRKIA EKRVFPAIDY 370 380 390 400 410 NRSGTRKEEL LTTQEELQKM WILRKIIHPM GEIDAMEFLI NKLAMTKTND DFFEMMKRS

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN45293.2. AE014073 Genomic DNA. Translation: AAP18904.1.
RefSeq	NP_709586.2. NC_004337.2. NP_839093.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0AG33.
SMR	P0AG33. Positions 1-415.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF3856.
Proteomic databases
PaxDb	P0AG33.
PRIDE	P0AG33.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN45293; AAN45293; SF3856. AAP18904; AAP18904; S3903.
GeneID	1025988. 1076538.
KEGG	sfl:SF3856. sfx:S3903.
PATRIC	18709682. VBIShiFle31049_4256.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1158.
HOGENOM	HOG000076952.
KO	K03628.
ProtClustDB	PRK09376.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_01884. Rho.
InterPro	IPR003593. AAA+_ATPase. IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd. IPR011129. Cold_shock_prot. IPR012340. NA-bd_OB-fold. IPR027417. P-loop_NTPase. IPR011112. Rho_N. IPR011113. Rho_RNA-bd. IPR004665. Term_rho. [Graphical view]
Pfam	PF00006. ATP-synt_ab. 1 hit. PF07498. Rho_N. 1 hit. PF07497. Rho_RNA_bind. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00357. CSP. 1 hit. SM00959. Rho_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF68912. Rho_N. 1 hit. SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00767. rho. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RHO_SHIFL

Accession

Primary (citable) accession number: P0AG33
Secondary accession number(s): P03002, Q48357

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 29, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program