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P0AG33 (RHO_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription termination factor Rho

EC=3.6.4.-
Alternative name(s):
ATP-dependent helicase Rho
Gene names
Name:rho
Ordered Locus Names:SF3856, S3903
OrganismShigella flexneri [Complete proteome] [HAMAP]
Taxonomic identifier623 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template By similarity. HAMAP-Rule MF_01884

Subunit structure

Homohexamer. The homohexamer assembles into an open ring structure By similarity.

Sequence similarities

Belongs to the Rho family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419Transcription termination factor Rho HAMAP-Rule MF_01884
PRO_0000188978

Regions

Nucleotide binding169 – 1746ATP Potential
Nucleotide binding181 – 1866ATP By similarity
Region61 – 666RNA-binding 1 By similarity
Region78 – 803RNA-binding 1 By similarity
Region108 – 1103RNA-binding 1 By similarity
Region284 – 2885RNA-binding 2 By similarity

Sites

Binding site2121ATP By similarity
Site3261RNA-binding 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0AG33 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 5970A85334C43467

FASTA41947,004
        10         20         30         40         50         60 
MNLTELKNTP VSELITLGEN MGLENLARMR KQDIIFAILK QHAKSGEDIF GDGVLEILQD 

        70         80         90        100        110        120 
GFGFLRSADS SYLAGPDDIY VSPSQIRRFN LRTGDTISGK IRPPKEGERY FALLKVNEVN 

       130        140        150        160        170        180 
FDKPENARNK ILFENLTPLH ANSRLRMERG NGSTEDLTAR VLDLASPIGR GQRGLIVAPP 

       190        200        210        220        230        240 
KAGKTMLLQN IAQSIAYNHP DCVLMVLLID ERPEEVTEMQ RLVKGEVVAS TFDEPASRHV 

       250        260        270        280        290        300 
QVAEMVIEKA KRLVEHKKDV IILLDSITRL ARAYNTVVPA SGKVLTGGVD ANALHRPKRF 

       310        320        330        340        350        360 
FGAARNVEEG GSLTIIATAL IDTGSKMDEV IYEEFKGTGN MELHLSRKIA EKRVFPAIDY 

       370        380        390        400        410 
NRSGTRKEEL LTTQEELQKM WILRKIIHPM GEIDAMEFLI NKLAMTKTND DFFEMMKRS 

« Hide

References

[1]"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. expand/collapse author list , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 301 / Serotype 2a.
[2]"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T."
Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.
Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005674 Genomic DNA. Translation: AAN45293.2.
AE014073 Genomic DNA. Translation: AAP18904.1.
RefSeqNP_709586.2. NC_004337.2.
NP_839093.1. NC_004741.1.

3D structure databases

ProteinModelPortalP0AG33.
SMRP0AG33. Positions 1-415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198214.SF3856.

Proteomic databases

PaxDbP0AG33.
PRIDEP0AG33.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN45293; AAN45293; SF3856.
AAP18904; AAP18904; S3903.
GeneID1025988.
1076538.
KEGGsfl:SF3856.
sfx:S3903.
PATRIC18709682. VBIShiFle31049_4256.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1158.
HOGENOMHOG000076952.
KOK03628.
OrthoDBEOG6N681W.
ProtClustDBPRK09376.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_01884. Rho.
InterProIPR003593. AAA+_ATPase.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR011129. Cold_shock_prot.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR011112. Rho_N.
IPR011113. Rho_RNA-bd.
IPR004665. Term_rho.
[Graphical view]
PfamPF00006. ATP-synt_ab. 1 hit.
PF07498. Rho_N. 1 hit.
PF07497. Rho_RNA_bind. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00357. CSP. 1 hit.
SM00959. Rho_N. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF68912. SSF68912. 1 hit.
TIGRFAMsTIGR00767. rho. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRHO_SHIFL
AccessionPrimary (citable) accession number: P0AG33
Secondary accession number(s): P03002, Q48357
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families