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P0AG33

- RHO_SHIFL

UniProt

P0AG33 - RHO_SHIFL

Protein

Transcription termination factor Rho

Gene

rho

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei212 – 2121ATPUniRule annotation
    Sitei326 – 3261RNA-binding 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi169 – 1746ATPUniRule annotation
    Nucleotide bindingi181 – 1866ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. helicase activity Source: UniProtKB-HAMAP
    3. RNA binding Source: UniProtKB-HAMAP
    4. RNA-dependent ATPase activity Source: InterPro

    GO - Biological processi

    1. DNA-templated transcription, termination Source: UniProtKB-HAMAP
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Transcription, Transcription regulation, Transcription termination

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription termination factor RhoUniRule annotation (EC:3.6.4.-UniRule annotation)
    Alternative name(s):
    ATP-dependent helicase RhoUniRule annotation
    Gene namesi
    Name:rhoUniRule annotation
    Ordered Locus Names:SF3856, S3903
    OrganismiShigella flexneri
    Taxonomic identifieri623 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 419419Transcription termination factor RhoPRO_0000188978Add
    BLAST

    Proteomic databases

    PaxDbiP0AG33.
    PRIDEiP0AG33.

    Interactioni

    Subunit structurei

    Homohexamer. The homohexamer assembles into an open ring structure.UniRule annotation

    Protein-protein interaction databases

    STRINGi198214.SF3856.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AG33.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni61 – 666RNA-binding 1UniRule annotation
    Regioni78 – 803RNA-binding 1UniRule annotation
    Regioni108 – 1103RNA-binding 1UniRule annotation
    Regioni284 – 2885RNA-binding 2UniRule annotation

    Sequence similaritiesi

    Belongs to the Rho family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1158.
    HOGENOMiHOG000076952.
    KOiK03628.
    OrthoDBiEOG6N681W.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_01884. Rho.
    InterProiIPR003593. AAA+_ATPase.
    IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
    IPR011129. Cold_shock_prot.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    IPR011112. Rho_N.
    IPR011113. Rho_RNA-bd.
    IPR004665. Term_rho.
    [Graphical view]
    PfamiPF00006. ATP-synt_ab. 1 hit.
    PF07498. Rho_N. 1 hit.
    PF07497. Rho_RNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    SM00357. CSP. 1 hit.
    SM00959. Rho_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF68912. SSF68912. 1 hit.
    TIGRFAMsiTIGR00767. rho. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AG33-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLTELKNTP VSELITLGEN MGLENLARMR KQDIIFAILK QHAKSGEDIF    50
    GDGVLEILQD GFGFLRSADS SYLAGPDDIY VSPSQIRRFN LRTGDTISGK 100
    IRPPKEGERY FALLKVNEVN FDKPENARNK ILFENLTPLH ANSRLRMERG 150
    NGSTEDLTAR VLDLASPIGR GQRGLIVAPP KAGKTMLLQN IAQSIAYNHP 200
    DCVLMVLLID ERPEEVTEMQ RLVKGEVVAS TFDEPASRHV QVAEMVIEKA 250
    KRLVEHKKDV IILLDSITRL ARAYNTVVPA SGKVLTGGVD ANALHRPKRF 300
    FGAARNVEEG GSLTIIATAL IDTGSKMDEV IYEEFKGTGN MELHLSRKIA 350
    EKRVFPAIDY NRSGTRKEEL LTTQEELQKM WILRKIIHPM GEIDAMEFLI 400
    NKLAMTKTND DFFEMMKRS 419
    Length:419
    Mass (Da):47,004
    Last modified:July 21, 1986 - v1
    Checksum:i5970A85334C43467
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN45293.2.
    AE014073 Genomic DNA. Translation: AAP18904.1.
    RefSeqiNP_709586.2. NC_004337.2.
    NP_839093.1. NC_004741.1.

    Genome annotation databases

    EnsemblBacteriaiAAN45293; AAN45293; SF3856.
    AAP18904; AAP18904; S3903.
    GeneIDi1025988.
    1076538.
    KEGGisfl:SF3856.
    sfx:S3903.
    PATRICi18709682. VBIShiFle31049_4256.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN45293.2 .
    AE014073 Genomic DNA. Translation: AAP18904.1 .
    RefSeqi NP_709586.2. NC_004337.2.
    NP_839093.1. NC_004741.1.

    3D structure databases

    ProteinModelPortali P0AG33.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198214.SF3856.

    Proteomic databases

    PaxDbi P0AG33.
    PRIDEi P0AG33.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN45293 ; AAN45293 ; SF3856 .
    AAP18904 ; AAP18904 ; S3903 .
    GeneIDi 1025988.
    1076538.
    KEGGi sfl:SF3856.
    sfx:S3903.
    PATRICi 18709682. VBIShiFle31049_4256.

    Phylogenomic databases

    eggNOGi COG1158.
    HOGENOMi HOG000076952.
    KOi K03628.
    OrthoDBi EOG6N681W.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_01884. Rho.
    InterProi IPR003593. AAA+_ATPase.
    IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
    IPR011129. Cold_shock_prot.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    IPR011112. Rho_N.
    IPR011113. Rho_RNA-bd.
    IPR004665. Term_rho.
    [Graphical view ]
    Pfami PF00006. ATP-synt_ab. 1 hit.
    PF07498. Rho_N. 1 hit.
    PF07497. Rho_RNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    SM00357. CSP. 1 hit.
    SM00959. Rho_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF68912. SSF68912. 1 hit.
    TIGRFAMsi TIGR00767. rho. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
      Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
      , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
      Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 301 / Serotype 2a.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700930 / 2457T / Serotype 2a.

    Entry informationi

    Entry nameiRHO_SHIFL
    AccessioniPrimary (citable) accession number: P0AG33
    Secondary accession number(s): P03002, Q48357
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3