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Protein

Transcription termination factor Rho

Gene

rho

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei212ATPUniRule annotation1
Sitei326RNA-binding 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi169 – 174ATPUniRule annotation6
Nucleotide bindingi181 – 186ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation, Transcription termination

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciECOO157:RHO-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription termination factor RhoUniRule annotation (EC:3.6.4.-UniRule annotation)
Alternative name(s):
ATP-dependent helicase RhoUniRule annotation
Gene namesi
Name:rhoUniRule annotation
Ordered Locus Names:Z5293, ECs4716
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001889631 – 419Transcription termination factor RhoAdd BLAST419

Proteomic databases

PRIDEiP0AG32.

Interactioni

Subunit structurei

Homohexamer. The homohexamer assembles into an open ring structure.UniRule annotation

Protein-protein interaction databases

MINTiMINT-1222519.
STRINGi155864.Z5293.

Structurei

3D structure databases

ProteinModelPortaliP0AG32.
SMRiP0AG32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni61 – 66RNA-binding 1UniRule annotation6
Regioni78 – 80RNA-binding 1UniRule annotation3
Regioni108 – 110RNA-binding 1UniRule annotation3
Regioni284 – 288RNA-binding 2UniRule annotation5

Sequence similaritiesi

Belongs to the Rho family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1158. LUCA.
HOGENOMiHOG000076952.
KOiK03628.
OMAiFLRAPDY.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01884. Rho. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR011129. Cold_shock_prot.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR011112. Rho_N.
IPR011113. Rho_RNA-bd.
IPR004665. Term_rho.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF07498. Rho_N. 1 hit.
PF07497. Rho_RNA_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00357. CSP. 1 hit.
SM00959. Rho_N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF68912. SSF68912. 1 hit.
TIGRFAMsiTIGR00767. rho. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLTELKNTP VSELITLGEN MGLENLARMR KQDIIFAILK QHAKSGEDIF
60 70 80 90 100
GDGVLEILQD GFGFLRSADS SYLAGPDDIY VSPSQIRRFN LRTGDTISGK
110 120 130 140 150
IRPPKEGERY FALLKVNEVN FDKPENARNK ILFENLTPLH ANSRLRMERG
160 170 180 190 200
NGSTEDLTAR VLDLASPIGR GQRGLIVAPP KAGKTMLLQN IAQSIAYNHP
210 220 230 240 250
DCVLMVLLID ERPEEVTEMQ RLVKGEVVAS TFDEPASRHV QVAEMVIEKA
260 270 280 290 300
KRLVEHKKDV IILLDSITRL ARAYNTVVPA SGKVLTGGVD ANALHRPKRF
310 320 330 340 350
FGAARNVEEG GSLTIIATAL IDTGSKMDEV IYEEFKGTGN MELHLSRKIA
360 370 380 390 400
EKRVFPAIDY NRSGTRKEEL LTTQEELQKM WILRKIIHPM GEIDAMEFLI
410
NKLAMTKTND DFFEMMKRS
Length:419
Mass (Da):47,004
Last modified:July 21, 1986 - v1
Checksum:i5970A85334C43467
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58977.1.
BA000007 Genomic DNA. Translation: BAB38139.1.
PIRiD91218.
RefSeqiNP_312743.1. NC_002695.1.
WP_001054527.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG58977; AAG58977; Z5293.
BAB38139; BAB38139; BAB38139.
GeneIDi915249.
KEGGiece:Z5293.
ecs:ECs4716.
PATRICi18359037. VBIEscCol44059_4677.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58977.1.
BA000007 Genomic DNA. Translation: BAB38139.1.
PIRiD91218.
RefSeqiNP_312743.1. NC_002695.1.
WP_001054527.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP0AG32.
SMRiP0AG32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1222519.
STRINGi155864.Z5293.

Proteomic databases

PRIDEiP0AG32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58977; AAG58977; Z5293.
BAB38139; BAB38139; BAB38139.
GeneIDi915249.
KEGGiece:Z5293.
ecs:ECs4716.
PATRICi18359037. VBIEscCol44059_4677.

Phylogenomic databases

eggNOGiCOG1158. LUCA.
HOGENOMiHOG000076952.
KOiK03628.
OMAiFLRAPDY.

Enzyme and pathway databases

BioCyciECOO157:RHO-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01884. Rho. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR011129. Cold_shock_prot.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR011112. Rho_N.
IPR011113. Rho_RNA-bd.
IPR004665. Term_rho.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF07498. Rho_N. 1 hit.
PF07497. Rho_RNA_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00357. CSP. 1 hit.
SM00959. Rho_N. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF68912. SSF68912. 1 hit.
TIGRFAMsiTIGR00767. rho. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRHO_ECO57
AccessioniPrimary (citable) accession number: P0AG32
Secondary accession number(s): P03002, Q48357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.