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P0AG26

- SPOT_SHIFL

UniProt

P0AG26 - SPOT_SHIFL

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Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene
spoT, SF3690, S4079
Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp By similarity.

Catalytic activityi

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactori

Manganese By similarity.

Pathwayi

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity Source: UniProtKB-EC
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: InterPro

GO - Biological processi

  1. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

UniPathwayiUPA00908; UER00886.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
Gene namesi
Name:spoT
Ordered Locus Names:SF3690, S4079
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 702702Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolasePRO_0000166574Add
BLAST

Proteomic databases

PRIDEiP0AG26.

Interactioni

Protein-protein interaction databases

STRINGi198214.SF3690.

Structurei

3D structure databases

ProteinModelPortaliP0AG26.
SMRiP0AG26. Positions 5-339, 387-448.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 144100HDAdd
BLAST
Domaini628 – 70275ACTAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.
Contains 1 ACT domain.
Contains 1 HD domain.

Phylogenomic databases

eggNOGiCOG0317.
HOGENOMiHOG000018299.
KOiK01139.
OMAiAMHPMRS.
OrthoDBiEOG6SV551.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AG26-1 [UniParc]FASTAAdd to Basket

« Hide

MYLFESLNQL IQTYLPEDQI KRLRQAYLVA RDAHEGQTRS SGEPYITHPV    50
AVACILAEMK LDYETLMAAL LHDVIEDTPA TYQDMEQLFG KSVAELVEGV 100
SKLDKLKFRD KKEAQAENFR KMIMAMVQDI RVILIKLADR THNMRTLGSL 150
RPDKRRRIAR ETLEIYSPLA HRLGIHHIKT ELEELGFEAL YPNRYRVIKE 200
VVKAARGNRK EMIQKILSEI EGRLQEAGIP CRVSGREKHL YSIYCKMVLK 250
EQRFHSIMDI YAFRVIVNDS DTCYRVLGQM HSLYKPRPGR VKDYIAIPKA 300
NGYQSLHTSM IGPHGVPVEV QIRTEDMDQM AEMGVAAHWA YKEHGETSTT 350
AQIRAQRWMQ SLLELQQSAG SSFEFIESVK SDLFPDEIYV FTPEGRIVEL 400
PAGATPVDFA YAVHTDIGHA CVGARVDRQP YPLSQPLTSG QTVEIITAPG 450
ARPNAAWLNF VVSSKARAKI RQLLKNLKRD DSVSLGRRLL NHALGGSRKL 500
NEIPQENIQR ELDRMKLATL DDLLAEIGLG NAMSVVVAKN LQHGDASIPP 550
ATQSHGHLPI KGADGVLITF AKCCRPIPGD PIIAHVSPGK GLVIHHESCR 600
NIRGYQKEPE KFMAVEWDKE TAQEFITEIK VEMFNHQGAL ANLTAAINTT 650
TSNIQSLNTE EKDGRVYSAF IRLTARDRVH LANIMRKIRV MPDVIKVTRN 700
RN 702
Length:702
Mass (Da):79,342
Last modified:December 20, 2005 - v1
Checksum:iA85F70F57D082EE8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN45137.1.
AE014073 Genomic DNA. Translation: AAP19056.1.
RefSeqiNP_709430.1. NC_004337.2.
NP_839245.1. NC_004741.1.

Genome annotation databases

EnsemblBacteriaiAAN45137; AAN45137; SF3690.
AAP19056; AAP19056; S4079.
GeneIDi1026895.
1080286.
KEGGisfl:SF3690.
sfx:S4079.
PATRICi18710066. VBIShiFle31049_4440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN45137.1 .
AE014073 Genomic DNA. Translation: AAP19056.1 .
RefSeqi NP_709430.1. NC_004337.2.
NP_839245.1. NC_004741.1.

3D structure databases

ProteinModelPortali P0AG26.
SMRi P0AG26. Positions 5-339, 387-448.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198214.SF3690.

Proteomic databases

PRIDEi P0AG26.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN45137 ; AAN45137 ; SF3690 .
AAP19056 ; AAP19056 ; S4079 .
GeneIDi 1026895.
1080286.
KEGGi sfl:SF3690.
sfx:S4079.
PATRICi 18710066. VBIShiFle31049_4440.

Phylogenomic databases

eggNOGi COG0317.
HOGENOMi HOG000018299.
KOi K01139.
OMAi AMHPMRS.
OrthoDBi EOG6SV551.

Enzyme and pathway databases

UniPathwayi UPA00908 ; UER00886 .

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view ]
Pfami PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view ]
SUPFAMi SSF81271. SSF81271. 1 hit.
TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
PROSITEi PS51671. ACT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiSPOT_SHIFL
AccessioniPrimary (citable) accession number: P0AG26
Secondary accession number(s): P17580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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