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Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp (By similarity).By similarity

Catalytic activityi

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactori

Mn2+By similarity

Pathwayi: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GDP.
Proteins known to be involved in this subpathway in this organism are:
  1. Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT)
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GDP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandManganese

Enzyme and pathway databases

UniPathwayiUPA00908; UER00886

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
Gene namesi
Name:spoT
Ordered Locus Names:SF3690, S4079
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001665741 – 702Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolaseAdd BLAST702

Structurei

3D structure databases

ProteinModelPortaliP0AG26
SMRiP0AG26
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 144HDPROSITE-ProRule annotationAdd BLAST100
Domaini628 – 702ACTPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated

Phylogenomic databases

eggNOGiENOG4105CWR Bacteria
COG0317 LUCA
HOGENOMiHOG000018299
KOiK01139
OMAiHYYSIYQ

Family and domain databases

CDDicd00077 HDc, 1 hit
cd05399 NT_Rel-Spo_like, 1 hit
cd01668 TGS_RelA_SpoT, 1 hit
Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR012675 Beta-grasp_dom_sf
IPR003607 HD/PDEase_dom
IPR004811 RelA/Spo_fam
IPR007685 RelA_SpoT
IPR004095 TGS
IPR012676 TGS-like
IPR033655 TGS_RelA
PfamiView protein in Pfam
PF13291 ACT_4, 1 hit
PF13328 HD_4, 1 hit
PF04607 RelA_SpoT, 1 hit
PF02824 TGS, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
SM00954 RelA_SpoT, 1 hit
SUPFAMiSSF81271 SSF81271, 1 hit
TIGRFAMsiTIGR00691 spoT_relA, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

P0AG26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYLFESLNQL IQTYLPEDQI KRLRQAYLVA RDAHEGQTRS SGEPYITHPV
60 70 80 90 100
AVACILAEMK LDYETLMAAL LHDVIEDTPA TYQDMEQLFG KSVAELVEGV
110 120 130 140 150
SKLDKLKFRD KKEAQAENFR KMIMAMVQDI RVILIKLADR THNMRTLGSL
160 170 180 190 200
RPDKRRRIAR ETLEIYSPLA HRLGIHHIKT ELEELGFEAL YPNRYRVIKE
210 220 230 240 250
VVKAARGNRK EMIQKILSEI EGRLQEAGIP CRVSGREKHL YSIYCKMVLK
260 270 280 290 300
EQRFHSIMDI YAFRVIVNDS DTCYRVLGQM HSLYKPRPGR VKDYIAIPKA
310 320 330 340 350
NGYQSLHTSM IGPHGVPVEV QIRTEDMDQM AEMGVAAHWA YKEHGETSTT
360 370 380 390 400
AQIRAQRWMQ SLLELQQSAG SSFEFIESVK SDLFPDEIYV FTPEGRIVEL
410 420 430 440 450
PAGATPVDFA YAVHTDIGHA CVGARVDRQP YPLSQPLTSG QTVEIITAPG
460 470 480 490 500
ARPNAAWLNF VVSSKARAKI RQLLKNLKRD DSVSLGRRLL NHALGGSRKL
510 520 530 540 550
NEIPQENIQR ELDRMKLATL DDLLAEIGLG NAMSVVVAKN LQHGDASIPP
560 570 580 590 600
ATQSHGHLPI KGADGVLITF AKCCRPIPGD PIIAHVSPGK GLVIHHESCR
610 620 630 640 650
NIRGYQKEPE KFMAVEWDKE TAQEFITEIK VEMFNHQGAL ANLTAAINTT
660 670 680 690 700
TSNIQSLNTE EKDGRVYSAF IRLTARDRVH LANIMRKIRV MPDVIKVTRN

RN
Length:702
Mass (Da):79,342
Last modified:December 20, 2005 - v1
Checksum:iA85F70F57D082EE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA Translation: AAN45137.1
AE014073 Genomic DNA Translation: AAP19056.1
RefSeqiNP_709430.1, NC_004337.2
WP_000280488.1, NZ_NIYV01000025.1

Genome annotation databases

EnsemblBacteriaiAAN45137; AAN45137; SF3690
AAP19056; AAP19056; S4079
GeneIDi1026895
KEGGisfl:SF3690
sfx:S4079
PATRICifig|198214.7.peg.4354

Similar proteinsi

Entry informationi

Entry nameiSPOT_SHIFL
AccessioniPrimary (citable) accession number: P0AG26
Secondary accession number(s): P17580
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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