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P0AG25

- SPOT_ECO57

UniProt

P0AG25 - SPOT_ECO57

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Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp (By similarity).By similarity

Catalytic activityi

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactori

Manganese.By similarity

Pathwayi

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

BioCyciECOL386585:GJFA-4493-MONOMER.
ECOO157:SPOT-MONOMER.
UniPathwayiUPA00908; UER00886.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
Gene namesi
Name:spoT
Ordered Locus Names:Z5076, ECs4525
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 702702Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolasePRO_0000166570Add
BLAST

Proteomic databases

PRIDEiP0AG25.

Interactioni

Protein-protein interaction databases

MINTiMINT-1219842.
STRINGi155864.Z5076.

Structurei

3D structure databases

ProteinModelPortaliP0AG25.
SMRiP0AG25. Positions 5-339, 387-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 144100HDAdd
BLAST
Domaini628 – 70275ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiCOG0317.
HOGENOMiHOG000018299.
KOiK01139.
OMAiIGYITRG.
OrthoDBiEOG6SV551.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AG25-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYLFESLNQL IQTYLPEDQI KRLRQAYLVA RDAHEGQTRS SGEPYITHPV
60 70 80 90 100
AVACILAEMK LDYETLMAAL LHDVIEDTPA TYQDMEQLFG KSVAELVEGV
110 120 130 140 150
SKLDKLKFRD KKEAQAENFR KMIMAMVQDI RVILIKLADR THNMRTLGSL
160 170 180 190 200
RPDKRRRIAR ETLEIYSPLA HRLGIHHIKT ELEELGFEAL YPNRYRVIKE
210 220 230 240 250
VVKAARGNRK EMIQKILSEI EGRLQEAGIP CRVSGREKHL YSIYCKMVLK
260 270 280 290 300
EQRFHSIMDI YAFRVIVNDS DTCYRVLGQM HSLYKPRPGR VKDYIAIPKA
310 320 330 340 350
NGYQSLHTSM IGPHGVPVEV QIRTEDMDQM AEMGVAAHWA YKEHGETSTT
360 370 380 390 400
AQIRAQRWMQ SLLELQQSAG SSFEFIESVK SDLFPDEIYV FTPEGRIVEL
410 420 430 440 450
PAGATPVDFA YAVHTDIGHA CVGARVDRQP YPLSQPLTSG QTVEIITAPG
460 470 480 490 500
ARPNAAWLNF VVSSKARAKI RQLLKNLKRD DSVSLGRRLL NHALGGSRKL
510 520 530 540 550
NEIPQENIQR ELDRMKLATL DDLLAEIGLG NAMSVVVAKN LQHGDASIPP
560 570 580 590 600
ATQSHGHLPI KGADGVLITF AKCCRPIPGD PIIAHVSPGK GLVIHHESCR
610 620 630 640 650
NIRGYQKEPE KFMAVEWDKE TAQEFITEIK VEMFNHQGAL ANLTAAINTT
660 670 680 690 700
TSNIQSLNTE EKDGRVYSAF IRLTARDRVH LANIMRKIRV MPDVIKVTRN

RN
Length:702
Mass (Da):79,342
Last modified:December 20, 2005 - v1
Checksum:iA85F70F57D082EE8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG58794.1.
BA000007 Genomic DNA. Translation: BAB37948.1.
PIRiE91194.
F86041.
RefSeqiNP_290230.1. NC_002655.2.
NP_312552.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG58794; AAG58794; Z5076.
BAB37948; BAB37948; BAB37948.
GeneIDi915518.
960895.
KEGGiece:Z5076.
ecs:ECs4525.
PATRICi18358673. VBIEscCol44059_4502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG58794.1 .
BA000007 Genomic DNA. Translation: BAB37948.1 .
PIRi E91194.
F86041.
RefSeqi NP_290230.1. NC_002655.2.
NP_312552.1. NC_002695.1.

3D structure databases

ProteinModelPortali P0AG25.
SMRi P0AG25. Positions 5-339, 387-448.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1219842.
STRINGi 155864.Z5076.

Proteomic databases

PRIDEi P0AG25.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG58794 ; AAG58794 ; Z5076 .
BAB37948 ; BAB37948 ; BAB37948 .
GeneIDi 915518.
960895.
KEGGi ece:Z5076.
ecs:ECs4525.
PATRICi 18358673. VBIEscCol44059_4502.

Phylogenomic databases

eggNOGi COG0317.
HOGENOMi HOG000018299.
KOi K01139.
OMAi IGYITRG.
OrthoDBi EOG6SV551.

Enzyme and pathway databases

UniPathwayi UPA00908 ; UER00886 .
BioCyci ECOL386585:GJFA-4493-MONOMER.
ECOO157:SPOT-MONOMER.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view ]
Pfami PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view ]
SUPFAMi SSF81271. SSF81271. 1 hit.
TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
PROSITEi PS51671. ACT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiSPOT_ECO57
AccessioniPrimary (citable) accession number: P0AG25
Secondary accession number(s): P17580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3