##gff-version 3
P0AG24	UniProtKB	Chain	1	702	.	.	.	ID=PRO_0000166569;Note=Bifunctional (p)ppGpp synthase/hydrolase SpoT	
P0AG24	UniProtKB	Domain	45	144	.	.	.	Note=HD;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU01175	
P0AG24	UniProtKB	Domain	386	447	.	.	.	Note=TGS;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU01228	
P0AG24	UniProtKB	Domain	628	702	.	.	.	Note=ACT;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU01007	
P0AG24	UniProtKB	Mutagenesis	73	73	.	.	.	Note=Obviates hydrolysis of ppGpp%2C moderately decreases biofilm formation%2C loss of biofilm induction in response to translation inhibitors. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19460094;Dbxref=PMID:19460094	
P0AG24	UniProtKB	Mutagenesis	259	259	.	.	.	Note=Obviates synthesis of ppGpp%2C strongly increases biofilm formation. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:19460094;Dbxref=PMID:19460094	
P0AG24	UniProtKB	Mutagenesis	293	293	.	.	.	Note=Unable to restore (p)ppGpp synthesis nor complement a relA/spoT double disruption. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12596879;Dbxref=PMID:12596879	
P0AG24	UniProtKB	Mutagenesis	294	294	.	.	.	Note=Synthesizes (p)ppGpp%2C complements a relA/spoT double disruption. Y->I