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P0AG20

- RELA_ECOLI

UniProt

P0AG20 - RELA_ECOLI

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Protein

GTP pyrophosphokinase

Gene

relA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp.2 Publications

Catalytic activityi

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathwayi

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. GTP binding Source: UniProtKB-KW
  4. GTP diphosphokinase activity Source: EcoCyc
  5. kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. guanosine tetraphosphate metabolic process Source: EcoCyc
  3. nucleobase-containing small molecule interconversion Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:RELA-MONOMER.
ECOL316407:JW2755-MONOMER.
MetaCyc:RELA-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP pyrophosphokinase (EC:2.7.6.5)
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene namesi
Name:relA
Ordered Locus Names:b2784, JW2755
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10835. relA.

Pathology & Biotechi

Disruption phenotypei

A slight increase in biofilm formation in a csrA-disrupted background; when combined with a spoT disruption (a ppGpp0 mutant) there is a very large increase in biofilm formation. Deletion of relA alone decreases persister cell formation in a hipA7 mutant; the double relA/spoT deletion obviates persister cell formation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744GTP pyrophosphokinasePRO_0000166546Add
BLAST

Proteomic databases

PaxDbiP0AG20.
PRIDEiP0AG20.

Expressioni

Gene expression databases

GenevestigatoriP0AG20.

Interactioni

Protein-protein interaction databases

DIPiDIP-10658N.
IntActiP0AG20. 15 interactions.
STRINGi511145.b2784.

Structurei

3D structure databases

ProteinModelPortaliP0AG20.
SMRiP0AG20. Positions 60-355, 406-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini668 – 74376ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0317.
HOGENOMiHOG000018300.
InParanoidiP0AG20.
KOiK00951.
OMAiTEIGHNC.
OrthoDBiEOG6SV551.
PhylomeDBiP0AG20.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AG20-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVAVRSAHIN KAGEFDPEKW IASLGITSQK SCECLAETWA YCLQQTQGHP
60 70 80 90 100
DASLLLWRGV EMVEILSTLS MDIDTLRAAL LFPLADANVV SEDVLRESVG
110 120 130 140 150
KSVVNLIHGV RDMAAIRQLK ATHTDSVSSE QVDNVRRMLL AMVDDFRCVV
160 170 180 190 200
IKLAERIAHL REVKDAPEDE RVLAAKECTN IYAPLANRLG IGQLKWELED
210 220 230 240 250
YCFRYLHPTE YKRIAKLLHE RRLDREHYIE EFVGHLRAEM KAEGVKAEVY
260 270 280 290 300
GRPKHIYSIW RKMQKKNLAF DELFDVRAVR IVAERLQDCY AALGIVHTHY
310 320 330 340 350
RHLPDEFDDY VANPKPNGYQ SIHTVVLGPG GKTVEIQIRT KQMHEDAELG
360 370 380 390 400
VAAHWKYKEG AAAGGARSGH EDRIAWLRKL IAWQEEMADS GEMLDEVRSQ
410 420 430 440 450
VFDDRVYVFT PKGDVVDLPA GSTPLDFAYH IHSDVGHRCI GAKIGGRIVP
460 470 480 490 500
FTYQLQMGDQ IEIITQKQPN PSRDWLNPNL GYVTTSRGRS KIHAWFRKQD
510 520 530 540 550
RDKNILAGRQ ILDDELEHLG ISLKEAEKHL LPRYNFNDVD ELLAAIGGGD
560 570 580 590 600
IRLNQMVNFL QSQFNKPSAE EQDAAALKQL QQKSYTPQNR SKDNGRVVVE
610 620 630 640 650
GVGNLMHHIA RCCQPIPGDE IVGFITQGRG ISVHRADCEQ LAELRSHAPE
660 670 680 690 700
RIVDAVWGES YSAGYSLVVR VVANDRSGLL RDITTILANE KVNVLGVASR
710 720 730 740
SDTKQQLATI DMTIEIYNLQ VLGRVLGKLN QVPDVIDARR LHGS
Length:744
Mass (Da):83,876
Last modified:December 20, 2005 - v1
Checksum:iFA269709F15E1F25
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti307 – 3071F → K in AAA03237. (PubMed:2844820)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04039 Unassigned DNA. Translation: AAA03237.1.
U29580 Genomic DNA. Translation: AAA69294.1.
U00096 Genomic DNA. Translation: AAC75826.1.
AP009048 Genomic DNA. Translation: BAE76858.1.
PIRiD65060. KIECG.
RefSeqiNP_417264.1. NC_000913.3.
YP_490992.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75826; AAC75826; b2784.
BAE76858; BAE76858; BAE76858.
GeneIDi12930232.
947244.
KEGGiecj:Y75_p2721.
eco:b2784.
PATRICi32120984. VBIEscCol129921_2884.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04039 Unassigned DNA. Translation: AAA03237.1 .
U29580 Genomic DNA. Translation: AAA69294.1 .
U00096 Genomic DNA. Translation: AAC75826.1 .
AP009048 Genomic DNA. Translation: BAE76858.1 .
PIRi D65060. KIECG.
RefSeqi NP_417264.1. NC_000913.3.
YP_490992.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AG20.
SMRi P0AG20. Positions 60-355, 406-465.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10658N.
IntActi P0AG20. 15 interactions.
STRINGi 511145.b2784.

Chemistry

ChEMBLi CHEMBL1163114.

Proteomic databases

PaxDbi P0AG20.
PRIDEi P0AG20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75826 ; AAC75826 ; b2784 .
BAE76858 ; BAE76858 ; BAE76858 .
GeneIDi 12930232.
947244.
KEGGi ecj:Y75_p2721.
eco:b2784.
PATRICi 32120984. VBIEscCol129921_2884.

Organism-specific databases

EchoBASEi EB0828.
EcoGenei EG10835. relA.

Phylogenomic databases

eggNOGi COG0317.
HOGENOMi HOG000018300.
InParanoidi P0AG20.
KOi K00951.
OMAi TEIGHNC.
OrthoDBi EOG6SV551.
PhylomeDBi P0AG20.

Enzyme and pathway databases

UniPathwayi UPA00908 ; UER00884 .
BioCyci EcoCyc:RELA-MONOMER.
ECOL316407:JW2755-MONOMER.
MetaCyc:RELA-MONOMER.

Miscellaneous databases

PROi P0AG20.

Gene expression databases

Genevestigatori P0AG20.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view ]
SMARTi SM00954. RelA_SpoT. 1 hit.
[Graphical view ]
SUPFAMi SSF81271. SSF81271. 1 hit.
TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
PROSITEi PS51671. ACT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence and characterization of the relA gene of Escherichia coli."
    Metzger S., Dror I.B., Aizenman E., Schreiber G., Toone M., Friesen J.D., Cashel M., Glaser G.
    J. Biol. Chem. 263:15699-15704(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Characterization of the hipA7 allele of Escherichia coli and evidence that high persistence is governed by (p)ppGpp synthesis."
    Korch S.B., Henderson T.A., Hill T.M.
    Mol. Microbiol. 50:1199-1213(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PERSISTENCE, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress."
    Boehm A., Steiner S., Zaehringer F., Casanova A., Hamburger F., Ritz D., Keck W., Ackermann M., Schirmer T., Jenal U.
    Mol. Microbiol. 72:1500-1516(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN BIOFILM FORMATION, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / AB400.

Entry informationi

Entry nameiRELA_ECOLI
AccessioniPrimary (citable) accession number: P0AG20
Secondary accession number(s): P11585, Q2MA48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3