Reviewed, UniProtKB/Swiss-Prot P0AG20 (RELA_ECOLI)

Names and origin

Protein names

Recommended name:
    GTP pyrophosphokinase
    EC=2.7.6.5
Alternative name(s):
    ATP:GTP 3'-pyrophosphotransferase
    ppGpp synthetase I
    (p)ppGpp synthetase

Gene names

Name:	relA
Ordered Locus Names:	b2784, JW2755

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp.
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.
Sequence similarities	Belongs to the relA/spoT family. Contains 1 ACT domain.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	guanosine tetraphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC amino acid binding Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 744	744	GTP pyrophosphokinase		PRO_0000166546
Regions
	Domain	667 – 741	75	ACT
Experimental info
	Sequence conflict	307	1	F → K in AAA03237. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0AG20-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: FA269709F15E1F25	FASTA	744	83,876
	10 20 30 40 50 60 MVAVRSAHIN KAGEFDPEKW IASLGITSQK SCECLAETWA YCLQQTQGHP DASLLLWRGV 70 80 90 100 110 120 EMVEILSTLS MDIDTLRAAL LFPLADANVV SEDVLRESVG KSVVNLIHGV RDMAAIRQLK 130 140 150 160 170 180 ATHTDSVSSE QVDNVRRMLL AMVDDFRCVV IKLAERIAHL REVKDAPEDE RVLAAKECTN 190 200 210 220 230 240 IYAPLANRLG IGQLKWELED YCFRYLHPTE YKRIAKLLHE RRLDREHYIE EFVGHLRAEM 250 260 270 280 290 300 KAEGVKAEVY GRPKHIYSIW RKMQKKNLAF DELFDVRAVR IVAERLQDCY AALGIVHTHY 310 320 330 340 350 360 RHLPDEFDDY VANPKPNGYQ SIHTVVLGPG GKTVEIQIRT KQMHEDAELG VAAHWKYKEG 370 380 390 400 410 420 AAAGGARSGH EDRIAWLRKL IAWQEEMADS GEMLDEVRSQ VFDDRVYVFT PKGDVVDLPA 430 440 450 460 470 480 GSTPLDFAYH IHSDVGHRCI GAKIGGRIVP FTYQLQMGDQ IEIITQKQPN PSRDWLNPNL 490 500 510 520 530 540 GYVTTSRGRS KIHAWFRKQD RDKNILAGRQ ILDDELEHLG ISLKEAEKHL LPRYNFNDVD 550 560 570 580 590 600 ELLAAIGGGD IRLNQMVNFL QSQFNKPSAE EQDAAALKQL QQKSYTPQNR SKDNGRVVVE 610 620 630 640 650 660 GVGNLMHHIA RCCQPIPGDE IVGFITQGRG ISVHRADCEQ LAELRSHAPE RIVDAVWGES 670 680 690 700 710 720 YSAGYSLVVR VVANDRSGLL RDITTILANE KVNVLGVASR SDTKQQLATI DMTIEIYNLQ 730 740 VLGRVLGKLN QVPDVIDARR LHGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence and characterization of the relA gene of Escherichia coli." Metzger S., Dror I.B., Aizenman E., Schreiber G., Toone M., Friesen J.D., Cashel M., Glaser G. J. Biol. Chem. 263:15699-15704(1988) [PubMed: 2844820] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04039 Unassigned DNA. Translation: AAA03237.1. U29580 Genomic DNA. Translation: AAA69294.1. U00096 Genomic DNA. Translation: AAC75826.1. AP009048 Genomic DNA. Translation: BAE76858.1.
PIR	KIECG. D65060.
RefSeq	AP_003350.1. NP_417264.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	947244.
GenomeReviews	Gene locus JW2755 in contig AP009048_GR. Gene locus b2784 in contig U00096_GR.
KEGG	ecj:JW2755. eco:b2784.
Organism-specific databases
EchoBASE	EB0828.
EcoGene	EG10835. relA.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AG20.
OMA	P0AG20. DLGHRCR.
Enzyme and pathway databases
BioCyc	EcoCyc:RELA-MON. MetaCyc:RELA-MON.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR012675. b-grasp_ferredoxin-like. IPR007685. RelA_SpoT. IPR004811. SpoT_relA. IPR004095. TGS. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00691. spoT_relA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RELA_ECOLI

Accession

Primary (citable) accession number: P0AG20
Secondary accession number(s): P11585, Q2MA48

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)