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P0AG20

- RELA_ECOLI

UniProt

P0AG20 - RELA_ECOLI

Protein

GTP pyrophosphokinase

Gene

relA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp.2 Publications

    Catalytic activityi

    ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

    Pathwayi

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. GTP binding Source: UniProtKB-KW
    4. GTP diphosphokinase activity Source: EcoCyc
    5. kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
    2. guanosine tetraphosphate metabolic process Source: EcoCyc
    3. nucleobase-containing small molecule interconversion Source: EcoCyc

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:RELA-MONOMER.
    ECOL316407:JW2755-MONOMER.
    MetaCyc:RELA-MONOMER.
    UniPathwayiUPA00908; UER00884.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP pyrophosphokinase (EC:2.7.6.5)
    Alternative name(s):
    (p)ppGpp synthase
    ATP:GTP 3'-pyrophosphotransferase
    ppGpp synthase I
    Gene namesi
    Name:relA
    Ordered Locus Names:b2784, JW2755
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10835. relA.

    Pathology & Biotechi

    Disruption phenotypei

    A slight increase in biofilm formation in a csrA-disrupted background; when combined with a spoT disruption (a ppGpp0 mutant) there is a very large increase in biofilm formation. Deletion of relA alone decreases persister cell formation in a hipA7 mutant; the double relA/spoT deletion obviates persister cell formation.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 744744GTP pyrophosphokinasePRO_0000166546Add
    BLAST

    Proteomic databases

    PaxDbiP0AG20.
    PRIDEiP0AG20.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AG20.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-10658N.
    IntActiP0AG20. 15 interactions.
    STRINGi511145.b2784.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AG20.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini668 – 74376ACTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RelA/SpoT family.Curated
    Contains 1 ACT domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0317.
    HOGENOMiHOG000018300.
    KOiK00951.
    OMAiTEIGHNC.
    OrthoDBiEOG6SV551.
    PhylomeDBiP0AG20.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view]
    SMARTiSM00954. RelA_SpoT. 1 hit.
    [Graphical view]
    SUPFAMiSSF81271. SSF81271. 1 hit.
    TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
    PROSITEiPS51671. ACT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AG20-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAVRSAHIN KAGEFDPEKW IASLGITSQK SCECLAETWA YCLQQTQGHP    50
    DASLLLWRGV EMVEILSTLS MDIDTLRAAL LFPLADANVV SEDVLRESVG 100
    KSVVNLIHGV RDMAAIRQLK ATHTDSVSSE QVDNVRRMLL AMVDDFRCVV 150
    IKLAERIAHL REVKDAPEDE RVLAAKECTN IYAPLANRLG IGQLKWELED 200
    YCFRYLHPTE YKRIAKLLHE RRLDREHYIE EFVGHLRAEM KAEGVKAEVY 250
    GRPKHIYSIW RKMQKKNLAF DELFDVRAVR IVAERLQDCY AALGIVHTHY 300
    RHLPDEFDDY VANPKPNGYQ SIHTVVLGPG GKTVEIQIRT KQMHEDAELG 350
    VAAHWKYKEG AAAGGARSGH EDRIAWLRKL IAWQEEMADS GEMLDEVRSQ 400
    VFDDRVYVFT PKGDVVDLPA GSTPLDFAYH IHSDVGHRCI GAKIGGRIVP 450
    FTYQLQMGDQ IEIITQKQPN PSRDWLNPNL GYVTTSRGRS KIHAWFRKQD 500
    RDKNILAGRQ ILDDELEHLG ISLKEAEKHL LPRYNFNDVD ELLAAIGGGD 550
    IRLNQMVNFL QSQFNKPSAE EQDAAALKQL QQKSYTPQNR SKDNGRVVVE 600
    GVGNLMHHIA RCCQPIPGDE IVGFITQGRG ISVHRADCEQ LAELRSHAPE 650
    RIVDAVWGES YSAGYSLVVR VVANDRSGLL RDITTILANE KVNVLGVASR 700
    SDTKQQLATI DMTIEIYNLQ VLGRVLGKLN QVPDVIDARR LHGS 744
    Length:744
    Mass (Da):83,876
    Last modified:December 20, 2005 - v1
    Checksum:iFA269709F15E1F25
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti307 – 3071F → K in AAA03237. (PubMed:2844820)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04039 Unassigned DNA. Translation: AAA03237.1.
    U29580 Genomic DNA. Translation: AAA69294.1.
    U00096 Genomic DNA. Translation: AAC75826.1.
    AP009048 Genomic DNA. Translation: BAE76858.1.
    PIRiD65060. KIECG.
    RefSeqiNP_417264.1. NC_000913.3.
    YP_490992.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75826; AAC75826; b2784.
    BAE76858; BAE76858; BAE76858.
    GeneIDi12930232.
    947244.
    KEGGiecj:Y75_p2721.
    eco:b2784.
    PATRICi32120984. VBIEscCol129921_2884.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04039 Unassigned DNA. Translation: AAA03237.1 .
    U29580 Genomic DNA. Translation: AAA69294.1 .
    U00096 Genomic DNA. Translation: AAC75826.1 .
    AP009048 Genomic DNA. Translation: BAE76858.1 .
    PIRi D65060. KIECG.
    RefSeqi NP_417264.1. NC_000913.3.
    YP_490992.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AG20.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10658N.
    IntActi P0AG20. 15 interactions.
    STRINGi 511145.b2784.

    Chemistry

    ChEMBLi CHEMBL1163114.

    Proteomic databases

    PaxDbi P0AG20.
    PRIDEi P0AG20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75826 ; AAC75826 ; b2784 .
    BAE76858 ; BAE76858 ; BAE76858 .
    GeneIDi 12930232.
    947244.
    KEGGi ecj:Y75_p2721.
    eco:b2784.
    PATRICi 32120984. VBIEscCol129921_2884.

    Organism-specific databases

    EchoBASEi EB0828.
    EcoGenei EG10835. relA.

    Phylogenomic databases

    eggNOGi COG0317.
    HOGENOMi HOG000018300.
    KOi K00951.
    OMAi TEIGHNC.
    OrthoDBi EOG6SV551.
    PhylomeDBi P0AG20.

    Enzyme and pathway databases

    UniPathwayi UPA00908 ; UER00884 .
    BioCyci EcoCyc:RELA-MONOMER.
    ECOL316407:JW2755-MONOMER.
    MetaCyc:RELA-MONOMER.

    Miscellaneous databases

    PROi P0AG20.

    Gene expression databases

    Genevestigatori P0AG20.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view ]
    SMARTi SM00954. RelA_SpoT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81271. SSF81271. 1 hit.
    TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
    PROSITEi PS51671. ACT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence and characterization of the relA gene of Escherichia coli."
      Metzger S., Dror I.B., Aizenman E., Schreiber G., Toone M., Friesen J.D., Cashel M., Glaser G.
      J. Biol. Chem. 263:15699-15704(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Characterization of the hipA7 allele of Escherichia coli and evidence that high persistence is governed by (p)ppGpp synthesis."
      Korch S.B., Henderson T.A., Hill T.M.
      Mol. Microbiol. 50:1199-1213(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PERSISTENCE, DISRUPTION PHENOTYPE.
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress."
      Boehm A., Steiner S., Zaehringer F., Casanova A., Hamburger F., Ritz D., Keck W., Ackermann M., Schirmer T., Jenal U.
      Mol. Microbiol. 72:1500-1516(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN BIOFILM FORMATION, DISRUPTION PHENOTYPE.
      Strain: K12 / MG1655 / AB400.

    Entry informationi

    Entry nameiRELA_ECOLI
    AccessioniPrimary (citable) accession number: P0AG20
    Secondary accession number(s): P11585, Q2MA48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3