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P0AG20 (RELA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP pyrophosphokinase

EC=2.7.6.5
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene names
Name:relA
Ordered Locus Names:b2784, JW2755
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp. Ref.4 Ref.5

Catalytic activity

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathway

Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.

Disruption phenotype

A slight increase in biofilm formation in a csrA-disrupted background; when combined with a spoT disruption (a ppGpp0 mutant) there is a very large increase in biofilm formation. Deletion of relA alone decreases persister cell formation in a hipA7 mutant; the double relA/spoT deletion obviates persister cell formation. Ref.4 Ref.5

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 ACT domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 744744GTP pyrophosphokinase
PRO_0000166546

Regions

Domain668 – 74376ACT

Experimental info

Sequence conflict3071F → K in AAA03237. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0AG20 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: FA269709F15E1F25

FASTA74483,876
        10         20         30         40         50         60 
MVAVRSAHIN KAGEFDPEKW IASLGITSQK SCECLAETWA YCLQQTQGHP DASLLLWRGV 

        70         80         90        100        110        120 
EMVEILSTLS MDIDTLRAAL LFPLADANVV SEDVLRESVG KSVVNLIHGV RDMAAIRQLK 

       130        140        150        160        170        180 
ATHTDSVSSE QVDNVRRMLL AMVDDFRCVV IKLAERIAHL REVKDAPEDE RVLAAKECTN 

       190        200        210        220        230        240 
IYAPLANRLG IGQLKWELED YCFRYLHPTE YKRIAKLLHE RRLDREHYIE EFVGHLRAEM 

       250        260        270        280        290        300 
KAEGVKAEVY GRPKHIYSIW RKMQKKNLAF DELFDVRAVR IVAERLQDCY AALGIVHTHY 

       310        320        330        340        350        360 
RHLPDEFDDY VANPKPNGYQ SIHTVVLGPG GKTVEIQIRT KQMHEDAELG VAAHWKYKEG 

       370        380        390        400        410        420 
AAAGGARSGH EDRIAWLRKL IAWQEEMADS GEMLDEVRSQ VFDDRVYVFT PKGDVVDLPA 

       430        440        450        460        470        480 
GSTPLDFAYH IHSDVGHRCI GAKIGGRIVP FTYQLQMGDQ IEIITQKQPN PSRDWLNPNL 

       490        500        510        520        530        540 
GYVTTSRGRS KIHAWFRKQD RDKNILAGRQ ILDDELEHLG ISLKEAEKHL LPRYNFNDVD 

       550        560        570        580        590        600 
ELLAAIGGGD IRLNQMVNFL QSQFNKPSAE EQDAAALKQL QQKSYTPQNR SKDNGRVVVE 

       610        620        630        640        650        660 
GVGNLMHHIA RCCQPIPGDE IVGFITQGRG ISVHRADCEQ LAELRSHAPE RIVDAVWGES 

       670        680        690        700        710        720 
YSAGYSLVVR VVANDRSGLL RDITTILANE KVNVLGVASR SDTKQQLATI DMTIEIYNLQ 

       730        740 
VLGRVLGKLN QVPDVIDARR LHGS 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence and characterization of the relA gene of Escherichia coli."
Metzger S., Dror I.B., Aizenman E., Schreiber G., Toone M., Friesen J.D., Cashel M., Glaser G.
J. Biol. Chem. 263:15699-15704(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Characterization of the hipA7 allele of Escherichia coli and evidence that high persistence is governed by (p)ppGpp synthesis."
Korch S.B., Henderson T.A., Hill T.M.
Mol. Microbiol. 50:1199-1213(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN PERSISTENCE, DISRUPTION PHENOTYPE.
Strain: K12 / MG1655 / ATCC 47076.
[5]"Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress."
Boehm A., Steiner S., Zaehringer F., Casanova A., Hamburger F., Ritz D., Keck W., Ackermann M., Schirmer T., Jenal U.
Mol. Microbiol. 72:1500-1516(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN BIOFILM FORMATION, DISRUPTION PHENOTYPE.
Strain: K12 / MG1655 / AB400.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04039 Unassigned DNA. Translation: AAA03237.1.
U29580 Genomic DNA. Translation: AAA69294.1.
U00096 Genomic DNA. Translation: AAC75826.1.
AP009048 Genomic DNA. Translation: BAE76858.1.
PIRKIECG. D65060.
RefSeqNP_417264.1. NC_000913.3.
YP_490992.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AG20.
SMRP0AG20. Positions 16-465, 663-741.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10658N.
IntActP0AG20. 15 interactions.
STRING511145.b2784.

Chemistry

ChEMBLCHEMBL1163114.

Proteomic databases

PaxDbP0AG20.
PRIDEP0AG20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75826; AAC75826; b2784.
BAE76858; BAE76858; BAE76858.
GeneID12930232.
947244.
KEGGecj:Y75_p2721.
eco:b2784.
PATRIC32120984. VBIEscCol129921_2884.

Organism-specific databases

EchoBASEEB0828.
EcoGeneEG10835. relA.

Phylogenomic databases

eggNOGCOG0317.
HOGENOMHOG000018300.
KOK00951.
OMATEIGHNC.
OrthoDBEOG6SV551.
PhylomeDBP0AG20.
ProtClustDBPRK10872.

Enzyme and pathway databases

BioCycEcoCyc:RELA-MONOMER.
ECOL316407:JW2755-MONOMER.
MetaCyc:RELA-MONOMER.
UniPathwayUPA00908; UER00884.

Gene expression databases

GenevestigatorP0AG20.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR026020. (p)ppGpp_Synthase.
IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PANTHERPTHR21262:SF1. PTHR21262:SF1. 1 hit.
PfamPF01842. ACT. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTSM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMSSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0AG20.

Entry information

Entry nameRELA_ECOLI
AccessionPrimary (citable) accession number: P0AG20
Secondary accession number(s): P11585, Q2MA48
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene