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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF), Amidophosphoribosyltransferase (purF), Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD), Phosphoribosylamine--glycine ligase (purD), Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2NucleophileUniRule annotation1
Metal bindingi305MagnesiumUniRule annotation1
Metal bindingi367MagnesiumUniRule annotation1
Metal bindingi368MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124

Protein family/group databases

MEROPSiC44.001

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:SF2388, S2523
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001396422 – 505AmidophosphoribosyltransferaseAdd BLAST504

Proteomic databases

PRIDEiP0AG17

Structurei

3D structure databases

ProteinModelPortaliP0AG17
SMRiP0AG17
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 236Glutamine amidotransferase type-2UniRule annotationAdd BLAST235

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CBA Bacteria
COG0034 LUCA
KOiK00764
OMAiFRPLCLG

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.10, 2 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AG17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGIAGV MPVNQSIYDA LTVLQHRGQD AAGIITIDAN NCFRLRKANG
60 70 80 90 100
LVSDVFEARH MQRLQGNMGI GHVRYPTAGS SSASEAQPFY VNSPYGITLA
110 120 130 140 150
HNGNLTNAHE LRKKLFEEKR RHINTTSDSE ILLNIFASEL DNFRHYPLEA
160 170 180 190 200
DNIFAAIAAT NRLIRGAYAC VAMIIGHGMV AFRDPNGIRP LVLGKRDIDE
210 220 230 240 250
NRTEYMVASE SVALDTLGFD FLRDVAPGEA IYITEEGQLF TRQCADNPVS
260 270 280 290 300
NPCLFEYVYF ARPDSFIDKI SVYSARVNMG TKLGEKIARE WEDLDIDVVI
310 320 330 340 350
PIPETSCDIA LEIARILGKP YRQGFVKNRY VGRTFIMPGQ QLRRKSVRRK
360 370 380 390 400
LNANRAEFRD KNVLLVDDSI VRGTTSEQII EMAREAGAKK VYLASAAPEI
410 420 430 440 450
RFPNVYGIDM PSATELIAHG REVDEIRQII GADGLIFQDL NDLIDAVRAE
460 470 480 490 500
NPDIQQFECS VFNGVYVTKD VDQGYLDFLD TLRNDDAKAV QRQNEVENLE

MHNEG
Length:505
Mass (Da):56,488
Last modified:January 23, 2007 - v2
Checksum:i50FD08EA85E6F2A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA Translation: AAN43901.1
AE014073 Genomic DNA Translation: AAP17719.1
RefSeqiNP_708194.1, NC_004337.2
WP_000334220.1, NZ_NIYV01000063.1

Genome annotation databases

EnsemblBacteriaiAAN43901; AAN43901; SF2388
AAP17719; AAP17719; S2523
GeneIDi1025528
KEGGisfl:SF2388
sfx:S2523
PATRICifig|198214.7.peg.2855

Similar proteinsi

Entry informationi

Entry nameiPUR1_SHIFL
AccessioniPrimary (citable) accession number: P0AG17
Secondary accession number(s): P00496
, P78092, P78191, P78192, Q47255, Q59425
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health