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Protein

Probable protease SohB

Gene

sohB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Multicopy suppressor of the HtrA (DegP) null phenotype. It is possibly a protease, not essential for bacterial viability.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei178 – 1781NucleophileBy similarity
Active sitei230 – 2301Proton donor/acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciEcoCyc:EG10956-MONOMER.
ECOL316407:JW1264-MONOMER.

Protein family/group databases

MEROPSiS49.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protease SohB (EC:3.4.21.-)
Gene namesi
Name:sohB
Ordered Locus Names:b1272, JW1264
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10956. sohB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88PeriplasmicSequence analysis
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Topological domaini30 – 349320CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Probable protease SohBPRO_0000171448Add
BLAST

Proteomic databases

PaxDbiP0AG14.
PRIDEiP0AG14.

Interactioni

Protein-protein interaction databases

BioGridi4259577. 7 interactions.
DIPiDIP-35930N.
IntActiP0AG14. 3 interactions.
STRINGi511145.b1272.

Structurei

3D structure databases

ProteinModelPortaliP0AG14.
SMRiP0AG14. Positions 101-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S49 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105ERU. Bacteria.
COG0616. LUCA.
HOGENOMiHOG000281079.
InParanoidiP0AG14.
KOiK04774.
OMAiMACIGEK.
PhylomeDBiP0AG14.

Family and domain databases

Gene3Di3.90.226.10. 2 hits.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR002142. Peptidase_S49.
IPR013703. Peptidase_S49_N_proteobac.
[Graphical view]
PfamiPF01343. Peptidase_S49. 1 hit.
PF08496. Peptidase_S49_N. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 2 hits.

Sequencei

Sequence statusi: Complete.

P0AG14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLSEYGLF LAKIVTVVLA IAAIAAIIVN VAQRNKRQRG ELRVNNLSEQ
60 70 80 90 100
YKEMKEELAA ALMDSHQQKQ WHKAQKKKHK QEAKAAKAKA KLGEVATDSK
110 120 130 140 150
PRVWVLDFKG SMDAHEVNSL REEITAVLAA FKPQDQVVLR LESPGGMVHG
160 170 180 190 200
YGLAASQLQR LRDKNIPLTV TVDKVAASGG YMMACVADKI VSAPFAIVGS
210 220 230 240 250
IGVVAQMPNF NRFLKSKDID IELHTAGQYK RTLTLLGENT EEGREKFREE
260 270 280 290 300
LNETHQLFKD FVKRMRPSLD IEQVATGEHW YGQQAVEKGL VDEINTSDEV
310 320 330 340
ILSLMEGREV VNVRYMQRKR LIDRFTGSAA ESADRLLLRW WQRGQKPLM
Length:349
Mass (Da):39,366
Last modified:December 20, 2005 - v1
Checksum:i3362AE74C33C5FFC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851A → R in AAA24639 (PubMed:1885549).Curated
Sequence conflicti90 – 901A → R in AAA24639 (PubMed:1885549).Curated
Sequence conflicti133 – 1386PQDQVV → HQPQVA (PubMed:3029379).Curated
Sequence conflicti143 – 15412SPGGM…GYGLA → ALVAWCMLRAG (PubMed:3029379).CuratedAdd
BLAST
Sequence conflicti164 – 1641K → R (PubMed:3029379).Curated
Sequence conflicti245 – 25814EKFRE…THQLF → GKIPRRVERNASVV (PubMed:3029379).CuratedAdd
BLAST
Sequence conflicti296 – 2961T → A in AAA24639 (PubMed:1885549).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73320 Genomic DNA. Translation: AAA24639.1.
U00096 Genomic DNA. Translation: AAC74354.1.
AP009048 Genomic DNA. Translation: BAA14809.1.
PIRiC64875.
RefSeqiNP_415788.1. NC_000913.3.
WP_000422045.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74354; AAC74354; b1272.
BAA14809; BAA14809; BAA14809.
GeneIDi945858.
KEGGiecj:JW1264.
eco:b1272.
PATRICi32117802. VBIEscCol129921_1321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73320 Genomic DNA. Translation: AAA24639.1.
U00096 Genomic DNA. Translation: AAC74354.1.
AP009048 Genomic DNA. Translation: BAA14809.1.
PIRiC64875.
RefSeqiNP_415788.1. NC_000913.3.
WP_000422045.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AG14.
SMRiP0AG14. Positions 101-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259577. 7 interactions.
DIPiDIP-35930N.
IntActiP0AG14. 3 interactions.
STRINGi511145.b1272.

Protein family/group databases

MEROPSiS49.002.

Proteomic databases

PaxDbiP0AG14.
PRIDEiP0AG14.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74354; AAC74354; b1272.
BAA14809; BAA14809; BAA14809.
GeneIDi945858.
KEGGiecj:JW1264.
eco:b1272.
PATRICi32117802. VBIEscCol129921_1321.

Organism-specific databases

EchoBASEiEB0949.
EcoGeneiEG10956. sohB.

Phylogenomic databases

eggNOGiENOG4105ERU. Bacteria.
COG0616. LUCA.
HOGENOMiHOG000281079.
InParanoidiP0AG14.
KOiK04774.
OMAiMACIGEK.
PhylomeDBiP0AG14.

Enzyme and pathway databases

BioCyciEcoCyc:EG10956-MONOMER.
ECOL316407:JW1264-MONOMER.

Miscellaneous databases

PROiP0AG14.

Family and domain databases

Gene3Di3.90.226.10. 2 hits.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR002142. Peptidase_S49.
IPR013703. Peptidase_S49_N_proteobac.
[Graphical view]
PfamiPF01343. Peptidase_S49. 1 hit.
PF08496. Peptidase_S49_N. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSOHB_ECOLI
AccessioniPrimary (citable) accession number: P0AG14
Secondary accession number(s): P24213, P77676
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.