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Protein

Probable protease SohB

Gene

sohB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multicopy suppressor of the HtrA (DegP) null phenotype. It is possibly a protease, not essential for bacterial viability.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei178NucleophileBy similarity1
Active sitei230Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciEcoCyc:EG10956-MONOMER

Protein family/group databases

MEROPSiS49.002

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protease SohB (EC:3.4.21.-)
Gene namesi
Name:sohB
Ordered Locus Names:b1272, JW1264
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG10956 sohB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8PeriplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 349CytoplasmicSequence analysisAdd BLAST320

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714481 – 349Probable protease SohBAdd BLAST349

Proteomic databases

PaxDbiP0AG14
PRIDEiP0AG14

Interactioni

Protein-protein interaction databases

BioGridi4259577, 67 interactors
DIPiDIP-35930N
IntActiP0AG14, 3 interactors
STRINGi316385.ECDH10B_1387

Structurei

3D structure databases

ProteinModelPortaliP0AG14
SMRiP0AG14
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S49 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105ERU Bacteria
COG0616 LUCA
HOGENOMiHOG000281079
InParanoidiP0AG14
KOiK04774
OMAiFLYEYGL
PhylomeDBiP0AG14

Family and domain databases

InterProiView protein in InterPro
IPR029045 ClpP/crotonase-like_dom_sf
IPR002142 Peptidase_S49
IPR013703 Peptidase_S49_N_proteobac
PfamiView protein in Pfam
PF01343 Peptidase_S49, 1 hit
PF08496 Peptidase_S49_N, 1 hit
SUPFAMiSSF52096 SSF52096, 2 hits

Sequencei

Sequence statusi: Complete.

P0AG14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLSEYGLF LAKIVTVVLA IAAIAAIIVN VAQRNKRQRG ELRVNNLSEQ
60 70 80 90 100
YKEMKEELAA ALMDSHQQKQ WHKAQKKKHK QEAKAAKAKA KLGEVATDSK
110 120 130 140 150
PRVWVLDFKG SMDAHEVNSL REEITAVLAA FKPQDQVVLR LESPGGMVHG
160 170 180 190 200
YGLAASQLQR LRDKNIPLTV TVDKVAASGG YMMACVADKI VSAPFAIVGS
210 220 230 240 250
IGVVAQMPNF NRFLKSKDID IELHTAGQYK RTLTLLGENT EEGREKFREE
260 270 280 290 300
LNETHQLFKD FVKRMRPSLD IEQVATGEHW YGQQAVEKGL VDEINTSDEV
310 320 330 340
ILSLMEGREV VNVRYMQRKR LIDRFTGSAA ESADRLLLRW WQRGQKPLM
Length:349
Mass (Da):39,366
Last modified:December 20, 2005 - v1
Checksum:i3362AE74C33C5FFC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85A → R in AAA24639 (PubMed:1885549).Curated1
Sequence conflicti90A → R in AAA24639 (PubMed:1885549).Curated1
Sequence conflicti133 – 138PQDQVV → HQPQVA (PubMed:3029379).Curated6
Sequence conflicti143 – 154SPGGM…GYGLA → ALVAWCMLRAG (PubMed:3029379).CuratedAdd BLAST12
Sequence conflicti164K → R (PubMed:3029379).Curated1
Sequence conflicti245 – 258EKFRE…THQLF → GKIPRRVERNASVV (PubMed:3029379).CuratedAdd BLAST14
Sequence conflicti296T → A in AAA24639 (PubMed:1885549).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73320 Genomic DNA Translation: AAA24639.1
U00096 Genomic DNA Translation: AAC74354.1
AP009048 Genomic DNA Translation: BAA14809.1
PIRiC64875
RefSeqiNP_415788.1, NC_000913.3
WP_000422045.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74354; AAC74354; b1272
BAA14809; BAA14809; BAA14809
GeneIDi945858
KEGGiecj:JW1264
eco:b1272
PATRICifig|1411691.4.peg.1012

Entry informationi

Entry nameiSOHB_ECOLI
AccessioniPrimary (citable) accession number: P0AG14
Secondary accession number(s): P24213, P77676
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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