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P0AG11

- UMUD_ECOLI

UniProt

P0AG11 - UMUD_ECOLI

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Protein

Protein UmuD

Gene

umuD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei24 – 252Cleavage; by autolysis
Active sitei60 – 601For autocatalytic cleavage activity
Active sitei97 – 971For autocatalytic cleavage activity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed DNA polymerase activity Source: EcoCyc
  3. serine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: EcoliWiki
  2. DNA-dependent DNA replication Source: GOC
  3. DNA repair Source: EcoCyc
  4. regulation of transcription, DNA-templated Source: InterPro
  5. SOS response Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

DNA damage, DNA repair, SOS mutagenesis, SOS response

Enzyme and pathway databases

BioCyciEcoCyc:EG11057-MONOMER.
ECOL316407:JW1172-MONOMER.
MetaCyc:EG11057-MONOMER.

Protein family/group databases

MEROPSiS24.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein UmuD (EC:3.4.21.-)
Cleaved into the following chain:
Gene namesi
Name:umuD
Ordered Locus Names:b1183, JW1172
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11057. umuD.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271P → D in umuD1; non-cleavable. 1 Publication
Mutagenesisi65 – 651G → R in umuD44; non-cleavable. 1 Publication
Mutagenesisi92 – 921G → D in umuD77; non-cleavable. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 139139Protein UmuDPRO_0000041988Add
BLAST
Chaini25 – 139115Protein UmuD'PRO_0000027305Add
BLAST

Keywords - PTMi

Autocatalytic cleavage

Expressioni

Gene expression databases

GenevestigatoriP0AG11.

Interactioni

Protein-protein interaction databases

DIPiDIP-29679N.
IntActiP0AG11. 13 interactions.
STRINGi511145.b1183.

Structurei

Secondary structure

1
139
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi40 – 445
Helixi48 – 503
Beta strandi51 – 555
Beta strandi58 – 603
Helixi62 – 643
Beta strandi71 – 755
Beta strandi82 – 843
Beta strandi85 – 906
Beta strandi93 – 1008
Beta strandi102 – 1043
Beta strandi106 – 1083
Beta strandi120 – 1245
Beta strandi125 – 13511

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AY9X-ray3.00A/B32-139[»]
1I4VNMR-A/B26-139[»]
1UMUX-ray2.50A/B25-137[»]
DisProtiDP00626.
ProteinModelPortaliP0AG11.
SMRiP0AG11. Positions 32-139.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AG11.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S24 family.Curated

Phylogenomic databases

eggNOGiCOG1974.
HOGENOMiHOG000232166.
InParanoidiP0AG11.
KOiK03503.
OMAiFMERVSA.
OrthoDBiEOG6JHRHJ.
PhylomeDBiP0AG11.

Family and domain databases

Gene3Di2.10.109.10. 1 hit.
InterProiIPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PfamiPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AG11-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLFIKPADLR EIVTFPLFSD LVQCGFPSPA ADYVEQRIDL NQLLIQHPSA
60 70 80 90 100
TYFVKASGDS MIDGGISDGD LLIVDSAITA SHGDIVIAAV DGEFTVKKLQ
110 120 130
LRPTVQLIPM NSAYSPITIS SEDTLDVFGV VIHVVKAMR
Length:139
Mass (Da):15,063
Last modified:November 1, 1986 - v1
Checksum:i0681A3FFAC7ED583
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10107 Genomic DNA. Translation: AAA24728.1.
M13387 Genomic DNA. Translation: AAA98073.1.
U00096 Genomic DNA. Translation: AAC74267.1.
AP009048 Genomic DNA. Translation: BAA36030.1.
PIRiA03551. ZWECD.
RefSeqiNP_415701.1. NC_000913.3.
YP_489450.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74267; AAC74267; b1183.
BAA36030; BAA36030; BAA36030.
GeneIDi12932823.
945746.
KEGGiecj:Y75_p1155.
eco:b1183.
PATRICi32117612. VBIEscCol129921_1228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10107 Genomic DNA. Translation: AAA24728.1 .
M13387 Genomic DNA. Translation: AAA98073.1 .
U00096 Genomic DNA. Translation: AAC74267.1 .
AP009048 Genomic DNA. Translation: BAA36030.1 .
PIRi A03551. ZWECD.
RefSeqi NP_415701.1. NC_000913.3.
YP_489450.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AY9 X-ray 3.00 A/B 32-139 [» ]
1I4V NMR - A/B 26-139 [» ]
1UMU X-ray 2.50 A/B 25-137 [» ]
DisProti DP00626.
ProteinModelPortali P0AG11.
SMRi P0AG11. Positions 32-139.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29679N.
IntActi P0AG11. 13 interactions.
STRINGi 511145.b1183.

Protein family/group databases

MEROPSi S24.003.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74267 ; AAC74267 ; b1183 .
BAA36030 ; BAA36030 ; BAA36030 .
GeneIDi 12932823.
945746.
KEGGi ecj:Y75_p1155.
eco:b1183.
PATRICi 32117612. VBIEscCol129921_1228.

Organism-specific databases

EchoBASEi EB1050.
EcoGenei EG11057. umuD.

Phylogenomic databases

eggNOGi COG1974.
HOGENOMi HOG000232166.
InParanoidi P0AG11.
KOi K03503.
OMAi FMERVSA.
OrthoDBi EOG6JHRHJ.
PhylomeDBi P0AG11.

Enzyme and pathway databases

BioCyci EcoCyc:EG11057-MONOMER.
ECOL316407:JW1172-MONOMER.
MetaCyc:EG11057-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AG11.
PROi P0AG11.

Gene expression databases

Genevestigatori P0AG11.

Family and domain databases

Gene3Di 2.10.109.10. 1 hit.
InterProi IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view ]
Pfami PF00717. Peptidase_S24. 1 hit.
[Graphical view ]
PRINTSi PR00726. LEXASERPTASE.
SUPFAMi SSF51306. SSF51306. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli."
    Kitagawa Y., Akaboshi E., Shinagawa H., Horii T., Ogawa H., Kato T.
    Proc. Natl. Acad. Sci. U.S.A. 82:4336-4340(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology."
    Perry K.L., Elledge S.J., Mitchell B.B., Marsh L., Walker G.C.
    Proc. Natl. Acad. Sci. U.S.A. 82:4331-4335(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Escherichia coli umuDC mutants: DNA sequence alterations and UmuD cleavage."
    Koch W.H., Ennis D.G., Levine A.S., Woodgate R.
    Mol. Gen. Genet. 233:443-448(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  7. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  8. "Structure of the UmuD' protein and its regulation in response to DNA damage."
    Peat T.S., Frank E., McDonald J.P., Levine A.S., Woodgate R., Hendrickson W.A.
    Nature 380:727-730(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF UMUD'.

Entry informationi

Entry nameiUMUD_ECOLI
AccessioniPrimary (citable) accession number: P0AG11
Secondary accession number(s): P04153
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3