Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0AG11

- UMUD_ECOLI

UniProt

P0AG11 - UMUD_ECOLI

Protein

Protein UmuD

Gene

umuD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei24 – 252Cleavage; by autolysis
    Active sitei60 – 601For autocatalytic cleavage activity
    Active sitei97 – 971For autocatalytic cleavage activity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed DNA polymerase activity Source: EcoCyc
    3. protein binding Source: EcoliWiki
    4. serine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: EcoliWiki
    2. DNA-dependent DNA replication Source: GOC
    3. DNA repair Source: EcoCyc
    4. regulation of transcription, DNA-templated Source: InterPro
    5. SOS response Source: EcoCyc

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    DNA damage, DNA repair, SOS mutagenesis, SOS response

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11057-MONOMER.
    ECOL316407:JW1172-MONOMER.
    MetaCyc:EG11057-MONOMER.

    Protein family/group databases

    MEROPSiS24.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein UmuD (EC:3.4.21.-)
    Cleaved into the following chain:
    Gene namesi
    Name:umuD
    Ordered Locus Names:b1183, JW1172
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11057. umuD.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi27 – 271P → D in umuD1; non-cleavable. 1 Publication
    Mutagenesisi65 – 651G → R in umuD44; non-cleavable. 1 Publication
    Mutagenesisi92 – 921G → D in umuD77; non-cleavable. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 139139Protein UmuDPRO_0000041988Add
    BLAST
    Chaini25 – 139115Protein UmuD'PRO_0000027305Add
    BLAST

    Keywords - PTMi

    Autocatalytic cleavage

    Expressioni

    Gene expression databases

    GenevestigatoriP0AG11.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-29679N.
    IntActiP0AG11. 13 interactions.
    STRINGi511145.b1183.

    Structurei

    Secondary structure

    1
    139
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi40 – 445
    Helixi48 – 503
    Beta strandi51 – 555
    Beta strandi58 – 603
    Helixi62 – 643
    Beta strandi71 – 755
    Beta strandi82 – 843
    Beta strandi85 – 906
    Beta strandi93 – 1008
    Beta strandi102 – 1043
    Beta strandi106 – 1083
    Beta strandi120 – 1245
    Beta strandi125 – 13511

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AY9X-ray3.00A/B32-139[»]
    1I4VNMR-A/B26-139[»]
    1UMUX-ray2.50A/B25-137[»]
    DisProtiDP00626.
    ProteinModelPortaliP0AG11.
    SMRiP0AG11. Positions 32-139.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AG11.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S24 family.Curated

    Phylogenomic databases

    eggNOGiCOG1974.
    HOGENOMiHOG000232166.
    KOiK03503.
    OMAiFMERVSA.
    OrthoDBiEOG6JHRHJ.
    PhylomeDBiP0AG11.

    Family and domain databases

    Gene3Di2.10.109.10. 1 hit.
    InterProiIPR028360. Peptidase_S24/S26_b-rbn.
    IPR006197. Peptidase_S24_LexA.
    IPR019759. Peptidase_S24_S26.
    IPR015927. Peptidase_S24_S26A/B/C.
    [Graphical view]
    PfamiPF00717. Peptidase_S24. 1 hit.
    [Graphical view]
    PRINTSiPR00726. LEXASERPTASE.
    SUPFAMiSSF51306. SSF51306. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AG11-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLFIKPADLR EIVTFPLFSD LVQCGFPSPA ADYVEQRIDL NQLLIQHPSA    50
    TYFVKASGDS MIDGGISDGD LLIVDSAITA SHGDIVIAAV DGEFTVKKLQ 100
    LRPTVQLIPM NSAYSPITIS SEDTLDVFGV VIHVVKAMR 139
    Length:139
    Mass (Da):15,063
    Last modified:November 1, 1986 - v1
    Checksum:i0681A3FFAC7ED583
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10107 Genomic DNA. Translation: AAA24728.1.
    M13387 Genomic DNA. Translation: AAA98073.1.
    U00096 Genomic DNA. Translation: AAC74267.1.
    AP009048 Genomic DNA. Translation: BAA36030.1.
    PIRiA03551. ZWECD.
    RefSeqiNP_415701.1. NC_000913.3.
    YP_489450.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74267; AAC74267; b1183.
    BAA36030; BAA36030; BAA36030.
    GeneIDi12932823.
    945746.
    KEGGiecj:Y75_p1155.
    eco:b1183.
    PATRICi32117612. VBIEscCol129921_1228.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10107 Genomic DNA. Translation: AAA24728.1 .
    M13387 Genomic DNA. Translation: AAA98073.1 .
    U00096 Genomic DNA. Translation: AAC74267.1 .
    AP009048 Genomic DNA. Translation: BAA36030.1 .
    PIRi A03551. ZWECD.
    RefSeqi NP_415701.1. NC_000913.3.
    YP_489450.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AY9 X-ray 3.00 A/B 32-139 [» ]
    1I4V NMR - A/B 26-139 [» ]
    1UMU X-ray 2.50 A/B 25-137 [» ]
    DisProti DP00626.
    ProteinModelPortali P0AG11.
    SMRi P0AG11. Positions 32-139.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29679N.
    IntActi P0AG11. 13 interactions.
    STRINGi 511145.b1183.

    Protein family/group databases

    MEROPSi S24.003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74267 ; AAC74267 ; b1183 .
    BAA36030 ; BAA36030 ; BAA36030 .
    GeneIDi 12932823.
    945746.
    KEGGi ecj:Y75_p1155.
    eco:b1183.
    PATRICi 32117612. VBIEscCol129921_1228.

    Organism-specific databases

    EchoBASEi EB1050.
    EcoGenei EG11057. umuD.

    Phylogenomic databases

    eggNOGi COG1974.
    HOGENOMi HOG000232166.
    KOi K03503.
    OMAi FMERVSA.
    OrthoDBi EOG6JHRHJ.
    PhylomeDBi P0AG11.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11057-MONOMER.
    ECOL316407:JW1172-MONOMER.
    MetaCyc:EG11057-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AG11.
    PROi P0AG11.

    Gene expression databases

    Genevestigatori P0AG11.

    Family and domain databases

    Gene3Di 2.10.109.10. 1 hit.
    InterProi IPR028360. Peptidase_S24/S26_b-rbn.
    IPR006197. Peptidase_S24_LexA.
    IPR019759. Peptidase_S24_S26.
    IPR015927. Peptidase_S24_S26A/B/C.
    [Graphical view ]
    Pfami PF00717. Peptidase_S24. 1 hit.
    [Graphical view ]
    PRINTSi PR00726. LEXASERPTASE.
    SUPFAMi SSF51306. SSF51306. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli."
      Kitagawa Y., Akaboshi E., Shinagawa H., Horii T., Ogawa H., Kato T.
      Proc. Natl. Acad. Sci. U.S.A. 82:4336-4340(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology."
      Perry K.L., Elledge S.J., Mitchell B.B., Marsh L., Walker G.C.
      Proc. Natl. Acad. Sci. U.S.A. 82:4331-4335(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Escherichia coli umuDC mutants: DNA sequence alterations and UmuD cleavage."
      Koch W.H., Ennis D.G., Levine A.S., Woodgate R.
      Mol. Gen. Genet. 233:443-448(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    7. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.
    8. "Structure of the UmuD' protein and its regulation in response to DNA damage."
      Peat T.S., Frank E., McDonald J.P., Levine A.S., Woodgate R., Hendrickson W.A.
      Nature 380:727-730(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF UMUD'.

    Entry informationi

    Entry nameiUMUD_ECOLI
    AccessioniPrimary (citable) accession number: P0AG11
    Secondary accession number(s): P04153
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: November 1, 1986
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3