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Protein

Protein UmuD

Gene

umuD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60For autocatalytic cleavage activity1
Active sitei97For autocatalytic cleavage activity1

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed DNA polymerase activity Source: EcoCyc
  • serine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA repair Source: EcoCyc
  • regulation of transcription, DNA-templated Source: InterPro
  • SOS response Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processDNA damage, DNA repair, SOS mutagenesis, SOS response

Enzyme and pathway databases

BioCyciEcoCyc:EG11057-MONOMER.
MetaCyc:EG11057-MONOMER.
BRENDAi3.4.21.B30. 2026.

Protein family/group databases

MEROPSiS24.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein UmuD (EC:3.4.21.-)
Cleaved into the following chain:
Gene namesi
Name:umuD
Ordered Locus Names:b1183, JW1172
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11057. umuD.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27P → D in umuD1; non-cleavable. 1 Publication1
Mutagenesisi65G → R in umuD44; non-cleavable. 1 Publication1
Mutagenesisi92G → D in umuD77; non-cleavable. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000419881 – 139Protein UmuDAdd BLAST139
ChainiPRO_000002730525 – 139Protein UmuD'Add BLAST115

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei24 – 25Cleavage; by autolysis2

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PaxDbiP0AG11.
PRIDEiP0AG11.

Expressioni

Inductioni

Induced 1.5-fold by hydroxyurea.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261817. 117 interactors.
DIPiDIP-29679N.
IntActiP0AG11. 13 interactors.
STRINGi316385.ECDH10B_1236.

Structurei

Secondary structure

1139
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 44Combined sources5
Helixi48 – 50Combined sources3
Beta strandi51 – 55Combined sources5
Beta strandi58 – 60Combined sources3
Helixi62 – 64Combined sources3
Beta strandi71 – 75Combined sources5
Beta strandi82 – 84Combined sources3
Beta strandi85 – 90Combined sources6
Beta strandi93 – 100Combined sources8
Beta strandi102 – 104Combined sources3
Beta strandi106 – 108Combined sources3
Beta strandi120 – 124Combined sources5
Beta strandi125 – 135Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AY9X-ray3.00A/B32-139[»]
1I4VNMR-A/B26-139[»]
1UMUX-ray2.50A/B25-137[»]
DisProtiDP00626.
ProteinModelPortaliP0AG11.
SMRiP0AG11.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AG11.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S24 family.Curated

Phylogenomic databases

eggNOGiENOG4105KT5. Bacteria.
COG1974. LUCA.
HOGENOMiHOG000232166.
InParanoidiP0AG11.
KOiK03503.
PhylomeDBiP0AG11.

Family and domain databases

InterProiView protein in InterPro
IPR006197. Peptidase_S24_LexA.
IPR015927. Peptidase_S24_S26A/B/C.
PfamiView protein in Pfam
PF00717. Peptidase_S24. 1 hit.
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AG11-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFIKPADLR EIVTFPLFSD LVQCGFPSPA ADYVEQRIDL NQLLIQHPSA
60 70 80 90 100
TYFVKASGDS MIDGGISDGD LLIVDSAITA SHGDIVIAAV DGEFTVKKLQ
110 120 130
LRPTVQLIPM NSAYSPITIS SEDTLDVFGV VIHVVKAMR
Length:139
Mass (Da):15,063
Last modified:November 1, 1986 - v1
Checksum:i0681A3FFAC7ED583
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10107 Genomic DNA. Translation: AAA24728.1.
M13387 Genomic DNA. Translation: AAA98073.1.
U00096 Genomic DNA. Translation: AAC74267.1.
AP009048 Genomic DNA. Translation: BAA36030.1.
PIRiA03551. ZWECD.
RefSeqiNP_415701.1. NC_000913.3.
WP_000897378.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74267; AAC74267; b1183.
BAA36030; BAA36030; BAA36030.
GeneIDi945746.
KEGGiecj:JW1172.
eco:b1183.
PATRICifig|1411691.4.peg.1104.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiUMUD_ECOLI
AccessioniPrimary (citable) accession number: P0AG11
Secondary accession number(s): P04153
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: July 5, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families