Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P0AG11 (UMUD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein UmuD

EC=3.4.21.-

Cleaved into the following chain:

  1. Protein UmuD'
Gene names
Name:umuD
Ordered Locus Names:b1183, JW1172
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length139 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template.

Sequence similarities

Belongs to the peptidase S24 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 139139Protein UmuD
PRO_0000041988
Chain25 – 139115Protein UmuD'
PRO_0000027305

Sites

Active site601For autocatalytic cleavage activity
Active site971For autocatalytic cleavage activity
Site24 – 252Cleavage; by autolysis

Experimental info

Mutagenesis271P → D in umuD1; non-cleavable.
Mutagenesis651G → R in umuD44; non-cleavable.
Mutagenesis921G → D in umuD77; non-cleavable.

Secondary structure

........................ 139
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AG11 [UniParc].

Last modified November 1, 1986. Version 1.
Checksum: 0681A3FFAC7ED583

FASTA13915,063
        10         20         30         40         50         60 
MLFIKPADLR EIVTFPLFSD LVQCGFPSPA ADYVEQRIDL NQLLIQHPSA TYFVKASGDS 

        70         80         90        100        110        120 
MIDGGISDGD LLIVDSAITA SHGDIVIAAV DGEFTVKKLQ LRPTVQLIPM NSAYSPITIS 

       130 
SEDTLDVFGV VIHVVKAMR 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli."
Kitagawa Y., Akaboshi E., Shinagawa H., Horii T., Ogawa H., Kato T.
Proc. Natl. Acad. Sci. U.S.A. 82:4336-4340(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology."
Perry K.L., Elledge S.J., Mitchell B.B., Marsh L., Walker G.C.
Proc. Natl. Acad. Sci. U.S.A. 82:4331-4335(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Escherichia coli umuDC mutants: DNA sequence alterations and UmuD cleavage."
Koch W.H., Ennis D.G., Levine A.S., Woodgate R.
Mol. Gen. Genet. 233:443-448(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[7]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[8]"Structure of the UmuD' protein and its regulation in response to DNA damage."
Peat T.S., Frank E., McDonald J.P., Levine A.S., Woodgate R., Hendrickson W.A.
Nature 380:727-730(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF UMUD'.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M10107 Genomic DNA. Translation: AAA24728.1.
M13387 Genomic DNA. Translation: AAA98073.1.
U00096 Genomic DNA. Translation: AAC74267.1.
AP009048 Genomic DNA. Translation: BAA36030.1.
PIRZWECD. A03551.
RefSeqNP_415701.1. NC_000913.3.
YP_489450.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AY9X-ray3.00A/B32-139[»]
1I4VNMR-A/B26-139[»]
1UMUX-ray2.50A/B25-137[»]
DisProtDP00626.
ProteinModelPortalP0AG11.
SMRP0AG11. Positions 32-139.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-29679N.
IntActP0AG11. 13 interactions.
STRING511145.b1183.

Protein family/group databases

MEROPSS24.003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74267; AAC74267; b1183.
BAA36030; BAA36030; BAA36030.
GeneID12932823.
945746.
KEGGecj:Y75_p1155.
eco:b1183.
PATRIC32117612. VBIEscCol129921_1228.

Organism-specific databases

EchoBASEEB1050.
EcoGeneEG11057. umuD.

Phylogenomic databases

eggNOGCOG1974.
HOGENOMHOG000232166.
KOK03503.
OMAFMERVSA.
OrthoDBEOG6JHRHJ.
PhylomeDBP0AG11.

Enzyme and pathway databases

BioCycEcoCyc:EG11057-MONOMER.
ECOL316407:JW1172-MONOMER.
MetaCyc:EG11057-MONOMER.

Gene expression databases

GenevestigatorP0AG11.

Family and domain databases

Gene3D2.10.109.10. 1 hit.
InterProIPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PfamPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSPR00726. LEXASERPTASE.
SUPFAMSSF51306. SSF51306. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0AG11.
PROP0AG11.

Entry information

Entry nameUMUD_ECOLI
AccessionPrimary (citable) accession number: P0AG11
Secondary accession number(s): P04153
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene