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Protein

Ribulose-phosphate 3-epimerase

Gene

rpe

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.1 Publication

Catalytic activityi

D-ribulose 5-phosphate = D-xylulose 5-phosphate.1 Publication

Cofactori

Co2+1 Publication, Fe2+1 Publication, Mn2+1 Publication, Zn2+1 PublicationNote: Binds 1 divalent metal cation per subunit. Active with Co2+, Fe2+, Mn2+ and Zn2+.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9SubstrateBy similarity1
Metal bindingi34Divalent metal cationBy similarity1
Active sitei36Proton acceptorBy similarity1
Metal bindingi36Divalent metal cationBy similarity1
Metal bindingi68Divalent metal cationBy similarity1
Binding sitei68SubstrateBy similarity1
Active sitei177Proton donorBy similarity1
Metal bindingi177Divalent metal cationBy similarity1
Binding sitei179Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

  • ferrous iron binding Source: EcoCyc
  • ribulose-phosphate 3-epimerase activity Source: EcoCyc

GO - Biological processi

  • cellular carbohydrate metabolic process Source: GO_Central
  • pentose catabolic process Source: EcoCyc
  • pentose-phosphate shunt Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Cobalt, Iron, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:RIBULP3EPIM-MONOMER.
ECOL316407:JW3349-MONOMER.
MetaCyc:RIBULP3EPIM-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose-phosphate 3-epimerase (EC:5.1.3.1)
Alternative name(s):
Pentose-5-phosphate 3-epimerase
Short name:
PPE
R5P3E
Gene namesi
Name:rpe
Synonyms:dod, yhfD
Ordered Locus Names:b3386, JW3349
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11960. rpe.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001715681 – 225Ribulose-phosphate 3-epimeraseAdd BLAST225

Proteomic databases

EPDiP0AG07.
PaxDbiP0AG07.
PRIDEiP0AG07.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaNP0A9883EBI-546020,EBI-542385
frlDP455434EBI-546020,EBI-562037

Protein-protein interaction databases

BioGridi4259295. 150 interactors.
DIPiDIP-47869N.
IntActiP0AG07. 24 interactors.
MINTiMINT-1228320.
STRINGi511145.b3386.

Structurei

3D structure databases

ProteinModelPortaliP0AG07.
SMRiP0AG07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni144 – 147Substrate bindingBy similarity4
Regioni199 – 200Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DJV. Bacteria.
COG0036. LUCA.
HOGENOMiHOG000259347.
InParanoidiP0AG07.
KOiK01783.
OMAiHYEACTH.
PhylomeDBiP0AG07.

Family and domain databases

CDDicd00429. RPE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR026019. Ribul_P_3_epim.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
PIRSFiPIRSF001461. RPE. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AG07-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLIAPSI LSADFARLGE DTAKALAAGA DVVHFDVMDN HYVPNLTIGP
60 70 80 90 100
MVLKSLRNYG ITAPIDVHLM VKPVDRIVPD FAAAGASIIT FHPEASEHVD
110 120 130 140 150
RTLQLIKENG CKAGLVFNPA TPLSYLDYVM DKLDVILLMS VNPGFGGQSF
160 170 180 190 200
IPQTLDKLRE VRRRIDESGF DIRLEVDGGV KVNNIGEIAA AGADMFVAGS
210 220
AIFDQPDYKK VIDEMRSELA KVSHE
Length:225
Mass (Da):24,554
Last modified:December 20, 2005 - v1
Checksum:iDD678085698466FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203 – 225FDQPD…KVSHE → LTSQTTKKSTMKCAVNWQR in CAA79663 (PubMed:7603433).CuratedAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19601 Genomic DNA. Translation: CAA79663.1.
U18997 Genomic DNA. Translation: AAA58183.1.
U00096 Genomic DNA. Translation: AAC76411.1.
AP009048 Genomic DNA. Translation: BAE77905.1.
PIRiE65133.
RefSeqiNP_417845.1. NC_000913.3.
WP_000816280.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76411; AAC76411; b3386.
BAE77905; BAE77905; BAE77905.
GeneIDi947896.
KEGGiecj:JW3349.
eco:b3386.
PATRICi32122204. VBIEscCol129921_3479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19601 Genomic DNA. Translation: CAA79663.1.
U18997 Genomic DNA. Translation: AAA58183.1.
U00096 Genomic DNA. Translation: AAC76411.1.
AP009048 Genomic DNA. Translation: BAE77905.1.
PIRiE65133.
RefSeqiNP_417845.1. NC_000913.3.
WP_000816280.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AG07.
SMRiP0AG07.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259295. 150 interactors.
DIPiDIP-47869N.
IntActiP0AG07. 24 interactors.
MINTiMINT-1228320.
STRINGi511145.b3386.

Proteomic databases

EPDiP0AG07.
PaxDbiP0AG07.
PRIDEiP0AG07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76411; AAC76411; b3386.
BAE77905; BAE77905; BAE77905.
GeneIDi947896.
KEGGiecj:JW3349.
eco:b3386.
PATRICi32122204. VBIEscCol129921_3479.

Organism-specific databases

EchoBASEiEB1903.
EcoGeneiEG11960. rpe.

Phylogenomic databases

eggNOGiENOG4105DJV. Bacteria.
COG0036. LUCA.
HOGENOMiHOG000259347.
InParanoidiP0AG07.
KOiK01783.
OMAiHYEACTH.
PhylomeDBiP0AG07.

Enzyme and pathway databases

BioCyciEcoCyc:RIBULP3EPIM-MONOMER.
ECOL316407:JW3349-MONOMER.
MetaCyc:RIBULP3EPIM-MONOMER.

Miscellaneous databases

PROiP0AG07.

Family and domain databases

CDDicd00429. RPE. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR026019. Ribul_P_3_epim.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
PIRSFiPIRSF001461. RPE. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPE_ECOLI
AccessioniPrimary (citable) accession number: P0AG07
Secondary accession number(s): P32661, Q2M751
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.