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P0AG03

- UBIX_ECOLI

UniProt

P0AG03 - UBIX_ECOLI

Protein

3-octaprenyl-4-hydroxybenzoate carboxy-lyase partner protein

Gene

ubiX

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Acts in concert with UbiD to perform the decarboxylation of 4-hydroxy-3-octaprenyl-benzoate, a step in the biosynthesis of coenzyme Q. Nevertheless, it is not clear how UbiX and UbiD act together and if UbiX has itself a decarboxylase activity. UbiX may function to deliver the substrate to its UbiD partner, which performs the decarboxylation step.2 Publications

    Cofactori

    Binds 1 FMN per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei37 – 371FMNBy similarity
    Binding sitei123 – 1231FMNBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 123FMNBy similarity
    Nucleotide bindingi88 – 914FMNBy similarity

    GO - Molecular functioni

    1. 3-octaprenyl-4-hydroxybenzoate carboxy-lyase activity Source: EcoCyc

    GO - Biological processi

    1. ubiquinone biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Ubiquinone biosynthesis

    Keywords - Ligandi

    Flavoprotein, FMN

    Enzyme and pathway databases

    BioCyciEcoCyc:UBIX-MONOMER.
    ECOL316407:JW2308-MONOMER.
    MetaCyc:UBIX-MONOMER.
    UniPathwayiUPA00232.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-octaprenyl-4-hydroxybenzoate carboxy-lyase partner protein (EC:4.1.1.-)
    Alternative name(s):
    Polyprenyl p-hydroxybenzoate decarboxylase
    Gene namesi
    Name:ubiX
    Synonyms:dedF
    Ordered Locus Names:b2311, JW2308
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11044. ubiX.

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene produce very low levels of coenzyme Q8 during logarithmic growth, grow slowly on succinate as the sole carbon source, accumulate 4-hydroxy-3-octaprenyl-benzoate, and have reduced UbiG O-methyltransferase activity. In contrast, they synthesize near normal levels of Q8 in the stationary phase.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 1891893-octaprenyl-4-hydroxybenzoate carboxy-lyase partner proteinPRO_0000134950Add
    BLAST

    Proteomic databases

    PaxDbiP0AG03.

    Expressioni

    Inductioni

    During aerobic growth, expression depends on the carbon source, with the highest expression on succinate, a median expression on glycerol, and the lowest on glucose. During anaerobic growth, glucose does not inhibit expression.1 Publication

    Gene expression databases

    GenevestigatoriP0AG03.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-47873N.
    IntActiP0AG03. 6 interactions.
    MINTiMINT-1239757.
    STRINGi511145.b2311.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AG03.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0163.
    HOGENOMiHOG000225437.
    KOiK03186.
    OMAiACPGFYS.
    OrthoDBiEOG6C8MXR.
    PhylomeDBiP0AG03.

    Family and domain databases

    Gene3Di3.40.50.1950. 1 hit.
    InterProiIPR003382. Flavoprotein.
    IPR004507. UbiX_Pad1.
    [Graphical view]
    PfamiPF02441. Flavoprotein. 1 hit.
    [Graphical view]
    SUPFAMiSSF52507. SSF52507. 1 hit.
    TIGRFAMsiTIGR00421. ubiX_pad. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AG03-1 [UniParc]FASTAAdd to Basket

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    MKRLIVGISG ASGAIYGVRL LQVLRDVTDI ETHLVMSQAA RQTLSLETDF    50
    SLREVQALAD VTHDARDIAA SISSGSFQTL GMVILPCSIK TLSGIVHSYT 100
    DGLLTRAADV VLKERRPLVL CVRETPLHLG HLRLMTQAAE IGAVIMPPVP 150
    AFYHRPQSLD DVINQTVNRV LDQFAITLPE DLFARWQGA 189
    Length:189
    Mass (Da):20,695
    Last modified:December 20, 2005 - v1
    Checksum:i057255DB020BF5B4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti62 – 621T → S in AAA23970. (PubMed:3040734)Curated
    Sequence conflicti139 – 14810AEIGAVIMPP → GRNRCGDYAS in AAA23970. (PubMed:3040734)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M68935 Genomic DNA. Translation: AAA23970.1.
    U00096 Genomic DNA. Translation: AAC75371.1.
    AP009048 Genomic DNA. Translation: BAA16157.1.
    PIRiE65003. XMECFD.
    RefSeqiNP_416814.1. NC_000913.3.
    YP_490553.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75371; AAC75371; b2311.
    BAA16157; BAA16157; BAA16157.
    GeneIDi12931532.
    949033.
    KEGGiecj:Y75_p2277.
    eco:b2311.
    PATRICi32119993. VBIEscCol129921_2406.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M68935 Genomic DNA. Translation: AAA23970.1 .
    U00096 Genomic DNA. Translation: AAC75371.1 .
    AP009048 Genomic DNA. Translation: BAA16157.1 .
    PIRi E65003. XMECFD.
    RefSeqi NP_416814.1. NC_000913.3.
    YP_490553.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AG03.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47873N.
    IntActi P0AG03. 6 interactions.
    MINTi MINT-1239757.
    STRINGi 511145.b2311.

    Proteomic databases

    PaxDbi P0AG03.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75371 ; AAC75371 ; b2311 .
    BAA16157 ; BAA16157 ; BAA16157 .
    GeneIDi 12931532.
    949033.
    KEGGi ecj:Y75_p2277.
    eco:b2311.
    PATRICi 32119993. VBIEscCol129921_2406.

    Organism-specific databases

    EchoBASEi EB1037.
    EcoGenei EG11044. ubiX.

    Phylogenomic databases

    eggNOGi COG0163.
    HOGENOMi HOG000225437.
    KOi K03186.
    OMAi ACPGFYS.
    OrthoDBi EOG6C8MXR.
    PhylomeDBi P0AG03.

    Enzyme and pathway databases

    UniPathwayi UPA00232 .
    BioCyci EcoCyc:UBIX-MONOMER.
    ECOL316407:JW2308-MONOMER.
    MetaCyc:UBIX-MONOMER.

    Miscellaneous databases

    PROi P0AG03.

    Gene expression databases

    Genevestigatori P0AG03.

    Family and domain databases

    Gene3Di 3.40.50.1950. 1 hit.
    InterProi IPR003382. Flavoprotein.
    IPR004507. UbiX_Pad1.
    [Graphical view ]
    Pfami PF02441. Flavoprotein. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52507. SSF52507. 1 hit.
    TIGRFAMsi TIGR00421. ubiX_pad. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons."
      Nonet M.L., Marvel C.C., Tolan D.R.
      J. Biol. Chem. 262:12209-12217(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Regulation of the isofunctional genes ubiD and ubiX of the ubiquinone biosynthetic pathway of Escherichia coli."
      Zhang H., Javor G.T.
      FEMS Microbiol. Lett. 223:67-72(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    6. "Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis."
      Gulmezian M., Zhang H., Javor G.T., Clarke C.F.
      J. Bacteriol. 188:6435-6439(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "The role of UbiX in Escherichia coli coenzyme Q biosynthesis."
      Gulmezian M., Hyman K.R., Marbois B.N., Clarke C.F., Javor G.T.
      Arch. Biochem. Biophys. 467:144-153(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

    Entry informationi

    Entry nameiUBIX_ECOLI
    AccessioniPrimary (citable) accession number: P0AG03
    Secondary accession number(s): P09550, P77715
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3