P0AG03 (UBIX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-octaprenyl-4-hydroxybenzoate carboxy-lyase
EC=4.1.1.-

Alternative name(s):
Polyprenyl p-hydroxybenzoate decarboxylase

Gene names

Name:	ubiX
Synonyms:	dedF
Ordered Locus Names:	b2311, JW2308

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	Binds 1 FMN per subunit By similarity.
Sequence similarities	Belongs to the polyprenyl p-hydroxybenzoate / phenylacrylic acid decarboxylases family.

Ontologies

Keywords
Ligand	FMN
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquinone biosynthetic process Inferred from genetic interaction PubMed 16923914. Source: EcoCyc
Molecular_function	3-octaprenyl-4-hydroxybenzoate carboxy-lyase activity Inferred from genetic interaction PubMed 16923914. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	3-octaprenyl-4-hydroxybenzoate carboxy-lyase		PRO_0000134950
Regions
	Nucleotide binding	10 – 12	3	FMN By similarity
	Nucleotide binding	88 – 91	4	FMN By similarity
Sites
	Binding site	37	1	FMN By similarity
	Binding site	123	1	FMN By similarity
Experimental info
	Sequence conflict	62	1	T → S in AAA23970. Ref.1
	Sequence conflict	139 – 148	10	AEIGAVIMPP → GRNRCGDYAS in AAA23970. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0AG03 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 057255DB020BF5B4	FASTA	189	20,695
	10 20 30 40 50 60 MKRLIVGISG ASGAIYGVRL LQVLRDVTDI ETHLVMSQAA RQTLSLETDF SLREVQALAD 70 80 90 100 110 120 VTHDARDIAA SISSGSFQTL GMVILPCSIK TLSGIVHSYT DGLLTRAADV VLKERRPLVL 130 140 150 160 170 180 CVRETPLHLG HLRLMTQAAE IGAVIMPPVP AFYHRPQSLD DVINQTVNRV LDQFAITLPE DLFARWQGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons." Nonet M.L., Marvel C.C., Tolan D.R. J. Biol. Chem. 262:12209-12217(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M68935 Genomic DNA. Translation: AAA23970.1. U00096 Genomic DNA. Translation: AAC75371.1. AP009048 Genomic DNA. Translation: BAA16157.1.
PIR	XMECFD. E65003.
RefSeq	NP_416814.1. NC_000913.2. YP_490553.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AG03.
SMR	P0AG03. Positions 3-176.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47873N.
IntAct	P0AG03. 6 interactions.
MINT	MINT-1239757.
STRING	511145.b2311.
Proteomic databases
PaxDb	P0AG03.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75371; AAC75371; b2311. BAA16157; BAA16157; BAA16157.
GeneID	12931532. 949033.
KEGG	ecj:Y75_p2277. eco:b2311.
PATRIC	32119993. VBIEscCol129921_2406.
Organism-specific databases
EchoBASE	EB1037.
EcoGene	EG11044. ubiX.
Phylogenomic databases
eggNOG	COG0163.
HOGENOM	HOG000225437.
KO	K03186.
OMA	YENDDFF.
ProtClustDB	PRK06029.
Enzyme and pathway databases
BioCyc	EcoCyc:UBIX-MONOMER. ECOL316407:JW2308-MONOMER. MetaCyc:UBIX-MONOMER.
Gene expression databases
Genevestigator	P0AG03.
Family and domain databases
Gene3D	3.40.50.1950. 1 hit.
InterPro	IPR003382. Flavoprotein. IPR004507. PAD1. [Graphical view]
Pfam	PF02441. Flavoprotein. 1 hit. [Graphical view]
SUPFAM	SSF52507. Flavoprotein. 1 hit.
TIGRFAMs	TIGR00421. ubiX_pad. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UBIX_ECOLI

Accession

Primary (citable) accession number: P0AG03
Secondary accession number(s): P09550, P77715

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program