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Protein

Flavin prenyltransferase UbiX

Gene

ubiX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN (By similarity). Acts in concert with UbiD to perform the decarboxylation of 4-hydroxy-3-octaprenyl-benzoate, a step in the biosynthesis of coenzyme Q (PubMed:16923914, PubMed:17889824).UniRule annotation2 Publications

Catalytic activityi

Dimethylallyl phosphate + FMNH2 = prenylated FMNH2 + phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37FMNUniRule annotation1
Binding sitei123FMNUniRule annotation1
Binding sitei153DMAPUniRule annotation1
Binding sitei169DMAPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 12FMNUniRule annotation3
Nucleotide bindingi88 – 91FMNUniRule annotation4

GO - Molecular functioni

GO - Biological processi

  • coenzyme biosynthetic process Source: EcoCyc
  • ubiquinone biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionPrenyltransferase, Transferase
LigandFlavoprotein, FMN

Enzyme and pathway databases

BioCyciEcoCyc:UBIX-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavin prenyltransferase UbiXUniRule annotation (EC:2.5.1.129UniRule annotation)
Gene namesi
Name:ubiXUniRule annotation
Synonyms:dedF
Ordered Locus Names:b2311, JW2308
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11044. ubiX.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene produce very low levels of coenzyme Q8 during logarithmic growth, grow slowly on succinate as the sole carbon source, accumulate 4-hydroxy-3-octaprenyl-benzoate, and have reduced UbiG O-methyltransferase activity. In contrast, they synthesize near normal levels of Q8 in the stationary phase.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001349501 – 189Flavin prenyltransferase UbiXAdd BLAST189

Proteomic databases

PaxDbiP0AG03.
PRIDEiP0AG03.

Expressioni

Inductioni

During aerobic growth, expression depends on the carbon source, with the highest expression on succinate, a median expression on glycerol, and the lowest on glucose. During anaerobic growth, glucose does not inhibit expression.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260526. 348 interactors.
DIPiDIP-47873N.
IntActiP0AG03. 6 interactors.
MINTiMINT-1239757.
STRINGi316385.ECDH10B_2473.

Structurei

3D structure databases

ProteinModelPortaliP0AG03.
SMRiP0AG03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UbiX/PAD1 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UMA. Bacteria.
COG0163. LUCA.
HOGENOMiHOG000225437.
InParanoidiP0AG03.
KOiK03186.
PhylomeDBiP0AG03.

Family and domain databases

Gene3Di3.40.50.1950. 1 hit.
HAMAPiMF_01984. ubiX_pad. 1 hit.
InterProiView protein in InterPro
IPR036551. Flavin_trans-like.
IPR003382. Flavoprotein.
IPR004507. UbiX_Pad1.
PANTHERiPTHR43374. PTHR43374. 1 hit.
PfamiView protein in Pfam
PF02441. Flavoprotein. 1 hit.
SUPFAMiSSF52507. SSF52507. 1 hit.
TIGRFAMsiTIGR00421. ubiX_pad. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRLIVGISG ASGAIYGVRL LQVLRDVTDI ETHLVMSQAA RQTLSLETDF
60 70 80 90 100
SLREVQALAD VTHDARDIAA SISSGSFQTL GMVILPCSIK TLSGIVHSYT
110 120 130 140 150
DGLLTRAADV VLKERRPLVL CVRETPLHLG HLRLMTQAAE IGAVIMPPVP
160 170 180
AFYHRPQSLD DVINQTVNRV LDQFAITLPE DLFARWQGA
Length:189
Mass (Da):20,695
Last modified:December 20, 2005 - v1
Checksum:i057255DB020BF5B4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62T → S in AAA23970 (PubMed:3040734).Curated1
Sequence conflicti139 – 148AEIGAVIMPP → GRNRCGDYAS in AAA23970 (PubMed:3040734).Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68935 Genomic DNA. Translation: AAA23970.1.
U00096 Genomic DNA. Translation: AAC75371.1.
AP009048 Genomic DNA. Translation: BAA16157.1.
PIRiE65003. XMECFD.
RefSeqiNP_416814.1. NC_000913.3.
WP_000825700.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75371; AAC75371; b2311.
BAA16157; BAA16157; BAA16157.
GeneIDi949033.
KEGGiecj:JW2308.
eco:b2311.
PATRICifig|511145.12.peg.2406.

Similar proteinsi

Entry informationi

Entry nameiUBIX_ECOLI
AccessioniPrimary (citable) accession number: P0AG03
Secondary accession number(s): P09550, P77715
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 25, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families