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Protein

Flavin prenyltransferase UbiX

Gene

ubiX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN (By similarity). Acts in concert with UbiD to perform the decarboxylation of 4-hydroxy-3-octaprenyl-benzoate, a step in the biosynthesis of coenzyme Q (PubMed:16923914, PubMed:17889824).UniRule annotation2 Publications

Catalytic activityi

Dimethylallyl phosphate + FMNH2 = prenylated FMNH2 + phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371FMNUniRule annotation
Binding sitei123 – 1231FMNUniRule annotation
Binding sitei153 – 1531DMAPUniRule annotation
Binding sitei169 – 1691DMAPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 123FMNUniRule annotation
Nucleotide bindingi88 – 914FMNUniRule annotation

GO - Molecular functioni

  • 3-octaprenyl-4-hydroxybenzoate carboxy-lyase activity Source: EcoCyc
  • prenyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  • coenzyme biosynthetic process Source: EcoCyc
  • ubiquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Prenyltransferase, Transferase

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciEcoCyc:UBIX-MONOMER.
ECOL316407:JW2308-MONOMER.
MetaCyc:UBIX-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavin prenyltransferase UbiXUniRule annotation (EC:2.5.1.129UniRule annotation)
Gene namesi
Name:ubiXUniRule annotation
Synonyms:dedF
Ordered Locus Names:b2311, JW2308
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11044. ubiX.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene produce very low levels of coenzyme Q8 during logarithmic growth, grow slowly on succinate as the sole carbon source, accumulate 4-hydroxy-3-octaprenyl-benzoate, and have reduced UbiG O-methyltransferase activity. In contrast, they synthesize near normal levels of Q8 in the stationary phase.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 189189Flavin prenyltransferase UbiXPRO_0000134950Add
BLAST

Proteomic databases

PaxDbiP0AG03.

Expressioni

Inductioni

During aerobic growth, expression depends on the carbon source, with the highest expression on succinate, a median expression on glycerol, and the lowest on glucose. During anaerobic growth, glucose does not inhibit expression.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260526. 348 interactions.
DIPiDIP-47873N.
IntActiP0AG03. 6 interactions.
MINTiMINT-1239757.
STRINGi511145.b2311.

Structurei

3D structure databases

ProteinModelPortaliP0AG03.
SMRiP0AG03. Positions 3-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UbiX/PAD1 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UMA. Bacteria.
COG0163. LUCA.
HOGENOMiHOG000225437.
InParanoidiP0AG03.
KOiK03186.
OMAiIIMPREM.
PhylomeDBiP0AG03.

Family and domain databases

Gene3Di3.40.50.1950. 1 hit.
HAMAPiMF_01984. ubiX_pad. 1 hit.
InterProiIPR003382. Flavoprotein.
IPR004507. UbiX_Pad1.
[Graphical view]
PfamiPF02441. Flavoprotein. 1 hit.
[Graphical view]
SUPFAMiSSF52507. SSF52507. 1 hit.
TIGRFAMsiTIGR00421. ubiX_pad. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRLIVGISG ASGAIYGVRL LQVLRDVTDI ETHLVMSQAA RQTLSLETDF
60 70 80 90 100
SLREVQALAD VTHDARDIAA SISSGSFQTL GMVILPCSIK TLSGIVHSYT
110 120 130 140 150
DGLLTRAADV VLKERRPLVL CVRETPLHLG HLRLMTQAAE IGAVIMPPVP
160 170 180
AFYHRPQSLD DVINQTVNRV LDQFAITLPE DLFARWQGA
Length:189
Mass (Da):20,695
Last modified:December 20, 2005 - v1
Checksum:i057255DB020BF5B4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621T → S in AAA23970 (PubMed:3040734).Curated
Sequence conflicti139 – 14810AEIGAVIMPP → GRNRCGDYAS in AAA23970 (PubMed:3040734).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68935 Genomic DNA. Translation: AAA23970.1.
U00096 Genomic DNA. Translation: AAC75371.1.
AP009048 Genomic DNA. Translation: BAA16157.1.
PIRiE65003. XMECFD.
RefSeqiNP_416814.1. NC_000913.3.
WP_000825700.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75371; AAC75371; b2311.
BAA16157; BAA16157; BAA16157.
GeneIDi949033.
KEGGiecj:JW2308.
eco:b2311.
PATRICi32119993. VBIEscCol129921_2406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68935 Genomic DNA. Translation: AAA23970.1.
U00096 Genomic DNA. Translation: AAC75371.1.
AP009048 Genomic DNA. Translation: BAA16157.1.
PIRiE65003. XMECFD.
RefSeqiNP_416814.1. NC_000913.3.
WP_000825700.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AG03.
SMRiP0AG03. Positions 3-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260526. 348 interactions.
DIPiDIP-47873N.
IntActiP0AG03. 6 interactions.
MINTiMINT-1239757.
STRINGi511145.b2311.

Proteomic databases

PaxDbiP0AG03.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75371; AAC75371; b2311.
BAA16157; BAA16157; BAA16157.
GeneIDi949033.
KEGGiecj:JW2308.
eco:b2311.
PATRICi32119993. VBIEscCol129921_2406.

Organism-specific databases

EchoBASEiEB1037.
EcoGeneiEG11044. ubiX.

Phylogenomic databases

eggNOGiENOG4108UMA. Bacteria.
COG0163. LUCA.
HOGENOMiHOG000225437.
InParanoidiP0AG03.
KOiK03186.
OMAiIIMPREM.
PhylomeDBiP0AG03.

Enzyme and pathway databases

BioCyciEcoCyc:UBIX-MONOMER.
ECOL316407:JW2308-MONOMER.
MetaCyc:UBIX-MONOMER.

Miscellaneous databases

PROiP0AG03.

Family and domain databases

Gene3Di3.40.50.1950. 1 hit.
HAMAPiMF_01984. ubiX_pad. 1 hit.
InterProiIPR003382. Flavoprotein.
IPR004507. UbiX_Pad1.
[Graphical view]
PfamiPF02441. Flavoprotein. 1 hit.
[Graphical view]
SUPFAMiSSF52507. SSF52507. 1 hit.
TIGRFAMsiTIGR00421. ubiX_pad. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUBIX_ECOLI
AccessioniPrimary (citable) accession number: P0AG03
Secondary accession number(s): P09550, P77715
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.