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P0AG03

- UBIX_ECOLI

UniProt

P0AG03 - UBIX_ECOLI

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Protein

3-octaprenyl-4-hydroxybenzoate carboxy-lyase partner protein

Gene

ubiX

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Acts in concert with UbiD to perform the decarboxylation of 4-hydroxy-3-octaprenyl-benzoate, a step in the biosynthesis of coenzyme Q. Nevertheless, it is not clear how UbiX and UbiD act together and if UbiX has itself a decarboxylase activity. UbiX may function to deliver the substrate to its UbiD partner, which performs the decarboxylation step.2 Publications

Cofactori

Binds 1 FMN per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371FMNBy similarity
Binding sitei123 – 1231FMNBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 123FMNBy similarity
Nucleotide bindingi88 – 914FMNBy similarity

GO - Molecular functioni

  1. 3-octaprenyl-4-hydroxybenzoate carboxy-lyase activity Source: EcoCyc

GO - Biological processi

  1. ubiquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciEcoCyc:UBIX-MONOMER.
ECOL316407:JW2308-MONOMER.
MetaCyc:UBIX-MONOMER.
UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
3-octaprenyl-4-hydroxybenzoate carboxy-lyase partner protein (EC:4.1.1.-)
Alternative name(s):
Polyprenyl p-hydroxybenzoate decarboxylase
Gene namesi
Name:ubiX
Synonyms:dedF
Ordered Locus Names:b2311, JW2308
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11044. ubiX.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene produce very low levels of coenzyme Q8 during logarithmic growth, grow slowly on succinate as the sole carbon source, accumulate 4-hydroxy-3-octaprenyl-benzoate, and have reduced UbiG O-methyltransferase activity. In contrast, they synthesize near normal levels of Q8 in the stationary phase.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1891893-octaprenyl-4-hydroxybenzoate carboxy-lyase partner proteinPRO_0000134950Add
BLAST

Proteomic databases

PaxDbiP0AG03.

Expressioni

Inductioni

During aerobic growth, expression depends on the carbon source, with the highest expression on succinate, a median expression on glycerol, and the lowest on glucose. During anaerobic growth, glucose does not inhibit expression.1 Publication

Gene expression databases

GenevestigatoriP0AG03.

Interactioni

Protein-protein interaction databases

DIPiDIP-47873N.
IntActiP0AG03. 6 interactions.
MINTiMINT-1239757.
STRINGi511145.b2311.

Structurei

3D structure databases

ProteinModelPortaliP0AG03.
SMRiP0AG03. Positions 3-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0163.
HOGENOMiHOG000225437.
InParanoidiP0AG03.
KOiK03186.
OMAiACPGFYS.
OrthoDBiEOG6C8MXR.
PhylomeDBiP0AG03.

Family and domain databases

Gene3Di3.40.50.1950. 1 hit.
InterProiIPR003382. Flavoprotein.
IPR004507. UbiX_Pad1.
[Graphical view]
PfamiPF02441. Flavoprotein. 1 hit.
[Graphical view]
SUPFAMiSSF52507. SSF52507. 1 hit.
TIGRFAMsiTIGR00421. ubiX_pad. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG03-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRLIVGISG ASGAIYGVRL LQVLRDVTDI ETHLVMSQAA RQTLSLETDF
60 70 80 90 100
SLREVQALAD VTHDARDIAA SISSGSFQTL GMVILPCSIK TLSGIVHSYT
110 120 130 140 150
DGLLTRAADV VLKERRPLVL CVRETPLHLG HLRLMTQAAE IGAVIMPPVP
160 170 180
AFYHRPQSLD DVINQTVNRV LDQFAITLPE DLFARWQGA
Length:189
Mass (Da):20,695
Last modified:December 20, 2005 - v1
Checksum:i057255DB020BF5B4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621T → S in AAA23970. (PubMed:3040734)Curated
Sequence conflicti139 – 14810AEIGAVIMPP → GRNRCGDYAS in AAA23970. (PubMed:3040734)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M68935 Genomic DNA. Translation: AAA23970.1.
U00096 Genomic DNA. Translation: AAC75371.1.
AP009048 Genomic DNA. Translation: BAA16157.1.
PIRiE65003. XMECFD.
RefSeqiNP_416814.1. NC_000913.3.
YP_490553.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75371; AAC75371; b2311.
BAA16157; BAA16157; BAA16157.
GeneIDi12931532.
949033.
KEGGiecj:Y75_p2277.
eco:b2311.
PATRICi32119993. VBIEscCol129921_2406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M68935 Genomic DNA. Translation: AAA23970.1 .
U00096 Genomic DNA. Translation: AAC75371.1 .
AP009048 Genomic DNA. Translation: BAA16157.1 .
PIRi E65003. XMECFD.
RefSeqi NP_416814.1. NC_000913.3.
YP_490553.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AG03.
SMRi P0AG03. Positions 3-176.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47873N.
IntActi P0AG03. 6 interactions.
MINTi MINT-1239757.
STRINGi 511145.b2311.

Proteomic databases

PaxDbi P0AG03.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75371 ; AAC75371 ; b2311 .
BAA16157 ; BAA16157 ; BAA16157 .
GeneIDi 12931532.
949033.
KEGGi ecj:Y75_p2277.
eco:b2311.
PATRICi 32119993. VBIEscCol129921_2406.

Organism-specific databases

EchoBASEi EB1037.
EcoGenei EG11044. ubiX.

Phylogenomic databases

eggNOGi COG0163.
HOGENOMi HOG000225437.
InParanoidi P0AG03.
KOi K03186.
OMAi ACPGFYS.
OrthoDBi EOG6C8MXR.
PhylomeDBi P0AG03.

Enzyme and pathway databases

UniPathwayi UPA00232 .
BioCyci EcoCyc:UBIX-MONOMER.
ECOL316407:JW2308-MONOMER.
MetaCyc:UBIX-MONOMER.

Miscellaneous databases

PROi P0AG03.

Gene expression databases

Genevestigatori P0AG03.

Family and domain databases

Gene3Di 3.40.50.1950. 1 hit.
InterProi IPR003382. Flavoprotein.
IPR004507. UbiX_Pad1.
[Graphical view ]
Pfami PF02441. Flavoprotein. 1 hit.
[Graphical view ]
SUPFAMi SSF52507. SSF52507. 1 hit.
TIGRFAMsi TIGR00421. ubiX_pad. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons."
    Nonet M.L., Marvel C.C., Tolan D.R.
    J. Biol. Chem. 262:12209-12217(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Regulation of the isofunctional genes ubiD and ubiX of the ubiquinone biosynthetic pathway of Escherichia coli."
    Zhang H., Javor G.T.
    FEMS Microbiol. Lett. 223:67-72(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  6. "Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis."
    Gulmezian M., Zhang H., Javor G.T., Clarke C.F.
    J. Bacteriol. 188:6435-6439(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The role of UbiX in Escherichia coli coenzyme Q biosynthesis."
    Gulmezian M., Hyman K.R., Marbois B.N., Clarke C.F., Javor G.T.
    Arch. Biochem. Biophys. 467:144-153(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiUBIX_ECOLI
AccessioniPrimary (citable) accession number: P0AG03
Secondary accession number(s): P09550, P77715
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3