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Protein

ECA polysaccharide chain length modulation protein

Gene

wzzE

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Modulates the polysaccharide chain length of enterobacterial common antigen (ECA).UniRule annotation

Pathwayi: enterobacterial common antigen biosynthesis

This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

UniPathwayiUPA00566.

Names & Taxonomyi

Protein namesi
Recommended name:
ECA polysaccharide chain length modulation proteinUniRule annotation
Gene namesi
Name:wzzEUniRule annotation
Synonyms:wzz
Ordered Locus Names:Z5296, ECs4718
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicCuratedAdd BLAST30
Transmembranei31 – 51HelicalUniRule annotationAdd BLAST21
Topological domaini52 – 322PeriplasmicCuratedAdd BLAST271
Transmembranei323 – 343HelicalUniRule annotationAdd BLAST21
Topological domaini344 – 348CytoplasmicCurated5

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000659971 – 348ECA polysaccharide chain length modulation proteinAdd BLAST348

Proteomic databases

PRIDEiP0AG01.

Interactioni

Subunit structurei

Homooctamer (PubMed:18204465, PubMed:25307743). Probably part of a complex composed of WzxE, WzyE and WzzE (By similarity).UniRule annotation2 Publications

Protein-protein interaction databases

DIPiDIP-46395N.
STRINGi155864.Z5296.

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi55 – 62Combined sources8
Helixi66 – 69Combined sources4
Helixi71 – 80Combined sources10
Helixi96 – 109Combined sources14
Helixi111 – 119Combined sources9
Helixi122 – 125Combined sources4
Helixi132 – 143Combined sources12
Beta strandi146 – 149Combined sources4
Turni153 – 156Combined sources4
Beta strandi160 – 167Combined sources8
Helixi168 – 228Combined sources61
Helixi266 – 274Combined sources9
Helixi280 – 294Combined sources15
Beta strandi306 – 309Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B8OX-ray2.40A/B/C/D/E/F/G/H54-318[»]
4WL1X-ray5.99A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f2-348[»]
ProteinModelPortaliP0AG01.
SMRiP0AG01.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AG01.

Family & Domainsi

Sequence similaritiesi

Belongs to the WzzB/Cld/Rol family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105SFE. Bacteria.
COG3765. LUCA.
HOGENOMiHOG000126437.
KOiK05790.
OMAiAYDTRRE.

Family and domain databases

HAMAPiMF_02025. WzzE. 1 hit.
InterProiIPR003856. LipoPS_biosynth.
IPR032895. WzzE.
[Graphical view]
PfamiPF02706. Wzz. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AG01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQPMPGKPA EDAENELDIR GLFRTLWAGK LWIIGMGLAF ALIALAYTFF
60 70 80 90 100
ARQEWSSTAI TDRPTVNMLG GYYSQQQFLR NLDVRSNMAS ADQPSVMDEA
110 120 130 140 150
YKEFVMQLAS WDTRREFWLQ TDYYKQRMVG NSKADAALLD EMINNIQFIP
160 170 180 190 200
GDFTRAVNDS VKLIAETAPD ANNLLRQYVA FASQRAASHL NDELKGAWAA
210 220 230 240 250
RTIQMKAQVK RQEEVAKAIY DRRMNSIEQA LKIAEQHNIS RSATDVPAEE
260 270 280 290 300
LPDSEMFLLG RPMLQARLEN LQAVGPAFDL DYDQNRAMLN TLNVGPTLDP
310 320 330 340
RFQTYRYLRT PEEPVKRDSP RRAFLMIMWG IVGGLIGAGV ALTRRCSK
Length:348
Mass (Da):39,489
Last modified:December 4, 2007 - v2
Checksum:i4BE76BE122A1679F
GO

Sequence cautioni

The sequence AAG58980 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB38141 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58980.1. Different initiation.
BA000007 Genomic DNA. Translation: BAB38141.1. Different initiation.
PIRiF91218.
H86064.
RefSeqiNP_312745.1. NC_002695.1.
WP_001295256.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG58980; AAG58980; Z5296.
BAB38141; BAB38141; BAB38141.
GeneIDi915243.
KEGGiece:Z5296.
ecs:ECs4718.
PATRICi18359043. VBIEscCol44059_4680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58980.1. Different initiation.
BA000007 Genomic DNA. Translation: BAB38141.1. Different initiation.
PIRiF91218.
H86064.
RefSeqiNP_312745.1. NC_002695.1.
WP_001295256.1. NZ_LPWC02000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B8OX-ray2.40A/B/C/D/E/F/G/H54-318[»]
4WL1X-ray5.99A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f2-348[»]
ProteinModelPortaliP0AG01.
SMRiP0AG01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46395N.
STRINGi155864.Z5296.

Proteomic databases

PRIDEiP0AG01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58980; AAG58980; Z5296.
BAB38141; BAB38141; BAB38141.
GeneIDi915243.
KEGGiece:Z5296.
ecs:ECs4718.
PATRICi18359043. VBIEscCol44059_4680.

Phylogenomic databases

eggNOGiENOG4105SFE. Bacteria.
COG3765. LUCA.
HOGENOMiHOG000126437.
KOiK05790.
OMAiAYDTRRE.

Enzyme and pathway databases

UniPathwayiUPA00566.

Miscellaneous databases

EvolutionaryTraceiP0AG01.

Family and domain databases

HAMAPiMF_02025. WzzE. 1 hit.
InterProiIPR003856. LipoPS_biosynth.
IPR032895. WzzE.
[Graphical view]
PfamiPF02706. Wzz. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWZZE_ECO57
AccessioniPrimary (citable) accession number: P0AG01
Secondary accession number(s): P25905, P76752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.