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Protein

ECA polysaccharide chain length modulation protein

Gene

wzzE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Modulates the polysaccharide chain length of enterobacterial common antigen (ECA). Required for the assembly of the phosphoglyceride-linked form of ECA (ECA(PG)) and the water-soluble cyclic form of ECA (ECA(CYC)).2 Publications

Pathwayi: enterobacterial common antigen biosynthesis

This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation2 Publications
View all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG11295-MONOMER.
ECOL316407:JW5601-MONOMER.
MetaCyc:EG11295-MONOMER.
UniPathwayiUPA00566.

Names & Taxonomyi

Protein namesi
Recommended name:
ECA polysaccharide chain length modulation proteinUniRule annotationCurated
Gene namesi
Name:wzzEUniRule annotation
Synonyms:wzz1 Publication, yifC
Ordered Locus Names:b3785, JW5601
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11295. wzzE.

Subcellular locationi

  • Cell inner membrane UniRule annotationCurated; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicCuratedAdd BLAST30
Transmembranei31 – 51HelicalUniRule annotationAdd BLAST21
Topological domaini52 – 322PeriplasmicCuratedAdd BLAST271
Transmembranei323 – 343HelicalUniRule annotationAdd BLAST21
Topological domaini344 – 348Cytoplasmic1 Publication5

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Null mutation abolishes ECA(CYC) synthesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000659961 – 348ECA polysaccharide chain length modulation proteinAdd BLAST348

Proteomic databases

EPDiP0AG00.
PaxDbiP0AG00.
PRIDEiP0AG00.

Interactioni

Subunit structurei

Probably part of a complex composed of WzxE, WzyE and WzzE.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4259698. 160 interactors.
STRINGi511145.b3785.

Structurei

3D structure databases

ProteinModelPortaliP0AG00.
SMRiP0AG00.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the WzzB/Cld/Rol family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105SFE. Bacteria.
COG3765. LUCA.
HOGENOMiHOG000126437.
InParanoidiP0AG00.
KOiK05790.
OMAiAYDTRRE.
PhylomeDBiP0AG00.

Family and domain databases

HAMAPiMF_02025. WzzE. 1 hit.
InterProiIPR003856. LipoPS_biosynth.
IPR032895. WzzE.
[Graphical view]
PfamiPF02706. Wzz. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AG00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQPMPGKPA EDAENELDIR GLFRTLWAGK LWIIGMGLAF ALIALAYTFF
60 70 80 90 100
ARQEWSSTAI TDRPTVNMLG GYYSQQQFLR NLDVRSNMAS ADQPSVMDEA
110 120 130 140 150
YKEFVMQLAS WDTRREFWLQ TDYYKQRMVG NSKADAALLD EMINNIQFIP
160 170 180 190 200
GDFTRAVNDS VKLIAETAPD ANNLLRQYVA FASQRAASHL NDELKGAWAA
210 220 230 240 250
RTIQMKAQVK RQEEVAKAIY DRRMNSIEQA LKIAEQHNIS RSATDVPAEE
260 270 280 290 300
LPDSEMFLLG RPMLQARLEN LQAVGPAFDL DYDQNRAMLN TLNVGPTLDP
310 320 330 340
RFQTYRYLRT PEEPVKRDSP RRAFLMIMWG IVGGLIGAGV ALTRRCSK
Length:348
Mass (Da):39,489
Last modified:December 4, 2007 - v2
Checksum:i4BE76BE122A1679F
GO

Sequence cautioni

The sequence AAA67585 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67585.1. Different initiation.
M76129 Genomic DNA. Translation: AAA24527.1.
U00096 Genomic DNA. Translation: AAC76790.2.
AP009048 Genomic DNA. Translation: BAE77513.1.
PIRiD65182.
RefSeqiNP_418232.2. NC_000913.3.
WP_001295256.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76790; AAC76790; b3785.
BAE77513; BAE77513; BAE77513.
GeneIDi944815.
KEGGiecj:JW5601.
eco:b3785.
PATRICi32123063. VBIEscCol129921_3901.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67585.1. Different initiation.
M76129 Genomic DNA. Translation: AAA24527.1.
U00096 Genomic DNA. Translation: AAC76790.2.
AP009048 Genomic DNA. Translation: BAE77513.1.
PIRiD65182.
RefSeqiNP_418232.2. NC_000913.3.
WP_001295256.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AG00.
SMRiP0AG00.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259698. 160 interactors.
STRINGi511145.b3785.

Proteomic databases

EPDiP0AG00.
PaxDbiP0AG00.
PRIDEiP0AG00.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76790; AAC76790; b3785.
BAE77513; BAE77513; BAE77513.
GeneIDi944815.
KEGGiecj:JW5601.
eco:b3785.
PATRICi32123063. VBIEscCol129921_3901.

Organism-specific databases

EchoBASEiEB1272.
EcoGeneiEG11295. wzzE.

Phylogenomic databases

eggNOGiENOG4105SFE. Bacteria.
COG3765. LUCA.
HOGENOMiHOG000126437.
InParanoidiP0AG00.
KOiK05790.
OMAiAYDTRRE.
PhylomeDBiP0AG00.

Enzyme and pathway databases

UniPathwayiUPA00566.
BioCyciEcoCyc:EG11295-MONOMER.
ECOL316407:JW5601-MONOMER.
MetaCyc:EG11295-MONOMER.

Miscellaneous databases

PROiP0AG00.

Family and domain databases

HAMAPiMF_02025. WzzE. 1 hit.
InterProiIPR003856. LipoPS_biosynth.
IPR032895. WzzE.
[Graphical view]
PfamiPF02706. Wzz. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWZZE_ECOLI
AccessioniPrimary (citable) accession number: P0AG00
Secondary accession number(s): P25905, P76752, Q2M893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.