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P0AFZ7

- TRKH_ECOLI

UniProt

P0AFZ7 - TRKH_ECOLI

Protein

Trk system potassium uptake protein TrkH

Gene

trkH

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Low-affinity potassium transport system. Interacts with Trk system potassium uptake protein TrkA and requires TrkE for transport activity By similarity. Can transport potassium and rubidium.By similarity1 Publication

    Kineticsi

    1. KM=6 mM for K+1 Publication
    2. KM=2 mM for Rb+1 Publication

    Vmax=800 µmol/min/g enzyme with K+ as substrate1 Publication

    Vmax=10 µmol/min/g enzyme with Rb+ as substrate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei111 – 1111Potassium ion; via carbonyl oxygenBy similarity
    Binding sitei112 – 1121Potassium ion; via carbonyl oxygenBy similarity
    Binding sitei221 – 2211Potassium ion; via carbonyl oxygenBy similarity
    Binding sitei435 – 4351Potassium ion; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. potassium channel activity Source: UniProtKB
    2. potassium ion binding Source: UniProtKB
    3. potassium ion symporter activity Source: InterPro
    4. potassium uptake transmembrane transporter activity Source: EcoCyc

    GO - Biological processi

    1. potassium ion transmembrane transport Source: UniProtKB
    2. potassium ion transport Source: EcoCyc

    Keywords - Molecular functioni

    Ion channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    BioCyciEcoCyc:TRKH-MONOMER.
    ECOL316407:JW5576-MONOMER.
    MetaCyc:TRKH-MONOMER.

    Protein family/group databases

    TCDBi2.A.38.1.1. the k(+) transporter (trk) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trk system potassium uptake protein TrkH
    Gene namesi
    Name:trkH
    Ordered Locus Names:b3849, JW5576
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11021. trkH.

    Subcellular locationi

    Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 483483Trk system potassium uptake protein TrkHPRO_0000070475Add
    BLAST

    Proteomic databases

    PRIDEiP0AFZ7.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AFZ7.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-47978N.
    IntActiP0AFZ7. 2 interactions.
    MINTiMINT-1238565.
    STRINGi511145.b3849.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AFZ7.
    SMRiP0AFZ7. Positions 1-482.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini? – 483CytoplasmicBy similarity
    Topological domaini? – 389CytoplasmicBy similarity
    Topological domaini? – 273CytoplasmicBy similarity
    Topological domaini1 – 22CytoplasmicBy similarity
    Topological domaini30 – 356PeriplasmicBy similarity
    Topological domaini58 – 658CytoplasmicBy similarity
    Topological domaini91 – ?PeriplasmicBy similarity
    Topological domaini116 – 1249PeriplasmicBy similarity
    Topological domaini151 – 17727CytoplasmicBy similarityAdd
    BLAST
    Topological domaini203 – 2053PeriplasmicBy similarity
    Topological domaini225 – 23410PeriplasmicBy similarity
    Topological domaini295 – ?PeriplasmicBy similarity
    Topological domaini323 – 3308PeriplasmicBy similarity
    Topological domaini418 – 4192PeriplasmicBy similarity
    Topological domaini440 – 45112PeriplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei? – 300By similarity
    Intramembranei? – 97By similarity
    Intramembranei98 – 10912Helical; Pore-formingBy similarityAdd
    BLAST
    Intramembranei110 – 1156By similarity
    Intramembranei206 – 2061By similarity
    Intramembranei207 – 21812Helical; Pore-formingBy similarityAdd
    BLAST
    Intramembranei219 – 2246By similarity
    Intramembranei235 – 25016HelicalBy similarityAdd
    BLAST
    Intramembranei251 – ?By similarity
    Intramembranei301 – 31616Helical; Pore-formingBy similarityAdd
    BLAST
    Intramembranei317 – 3226By similarity
    Intramembranei331 – 34212HelicalBy similarityAdd
    BLAST
    Intramembranei343 – 35513Note=Loop between two helicesBy similarityAdd
    BLAST
    Intramembranei356 – ?HelicalBy similarity
    Intramembranei420 – 4212By similarity
    Intramembranei422 – 43211Helical; Pore-formingBy similarityAdd
    BLAST
    Intramembranei433 – 4397By similarity
    Intramembranei452 – 46312HelicalBy similarityAdd
    BLAST
    Intramembranei464 – ?By similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei3 – 2927HelicalBy similarityAdd
    BLAST
    Transmembranei36 – 5722HelicalBy similarityAdd
    BLAST
    Transmembranei66 – 9025HelicalBy similarityAdd
    BLAST
    Transmembranei125 – 15026HelicalBy similarityAdd
    BLAST
    Transmembranei178 – 20225HelicalBy similarityAdd
    BLAST
    Transmembranei274 – 29421HelicalBy similarityAdd
    BLAST
    Transmembranei390 – 41728HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni110 – 1156Selectivity filter part 1By similarity
    Regioni219 – 2246Selectivity filter part 2By similarity
    Regioni317 – 3226Selectivity filter part 3By similarity
    Regioni434 – 4396Selectivity filter part 4By similarity

    Sequence similaritiesi

    Belongs to the TrkH potassium transport family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0168.
    HOGENOMiHOG000225541.
    KOiK03498.
    OMAiPFIFSEQ.
    OrthoDBiEOG63589N.
    PhylomeDBiP0AFZ7.

    Family and domain databases

    InterProiIPR003445. Cat_transpt.
    IPR004772. K_uptake_TrkH_fam.
    [Graphical view]
    PfamiPF02386. TrkH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006247. TrkH. 1 hit.
    TIGRFAMsiTIGR00933. 2a38. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AFZ7-1 [UniParc]FASTAAdd to Basket

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    MHFRAITRIV GLLVILFSGT MIIPGLVALI YRDGAGRAFT QTFFVALAIG    50
    SMLWWPNRKE KGELKSREGF LIVVLFWTVL GSVGALPFIF SESPNLTITD 100
    AFFESFSGLT TTGATTLVGL DSLPHAILFY RQMLQWFGGM GIIVLAVAIL 150
    PILGVGGMQL YRAEMPGPLK DNKMRPRIAE TAKTLWLIYV LLTVACALAL 200
    WFAGMDAFDA IGHSFATIAI GGFSTHDASI GYFDSPTINT IIAIFLLISG 250
    CNYGLHFSLL SGRSLKVYWR DPEFRMFIGV QFTLVVICTL VLWFHNVYSS 300
    ALMTINQAFF QVVSMATTAG FTTDSIARWP LFLPVLLLCS AFIGGCAGST 350
    GGGLKVIRIL LLFKQGNREL KRLVHPNAVY SIKLGNRALP ERILEAVWGF 400
    FSAYALVFIV SMLAIIATGV DDFSAFASVV ATLNNLGPGL GVVADNFTSM 450
    NPVAKWILIA NMLFGRLEVF TLLVLFTPTF WRE 483
    Length:483
    Mass (Da):52,960
    Last modified:December 20, 2005 - v1
    Checksum:i1AA9CC2F83EB509A
    GO

    Sequence cautioni

    The sequence AAA67646.1 differs from that shown. Reason: Frameshift at position 416.
    The sequence X54687 differs from that shown. Reason: Frameshift at positions 418 and 420.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti69 – 691Missing in AAA67646. (PubMed:1379743)Curated
    Sequence conflicti144 – 1441V → IV in X54687. (PubMed:2243799)Curated
    Sequence conflicti196 – 1961C → S in X54687. (PubMed:2243799)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54687 Genomic DNA. No translation available.
    M87049 Genomic DNA. Translation: AAA67646.1. Frameshift.
    U00096 Genomic DNA. Translation: AAT48231.1.
    AP009048 Genomic DNA. Translation: BAE77454.1.
    PIRiB65190.
    RefSeqiYP_026273.1. NC_000913.3.
    YP_491595.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAT48231; AAT48231; b3849.
    BAE77454; BAE77454; BAE77454.
    GeneIDi12933625.
    948333.
    KEGGiecj:Y75_p3331.
    eco:b3849.
    PATRICi32123195. VBIEscCol129921_3963.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54687 Genomic DNA. No translation available.
    M87049 Genomic DNA. Translation: AAA67646.1 . Frameshift.
    U00096 Genomic DNA. Translation: AAT48231.1 .
    AP009048 Genomic DNA. Translation: BAE77454.1 .
    PIRi B65190.
    RefSeqi YP_026273.1. NC_000913.3.
    YP_491595.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AFZ7.
    SMRi P0AFZ7. Positions 1-482.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47978N.
    IntActi P0AFZ7. 2 interactions.
    MINTi MINT-1238565.
    STRINGi 511145.b3849.

    Protein family/group databases

    TCDBi 2.A.38.1.1. the k(+) transporter (trk) family.

    Proteomic databases

    PRIDEi P0AFZ7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT48231 ; AAT48231 ; b3849 .
    BAE77454 ; BAE77454 ; BAE77454 .
    GeneIDi 12933625.
    948333.
    KEGGi ecj:Y75_p3331.
    eco:b3849.
    PATRICi 32123195. VBIEscCol129921_3963.

    Organism-specific databases

    EchoBASEi EB1014.
    EcoGenei EG11021. trkH.

    Phylogenomic databases

    eggNOGi COG0168.
    HOGENOMi HOG000225541.
    KOi K03498.
    OMAi PFIFSEQ.
    OrthoDBi EOG63589N.
    PhylomeDBi P0AFZ7.

    Enzyme and pathway databases

    BioCyci EcoCyc:TRKH-MONOMER.
    ECOL316407:JW5576-MONOMER.
    MetaCyc:TRKH-MONOMER.

    Miscellaneous databases

    PROi P0AFZ7.

    Gene expression databases

    Genevestigatori P0AFZ7.

    Family and domain databases

    InterProi IPR003445. Cat_transpt.
    IPR004772. K_uptake_TrkH_fam.
    [Graphical view ]
    Pfami PF02386. TrkH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006247. TrkH. 1 hit.
    TIGRFAMsi TIGR00933. 2a38. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence between the fadB gene and the rrnA operon from Escherichia coli."
      Nakahigashi K., Inokuchi H.
      Nucleic Acids Res. 18:6439-6439(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "TrkH and its homolog, TrkG, determine the specificity and kinetics of cation transport by the Trk system of Escherichia coli."
      Schlosser A., Meldorf M., Stumpe S., Bakker E.P., Epstein W.
      J. Bacteriol. 177:1908-1910(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    3. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
      Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
      Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 69.
      Strain: K12 / MG1655 / ATCC 47076.
    5. Cited for: SEQUENCE REVISION.
    6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    7. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiTRKH_ECOLI
    AccessioniPrimary (citable) accession number: P0AFZ7
    Secondary accession number(s): P21166, P76769, Q2M8F2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3