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P0AFZ7

- TRKH_ECOLI

UniProt

P0AFZ7 - TRKH_ECOLI

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Protein

Trk system potassium uptake protein TrkH

Gene

trkH

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Low-affinity potassium transport system. Interacts with Trk system potassium uptake protein TrkA and requires TrkE for transport activity (By similarity). Can transport potassium and rubidium.By similarity1 Publication

Kineticsi

  1. KM=6 mM for K+1 Publication
  2. KM=2 mM for Rb+1 Publication

Vmax=800 µmol/min/g enzyme with K+ as substrate1 Publication

Vmax=10 µmol/min/g enzyme with Rb+ as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111Potassium ion; via carbonyl oxygenBy similarity
Binding sitei112 – 1121Potassium ion; via carbonyl oxygenBy similarity
Binding sitei221 – 2211Potassium ion; via carbonyl oxygenBy similarity
Binding sitei435 – 4351Potassium ion; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. potassium channel activity Source: UniProtKB
  2. potassium ion binding Source: UniProtKB
  3. potassium ion symporter activity Source: InterPro
  4. potassium uptake transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  1. potassium ion transmembrane transport Source: UniProtKB
  2. potassium ion transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciEcoCyc:TRKH-MONOMER.
ECOL316407:JW5576-MONOMER.
MetaCyc:TRKH-MONOMER.

Protein family/group databases

TCDBi2.A.38.1.1. the k(+) transporter (trk) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Trk system potassium uptake protein TrkH
Gene namesi
Name:trkH
Ordered Locus Names:b3849, JW5576
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11021. trkH.

Subcellular locationi

Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Trk system potassium uptake protein TrkHPRO_0000070475Add
BLAST

Proteomic databases

PRIDEiP0AFZ7.

Expressioni

Gene expression databases

GenevestigatoriP0AFZ7.

Interactioni

Protein-protein interaction databases

DIPiDIP-47978N.
IntActiP0AFZ7. 2 interactions.
MINTiMINT-1238565.
STRINGi511145.b3849.

Structurei

3D structure databases

ProteinModelPortaliP0AFZ7.
SMRiP0AFZ7. Positions 1-482.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini? – 483CytoplasmicBy similarity
Topological domaini? – 389CytoplasmicBy similarity
Topological domaini? – 273CytoplasmicBy similarity
Topological domaini1 – 22CytoplasmicBy similarity
Topological domaini30 – 356PeriplasmicBy similarity
Topological domaini58 – 658CytoplasmicBy similarity
Topological domaini91 – ?PeriplasmicBy similarity
Topological domaini116 – 1249PeriplasmicBy similarity
Topological domaini151 – 17727CytoplasmicBy similarityAdd
BLAST
Topological domaini203 – 2053PeriplasmicBy similarity
Topological domaini225 – 23410PeriplasmicBy similarity
Topological domaini295 – ?PeriplasmicBy similarity
Topological domaini323 – 3308PeriplasmicBy similarity
Topological domaini418 – 4192PeriplasmicBy similarity
Topological domaini440 – 45112PeriplasmicBy similarityAdd
BLAST

Intramembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei? – 300By similarity
Intramembranei? – 97By similarity
Intramembranei98 – 10912Helical; Pore-formingBy similarityAdd
BLAST
Intramembranei110 – 1156By similarity
Intramembranei206 – 2061By similarity
Intramembranei207 – 21812Helical; Pore-formingBy similarityAdd
BLAST
Intramembranei219 – 2246By similarity
Intramembranei235 – 25016HelicalBy similarityAdd
BLAST
Intramembranei251 – ?By similarity
Intramembranei301 – 31616Helical; Pore-formingBy similarityAdd
BLAST
Intramembranei317 – 3226By similarity
Intramembranei331 – 34212HelicalBy similarityAdd
BLAST
Intramembranei343 – 35513Note=Loop between two helicesBy similarityAdd
BLAST
Intramembranei356 – ?HelicalBy similarity
Intramembranei420 – 4212By similarity
Intramembranei422 – 43211Helical; Pore-formingBy similarityAdd
BLAST
Intramembranei433 – 4397By similarity
Intramembranei452 – 46312HelicalBy similarityAdd
BLAST
Intramembranei464 – ?By similarity

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2927HelicalBy similarityAdd
BLAST
Transmembranei36 – 5722HelicalBy similarityAdd
BLAST
Transmembranei66 – 9025HelicalBy similarityAdd
BLAST
Transmembranei125 – 15026HelicalBy similarityAdd
BLAST
Transmembranei178 – 20225HelicalBy similarityAdd
BLAST
Transmembranei274 – 29421HelicalBy similarityAdd
BLAST
Transmembranei390 – 41728HelicalBy similarityAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni110 – 1156Selectivity filter part 1By similarity
Regioni219 – 2246Selectivity filter part 2By similarity
Regioni317 – 3226Selectivity filter part 3By similarity
Regioni434 – 4396Selectivity filter part 4By similarity

Sequence similaritiesi

Belongs to the TrkH potassium transport family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0168.
HOGENOMiHOG000225541.
InParanoidiP0AFZ7.
KOiK03498.
OMAiPFIFSEQ.
OrthoDBiEOG63589N.
PhylomeDBiP0AFZ7.

Family and domain databases

InterProiIPR003445. Cat_transpt.
IPR004772. K_uptake_TrkH_fam.
[Graphical view]
PfamiPF02386. TrkH. 1 hit.
[Graphical view]
PIRSFiPIRSF006247. TrkH. 1 hit.
TIGRFAMsiTIGR00933. 2a38. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFZ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHFRAITRIV GLLVILFSGT MIIPGLVALI YRDGAGRAFT QTFFVALAIG
60 70 80 90 100
SMLWWPNRKE KGELKSREGF LIVVLFWTVL GSVGALPFIF SESPNLTITD
110 120 130 140 150
AFFESFSGLT TTGATTLVGL DSLPHAILFY RQMLQWFGGM GIIVLAVAIL
160 170 180 190 200
PILGVGGMQL YRAEMPGPLK DNKMRPRIAE TAKTLWLIYV LLTVACALAL
210 220 230 240 250
WFAGMDAFDA IGHSFATIAI GGFSTHDASI GYFDSPTINT IIAIFLLISG
260 270 280 290 300
CNYGLHFSLL SGRSLKVYWR DPEFRMFIGV QFTLVVICTL VLWFHNVYSS
310 320 330 340 350
ALMTINQAFF QVVSMATTAG FTTDSIARWP LFLPVLLLCS AFIGGCAGST
360 370 380 390 400
GGGLKVIRIL LLFKQGNREL KRLVHPNAVY SIKLGNRALP ERILEAVWGF
410 420 430 440 450
FSAYALVFIV SMLAIIATGV DDFSAFASVV ATLNNLGPGL GVVADNFTSM
460 470 480
NPVAKWILIA NMLFGRLEVF TLLVLFTPTF WRE
Length:483
Mass (Da):52,960
Last modified:December 20, 2005 - v1
Checksum:i1AA9CC2F83EB509A
GO

Sequence cautioni

The sequence AAA67646.1 differs from that shown. Reason: Frameshift at position 416.
The sequence X54687 differs from that shown. Reason: Frameshift at positions 418 and 420.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 691Missing in AAA67646. (PubMed:1379743)Curated
Sequence conflicti144 – 1441V → IV in X54687. (PubMed:2243799)Curated
Sequence conflicti196 – 1961C → S in X54687. (PubMed:2243799)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54687 Genomic DNA. No translation available.
M87049 Genomic DNA. Translation: AAA67646.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48231.1.
AP009048 Genomic DNA. Translation: BAE77454.1.
PIRiB65190.
RefSeqiYP_026273.1. NC_000913.3.
YP_491595.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAT48231; AAT48231; b3849.
BAE77454; BAE77454; BAE77454.
GeneIDi12933625.
948333.
KEGGiecj:Y75_p3331.
eco:b3849.
PATRICi32123195. VBIEscCol129921_3963.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54687 Genomic DNA. No translation available.
M87049 Genomic DNA. Translation: AAA67646.1 . Frameshift.
U00096 Genomic DNA. Translation: AAT48231.1 .
AP009048 Genomic DNA. Translation: BAE77454.1 .
PIRi B65190.
RefSeqi YP_026273.1. NC_000913.3.
YP_491595.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AFZ7.
SMRi P0AFZ7. Positions 1-482.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47978N.
IntActi P0AFZ7. 2 interactions.
MINTi MINT-1238565.
STRINGi 511145.b3849.

Protein family/group databases

TCDBi 2.A.38.1.1. the k(+) transporter (trk) family.

Proteomic databases

PRIDEi P0AFZ7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT48231 ; AAT48231 ; b3849 .
BAE77454 ; BAE77454 ; BAE77454 .
GeneIDi 12933625.
948333.
KEGGi ecj:Y75_p3331.
eco:b3849.
PATRICi 32123195. VBIEscCol129921_3963.

Organism-specific databases

EchoBASEi EB1014.
EcoGenei EG11021. trkH.

Phylogenomic databases

eggNOGi COG0168.
HOGENOMi HOG000225541.
InParanoidi P0AFZ7.
KOi K03498.
OMAi PFIFSEQ.
OrthoDBi EOG63589N.
PhylomeDBi P0AFZ7.

Enzyme and pathway databases

BioCyci EcoCyc:TRKH-MONOMER.
ECOL316407:JW5576-MONOMER.
MetaCyc:TRKH-MONOMER.

Miscellaneous databases

PROi P0AFZ7.

Gene expression databases

Genevestigatori P0AFZ7.

Family and domain databases

InterProi IPR003445. Cat_transpt.
IPR004772. K_uptake_TrkH_fam.
[Graphical view ]
Pfami PF02386. TrkH. 1 hit.
[Graphical view ]
PIRSFi PIRSF006247. TrkH. 1 hit.
TIGRFAMsi TIGR00933. 2a38. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence between the fadB gene and the rrnA operon from Escherichia coli."
    Nakahigashi K., Inokuchi H.
    Nucleic Acids Res. 18:6439-6439(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "TrkH and its homolog, TrkG, determine the specificity and kinetics of cation transport by the Trk system of Escherichia coli."
    Schlosser A., Meldorf M., Stumpe S., Bakker E.P., Epstein W.
    J. Bacteriol. 177:1908-1910(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  3. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 69.
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: SEQUENCE REVISION.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiTRKH_ECOLI
AccessioniPrimary (citable) accession number: P0AFZ7
Secondary accession number(s): P21166, P76769, Q2M8F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3