Trk system potassium uptake protein TrkH - Escherichia coli (strain K12)

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P0AFZ7 (TRKH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 70. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Trk system potassium uptake protein TrkH

Gene names

Name:	trkH
Ordered Locus Names:	b3849, JW5576

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Low-affinity potassium transport system. Interacts with Trk system potassium uptake protein TrkA and requires TrkE for transport activity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.7.
Sequence similarities	Belongs to the TrkH potassium transport family.
Sequence caution	The sequence AAA67646.1 differs from that shown. Reason: Frameshift at position 416. The sequence X54687 differs from that shown. Reason: Frameshift at positions 418 and 420.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	potassium channel activity Inferred from sequence or structural similarity. Source: UniProtKB potassium ion binding Inferred from sequence or structural similarity. Source: UniProtKB potassium ion symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 483

483

Trk system potassium uptake protein TrkH

PRO_0000070475

Regions

Topological domain

1 – 2

Cytoplasmic By similarity

Transmembrane

3 – 29

Helical; By similarity

Topological domain

30 – 35

Periplasmic By similarity

Transmembrane

36 – 57

Helical; By similarity

Topological domain

58 – 65

Cytoplasmic By similarity

Transmembrane

66 – 90

Helical; By similarity

Topological domain

91 – ?

Periplasmic By similarity

Intramembrane

? – 97

By similarity

Intramembrane

98 – 109

Helical; Pore-forming; By similarity

Intramembrane

110 – 115

By similarity

Topological domain

116 – 124

Periplasmic By similarity

Transmembrane

125 – 150

Helical; By similarity

Topological domain

151 – 177

Cytoplasmic By similarity

Transmembrane

178 – 202

Helical; By similarity

Topological domain

203 – 205

Periplasmic By similarity

Intramembrane

206

By similarity

Intramembrane

207 – 218

Helical; Pore-forming; By similarity

Intramembrane

219 – 224

By similarity

Topological domain

225 – 234

Periplasmic By similarity

Intramembrane

235 – 250

Helical; By similarity

Intramembrane

251 – ?

By similarity

Topological domain

? – 273

Cytoplasmic By similarity

Transmembrane

274 – 294

Helical; By similarity

Topological domain

295 – ?

Periplasmic By similarity

Intramembrane

? – 300

By similarity

Intramembrane

301 – 316

Helical; Pore-forming; By similarity

Intramembrane

317 – 322

By similarity

Topological domain

323 – 330

Periplasmic By similarity

Intramembrane

331 – 342

Helical; By similarity

Intramembrane

343 – 355

Note=Loop between two helices; By similarity

Intramembrane

356 – ?

Helical; By similarity

Topological domain

? – 389

Cytoplasmic By similarity

Transmembrane

390 – 417

Helical; By similarity

Topological domain

418 – 419

Periplasmic By similarity

Intramembrane

420 – 421

By similarity

Intramembrane

422 – 432

Helical; Pore-forming; By similarity

Intramembrane

433 – 439

By similarity

Topological domain

440 – 451

Periplasmic By similarity

Intramembrane

452 – 463

Helical; By similarity

Intramembrane

464 – ?

By similarity

Topological domain

? – 483

Cytoplasmic By similarity

Region

110 – 115

Selectivity filter part 1 By similarity

Region

219 – 224

Selectivity filter part 2 By similarity

Region

317 – 322

Selectivity filter part 3 By similarity

Region

434 – 439

Selectivity filter part 4 By similarity

Sites

Binding site

111

Potassium ion; via carbonyl oxygen By similarity

Binding site

112

Potassium ion; via carbonyl oxygen By similarity

Binding site

221

Potassium ion; via carbonyl oxygen By similarity

Binding site

435

Potassium ion; via carbonyl oxygen By similarity

Experimental info

Sequence conflict

Missing in AAA67646. Ref.3

Sequence conflict

144

V → IV in X54687. Ref.1

Sequence conflict

196

C → S in X54687. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P0AFZ7 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 1AA9CC2F83EB509A
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FASTA

483

52,960

        10         20         30         40         50         60 
MHFRAITRIV GLLVILFSGT MIIPGLVALI YRDGAGRAFT QTFFVALAIG SMLWWPNRKE 

        70         80         90        100        110        120 
KGELKSREGF LIVVLFWTVL GSVGALPFIF SESPNLTITD AFFESFSGLT TTGATTLVGL 

       130        140        150        160        170        180 
DSLPHAILFY RQMLQWFGGM GIIVLAVAIL PILGVGGMQL YRAEMPGPLK DNKMRPRIAE 

       190        200        210        220        230        240 
TAKTLWLIYV LLTVACALAL WFAGMDAFDA IGHSFATIAI GGFSTHDASI GYFDSPTINT 

       250        260        270        280        290        300 
IIAIFLLISG CNYGLHFSLL SGRSLKVYWR DPEFRMFIGV QFTLVVICTL VLWFHNVYSS 

       310        320        330        340        350        360 
ALMTINQAFF QVVSMATTAG FTTDSIARWP LFLPVLLLCS AFIGGCAGST GGGLKVIRIL 

       370        380        390        400        410        420 
LLFKQGNREL KRLVHPNAVY SIKLGNRALP ERILEAVWGF FSAYALVFIV SMLAIIATGV 

       430        440        450        460        470        480 
DDFSAFASVV ATLNNLGPGL GVVADNFTSM NPVAKWILIA NMLFGRLEVF TLLVLFTPTF 


WRE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence between the fadB gene and the rrnA operon from Escherichia coli." Nakahigashi K., Inokuchi H. Nucleic Acids Res. 18:6439-6439(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"TrkH and its homolog, TrkG, determine the specificity and kinetics of cation transport by the Trk system of Escherichia coli." Schlosser A., Meldorf M., Stumpe S., Bakker E.P., Epstein W. J. Bacteriol. 177:1908-1910(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 69. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54687 Genomic DNA. No translation available. M87049 Genomic DNA. Translation: AAA67646.1. Frameshift. U00096 Genomic DNA. Translation: AAT48231.1. AP009048 Genomic DNA. Translation: BAE77454.1.
PIR	B65190.
RefSeq	YP_026273.1. NC_000913.2. YP_491595.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AFZ7.
SMR	P0AFZ7. Positions 1-482.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47978N.
IntAct	P0AFZ7. 2 interactions.
MINT	MINT-1238565.
STRING	511145.b3849.
Protein family/group databases
TCDB	2.A.38.1.1. K+ transporter (Trk) family.
Proteomic databases
PRIDE	P0AFZ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT48231; AAT48231; b3849. BAE77454; BAE77454; BAE77454.
GeneID	12933625. 948333.
KEGG	ecj:Y75_p3331. eco:b3849.
PATRIC	32123195. VBIEscCol129921_3963.
Organism-specific databases
EchoBASE	EB1014.
EcoGene	EG11021. trkH.
Phylogenomic databases
eggNOG	COG0168.
HOGENOM	HOG000225541.
KO	K03498.
OMA	LFTPAFW.
ProtClustDB	PRK10750.
Enzyme and pathway databases
BioCyc	EcoCyc:TRKH-MONOMER. ECOL316407:JW5576-MONOMER.
Gene expression databases
Genevestigator	P0AFZ7.
Family and domain databases
InterPro	IPR003445. Cat_transpt. IPR004772. K_uptake_TrkH_fam. [Graphical view]
Pfam	PF02386. TrkH. 1 hit. [Graphical view]
PIRSF	PIRSF006247. TrkH. 1 hit.
TIGRFAMs	TIGR00933. 2a38. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRKH_ECOLI

Accession

Primary (citable) accession number: P0AFZ7
Secondary accession number(s): P21166, P76769, Q2M8F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents