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Protein

Negative modulator of initiation of replication

Gene

seqA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of replication initiation, which contributes to regulation of DNA replication and ensures that replication initiation occurs exactly once per chromosome per cell cycle. Binds to pairs of hemimethylated GATC sequences in the oriC region, thus preventing assembly of replication proteins and re-initiation at newly replicated origins. Repression is relieved when the region becomes fully methylated. Can also bind to hemimethylated GATC sequences outside of oriC region. Binds, with less affinity, to fully methylated GATC sites and affects timing of replication. May play a role in chromosome organization and gene regulation.UniRule annotation6 Publications

GO - Molecular functioni

  • DNA replication origin binding Source: EcoCyc
  • double-stranded methylated DNA binding Source: EcoCyc
  • hemi-methylated DNA-binding Source: EcoCyc
  • identical protein binding Source: IntAct

GO - Biological processi

  • negative regulation of DNA-dependent DNA replication initiation Source: EcoCyc
  • nucleoid organization Source: EcoCyc
  • protein homotetramerization Source: EcoCyc
  • regulation of transcription, DNA-templated Source: InterPro
  • sister chromatid cohesion Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

DNA replication inhibitor

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12197-MONOMER.
ECOL316407:JW0674-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative modulator of initiation of replicationUniRule annotation
Gene namesi
Name:seqAUniRule annotation
Ordered Locus Names:b0687, JW0674
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12197. seqA.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

  • Note: Localization is cell cycle-dependent. Localizes at midcell in newborn cells, then migrates in opposite directions toward cell quarter sites and remains tethered there until the cell divides.

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • SeqA-DNA complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116R → A: Strongly reduced DNA binding. 1 Publication1
Mutagenesisi118R → A: Strongly reduced DNA binding. 1 Publication1
Mutagenesisi149T → A: Strongly reduced DNA binding. 1 Publication1
Mutagenesisi150N → A or D: Strongly reduced DNA binding. 1 Publication1
Mutagenesisi151T → A: Reduced DNA binding. 1 Publication1
Mutagenesisi152N → D: Strongly reduced DNA binding. 1 Publication1
Mutagenesisi155R → A: Strongly reduced DNA binding. 1 Publication1
Mutagenesisi156K → A: Strongly reduced DNA binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000976841 – 181Negative modulator of initiation of replicationAdd BLAST181

Proteomic databases

EPDiP0AFY8.
PaxDbiP0AFY8.
PRIDEiP0AFY8.

Interactioni

Subunit structurei

Homodimer. Polymerizes to form helical filaments. Dimerization is sufficient for DNA-binding, but oligomerization is essential for function.UniRule annotation4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself13EBI-552553,EBI-552553
uvrBP0A8F82EBI-552553,EBI-552176

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4261907. 82 interactors.
DIPiDIP-48017N.
IntActiP0AFY8. 3 interactors.
MINTiMINT-1238758.
STRINGi511145.b0687.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi8 – 15Combined sources8
Helixi25 – 33Combined sources9
Helixi36 – 38Combined sources3
Helixi71 – 77Combined sources7
Helixi79 – 83Combined sources5
Helixi87 – 101Combined sources15
Helixi103 – 111Combined sources9
Beta strandi116 – 119Combined sources4
Beta strandi121 – 125Combined sources5
Helixi126 – 130Combined sources5
Beta strandi131 – 134Combined sources4
Beta strandi137 – 139Combined sources3
Beta strandi142 – 144Combined sources3
Beta strandi146 – 148Combined sources3
Helixi153 – 166Combined sources14
Helixi171 – 178Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IU3X-ray3.00C/F71-181[»]
1J3EX-ray2.50A71-181[»]
1LRRX-ray2.65A/D51-181[»]
1XRXX-ray2.15A/B/C/D1-50[»]
2CH3model-A1-181[»]
3FMTX-ray2.98A/B/E/F1-181[»]
ProteinModelPortaliP0AFY8.
SMRiP0AFY8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFY8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 88Interaction with DNA2
Regioni116 – 120Interaction with DNA5
Regioni150 – 156Interaction with DNA7

Domaini

The N-terminal domain is required for multimerization. The C-terminal domain is involved in DNA-binding.3 Publications

Sequence similaritiesi

Belongs to the SeqA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107CDU. Bacteria.
COG3057. LUCA.
HOGENOMiHOG000275226.
KOiK03645.
OMAiRTRTYFA.

Family and domain databases

Gene3Di1.10.1220.10. 1 hit.
1.20.1380.10. 1 hit.
HAMAPiMF_00908. SeqA. 1 hit.
InterProiIPR013321. Arc_rbn_hlx_hlx.
IPR010985. Ribbon_hlx_hlx.
IPR005621. SeqA.
IPR026577. SeqA_DNA-bd_C.
IPR033761. SeqA_N.
[Graphical view]
PfamiPF03925. SeqA. 1 hit.
PF17206. SeqA_N. 1 hit.
[Graphical view]
PIRSFiPIRSF019401. SeqA. 1 hit.
SUPFAMiSSF47598. SSF47598. 1 hit.
SSF82808. SSF82808. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTIEVDDEL YSYIASHTKH IGESASDILR RMLKFSAASQ PAAPVTKEVR
60 70 80 90 100
VASPAIVEAK PVKTIKDKVR AMRELLLSDE YAEQKRAVNR FMLLLSTLYS
110 120 130 140 150
LDAQAFAEAT ESLHGRTRVY FAADEQTLLK NGNQTKPKHV PGTPYWVITN
160 170 180
TNTGRKCSMI EHIMQSMQFP AELIEKVCGT I
Length:181
Mass (Da):20,315
Last modified:December 20, 2005 - v1
Checksum:i37D9078CA3D7229B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07651 Unassigned RNA. Translation: AAA19855.1.
U00096 Genomic DNA. Translation: AAC73781.1.
AP009048 Genomic DNA. Translation: BAA35336.1.
PIRiA54296.
RefSeqiNP_415213.1. NC_000913.3.
WP_000848387.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73781; AAC73781; b0687.
BAA35336; BAA35336; BAA35336.
GeneIDi945272.
KEGGiecj:JW0674.
eco:b0687.
PATRICi32116567. VBIEscCol129921_0716.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07651 Unassigned RNA. Translation: AAA19855.1.
U00096 Genomic DNA. Translation: AAC73781.1.
AP009048 Genomic DNA. Translation: BAA35336.1.
PIRiA54296.
RefSeqiNP_415213.1. NC_000913.3.
WP_000848387.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IU3X-ray3.00C/F71-181[»]
1J3EX-ray2.50A71-181[»]
1LRRX-ray2.65A/D51-181[»]
1XRXX-ray2.15A/B/C/D1-50[»]
2CH3model-A1-181[»]
3FMTX-ray2.98A/B/E/F1-181[»]
ProteinModelPortaliP0AFY8.
SMRiP0AFY8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261907. 82 interactors.
DIPiDIP-48017N.
IntActiP0AFY8. 3 interactors.
MINTiMINT-1238758.
STRINGi511145.b0687.

Proteomic databases

EPDiP0AFY8.
PaxDbiP0AFY8.
PRIDEiP0AFY8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73781; AAC73781; b0687.
BAA35336; BAA35336; BAA35336.
GeneIDi945272.
KEGGiecj:JW0674.
eco:b0687.
PATRICi32116567. VBIEscCol129921_0716.

Organism-specific databases

EchoBASEiEB2114.
EcoGeneiEG12197. seqA.

Phylogenomic databases

eggNOGiENOG4107CDU. Bacteria.
COG3057. LUCA.
HOGENOMiHOG000275226.
KOiK03645.
OMAiRTRTYFA.

Enzyme and pathway databases

BioCyciEcoCyc:EG12197-MONOMER.
ECOL316407:JW0674-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFY8.
PROiP0AFY8.

Family and domain databases

Gene3Di1.10.1220.10. 1 hit.
1.20.1380.10. 1 hit.
HAMAPiMF_00908. SeqA. 1 hit.
InterProiIPR013321. Arc_rbn_hlx_hlx.
IPR010985. Ribbon_hlx_hlx.
IPR005621. SeqA.
IPR026577. SeqA_DNA-bd_C.
IPR033761. SeqA_N.
[Graphical view]
PfamiPF03925. SeqA. 1 hit.
PF17206. SeqA_N. 1 hit.
[Graphical view]
PIRSFiPIRSF019401. SeqA. 1 hit.
SUPFAMiSSF47598. SSF47598. 1 hit.
SSF82808. SSF82808. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSEQA_ECOLI
AccessioniPrimary (citable) accession number: P0AFY8
Secondary accession number(s): P36658
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.