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Protein

Peptide antibiotic transporter SbmA

Gene

sbmA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Uptake of antimicrobial peptides. Required for the transport of microcin B17 (MccB17), microcin 25 (Mcc25) and proline-rich antimicrobial peptides into the cell.3 Publications

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: InterPro
  • ATP binding Source: InterPro
  • microcin uptake transporter activity Source: EcoCyc
  • peptide transporter activity Source: EcoCyc
  • secondary active transmembrane transporter activity Source: EcoCyc
  • toxin transporter activity Source: EcoCyc

GO - Biological processi

  • microcin B17 transport Source: EcoCyc
  • microcin transport Source: EcoCyc
  • peptide transport Source: EcoCyc
  • protein transport Source: UniProtKB-KW
  • response to antibiotic Source: EcoCyc
  • toxin transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Peptide transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:SBMA-MONOMER.
ECOL316407:JW0368-MONOMER.

Protein family/group databases

TCDBi9.A.18.1.1. the peptide uptake permease (pup) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide antibiotic transporter SbmA
Gene namesi
Name:sbmA
Ordered Locus Names:b0377, JW0368
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10928. sbmA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11PeriplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Topological domaini33 – 56CytoplasmicSequence analysisAdd BLAST24
Transmembranei57 – 77HelicalSequence analysisAdd BLAST21
Topological domaini78 – 87PeriplasmicSequence analysis10
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Topological domaini109 – 137CytoplasmicSequence analysisAdd BLAST29
Transmembranei138 – 158HelicalSequence analysisAdd BLAST21
Topological domaini159 – 205PeriplasmicSequence analysisAdd BLAST47
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21
Topological domaini227 – 242CytoplasmicSequence analysisAdd BLAST16
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 331PeriplasmicSequence analysisAdd BLAST68
Transmembranei332 – 352HelicalSequence analysisAdd BLAST21
Topological domaini353 – 406CytoplasmicSequence analysisAdd BLAST54

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants are resistant to proline-rich antimicrobial peptides of eukaryotic origin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000976031 – 406Peptide antibiotic transporter SbmAAdd BLAST406

Proteomic databases

PaxDbiP0AFY6.
PRIDEiP0AFY6.

Interactioni

Protein-protein interaction databases

BioGridi4259483. 94 interactors.
STRINGi511145.b0377.

Structurei

3D structure databases

ProteinModelPortaliP0AFY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108DQG. Bacteria.
COG1133. LUCA.
HOGENOMiHOG000100637.
InParanoidiP0AFY6.
KOiK17938.
OMAiSFFPQPK.
PhylomeDBiP0AFY6.

Family and domain databases

InterProiIPR011527. ABC1_TM_dom.
IPR009248. SbmA_BacA.
[Graphical view]
PfamiPF05992. SbmA_BacA. 1 hit.
[Graphical view]
SUPFAMiSSF90123. SSF90123. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKSFFPKPG TFFLSAFVWA LIAVIFWQAG GGDWVARITG ASGQIPISAA
60 70 80 90 100
RFWSLDFLIF YAYYIVCVGL FALFWFIYSP HRWQYWSILG TALIIFVTWF
110 120 130 140 150
LVEVGVAVNA WYAPFYDLIQ TALSSPHKVT IEQFYREVGV FLGIALIAVV
160 170 180 190 200
ISVLNNFFVS HYVFRWRTAM NEYYMANWQQ LRHIEGAAQR VQEDTMRFAS
210 220 230 240 250
TLENMGVSFI NAIMTLIAFL PVLVTLSAHV PELPIIGHIP YGLVIAAIVW
260 270 280 290 300
SLMGTGLLAV VGIKLPGLEF KNQRVEAAYR KELVYGEDDA TRATPPTVRE
310 320 330 340 350
LFSAVRKNYF RLYFHYMYFN IARILYLQVD NVFGLFLLFP SIVAGTITLG
360 370 380 390 400
LMTQITNVFG QVRGAFQYLI NSWTTLVELM SIYKRLRSFE HELDGDKIQE

VTHTLS
Length:406
Mass (Da):46,459
Last modified:December 20, 2005 - v1
Checksum:i684057C831F624CD
GO

Sequence cautioni

The sequence AAB18100 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti388S → T in CAA38092 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54153 Genomic DNA. Translation: CAA38092.1.
U73857 Genomic DNA. Translation: AAB18100.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73480.1.
AP009048 Genomic DNA. Translation: BAE76158.1.
PIRiA64766.
RefSeqiNP_414911.1. NC_000913.3.
WP_001301663.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73480; AAC73480; b0377.
BAE76158; BAE76158; BAE76158.
GeneIDi946884.
KEGGiecj:JW0368.
eco:b0377.
PATRICi32115895. VBIEscCol129921_0389.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54153 Genomic DNA. Translation: CAA38092.1.
U73857 Genomic DNA. Translation: AAB18100.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73480.1.
AP009048 Genomic DNA. Translation: BAE76158.1.
PIRiA64766.
RefSeqiNP_414911.1. NC_000913.3.
WP_001301663.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259483. 94 interactors.
STRINGi511145.b0377.

Protein family/group databases

TCDBi9.A.18.1.1. the peptide uptake permease (pup) family.

Proteomic databases

PaxDbiP0AFY6.
PRIDEiP0AFY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73480; AAC73480; b0377.
BAE76158; BAE76158; BAE76158.
GeneIDi946884.
KEGGiecj:JW0368.
eco:b0377.
PATRICi32115895. VBIEscCol129921_0389.

Organism-specific databases

EchoBASEiEB0921.
EcoGeneiEG10928. sbmA.

Phylogenomic databases

eggNOGiENOG4108DQG. Bacteria.
COG1133. LUCA.
HOGENOMiHOG000100637.
InParanoidiP0AFY6.
KOiK17938.
OMAiSFFPQPK.
PhylomeDBiP0AFY6.

Enzyme and pathway databases

BioCyciEcoCyc:SBMA-MONOMER.
ECOL316407:JW0368-MONOMER.

Miscellaneous databases

PROiP0AFY6.

Family and domain databases

InterProiIPR011527. ABC1_TM_dom.
IPR009248. SbmA_BacA.
[Graphical view]
PfamiPF05992. SbmA_BacA. 1 hit.
[Graphical view]
SUPFAMiSSF90123. SSF90123. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSBMA_ECOLI
AccessioniPrimary (citable) accession number: P0AFY6
Secondary accession number(s): P24212
, P71313, P75702, Q2MC48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.