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Protein

Regulator of sigma D

Gene

rsd

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds RpoD and negatively regulates RpoD-mediated transcription activation by preventing the interaction between the primary sigma factor RpoD with the catalytic core of the RNA polymerase and with promoter DNA. May be involved in replacement of the RNA polymerase sigma subunit from RpoD to RpoS during the transition from exponential growth to the stationary phase.UniRule annotation2 Publications

GO - Molecular functioni

  • sigma factor antagonist activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG11738-MONOMER.
ECOL316407:JW3959-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of sigma DUniRule annotation
Gene namesi
Name:rsdUniRule annotation
Ordered Locus Names:b3995, JW3959
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11738. rsd.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000974761 – 158Regulator of sigma DAdd BLAST158

Proteomic databases

EPDiP0AFX4.
PaxDbiP0AFX4.
PRIDEiP0AFX4.

Interactioni

Subunit structurei

Interacts with RpoD.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
rpoDP0057911EBI-1134364,EBI-545104

Protein-protein interaction databases

DIPiDIP-10800N.
IntActiP0AFX4. 8 interactors.
STRINGi511145.b3995.

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 12Combined sources12
Helixi17 – 37Combined sources21
Helixi46 – 49Combined sources4
Helixi51 – 70Combined sources20
Helixi72 – 79Combined sources8
Helixi83 – 108Combined sources26
Helixi110 – 117Combined sources8
Helixi120 – 150Combined sources31

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P7VX-ray2.60A1-158[»]
4XWJX-ray2.10A1-151[»]
ProteinModelPortaliP0AFX4.
SMRiP0AFX4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFX4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 71Interaction with RpoDAdd BLAST13
Regioni101 – 108Interaction with RpoD8

Sequence similaritiesi

Belongs to the Rsd/AlgQ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107MNS. Bacteria.
COG3160. LUCA.
HOGENOMiHOG000283202.
InParanoidiP0AFX4.
KOiK07740.
OMAiLESCQNA.

Family and domain databases

HAMAPiMF_01181. Rsd. 1 hit.
InterProiIPR023785. Sigma70_reg_Rsd.
IPR007448. Sigma70_reg_Rsd_AlgQ.
[Graphical view]
PfamiPF04353. Rsd_AlgQ. 1 hit.
[Graphical view]
PIRSFiPIRSF016548. Rsd_AlgQ. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFX4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNQLDNLTE RVRGSNKLVD RWLHVRKHLL VAYYNLVGIK PGKESYMRLN
60 70 80 90 100
EKALDDFCQS LVDYLSAGHF SIYERILHKL EGNGQLARAA KIWPQLEANT
110 120 130 140 150
QQIMDYYDSS LETAIDHDNY LEFQQVLSDI GEALEARFVL EDKLILLVLD

AARVKHPA
Length:158
Mass (Da):18,243
Last modified:December 20, 2005 - v1
Checksum:iBDB699DAC440A808
GO

Sequence cautioni

The sequence D12624 differs from that shown. Reason: Frameshift at position 137.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12624 Genomic DNA. No translation available.
U00006 Genomic DNA. Translation: AAC43093.1.
U00096 Genomic DNA. Translation: AAC76969.1.
AP009048 Genomic DNA. Translation: BAE77324.1.
PIRiF65206.
RefSeqiNP_418423.1. NC_000913.3.
WP_000934302.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76969; AAC76969; b3995.
BAE77324; BAE77324; BAE77324.
GeneIDi948496.
KEGGiecj:JW3959.
eco:b3995.
PATRICi32123513. VBIEscCol129921_4109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12624 Genomic DNA. No translation available.
U00006 Genomic DNA. Translation: AAC43093.1.
U00096 Genomic DNA. Translation: AAC76969.1.
AP009048 Genomic DNA. Translation: BAE77324.1.
PIRiF65206.
RefSeqiNP_418423.1. NC_000913.3.
WP_000934302.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P7VX-ray2.60A1-158[»]
4XWJX-ray2.10A1-151[»]
ProteinModelPortaliP0AFX4.
SMRiP0AFX4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10800N.
IntActiP0AFX4. 8 interactors.
STRINGi511145.b3995.

Proteomic databases

EPDiP0AFX4.
PaxDbiP0AFX4.
PRIDEiP0AFX4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76969; AAC76969; b3995.
BAE77324; BAE77324; BAE77324.
GeneIDi948496.
KEGGiecj:JW3959.
eco:b3995.
PATRICi32123513. VBIEscCol129921_4109.

Organism-specific databases

EchoBASEiEB1689.
EcoGeneiEG11738. rsd.

Phylogenomic databases

eggNOGiENOG4107MNS. Bacteria.
COG3160. LUCA.
HOGENOMiHOG000283202.
InParanoidiP0AFX4.
KOiK07740.
OMAiLESCQNA.

Enzyme and pathway databases

BioCyciEcoCyc:EG11738-MONOMER.
ECOL316407:JW3959-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFX4.
PROiP0AFX4.

Family and domain databases

HAMAPiMF_01181. Rsd. 1 hit.
InterProiIPR023785. Sigma70_reg_Rsd.
IPR007448. Sigma70_reg_Rsd_AlgQ.
[Graphical view]
PfamiPF04353. Rsd_AlgQ. 1 hit.
[Graphical view]
PIRSFiPIRSF016548. Rsd_AlgQ. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSD_ECOLI
AccessioniPrimary (citable) accession number: P0AFX4
Secondary accession number(s): P31690, Q2M8T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.