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Protein

Regulator of sigma D

Gene

rsd

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds RpoD and negatively regulates RpoD-mediated transcription activation by preventing the interaction between the primary sigma factor RpoD with the catalytic core of the RNA polymerase and with promoter DNA. May be involved in replacement of the RNA polymerase sigma subunit from RpoD to RpoS during the transition from exponential growth to the stationary phase.UniRule annotation2 Publications

GO - Molecular functioni

  • sigma factor antagonist activity Source: EcoCyc

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG11738-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of sigma DUniRule annotation
Gene namesi
Name:rsdUniRule annotation
Ordered Locus Names:b3995, JW3959
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11738 rsd

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000974761 – 158Regulator of sigma DAdd BLAST158

Proteomic databases

EPDiP0AFX4
PaxDbiP0AFX4
PRIDEiP0AFX4

Interactioni

Subunit structurei

Interacts with RpoD.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
rpoDP0057911EBI-1134364,EBI-545104

Protein-protein interaction databases

BioGridi42593341 interactor.
DIPiDIP-10800N
IntActiP0AFX4 8 interactors.
STRINGi316385.ECDH10B_4184

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 12Combined sources12
Helixi17 – 37Combined sources21
Helixi46 – 49Combined sources4
Helixi51 – 70Combined sources20
Helixi72 – 79Combined sources8
Helixi83 – 108Combined sources26
Helixi110 – 117Combined sources8
Helixi120 – 150Combined sources31

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P7VX-ray2.60A1-158[»]
4XWJX-ray2.10A1-151[»]
ProteinModelPortaliP0AFX4
SMRiP0AFX4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFX4

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 71Interaction with RpoDAdd BLAST13
Regioni101 – 108Interaction with RpoD8

Sequence similaritiesi

Belongs to the Rsd/AlgQ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107MNS Bacteria
COG3160 LUCA
HOGENOMiHOG000283202
InParanoidiP0AFX4
KOiK07740
OMAiMLESCQN

Family and domain databases

Gene3Di1.20.120.13701 hit
HAMAPiMF_01181 Rsd, 1 hit
InterProiView protein in InterPro
IPR038309 Rsd/AlgQ_sf
IPR023785 Sigma70_reg_Rsd
IPR007448 Sigma70_reg_Rsd_AlgQ
PfamiView protein in Pfam
PF04353 Rsd_AlgQ, 1 hit
PIRSFiPIRSF016548 Rsd_AlgQ, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFX4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNQLDNLTE RVRGSNKLVD RWLHVRKHLL VAYYNLVGIK PGKESYMRLN
60 70 80 90 100
EKALDDFCQS LVDYLSAGHF SIYERILHKL EGNGQLARAA KIWPQLEANT
110 120 130 140 150
QQIMDYYDSS LETAIDHDNY LEFQQVLSDI GEALEARFVL EDKLILLVLD

AARVKHPA
Length:158
Mass (Da):18,243
Last modified:December 20, 2005 - v1
Checksum:iBDB699DAC440A808
GO

Sequence cautioni

The sequence D12624 differs from that shown. Reason: Frameshift at position 137.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12624 Genomic DNA No translation available.
U00006 Genomic DNA Translation: AAC43093.1
U00096 Genomic DNA Translation: AAC76969.1
AP009048 Genomic DNA Translation: BAE77324.1
PIRiF65206
RefSeqiNP_418423.1, NC_000913.3
WP_000934302.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76969; AAC76969; b3995
BAE77324; BAE77324; BAE77324
GeneIDi948496
KEGGiecj:JW3959
eco:b3995
PATRICifig|1411691.4.peg.2716

Similar proteinsi

Entry informationi

Entry nameiRSD_ECOLI
AccessioniPrimary (citable) accession number: P0AFX4
Secondary accession number(s): P31690, Q2M8T2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome