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P0AFX0 (RP5M_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable sigma(54) modulation protein
Alternative name(s):
ORF3
ORF95
Gene names
Name:yhbH
Ordered Locus Names:b3203, JW3170
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length95 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably modulates the activity of sigma(54) (rpoN).

Sequence similarities

Belongs to the sigma(54) modulation protein family.

Ontologies

Keywords
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processprimary metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionbinding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9595Probable sigma(54) modulation protein
PRO_0000097417

Secondary structure

............... 95
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AFX0 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 5B8F737E6E3A91AE

FASTA9510,750
        10         20         30         40         50         60 
MQLNITGNNV EITEALREFV TAKFAKLEQY FDRINQVYVV LKVEKVTHTS DATLHVNGGE 

        70         80         90 
IHASAEGQDM YAAIDGLIDK LARQLTKHKD KLKQH 

« Hide

References

« Hide 'large scale' references
[1]"Physical map location of the rpoN gene of Escherichia coli."
Imaishi H., Gomada M., Inouye S., Nakazawa A.
J. Bacteriol. 175:1550-1551(1993) [PubMed: 8444818] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Molecular analysis of the operon which encodes the RNA polymerase sigma factor sigma 54 of Escherichia coli."
Jones D.H.A., Franklin C.F.H., Thomas C.M.
Microbiology 140:1035-1043(1994) [PubMed: 8025669] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant."
Powell B.S., Court D.L., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier M.H. Jr., Reizer J.
J. Biol. Chem. 270:4822-4839(1995) [PubMed: 7876255] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
Electrophoresis 20:2181-2195(1999) [PubMed: 10493123] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
Strain: B / BL21.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D12938 Genomic DNA. Translation: BAA02316.1.
Z27094 Genomic DNA. Translation: CAA81618.1.
U12684 Genomic DNA. Translation: AAB60164.1.
U18997 Genomic DNA. Translation: AAA58005.1.
U00096 Genomic DNA. Translation: AAC76235.1.
AP009048 Genomic DNA. Translation: BAE77247.1.
PIRI76719.
RefSeqNP_417670.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2RQLNMR-A1-95[»]
ProteinModelPortalP0AFX0.
SMRP0AFX0. Positions 1-95.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-48273N.
IntActP0AFX0. 4 interactions.
MINTMINT-1249576.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000003007; EBESCP00000003007; EBESCG00000002466.
EBESCT00000003008; EBESCP00000003008; EBESCG00000002466.
EBESCT00000016487; EBESCP00000015778; EBESCG00000015547.
GeneID947718.
GenomeReviewsGene locus JW3170 in contig AP009048_GR.
Gene locus b3203 in contig U00096_GR.
KEGGecj:JW3170.
eco:b3203.
PATRIC32121826. VBIEscCol129921_3297.

Organism-specific databases

EchoBASEEB1632.
EcoGeneEG11681. yhbH.

Phylogenomic databases

eggNOGCOG1544.
GeneTreeEBGT00050000009890.
HOGENOMHBG675832.
OMADQINNVH.
PhylomeDBP0AFX0.
ProtClustDBPRK10470.

Enzyme and pathway databases

BioCycEcoCyc:EG11681-MONOMER.

Gene expression databases

GenevestigatorP0AFX0.

Family and domain databases

InterProIPR003489. Ribosomal_S30Ae/sigma54_mod.
[Graphical view]
Gene3DG3DSA:3.30.160.100. Ribosomal_S30Ae/sigma54_mod. 1 hit.
KOK05808.
PfamPF02482. Ribosomal_S30AE. 1 hit.
[Graphical view]
SUPFAMSSF69754. Ribosomal_S30Ae/sigma54_mod. 1 hit.
TIGRFAMsTIGR00741. YfiA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRP5M_ECOLI
AccessionPrimary (citable) accession number: P0AFX0
Secondary accession number(s): P31221, Q2M909
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families