Reviewed, UniProtKB/Swiss-Prot P0AFX0 (RP5M_ECOLI)

Names and origin

Protein names

Recommended name:
    Probable sigma(54) modulation protein
Alternative name(s):
    ORF3
    ORF95

Gene names

Name:	yhbH
Ordered Locus Names:	b3203, JW3170

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably modulates the activity of sigma(54) (rpoN).
Sequence similarities	Belongs to the sigma(54) modulation protein family.

Ontologies

Keywords
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	primary metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dnaN	P0A988	1	EBI-561113,EBI-542385

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 95	95	Probable sigma(54) modulation protein		PRO_0000097417

Sequences

	Sequence		Length	Mass (Da)
	P0AFX0-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 5B8F737E6E3A91AE	FASTA	95	10,750
	10 20 30 40 50 60 MQLNITGNNV EITEALREFV TAKFAKLEQY FDRINQVYVV LKVEKVTHTS DATLHVNGGE 70 80 90 IHASAEGQDM YAAIDGLIDK LARQLTKHKD KLKQH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Physical map location of the rpoN gene of Escherichia coli." Imaishi H., Gomada M., Inouye S., Nakazawa A. J. Bacteriol. 175:1550-1551(1993) [PubMed: 8444818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Molecular analysis of the operon which encodes the RNA polymerase sigma factor sigma 54 of Escherichia coli." Jones D.H.A., Franklin C.F.H., Thomas C.M. Microbiology 140:1035-1043(1994) [PubMed: 8025669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant." Powell B.S., Court D.L., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier M.H. Jr., Reizer J. J. Biol. Chem. 270:4822-4839(1995) [PubMed: 7876255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B. Electrophoresis 20:2181-2195(1999) [PubMed: 10493123] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D12938 Genomic DNA. Translation: BAA02316.1. Z27094 Genomic DNA. Translation: CAA81618.1. U12684 Genomic DNA. Translation: AAB60164.1. U18997 Genomic DNA. Translation: AAA58005.1. U00096 Genomic DNA. Translation: AAC76235.1. AP009048 Genomic DNA. Translation: BAE77247.1.
PIR	I76719.
RefSeq	AP_003746.1. NP_417670.1.
3D structure databases
HSSP	HSSP built from PDB template 1N3G based on UniProtKB P11285.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AFX0. 4 interactions.
Genome annotation databases
GeneID	947718.
GenomeReviews	Gene locus JW3170 in contig AP009048_GR. Gene locus b3203 in contig U00096_GR.
KEGG	ecj:JW3170. eco:b3203.
Organism-specific databases
EchoBASE	EB1632.
EcoGene	EG11681. yhbH.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AFX0.
OMA	P0AFX0. LTISGHH.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11681-MON.
Family and domain databases
InterPro	IPR003489. Ribosomal_S30Ae/sigma54_mod. [Graphical view]
Gene3D	G3DSA:3.30.160.100. Ribosomal_S30Ae/sigma54_mod. 1 hit.
Pfam	PF02482. Ribosomal_S30AE. 1 hit. [Graphical view]
TIGRFAMs	TIGR00741. yfiA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RP5M_ECOLI

Accession

Primary (citable) accession number: P0AFX0
Secondary accession number(s): P31221, Q2M909

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)