Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein rof

Gene

rof

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Suppresses temperature-sensitive mutations in essential genes by modulating rho-dependent transcription termination.

GO - Biological processi

  • negative regulation of DNA-templated transcription, termination Source: EcoCyc
  • regulation of DNA-templated transcription, termination Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G6097-MONOMER.
ECOL316407:JW0184-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein rof
Gene namesi
Name:rof
Synonyms:yaeO
Ordered Locus Names:b0189, JW0184
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13221. rof.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000973971 – 84Protein rofAdd BLAST84

Proteomic databases

EPDiP0AFW8.
PaxDbiP0AFW8.
PRIDEiP0AFW8.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
rhoP0AG302EBI-1114609,EBI-545468

Protein-protein interaction databases

BioGridi4260946. 399 interactors.
IntActiP0AFW8. 11 interactors.
MINTiMINT-8200743.
STRINGi511145.b0189.

Structurei

Secondary structure

184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 20Combined sources8
Turni21 – 24Combined sources4
Beta strandi26 – 29Combined sources4
Turni31 – 33Combined sources3
Beta strandi36 – 40Combined sources5
Beta strandi42 – 44Combined sources3
Beta strandi47 – 49Combined sources3
Beta strandi51 – 56Combined sources6
Beta strandi59 – 64Combined sources6
Beta strandi69 – 73Combined sources5
Turni74 – 76Combined sources3
Beta strandi77 – 79Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SG5NMR-A1-84[»]
ProteinModelPortaliP0AFW8.
SMRiP0AFW8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFW8.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105M1T. Bacteria.
COG4568. LUCA.
HOGENOMiHOG000001314.
KOiK19000.
OMAiGGKEEYL.

Family and domain databases

InterProiIPR009778. ROF.
IPR023534. Rof/RNase_P-like.
[Graphical view]
PfamiPF07073. ROF. 1 hit.
[Graphical view]
SUPFAMiSSF101744. SSF101744. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDTYQPINC DDYDNLELAC QHHLMLTLEL KDGEKLQAKA SDLVSRKNVE
60 70 80
YLVVEAAGET RELRLDKITS FSHPEIGTVV VSES
Length:84
Mass (Da):9,480
Last modified:December 20, 2005 - v1
Checksum:i1BD1E254B0F0A82E
GO

Sequence cautioni

The sequence CAA90752 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50870 Genomic DNA. Translation: CAA90752.1. Different initiation.
D49445 Genomic DNA. Translation: BAA08429.1.
U00096 Genomic DNA. Translation: AAC73300.2.
AP009048 Genomic DNA. Translation: BAA77864.2.
RefSeqiNP_414731.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73300; AAC73300; b0189.
BAA77864; BAA77864; BAA77864.
GeneIDi944891.
KEGGiecj:JW0184.
eco:b0189.
PATRICi32115489. VBIEscCol129921_0196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50870 Genomic DNA. Translation: CAA90752.1. Different initiation.
D49445 Genomic DNA. Translation: BAA08429.1.
U00096 Genomic DNA. Translation: AAC73300.2.
AP009048 Genomic DNA. Translation: BAA77864.2.
RefSeqiNP_414731.2. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SG5NMR-A1-84[»]
ProteinModelPortaliP0AFW8.
SMRiP0AFW8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260946. 399 interactors.
IntActiP0AFW8. 11 interactors.
MINTiMINT-8200743.
STRINGi511145.b0189.

Proteomic databases

EPDiP0AFW8.
PaxDbiP0AFW8.
PRIDEiP0AFW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73300; AAC73300; b0189.
BAA77864; BAA77864; BAA77864.
GeneIDi944891.
KEGGiecj:JW0184.
eco:b0189.
PATRICi32115489. VBIEscCol129921_0196.

Organism-specific databases

EchoBASEiEB3012.
EcoGeneiEG13221. rof.

Phylogenomic databases

eggNOGiENOG4105M1T. Bacteria.
COG4568. LUCA.
HOGENOMiHOG000001314.
KOiK19000.
OMAiGGKEEYL.

Enzyme and pathway databases

BioCyciEcoCyc:G6097-MONOMER.
ECOL316407:JW0184-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFW8.
PROiP0AFW8.

Family and domain databases

InterProiIPR009778. ROF.
IPR023534. Rof/RNase_P-like.
[Graphical view]
PfamiPF07073. ROF. 1 hit.
[Graphical view]
SUPFAMiSSF101744. SSF101744. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiROF_ECOLI
AccessioniPrimary (citable) accession number: P0AFW8
Secondary accession number(s): P52098, P75670, Q9WVU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.