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Protein

Protein rof

Gene

rof

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Suppresses temperature-sensitive mutations in essential genes by modulating rho-dependent transcription termination.

GO - Biological processi

  • negative regulation of DNA-templated transcription, termination Source: EcoCyc
  • regulation of DNA-templated transcription, termination Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G6097-MONOMER.
ECOL316407:JW0184-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein rof
Gene namesi
Name:rof
Synonyms:yaeO
Ordered Locus Names:b0189, JW0184
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13221. rof.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8484Protein rofPRO_0000097397Add
BLAST

Proteomic databases

EPDiP0AFW8.
PaxDbiP0AFW8.
PRIDEiP0AFW8.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
rhoP0AG302EBI-1114609,EBI-545468

Protein-protein interaction databases

BioGridi4260946. 399 interactions.
IntActiP0AFW8. 11 interactions.
MINTiMINT-8200743.
STRINGi511145.b0189.

Structurei

Secondary structure

1
84
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 208Combined sources
Turni21 – 244Combined sources
Beta strandi26 – 294Combined sources
Turni31 – 333Combined sources
Beta strandi36 – 405Combined sources
Beta strandi42 – 443Combined sources
Beta strandi47 – 493Combined sources
Beta strandi51 – 566Combined sources
Beta strandi59 – 646Combined sources
Beta strandi69 – 735Combined sources
Turni74 – 763Combined sources
Beta strandi77 – 793Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SG5NMR-A1-84[»]
ProteinModelPortaliP0AFW8.
SMRiP0AFW8. Positions 1-84.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFW8.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105M1T. Bacteria.
COG4568. LUCA.
HOGENOMiHOG000001314.
KOiK19000.
OMAiGGKEEYL.
OrthoDBiEOG6H7FN8.

Family and domain databases

InterProiIPR009778. ROF.
IPR023534. Rof/RNase_P-like.
[Graphical view]
PfamiPF07073. ROF. 1 hit.
[Graphical view]
SUPFAMiSSF101744. SSF101744. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDTYQPINC DDYDNLELAC QHHLMLTLEL KDGEKLQAKA SDLVSRKNVE
60 70 80
YLVVEAAGET RELRLDKITS FSHPEIGTVV VSES
Length:84
Mass (Da):9,480
Last modified:December 20, 2005 - v1
Checksum:i1BD1E254B0F0A82E
GO

Sequence cautioni

The sequence CAA90752.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50870 Genomic DNA. Translation: CAA90752.1. Different initiation.
D49445 Genomic DNA. Translation: BAA08429.1.
U00096 Genomic DNA. Translation: AAC73300.2.
AP009048 Genomic DNA. Translation: BAA77864.2.
RefSeqiNP_414731.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73300; AAC73300; b0189.
BAA77864; BAA77864; BAA77864.
GeneIDi944891.
KEGGiecj:JW0184.
eco:b0189.
PATRICi32115489. VBIEscCol129921_0196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50870 Genomic DNA. Translation: CAA90752.1. Different initiation.
D49445 Genomic DNA. Translation: BAA08429.1.
U00096 Genomic DNA. Translation: AAC73300.2.
AP009048 Genomic DNA. Translation: BAA77864.2.
RefSeqiNP_414731.2. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SG5NMR-A1-84[»]
ProteinModelPortaliP0AFW8.
SMRiP0AFW8. Positions 1-84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260946. 399 interactions.
IntActiP0AFW8. 11 interactions.
MINTiMINT-8200743.
STRINGi511145.b0189.

Proteomic databases

EPDiP0AFW8.
PaxDbiP0AFW8.
PRIDEiP0AFW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73300; AAC73300; b0189.
BAA77864; BAA77864; BAA77864.
GeneIDi944891.
KEGGiecj:JW0184.
eco:b0189.
PATRICi32115489. VBIEscCol129921_0196.

Organism-specific databases

EchoBASEiEB3012.
EcoGeneiEG13221. rof.

Phylogenomic databases

eggNOGiENOG4105M1T. Bacteria.
COG4568. LUCA.
HOGENOMiHOG000001314.
KOiK19000.
OMAiGGKEEYL.
OrthoDBiEOG6H7FN8.

Enzyme and pathway databases

BioCyciEcoCyc:G6097-MONOMER.
ECOL316407:JW0184-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFW8.
PROiP0AFW8.

Family and domain databases

InterProiIPR009778. ROF.
IPR023534. Rof/RNase_P-like.
[Graphical view]
PfamiPF07073. ROF. 1 hit.
[Graphical view]
SUPFAMiSSF101744. SSF101744. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An Escherichia coli gene (yaeO) suppresses temperature-sensitive mutations in essential genes by modulating Rho-dependent transcription termination."
    Pichoff S., Alibaud L., Guedant A., Castanie M.-P., Bouche J.-P.
    Mol. Microbiol. 29:859-869(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: K12.
  2. Yamamoto Y.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
    Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 72-73.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiROF_ECOLI
AccessioniPrimary (citable) accession number: P0AFW8
Secondary accession number(s): P52098, P75670, Q9WVU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 16, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.