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P0AFW2 (RMF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosome modulation factor

Short name=Protein E
Gene names
Name:rmf
Ordered Locus Names:b0953, JW0936
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length55 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Associates with 70S ribosomes and converts them to a dimeric form (100S ribosomes) which appears during the transition from the exponential growth phase to the stationary phase of E.coli cells.

Sequence caution

The sequence J03186 differs from that shown. Reason: Frameshift at positions 4, 11 and 30.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5555Ribosome modulation factor
PRO_0000097363

Experimental info

Sequence conflict31R → I AA sequence Ref.6
Sequence conflict251S → V AA sequence Ref.6
Sequence conflict331T → S Ref.1
Sequence conflict331T → S Ref.2

Sequences

Sequence LengthMass (Da)Tools
P0AFW2 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: D264AF285BBF24AB

FASTA556,507
        10         20         30         40         50 
MKRQKRDRLE RAHQRGYQAG IAGRSKEMCP YQTLNQRSQW LGGWREAMAD RVVMA 

« Hide

References

« Hide 'large scale' references
[1]"Regulation of the Escherichia coli rmf gene encoding the ribosome modulation factor: growth phase- and growth rate-dependent control."
Yamagishi M., Matsushima H., Wada A., Sakagami M., Fujita N., Ishihama A.
EMBO J. 12:625-630(1993) [PubMed: 8440252] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Derived amino acid sequence and identification of active site residues of Escherichia coli beta-hydroxydecanoyl thioester dehydrase."
Cronan J.E. Jr., Li W.-B., Coleman R., Narasimhan M., de Mendoza D., Schwab J.M.
J. Biol. Chem. 263:4641-4646(1988) [PubMed: 2832401] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Structure and probable genetic location of a 'ribosome modulation factor' associated with 100S ribosomes in stationary-phase Escherichia coli cells."
Wada A., Yamazaki Y., Fujita N., Ishihama A.
Proc. Natl. Acad. Sci. U.S.A. 87:2657-2661(1990) [PubMed: 2181444] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-30.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X70111 Genomic DNA. Translation: CAA49706.1.
J03186 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74039.1.
AP009048 Genomic DNA. Translation: BAA35711.1.
PIRH64835.
RefSeqNP_415473.1. NC_000913.2.

3D structure databases

ProteinModelPortalP0AFW2.
SMRP0AFW2. Positions 1-51.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-48260N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000002527; EBESCP00000002527; EBESCG00000002063.
EBESCT00000017954; EBESCP00000017245; EBESCG00000017010.
GeneID945567.
GenomeReviewsGene locus JW0936 in contig AP009048_GR.
Gene locus b0953 in contig U00096_GR.
KEGGecj:JW0936.
eco:b0953.
PATRIC32117125. VBIEscCol129921_0987.

Organism-specific databases

EchoBASEEB4298.
EcoGeneEG50004. rmf.

Phylogenomic databases

eggNOGCOG3130.
GeneTreeEBGT00050000011120.
HOGENOMHBG336266.
OMAWREAMED.
ProtClustDBPRK14563.

Enzyme and pathway databases

BioCycEcoCyc:EG50004-MONOMER.

Gene expression databases

GenevestigatorP0AFW2.

Family and domain databases

InterProIPR007040. Ribosome_modulation_factor.
IPR023200. Ribosome_modulation_factor_dom.
[Graphical view]
Gene3DG3DSA:1.10.10.620. G3DSA:1.10.10.620. 1 hit.
KOK03812.
PfamPF04957. RMF. 1 hit.
[Graphical view]
ProDomPD051650. Ribosome_modulation_factor. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameRMF_ECOLI
AccessionPrimary (citable) accession number: P0AFW2
Secondary accession number(s): P22986, P77441
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene