Reviewed, UniProtKB/Swiss-Prot P0AFW2 (RMF_ECOLI)

Names and origin

Protein names

Recommended name:
    Ribosome modulation factor
      Short name=Protein E

Gene names

Name:	rmf
Ordered Locus Names:	b0953, JW0936

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	55 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Associates with 70S ribosomes and converts them to a dimeric form (100S ribosomes) which appears during the transition from the exponential growth phase to the stationary phase of E.coli cells.
Sequence caution	The sequence J03186 differs from that shown. Reason: Frameshift at positions 4, 11 and 30.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ribosome biogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 55	55	Ribosome modulation factor		PRO_0000097363
Experimental info
	Sequence conflict	3	1	R → I AA sequence Ref.6
	Sequence conflict	25	1	S → V AA sequence Ref.6
	Sequence conflict	33	1	T → S Ref.1
	Sequence conflict	33	1	T → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0AFW2-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: D264AF285BBF24AB	FASTA	55	6,507
	10 20 30 40 50 MKRQKRDRLE RAHQRGYQAG IAGRSKEMCP YQTLNQRSQW LGGWREAMAD RVVMA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of the Escherichia coli rmf gene encoding the ribosome modulation factor: growth phase- and growth rate-dependent control." Yamagishi M., Matsushima H., Wada A., Sakagami M., Fujita N., Ishihama A. EMBO J. 12:625-630(1993) [PubMed: 8440252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Derived amino acid sequence and identification of active site residues of Escherichia coli beta-hydroxydecanoyl thioester dehydrase." Cronan J.E. Jr., Li W.-B., Coleman R., Narasimhan M., de Mendoza D., Schwab J.M. J. Biol. Chem. 263:4641-4646(1988) [PubMed: 2832401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Structure and probable genetic location of a 'ribosome modulation factor' associated with 100S ribosomes in stationary-phase Escherichia coli cells." Wada A., Yamazaki Y., Fujita N., Ishihama A. Proc. Natl. Acad. Sci. U.S.A. 87:2657-2661(1990) [PubMed: 2181444] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70111 Genomic DNA. Translation: CAA49706.1. J03186 Genomic DNA. No translation available. U00096 Genomic DNA. Translation: AAC74039.1. AP009048 Genomic DNA. Translation: BAA35711.1.
PIR	H64835.
RefSeq	AP_001583.1. NP_415473.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	945567.
GenomeReviews	Gene locus JW0936 in contig AP009048_GR. Gene locus b0953 in contig U00096_GR.
KEGG	ecj:JW0936. eco:b0953.
Organism-specific databases
EchoBASE	EB4298.
EcoGene	EG50004. rmf.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AFW2.
OMA	P0AFW2. DARSYWL.
Enzyme and pathway databases
BioCyc	EcoCyc:EG50004-MON.
Family and domain databases
InterPro	IPR007040. Ribosome_modulation_factor. [Graphical view]
Pfam	PF04957. RMF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RMF_ECOLI

Accession

Primary (citable) accession number: P0AFW2
Secondary accession number(s): P22986, P77441

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)