Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P0AFW2 (RMF_ECOLI)

Last modified June 16, 2009. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ribosome modulation factor
      Short name=Protein E
Gene names
Name: rmf
Ordered Locus Names: b0953, JW0936
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length55 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Associates with 70S ribosomes and converts them to a dimeric form (100S ribosomes) which appears during the transition from the exponential growth phase to the stationary phase of E.coli cells.

Sequence caution

The sequence J03186 differs from that shown. Reason: Frameshift at positions 4, 11 and 30.

Ontologies

Keywords
   Biological processRibosome biogenesis
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processribosome biogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5555Ribosome modulation factor
PRO_0000097363

Experimental info

Sequence conflict31R → I AA sequence Ref.6
Sequence conflict251S → V AA sequence Ref.6
Sequence conflict331T → S Ref.1
Sequence conflict331T → S Ref.2

Sequences

Sequence LengthMass (Da)Tools
P0AFW2-1 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: D264AF285BBF24AB

FASTA556,507
        10         20         30         40         50 
MKRQKRDRLE RAHQRGYQAG IAGRSKEMCP YQTLNQRSQW LGGWREAMAD RVVMA 

« Hide

References

« Hide 'large scale' references
[1]"Regulation of the Escherichia coli rmf gene encoding the ribosome modulation factor: growth phase- and growth rate-dependent control."
Yamagishi M., Matsushima H., Wada A., Sakagami M., Fujita N., Ishihama A.
EMBO J. 12:625-630(1993) [PubMed: 8440252] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Derived amino acid sequence and identification of active site residues of Escherichia coli beta-hydroxydecanoyl thioester dehydrase."
Cronan J.E. Jr., Li W.-B., Coleman R., Narasimhan M., de Mendoza D., Schwab J.M.
J. Biol. Chem. 263:4641-4646(1988) [PubMed: 2832401] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Structure and probable genetic location of a 'ribosome modulation factor' associated with 100S ribosomes in stationary-phase Escherichia coli cells."
Wada A., Yamazaki Y., Fujita N., Ishihama A.
Proc. Natl. Acad. Sci. U.S.A. 87:2657-2661(1990) [PubMed: 2181444] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-30.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

X70111 Genomic DNA. Translation: CAA49706.1.
J03186 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74039.1.
AP009048 Genomic DNA. Translation: BAA35711.1.
PIRH64835.
RefSeqAP_001583.1.
NP_415473.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID945567.
GenomeReviewsGene locus JW0936 in contig AP009048_GR.
Gene locus b0953 in contig U00096_GR.
KEGGecj:JW0936.
eco:b0953.

Organism-specific databases

EchoBASEEB4298.
EcoGeneEG50004. rmf.
CMRSearch...

Phylogenomic databases

HOGENOMP0AFW2.
OMAP0AFW2. DARSYWL.

Enzyme and pathway databases

BioCycEcoCyc:EG50004-MON.

Family and domain databases

InterProIPR007040. Ribosome_modulation_factor.
[Graphical view]
PfamPF04957. RMF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRMF_ECOLI
AccessionPrimary (citable) accession number: P0AFW2
Secondary accession number(s): P22986, P77441
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents