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Protein

Riboflavin synthase

Gene

ribC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.1 Publication

Catalytic activityi

2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.1 Publication

Pathwayi: riboflavin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 6,7-dimethyl-8-ribityllumazine synthase (ribE)
  2. Riboflavin synthase (ribC)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei137Substrate 2 bindingBy similarity1

GO - Molecular functioni

  • oxidoreductase activity Source: InterPro
  • riboflavin synthase activity Source: EcoCyc

GO - Biological processi

  • riboflavin biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Riboflavin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:RIBOFLAVIN-SYN-MONOMER.
ECOL316407:JW1654-MONOMER.
MetaCyc:RIBOFLAVIN-SYN-MONOMER.
BRENDAi2.5.1.9. 2026.
UniPathwayiUPA00275; UER00405.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin synthase (EC:2.5.1.9)
Short name:
RS
Gene namesi
Name:ribC
Synonyms:ribE
Ordered Locus Names:b1662, JW1654
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11406. ribC.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

DrugBankiDB00140. Riboflavin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000681661 – 213Riboflavin synthaseAdd BLAST213

Proteomic databases

EPDiP0AFU8.
PaxDbiP0AFU8.
PRIDEiP0AFU8.

2D gel databases

SWISS-2DPAGEP0AFU8.

Interactioni

Subunit structurei

Homotrimer. Unlike in B.subtilis, does not interact with 6,7-dimethyl-8-ribityllumazine synthase.3 Publications

Protein-protein interaction databases

DIPiDIP-47865N.
IntActiP0AFU8. 3 interactors.
STRINGi511145.b1662.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 17Combined sources10
Beta strandi19 – 27Combined sources9
Helixi30 – 32Combined sources3
Beta strandi41 – 44Combined sources4
Beta strandi47 – 55Combined sources9
Beta strandi58 – 64Combined sources7
Helixi65 – 70Combined sources6
Helixi72 – 75Combined sources4
Beta strandi81 – 86Combined sources6
Beta strandi105 – 115Combined sources11
Beta strandi118 – 126Combined sources9
Helixi128 – 133Combined sources6
Beta strandi139 – 142Combined sources4
Beta strandi145 – 148Combined sources4
Beta strandi154 – 161Combined sources8
Helixi163 – 168Combined sources6
Helixi171 – 173Combined sources3
Beta strandi179 – 184Combined sources6
Helixi186 – 204Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HZENMR-A/B1-97[»]
1I18NMR-A/B1-97[»]
1I8DX-ray2.00A/B/C1-213[»]
1PKVX-ray2.60A/B1-97[»]
ProteinModelPortaliP0AFU8.
SMRiP0AFU8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFU8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 97Lumazine-binding 1Add BLAST97
Repeati98 – 195Lumazine-binding 2Add BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 6Substrate 1 bindingBy similarity1 Publication3
Regioni48 – 50Substrate 2 binding; shared with one trimeric partnerBy similarity1 Publication3
Regioni62 – 67Substrate 2 binding; shared with one trimeric partnerBy similarity1 Publication6
Regioni101 – 103Substrate 2 binding; shared with one trimeric partnerBy similarity3
Regioni146 – 148Substrate 1 bindingBy similarity3
Regioni160 – 165Substrate 1 bindingBy similarity6

Sequence similaritiesi

Contains 2 lumazine-binding repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108R6K. Bacteria.
COG0307. LUCA.
HOGENOMiHOG000151758.
InParanoidiP0AFU8.
KOiK00793.
OMAiHILSGHV.
PhylomeDBiP0AFU8.

Family and domain databases

Gene3Di2.40.30.20. 2 hits.
InterProiIPR023366. ATP_synth_asu-like.
IPR001783. Lumazine-bd.
IPR026017. Lumazine-bd_dom.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR21098. PTHR21098. 1 hit.
PfamiPF00677. Lum_binding. 2 hits.
[Graphical view]
PIRSFiPIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAMiSSF63380. SSF63380. 2 hits.
TIGRFAMsiTIGR00187. ribE. 1 hit.
PROSITEiPS51177. LUMAZINE_BIND. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFU8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTGIVQGTA KLVSIDEKPN FRTHVVELPD HMLDGLETGA SVAHNGCCLT
60 70 80 90 100
VTEINGNHVS FDLMKETLRI TNLGDLKVGD WVNVERAAKF SDEIGGHLMS
110 120 130 140 150
GHIMTTAEVA KILTSENNRQ IWFKVQDSQL MKYILYKGFI GIDGISLTVG
160 170 180 190 200
EVTPTRFCVH LIPETLERTT LGKKKLGARV NIEIDPQTQA VVDTVERVLA
210
ARENAMNQPG TEA
Length:213
Mass (Da):23,445
Last modified:December 20, 2005 - v1
Checksum:iCD1C7E40E4AC88B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69109 Genomic DNA. Translation: CAA48861.1.
U68703 Genomic DNA. Translation: AAB47940.1.
U00096 Genomic DNA. Translation: AAC74734.1.
AP009048 Genomic DNA. Translation: BAA15429.1.
PIRiS28526.
RefSeqiNP_416179.1. NC_000913.3.
WP_000493947.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74734; AAC74734; b1662.
BAA15429; BAA15429; BAA15429.
GeneIDi945848.
KEGGiecj:JW1654.
eco:b1662.
PATRICi32118630. VBIEscCol129921_1735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69109 Genomic DNA. Translation: CAA48861.1.
U68703 Genomic DNA. Translation: AAB47940.1.
U00096 Genomic DNA. Translation: AAC74734.1.
AP009048 Genomic DNA. Translation: BAA15429.1.
PIRiS28526.
RefSeqiNP_416179.1. NC_000913.3.
WP_000493947.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HZENMR-A/B1-97[»]
1I18NMR-A/B1-97[»]
1I8DX-ray2.00A/B/C1-213[»]
1PKVX-ray2.60A/B1-97[»]
ProteinModelPortaliP0AFU8.
SMRiP0AFU8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47865N.
IntActiP0AFU8. 3 interactors.
STRINGi511145.b1662.

Chemistry databases

DrugBankiDB00140. Riboflavin.

2D gel databases

SWISS-2DPAGEP0AFU8.

Proteomic databases

EPDiP0AFU8.
PaxDbiP0AFU8.
PRIDEiP0AFU8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74734; AAC74734; b1662.
BAA15429; BAA15429; BAA15429.
GeneIDi945848.
KEGGiecj:JW1654.
eco:b1662.
PATRICi32118630. VBIEscCol129921_1735.

Organism-specific databases

EchoBASEiEB1378.
EcoGeneiEG11406. ribC.

Phylogenomic databases

eggNOGiENOG4108R6K. Bacteria.
COG0307. LUCA.
HOGENOMiHOG000151758.
InParanoidiP0AFU8.
KOiK00793.
OMAiHILSGHV.
PhylomeDBiP0AFU8.

Enzyme and pathway databases

UniPathwayiUPA00275; UER00405.
BioCyciEcoCyc:RIBOFLAVIN-SYN-MONOMER.
ECOL316407:JW1654-MONOMER.
MetaCyc:RIBOFLAVIN-SYN-MONOMER.
BRENDAi2.5.1.9. 2026.

Miscellaneous databases

EvolutionaryTraceiP0AFU8.
PROiP0AFU8.

Family and domain databases

Gene3Di2.40.30.20. 2 hits.
InterProiIPR023366. ATP_synth_asu-like.
IPR001783. Lumazine-bd.
IPR026017. Lumazine-bd_dom.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR21098. PTHR21098. 1 hit.
PfamiPF00677. Lum_binding. 2 hits.
[Graphical view]
PIRSFiPIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAMiSSF63380. SSF63380. 2 hits.
TIGRFAMsiTIGR00187. ribE. 1 hit.
PROSITEiPS51177. LUMAZINE_BIND. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRISA_ECOLI
AccessioniPrimary (citable) accession number: P0AFU8
Secondary accession number(s): P29015
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 30, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.