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P0AFU8

- RISA_ECOLI

UniProt

P0AFU8 - RISA_ECOLI

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Protein

Riboflavin synthase

Gene

ribC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.1 Publication

Catalytic activityi

2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.1 Publication

Pathwayi

GO - Molecular functioni

  1. oxidoreductase activity Source: InterPro
  2. riboflavin synthase activity Source: EcoCyc

GO - Biological processi

  1. riboflavin biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Riboflavin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:RIBOFLAVIN-SYN-MONOMER.
ECOL316407:JW1654-MONOMER.
MetaCyc:RIBOFLAVIN-SYN-MONOMER.
UniPathwayiUPA00275; UER00405.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin synthase (EC:2.5.1.9)
Short name:
RS
Gene namesi
Name:ribC
Synonyms:ribE
Ordered Locus Names:b1662, JW1654
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11406. ribC.

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Riboflavin synthasePRO_0000068166Add
BLAST

Proteomic databases

PaxDbiP0AFU8.
PRIDEiP0AFU8.

2D gel databases

SWISS-2DPAGEP0AFU8.

Expressioni

Gene expression databases

GenevestigatoriP0AFU8.

Interactioni

Subunit structurei

Homotrimer. Unlike in B.subtilis, does not interact with 6,7-dimethyl-8-ribityllumazine synthase.3 Publications

Protein-protein interaction databases

DIPiDIP-47865N.
IntActiP0AFU8. 3 interactions.
STRINGi511145.b1662.

Structurei

Secondary structure

1
213
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 1710Combined sources
Beta strandi19 – 279Combined sources
Helixi30 – 323Combined sources
Beta strandi41 – 444Combined sources
Beta strandi47 – 559Combined sources
Beta strandi58 – 647Combined sources
Helixi65 – 706Combined sources
Helixi72 – 754Combined sources
Beta strandi81 – 866Combined sources
Beta strandi105 – 11511Combined sources
Beta strandi118 – 1269Combined sources
Helixi128 – 1336Combined sources
Beta strandi139 – 1424Combined sources
Beta strandi145 – 1484Combined sources
Beta strandi154 – 1618Combined sources
Helixi163 – 1686Combined sources
Helixi171 – 1733Combined sources
Beta strandi179 – 1846Combined sources
Helixi186 – 20419Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HZENMR-A/B1-97[»]
1I18NMR-A/B1-97[»]
1I8DX-ray2.00A/B/C1-213[»]
1PKVX-ray2.60A/B1-97[»]
ProteinModelPortaliP0AFU8.
SMRiP0AFU8. Positions 1-206.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFU8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 9797Lumazine-binding 1Add
BLAST
Repeati98 – 19598Lumazine-binding 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 63Substrate binding
Regioni48 – 503Substrate binding
Regioni62 – 676Substrate binding

Sequence similaritiesi

Contains 2 lumazine-binding repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0307.
HOGENOMiHOG000151758.
InParanoidiP0AFU8.
KOiK00793.
OMAiNHQIWFR.
OrthoDBiEOG6VMTQH.
PhylomeDBiP0AFU8.

Family and domain databases

Gene3Di2.40.30.20. 2 hits.
InterProiIPR023366. ATPase_asu-like.
IPR001783. Lumazine-bd.
IPR026017. Lumazine-bd_dom.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR21098. PTHR21098. 1 hit.
PfamiPF00677. Lum_binding. 2 hits.
[Graphical view]
PIRSFiPIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAMiSSF63380. SSF63380. 2 hits.
TIGRFAMsiTIGR00187. ribE. 1 hit.
PROSITEiPS51177. LUMAZINE_BIND. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFU8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFTGIVQGTA KLVSIDEKPN FRTHVVELPD HMLDGLETGA SVAHNGCCLT
60 70 80 90 100
VTEINGNHVS FDLMKETLRI TNLGDLKVGD WVNVERAAKF SDEIGGHLMS
110 120 130 140 150
GHIMTTAEVA KILTSENNRQ IWFKVQDSQL MKYILYKGFI GIDGISLTVG
160 170 180 190 200
EVTPTRFCVH LIPETLERTT LGKKKLGARV NIEIDPQTQA VVDTVERVLA
210
ARENAMNQPG TEA
Length:213
Mass (Da):23,445
Last modified:December 20, 2005 - v1
Checksum:iCD1C7E40E4AC88B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69109 Genomic DNA. Translation: CAA48861.1.
U68703 Genomic DNA. Translation: AAB47940.1.
U00096 Genomic DNA. Translation: AAC74734.1.
AP009048 Genomic DNA. Translation: BAA15429.1.
PIRiS28526.
RefSeqiNP_416179.1. NC_000913.3.
YP_489926.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74734; AAC74734; b1662.
BAA15429; BAA15429; BAA15429.
GeneIDi12932660.
945848.
KEGGiecj:Y75_p1639.
eco:b1662.
PATRICi32118630. VBIEscCol129921_1735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69109 Genomic DNA. Translation: CAA48861.1 .
U68703 Genomic DNA. Translation: AAB47940.1 .
U00096 Genomic DNA. Translation: AAC74734.1 .
AP009048 Genomic DNA. Translation: BAA15429.1 .
PIRi S28526.
RefSeqi NP_416179.1. NC_000913.3.
YP_489926.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HZE NMR - A/B 1-97 [» ]
1I18 NMR - A/B 1-97 [» ]
1I8D X-ray 2.00 A/B/C 1-213 [» ]
1PKV X-ray 2.60 A/B 1-97 [» ]
ProteinModelPortali P0AFU8.
SMRi P0AFU8. Positions 1-206.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47865N.
IntActi P0AFU8. 3 interactions.
STRINGi 511145.b1662.

Chemistry

DrugBanki DB00140. Riboflavin.

2D gel databases

SWISS-2DPAGE P0AFU8.

Proteomic databases

PaxDbi P0AFU8.
PRIDEi P0AFU8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74734 ; AAC74734 ; b1662 .
BAA15429 ; BAA15429 ; BAA15429 .
GeneIDi 12932660.
945848.
KEGGi ecj:Y75_p1639.
eco:b1662.
PATRICi 32118630. VBIEscCol129921_1735.

Organism-specific databases

EchoBASEi EB1378.
EcoGenei EG11406. ribC.

Phylogenomic databases

eggNOGi COG0307.
HOGENOMi HOG000151758.
InParanoidi P0AFU8.
KOi K00793.
OMAi NHQIWFR.
OrthoDBi EOG6VMTQH.
PhylomeDBi P0AFU8.

Enzyme and pathway databases

UniPathwayi UPA00275 ; UER00405 .
BioCyci EcoCyc:RIBOFLAVIN-SYN-MONOMER.
ECOL316407:JW1654-MONOMER.
MetaCyc:RIBOFLAVIN-SYN-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AFU8.
PROi P0AFU8.

Gene expression databases

Genevestigatori P0AFU8.

Family and domain databases

Gene3Di 2.40.30.20. 2 hits.
InterProi IPR023366. ATPase_asu-like.
IPR001783. Lumazine-bd.
IPR026017. Lumazine-bd_dom.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
PANTHERi PTHR21098. PTHR21098. 1 hit.
Pfami PF00677. Lum_binding. 2 hits.
[Graphical view ]
PIRSFi PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAMi SSF63380. SSF63380. 2 hits.
TIGRFAMsi TIGR00187. ribE. 1 hit.
PROSITEi PS51177. LUMAZINE_BIND. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, mapping and hyperexpression of the ribC gene coding for riboflavin synthase of Escherichia coli."
    Eberhardt S.M.R., Richter G., Gimbel W., Werner T., Bacher A.
    Eur. J. Biochem. 242:712-719(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
    Strain: K12 / RR28.
  2. "Analysis of the boundaries of Salmonella pathogenicity island 2 and the corresponding chromosomal region of Escherichia coli K-12."
    Hensel M., Shea J.E., Baeumler A.J., Gleeson C., Blattner F.R., Holden D.W.
    J. Bacteriol. 179:1105-1111(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "The solution structure of the N-terminal domain of riboflavin synthase."
    Truffault V., Coles M., Diercks T., Abelmann K., Eberhardt S., Luttgen H., Bacher A., Kessler H.
    J. Mol. Biol. 309:949-960(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-97 IN COMPLEX WITH RIBOFLAVIN.
  7. Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  8. "The structure of the N-terminal domain of riboflavin synthase in complex with riboflavin at 2.6A resolution."
    Meining W., Eberhardt S., Bacher A., Ladenstein R.
    J. Mol. Biol. 331:1053-1063(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 1-97 IN COMPLEX WITH RIBOFLAVIN.

Entry informationi

Entry nameiRISA_ECOLI
AccessioniPrimary (citable) accession number: P0AFU8
Secondary accession number(s): P29015
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3