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Protein

Transcriptional regulatory protein GlrR

Gene

glrR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system GlrR/GlrK that up-regulates transcription of the glmY sRNA when cells enter the stationary growth phase. Regulates glmY transcription by binding to three conserved sites in the purL-glmY intergenic region.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 1718ATPPROSITE-ProRule annotation
Nucleotide bindingi227 – 23610ATPPROSITE-ProRule annotation
DNA bindingi414 – 43320H-T-H motifSequence analysisAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • phosphorelay response regulator activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11285-MONOMER.
ECOL316407:JW2538-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein GlrR
Gene namesi
Name:glrR
Synonyms:yfhA
Ordered Locus Names:b2554, JW2538
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11285. glrR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutants show decreased amounts of glmY.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Transcriptional regulatory protein GlrRPRO_0000081377Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 5614-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by GlrK.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AFU4.
PRIDEiP0AFU4.

Interactioni

Protein-protein interaction databases

BioGridi4259202. 1 interaction.
DIPiDIP-12044N.
IntActiP0AFU4. 9 interactions.
MINTiMINT-1317436.
STRINGi511145.b2554.

Structurei

3D structure databases

ProteinModelPortaliP0AFU4.
SMRiP0AFU4. Positions 7-434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 121115Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini136 – 366231Sigma-54 factor interactionPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058489.
InParanoidiP0AFU4.
KOiK07715.
OMAiHNKITEY.
PhylomeDBiP0AFU4.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011006. CheY-like_superfamily.
IPR009057. Homeodomain-like.
IPR027417. P-loop_NTPase.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHKPAHLLL VDDDPGLLKL LGLRLTSEGY SVVTAESGAE GLRVLNREKV
60 70 80 90 100
DLVISDLRMD EMDGMQLFAE IQKVQPGMPV IILTAHGSIP DAVAATQQGV
110 120 130 140 150
FSFLTKPVDK DALYQAIDDA LEQSAPATDE RWREAIVTRS PLMLRLLEQA
160 170 180 190 200
RLVAQSDVSV LINGQSGTGK EIFAQAIHNA SPRNSKPFIA INCGALPEQL
210 220 230 240 250
LESELFGHAR GAFTGAVSNR EGLFQAAEGG TLFLDEIGDM PAPLQVKLLR
260 270 280 290 300
VLQERKVRPL GSNRDIDINV RIISATHRDL PKAMARGEFR EDLYYRLNVV
310 320 330 340 350
SLKIPALAER TEDIPLLANH LLRQAAERHK PFVRAFSTDA MKRLMTASWP
360 370 380 390 400
GNVRQLVNVI EQCVALTSSP VISDALVEQA LEGENTALPT FVEARNQFEL
410 420 430 440
NYLRKLLQIT KGNVTHAARM AGRNRTEFYK LLSRHELDAN DFKE
Length:444
Mass (Da):49,148
Last modified:December 20, 2005 - v1
Checksum:i6553C027DE5513EF
GO

Sequence cautioni

The sequence AAA79816 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061K → N in AAB28778 (PubMed:8226691).Curated
Sequence conflicti309 – 3102ER → DG in AAB28778 (PubMed:8226691).Curated
Sequence conflicti327 – 3282ER → DG in AAB28778 (PubMed:8226691).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67014 Genomic DNA. Translation: AAB28778.1.
U00096 Genomic DNA. Translation: AAC75607.1.
AP009048 Genomic DNA. Translation: BAA16462.1.
Z21843 Genomic DNA. Translation: CAA79889.1.
M16778 Genomic DNA. No translation available.
U36841 Genomic DNA. Translation: AAA79816.1. Different initiation.
PIRiA65033.
RefSeqiNP_417049.1. NC_000913.3.
WP_001295369.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75607; AAC75607; b2554.
BAA16462; BAA16462; BAA16462.
GeneIDi947042.
KEGGiecj:JW2538.
eco:b2554.
PATRICi32120507. VBIEscCol129921_2656.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67014 Genomic DNA. Translation: AAB28778.1.
U00096 Genomic DNA. Translation: AAC75607.1.
AP009048 Genomic DNA. Translation: BAA16462.1.
Z21843 Genomic DNA. Translation: CAA79889.1.
M16778 Genomic DNA. No translation available.
U36841 Genomic DNA. Translation: AAA79816.1. Different initiation.
PIRiA65033.
RefSeqiNP_417049.1. NC_000913.3.
WP_001295369.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFU4.
SMRiP0AFU4. Positions 7-434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259202. 1 interaction.
DIPiDIP-12044N.
IntActiP0AFU4. 9 interactions.
MINTiMINT-1317436.
STRINGi511145.b2554.

Proteomic databases

PaxDbiP0AFU4.
PRIDEiP0AFU4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75607; AAC75607; b2554.
BAA16462; BAA16462; BAA16462.
GeneIDi947042.
KEGGiecj:JW2538.
eco:b2554.
PATRICi32120507. VBIEscCol129921_2656.

Organism-specific databases

EchoBASEiEB1262.
EcoGeneiEG11285. glrR.

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058489.
InParanoidiP0AFU4.
KOiK07715.
OMAiHNKITEY.
PhylomeDBiP0AFU4.

Enzyme and pathway databases

BioCyciEcoCyc:EG11285-MONOMER.
ECOL316407:JW2538-MONOMER.

Miscellaneous databases

PROiP0AFU4.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011006. CheY-like_superfamily.
IPR009057. Homeodomain-like.
IPR027417. P-loop_NTPase.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRR_ECOLI
AccessioniPrimary (citable) accession number: P0AFU4
Secondary accession number(s): P21712, P77512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Not required for the regulation of the glmY-glmZ-glmS regulatory cascade by glucosamine-6-phosphate depletion.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.