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Protein

Transcriptional regulatory protein BtsR

Gene

btsR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system BtsS/BtsR, which is part of a nutrient-sensing regulatory network composed of BtsS/BtsR, the low-affinity pyruvate signaling system YpdA/YpdB and their respective target proteins, YjiY and YhjX. Responds to depletion of nutrients, specifically serine, and the concomitant presence of extracellular pyruvate. BtsR regulates expression of yjiY by binding to its promoter region. Activation of the BtsS/BtsR signaling cascade also suppresses YpdA/YpdB-mediated yhjX induction.4 Publications

GO - Molecular functioni

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation, Two-component regulatory system

Enzyme and pathway databases

BioCyciEcoCyc:EG12006-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein BtsRCurated
Gene namesi
Name:btsR1 Publication
Synonyms:yehT
Ordered Locus Names:b2125, JW5352
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12006. yehT.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Deletion of btsSR has no obvious phenotypic effect under the conditions tested.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi54D → E: 2-fold increase in yjiY induction. 1 Publication1
Mutagenesisi54D → N: 10-fold decrease in yjiY induction. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000813611 – 239Transcriptional regulatory protein BtsRAdd BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei544-aspartylphosphatePROSITE-ProRule annotation1

Post-translational modificationi

Phosphorylated by BtsS.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AFT5.
PRIDEiP0AFT5.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
sfsAP0A8232EBI-556431,EBI-556413

Protein-protein interaction databases

BioGridi4261849. 319 interactors.
DIPiDIP-48085N.
IntActiP0AFT5. 3 interactors.
MINTiMINT-1311507.
STRINGi316385.ECDH10B_2280.

Structurei

3D structure databases

ProteinModelPortaliP0AFT5.
SMRiP0AFT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 116Response regulatoryPROSITE-ProRule annotationAdd BLAST114
Domaini137 – 239HTH LytTR-typePROSITE-ProRule annotationAdd BLAST103

Phylogenomic databases

eggNOGiENOG4108NEV. Bacteria.
COG3279. LUCA.
HOGENOMiHOG000242207.
InParanoidiP0AFT5.
KOiK02477.
PhylomeDBiP0AFT5.

Family and domain databases

InterProiView protein in InterPro
IPR011006. CheY-like_superfamily.
IPR007492. LytTR_DNA-bd_dom.
IPR001789. Sig_transdc_resp-reg_receiver.
PfamiView protein in Pfam
PF04397. LytTR. 1 hit.
PF00072. Response_reg. 1 hit.
SMARTiView protein in SMART
SM00850. LytTR. 1 hit.
SM00448. REC. 1 hit.
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiView protein in PROSITE
PS50930. HTH_LYTTR. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVLIVDDE PLARENLRVF LQEQSDIEIV GECSNAVEGI GAVHKLRPDV
60 70 80 90 100
LFLDIQMPRI SGLEMVGMLD PEHRPYIVFL TAFDEYAIKA FEEHAFDYLL
110 120 130 140 150
KPIDEARLEK TLARLRQERS KQDVSLLPEN QQALKFIPCT GHSRIYLLQM
160 170 180 190 200
KDVAFVSSRM SGVYVTSHEG KEGFTELTLR TLESRTPLLR CHRQYLVNLA
210 220 230
HLQEIRLEDN GQAELILRNG LTVPVSRRYL KSLKEAIGL
Length:239
Mass (Da):27,400
Last modified:December 20, 2005 - v1
Checksum:i5665AAE782B7FBA3
GO

Sequence cautioni

The sequence AAA60488 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60488.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75186.2.
AP009048 Genomic DNA. Translation: BAE76601.1.
PIRiD64980.
RefSeqiNP_416629.4. NC_000913.3.
WP_000598641.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75186; AAC75186; b2125.
BAE76601; BAE76601; BAE76601.
GeneIDi949024.
KEGGiecj:JW5352.
eco:b2125.
PATRICifig|1411691.4.peg.120.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiBTSR_ECOLI
AccessioniPrimary (citable) accession number: P0AFT5
Secondary accession number(s): P33356
, P76432, P76433, Q2MAV5, Q9ALR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: July 5, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene