Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-cystine transport system permease protein YecS

Gene

yecS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex FliY-YecC-YecS involved in L-cystine transport. The system can probably also transport L-cysteine, and it mediates accumulation of the toxic compounds L-selenaproline (SCA) and L-selenocystine (SeCys) (PubMed:25139244). Probably responsible for the translocation of the substrate across the membrane (Probable).Curated1 Publication

Enzyme regulationi

The FliY-YecC-YecS system is inhibited by L-cystine, L-cysteine, DL-2,6-diaminopimelic acid and L-cystathionine, and is stimulated by D-cysteine.1 Publication

GO - Molecular functioni

  • cysteine transmembrane transporter activity Source: EcoCyc
  • L-cystine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cysteine transport Source: EcoCyc
  • L-cystine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7037-MONOMER.
ECOL316407:JW1903-MONOMER.
MetaCyc:G7037-MONOMER.

Protein family/group databases

TCDBi3.A.1.3.10. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cystine transport system permease protein YecSCurated
Gene namesi
Name:yecS
Ordered Locus Names:b1918, JW1903
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14037. yecS.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22PeriplasmicCuratedAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 64CytoplasmicCuratedAdd BLAST21
Transmembranei65 – 85HelicalSequence analysisAdd BLAST21
Topological domaini86 – 182PeriplasmicCuratedAdd BLAST97
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 222Cytoplasmic1 PublicationAdd BLAST19

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

The yecS-ydjN double mutant is completely resistant to both L-selenaproline and L-selenocystine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000602501 – 222L-cystine transport system permease protein YecSAdd BLAST222

Proteomic databases

PaxDbiP0AFT2.
PRIDEiP0AFT2.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (YecC), two transmembrane proteins (YecS) and a solute-binding protein (FliY).Curated

Protein-protein interaction databases

BioGridi4261651. 11 interactors.
IntActiP0AFT2. 1 interactor.
STRINGi511145.b1918.

Structurei

3D structure databases

ProteinModelPortaliP0AFT2.
SMRiP0AFT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 207ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST189

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105E5Q. Bacteria.
COG0765. LUCA.
HOGENOMiHOG000267552.
InParanoidiP0AFT2.
KOiK10009.
OMAiVNWIARI.
PhylomeDBiP0AFT2.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR010065. AA_ABC_transptr_permease_3TM.
IPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
TIGRFAMsiTIGR01726. HEQRo_perm_3TM. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQESIQLVID SLPFLLKGAG YTLQLSIGGM FFGLLLGFIL ALMRLSPIWP
60 70 80 90 100
VRWLARFYIS IFRGTPLIAQ LFMIYYGLPQ FGIELDPIPS AMIGLSLNTA
110 120 130 140 150
AYAAETLRAA ISSIDKGQWE AAASIGMTPW QTMRRAILPQ AARVALPPLS
160 170 180 190 200
NSFISLVKDT SLAATIQVPE LFRQAQLITS RTLEVFTMYL AASLIYWIMA
210 220
TVLSTLQNHF ENQLNRQERE PK
Length:222
Mass (Da):24,801
Last modified:December 20, 2005 - v1
Checksum:iF926C4E00C5145D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74985.1.
AP009048 Genomic DNA. Translation: BAA15738.1.
PIRiC64955.
RefSeqiNP_416428.1. NC_000913.3.
WP_001158220.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74985; AAC74985; b1918.
BAA15738; BAA15738; BAA15738.
GeneIDi949105.
KEGGiecj:JW1903.
eco:b1918.
PATRICi32119171. VBIEscCol129921_2000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74985.1.
AP009048 Genomic DNA. Translation: BAA15738.1.
PIRiC64955.
RefSeqiNP_416428.1. NC_000913.3.
WP_001158220.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFT2.
SMRiP0AFT2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261651. 11 interactors.
IntActiP0AFT2. 1 interactor.
STRINGi511145.b1918.

Protein family/group databases

TCDBi3.A.1.3.10. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP0AFT2.
PRIDEiP0AFT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74985; AAC74985; b1918.
BAA15738; BAA15738; BAA15738.
GeneIDi949105.
KEGGiecj:JW1903.
eco:b1918.
PATRICi32119171. VBIEscCol129921_2000.

Organism-specific databases

EchoBASEiEB3791.
EcoGeneiEG14037. yecS.

Phylogenomic databases

eggNOGiENOG4105E5Q. Bacteria.
COG0765. LUCA.
HOGENOMiHOG000267552.
InParanoidiP0AFT2.
KOiK10009.
OMAiVNWIARI.
PhylomeDBiP0AFT2.

Enzyme and pathway databases

BioCyciEcoCyc:G7037-MONOMER.
ECOL316407:JW1903-MONOMER.
MetaCyc:G7037-MONOMER.

Miscellaneous databases

PROiP0AFT2.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR010065. AA_ABC_transptr_permease_3TM.
IPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
TIGRFAMsiTIGR01726. HEQRo_perm_3TM. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYECS_ECOLI
AccessioniPrimary (citable) accession number: P0AFT2
Secondary accession number(s): O07985, P76315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.