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P0AFS9

- MEPM_ECOLI

UniProt

P0AFS9 - MEPM_ECOLI

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Protein

Murein DD-endopeptidase MepM

Gene
mepM, yebA, b1856, JW5304
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

A murein DD-endopeptidase with specificity for D-Ala-meso-diaminopimelic acid (mDAP) cross-links. Its role is probably to cleave D-Ala-mDAP cross-links to allow insertion of new glycans and thus cell wall expansion. Functionally redundant with MepM and MepH. Partially suppresses an mepS disruption mutant.1 Publication

Cofactori

Zinc, although calcium also allows some activity.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi314 – 3141Zinc Reviewed prediction

GO - Molecular functioni

  1. endopeptidase activity Source: CACAO
  2. metal ion binding Source: UniProtKB-KW
  3. metalloendopeptidase activity Source: EcoCyc

GO - Biological processi

  1. autolysis Source: EcoliWiki
  2. capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
  3. cytokinetic cell separation Source: EcoliWiki
  4. peptidoglycan metabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10013-MONOMER.
ECOL316407:JW5304-MONOMER.
MetaCyc:EG10013-MONOMER.
UniPathwayiUPA00963.

Names & Taxonomyi

Protein namesi
Recommended name:
Murein DD-endopeptidase MepM (EC:3.4.24.-)
Alternative name(s):
Murein hydrolase MepM
ORFU
Gene namesi
Name:mepM
Synonyms:yebA
Ordered Locus Names:b1856, JW5304
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10013. mepM.

Subcellular locationi

Cell inner membrane; Single-pass membrane protein Reviewed prediction
Note: Uniform peripheral location, with partial enrichment at cell poles.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei21 – 4020Helical; Reviewed predictionAdd
BLAST
Topological domaini41 – 440400Periplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells are shorter in a single mutant, while triple envC-nlpD-mepM disruptions have defects in septation and cell separation and form long filaments (15-fold longer) and further yet by the quadruple disruption mutant (envC-nlpD-mepM-ygeR, over 21-fold longer). Quadruple mutants are less sensitive to ampicillin lysis (1 Publication). A triple mepS-mepH-mepM mutant is inviable, whereas a double mepS-mepM will grow on a nutrient-poor medium but not on a rich medium, suggesting the 3 endopeptidases are functionally redundant in vivo. Depletion experiments of the double or triple mutants lead to cell lysis, as well as significantly decreased incorporation of mDAP into peptidogylcan sacculi and increased amounts of the enzyme's substrate (Tetra-Tetra-anhydro muropeptide) (1 Publication).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Murein DD-endopeptidase MepMPRO_0000026826Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP0AFS9.

Interactioni

Protein-protein interaction databases

STRINGi511145.b1856.

Structurei

3D structure databases

ProteinModelPortaliP0AFS9.
SMRiP0AFS9. Positions 98-413.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati98 – 14245LysMAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M23B family.
Contains 1 LysM repeat.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0739.
HOGENOMiHOG000281149.
KOiK01417.
OMAiSSVLNQY.
OrthoDBiEOG61KBD1.
PhylomeDBiP0AFS9.

Family and domain databases

Gene3Di3.10.350.10. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018392. LysM_dom.
IPR013731. OapA_N.
IPR016047. Peptidase_M23.
[Graphical view]
PfamiPF01476. LysM. 1 hit.
PF08525. OapA_N. 1 hit.
PF01551. Peptidase_M23. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF54106. SSF54106. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFS9-1 [UniParc]FASTAAdd to Basket

« Hide

MQQIARSVAL AFNNLPRPHR VMLGSLTVLT LAVAVWRPYV YHRDATPIVK    50
TIELEQNEIR SLLPEASEPI DQAAQEDEAI PQDELDDKIA GEAGVHEYVV 100
STGDTLSSIL NQYGIDMGDI TQLAAADKEL RNLKIGQQLS WTLTADGELQ 150
RLTWEVSRRE TRTYDRTAAN GFKMTSEMQQ GEWVNNLLKG TVGGSFVASA 200
RNAGLTSAEV SAVIKAMQWQ MDFRKLKKGD EFAVLMSREM LDGKREQSQL 250
LGVRLRSEGK DYYAIRAEDG KFYDRNGTGL AKGFLRFPTA KQFRISSNFN 300
PRRTNPVTGR VAPHRGVDFA MPQGTPVLSV GDGEVVVAKR SGAAGYYVAI 350
RHGRSYTTRY MHLRKILVKP GQKVKRGDRI ALSGNTGRST GPHLHYEVWI 400
NQQAVNPLTA KLPRTEGLTG SDRREFLAQA KEIVPQLRFD 440
Length:440
Mass (Da):49,058
Last modified:December 20, 2005 - v1
Checksum:i30431410A5B1A7F7
GO

Sequence cautioni

The sequence AAA81029.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901A → P in AAA81029. 1 Publication
Sequence conflicti93 – 931A → T in AAA81029. 1 Publication
Sequence conflicti121 – 1211T → S in AAA81029. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74926.2.
AP009048 Genomic DNA. Translation: BAA15664.2.
U38702 Genomic DNA. Translation: AAA81029.1. Different initiation.
M77039 Genomic DNA. Translation: AAA24180.1.
RefSeqiNP_416370.2. NC_000913.3.
YP_490118.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74926; AAC74926; b1856.
BAA15664; BAA15664; BAA15664.
GeneIDi12931358.
946376.
KEGGiecj:Y75_p1832.
eco:b1856.
PATRICi32119035. VBIEscCol129921_1935.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74926.2 .
AP009048 Genomic DNA. Translation: BAA15664.2 .
U38702 Genomic DNA. Translation: AAA81029.1 . Different initiation.
M77039 Genomic DNA. Translation: AAA24180.1 .
RefSeqi NP_416370.2. NC_000913.3.
YP_490118.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AFS9.
SMRi P0AFS9. Positions 98-413.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b1856.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74926 ; AAC74926 ; b1856 .
BAA15664 ; BAA15664 ; BAA15664 .
GeneIDi 12931358.
946376.
KEGGi ecj:Y75_p1832.
eco:b1856.
PATRICi 32119035. VBIEscCol129921_1935.

Organism-specific databases

EchoBASEi EB0013.
EcoGenei EG10013. mepM.

Phylogenomic databases

eggNOGi COG0739.
HOGENOMi HOG000281149.
KOi K01417.
OMAi SSVLNQY.
OrthoDBi EOG61KBD1.
PhylomeDBi P0AFS9.

Enzyme and pathway databases

UniPathwayi UPA00963 .
BioCyci EcoCyc:EG10013-MONOMER.
ECOL316407:JW5304-MONOMER.
MetaCyc:EG10013-MONOMER.

Miscellaneous databases

PROi P0AFS9.

Gene expression databases

Genevestigatori P0AFS9.

Family and domain databases

Gene3Di 3.10.350.10. 1 hit.
InterProi IPR011055. Dup_hybrid_motif.
IPR018392. LysM_dom.
IPR013731. OapA_N.
IPR016047. Peptidase_M23.
[Graphical view ]
Pfami PF01476. LysM. 1 hit.
PF08525. OapA_N. 1 hit.
PF01551. Peptidase_M23. 1 hit.
[Graphical view ]
SMARTi SM00257. LysM. 1 hit.
[Graphical view ]
SUPFAMi SSF51261. SSF51261. 1 hit.
SSF54106. SSF54106. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Robison K., O'Keeffe T., Church G.M.
    Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-182.
    Strain: K12 / EMG2.
  5. "Isolation and characterization of the Escherichia coli msbB gene, a multicopy suppressor of null mutations in the high-temperature requirement gene htrB."
    Karow M.L., Georgopoulos C.
    J. Bacteriol. 174:702-710(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 149-440.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "LytM-domain factors are required for daughter cell separation and rapid ampicillin-induced lysis in Escherichia coli."
    Uehara T., Dinh T., Bernhardt T.G.
    J. Bacteriol. 191:5094-5107(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / TB28.
  7. "Three redundant murein endopeptidases catalyse an essential cleavage step in peptidoglycan synthesis of Escherichia coli K12."
    Singh S.K., SaiSree L., Amrutha R.N., Reddy M.
    Mol. Microbiol. 86:1036-1051(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A MUREIN DD-ENDOPEPTIDASE, COFACTOR, DISRUPTION PHENOTYPE.
    Strain: K12.

Entry informationi

Entry nameiMEPM_ECOLI
AccessioniPrimary (citable) accession number: P0AFS9
Secondary accession number(s): O07981, P24204, P76283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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