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Protein

Murein DD-endopeptidase MepM

Gene

mepM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A murein DD-endopeptidase with specificity for D-Ala-meso-diaminopimelic acid (mDAP) cross-links. Its role is probably to cleave D-Ala-mDAP cross-links to allow insertion of new glycans and thus cell wall expansion. Functionally redundant with MepM and MepH. Partially suppresses an mepS disruption mutant.1 Publication

Cofactori

Zn2+1 Publication, Ca2+1 PublicationNote: Zinc, although calcium also allows some activity.1 Publication

Pathwayi: cell wall polysaccharide biosynthesis

This protein is involved in the pathway cell wall polysaccharide biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway cell wall polysaccharide biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi314ZincSequence analysis1

GO - Molecular functioni

  • endopeptidase activity Source: CACAO
  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: EcoCyc

GO - Biological processi

  • autolysis Source: EcoliWiki
  • capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
  • cell separation after cytokinesis Source: EcoliWiki
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan metabolic process Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processCell wall biogenesis/degradation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10013-MONOMER
MetaCyc:EG10013-MONOMER
UniPathwayiUPA00963

Names & Taxonomyi

Protein namesi
Recommended name:
Murein DD-endopeptidase MepM (EC:3.4.24.-)
Alternative name(s):
Murein hydrolase MepM
ORFU
Gene namesi
Name:mepM
Synonyms:yebA
Ordered Locus Names:b1856, JW5304
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10013 mepM

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicSequence analysisAdd BLAST20
Transmembranei21 – 40HelicalSequence analysisAdd BLAST20
Topological domaini41 – 440PeriplasmicSequence analysisAdd BLAST400

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells are shorter in a single mutant, while triple envC-nlpD-mepM disruptions have defects in septation and cell separation and form long filaments (15-fold longer) and further yet by the quadruple disruption mutant (envC-nlpD-mepM-ygeR, over 21-fold longer). Quadruple mutants are less sensitive to ampicillin lysis (PubMed:19525345). A triple mepS-mepH-mepM mutant is inviable, whereas a double mepS-mepM will grow on a nutrient-poor medium but not on a rich medium, suggesting the 3 endopeptidases are functionally redundant in vivo. Depletion experiments of the double or triple mutants lead to cell lysis, as well as significantly decreased incorporation of mDAP into peptidogylcan sacculi and increased amounts of the enzyme's substrate (Tetra-Tetra-anhydro muropeptide) (PubMed:23062283).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000268261 – 440Murein DD-endopeptidase MepMAdd BLAST440

Proteomic databases

PaxDbiP0AFS9
PRIDEiP0AFS9

Interactioni

Protein-protein interaction databases

BioGridi4260352, 385 interactors
STRINGi316385.ECDH10B_1997

Structurei

3D structure databases

ProteinModelPortaliP0AFS9
SMRiP0AFS9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini96 – 141LysMPROSITE-ProRule annotationAdd BLAST46

Sequence similaritiesi

Belongs to the peptidase M23B family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DR5 Bacteria
COG0739 LUCA
HOGENOMiHOG000281149
InParanoidiP0AFS9
KOiK19304
OMAiGPHVCYR
PhylomeDBiP0AFS9

Family and domain databases

CDDicd00118 LysM, 1 hit
InterProiView protein in InterPro
IPR011055 Dup_hybrid_motif
IPR018392 LysM_dom
IPR013731 OapA_N
IPR016047 Peptidase_M23
PfamiView protein in Pfam
PF01476 LysM, 1 hit
PF08525 OapA_N, 1 hit
PF01551 Peptidase_M23, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 1 hit
SUPFAMiSSF51261 SSF51261, 1 hit
PROSITEiView protein in PROSITE
PS51782 LYSM, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQIARSVAL AFNNLPRPHR VMLGSLTVLT LAVAVWRPYV YHRDATPIVK
60 70 80 90 100
TIELEQNEIR SLLPEASEPI DQAAQEDEAI PQDELDDKIA GEAGVHEYVV
110 120 130 140 150
STGDTLSSIL NQYGIDMGDI TQLAAADKEL RNLKIGQQLS WTLTADGELQ
160 170 180 190 200
RLTWEVSRRE TRTYDRTAAN GFKMTSEMQQ GEWVNNLLKG TVGGSFVASA
210 220 230 240 250
RNAGLTSAEV SAVIKAMQWQ MDFRKLKKGD EFAVLMSREM LDGKREQSQL
260 270 280 290 300
LGVRLRSEGK DYYAIRAEDG KFYDRNGTGL AKGFLRFPTA KQFRISSNFN
310 320 330 340 350
PRRTNPVTGR VAPHRGVDFA MPQGTPVLSV GDGEVVVAKR SGAAGYYVAI
360 370 380 390 400
RHGRSYTTRY MHLRKILVKP GQKVKRGDRI ALSGNTGRST GPHLHYEVWI
410 420 430 440
NQQAVNPLTA KLPRTEGLTG SDRREFLAQA KEIVPQLRFD
Length:440
Mass (Da):49,058
Last modified:December 20, 2005 - v1
Checksum:i30431410A5B1A7F7
GO

Sequence cautioni

The sequence AAA81029 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90A → P in AAA81029 (Ref. 4) Curated1
Sequence conflicti93A → T in AAA81029 (Ref. 4) Curated1
Sequence conflicti121T → S in AAA81029 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74926.2
AP009048 Genomic DNA Translation: BAA15664.2
U38702 Genomic DNA Translation: AAA81029.1 Different initiation.
M77039 Genomic DNA Translation: AAA24180.1
RefSeqiNP_416370.2, NC_000913.3
WP_001184045.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74926; AAC74926; b1856
BAA15664; BAA15664; BAA15664
GeneIDi946376
KEGGiecj:JW5304
eco:b1856
PATRICifig|1411691.4.peg.392

Similar proteinsi

Entry informationi

Entry nameiMEPM_ECOLI
AccessioniPrimary (citable) accession number: P0AFS9
Secondary accession number(s): O07981, P24204, P76283
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families
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