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Protein

AI-2 transport protein TqsA

Gene

tqsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Controls the transport of the quorum-sensing signal AI-2 either by enhancing its secretion or inhibiting its uptake and consequently represses biofilm formation and motility and affects the global gene expression in biofilms.1 Publication

GO - Molecular functioni

  • efflux transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • quorum sensing Source: EcoCyc
  • single-species biofilm formation Source: EcoliWiki
  • transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Quorum sensing, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6859-MONOMER.
ECOL316407:JW1593-MONOMER.
MetaCyc:G6859-MONOMER.

Protein family/group databases

TCDBi2.A.86.1.4. the autoinducer-2 exporter (ai-2e) family (formerly the perm family, tc #9.b.22).

Names & Taxonomyi

Protein namesi
Recommended name:
AI-2 transport protein TqsA
Alternative name(s):
Transport of quorum-sensing signal protein
Gene namesi
Name:tqsA
Synonyms:ydgG
Ordered Locus Names:b1601, JW1593
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13928. tqsA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4PeriplasmicSequence analysis4
Transmembranei5 – 25HelicalSequence analysisAdd BLAST21
Topological domaini26 – 30CytoplasmicSequence analysis5
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Topological domaini52 – 61PeriplasmicSequence analysis10
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Topological domaini83 – 149CytoplasmicSequence analysisAdd BLAST67
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Topological domaini171 – 197PeriplasmicSequence analysisAdd BLAST27
Transmembranei198 – 218HelicalSequence analysisAdd BLAST21
Topological domaini219 – 221CytoplasmicSequence analysis3
Transmembranei222 – 242HelicalSequence analysisAdd BLAST21
Topological domaini243 – 257PeriplasmicSequence analysisAdd BLAST15
Transmembranei258 – 278HelicalSequence analysisAdd BLAST21
Topological domaini279 – 292CytoplasmicSequence analysisAdd BLAST14
Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
Topological domaini314 – 316PeriplasmicSequence analysis3
Transmembranei317 – 337HelicalSequence analysisAdd BLAST21
Topological domaini338 – 344CytoplasmicSequence analysis7

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells have a decreased extracellular and increased intracellular concentration of AI-2. Deletion of tqsA results in a 7000-fold increase in biofilm thickness and 574-fold increase in biomass in flow cells. Deletion of tqsA increases cell motility by increasing transcription of flagellar genes. The tqsA deletion mutant shows higher resistance toward crystal violet, spectinomycin, streptomycin sulfate, 2,6-dichloroquinone-4-chloroimide, chloramphenicol and amoxicillin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001482971 – 344AI-2 transport protein TqsAAdd BLAST344

Proteomic databases

PaxDbiP0AFS5.
PRIDEiP0AFS5.

Interactioni

Protein-protein interaction databases

BioGridi4260647. 14 interactors.
STRINGi511145.b1601.

Structurei

3D structure databases

ProteinModelPortaliP0AFS5.
SMRiP0AFS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UPF0118 (PerM) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DSF. Bacteria.
COG0628. LUCA.
HOGENOMiHOG000290196.
InParanoidiP0AFS5.
KOiK11744.
OMAiGMANIQR.
PhylomeDBiP0AFS5.

Family and domain databases

InterProiIPR002549. AI-2E-like.
[Graphical view]
PANTHERiPTHR21716. PTHR21716. 1 hit.
PfamiPF01594. AI-2E_transport. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKPIITLNG LKIVIMLGML VIILCGIRFA AEIIVPFILA LFIAVILNPL
60 70 80 90 100
VQHMVRWRVP RVLAVSILMT IIVMAMVLLL AYLGSALNEL TRTLPQYRNS
110 120 130 140 150
IMTPLQALEP LLQRVGIDVS VDQLAHYIDP NAAMTLLTNL LTQLSNAMSS
160 170 180 190 200
IFLLLLTVLF MLLEVPQLPG KFQQMMARPV EGMAAIQRAI DSVSHYLVLK
210 220 230 240 250
TAISIITGLV AWAMLAALDV RFAFVWGLLA FALNYIPNIG SVLAAIPPIA
260 270 280 290 300
QVLVFNGFYE ALLVLAGYLL INLVFGNILE PRIMGRGLGL STLVVFLSLI
310 320 330 340
FWGWLLGPVG MLLSVPLTII VKIALEQTAG GQSIAVLLSD LNKE
Length:344
Mass (Da):37,543
Last modified:December 20, 2005 - v1
Checksum:i9DFFAFCD1480C482
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74673.1.
AP009048 Genomic DNA. Translation: BAA15335.1.
PIRiC64916.
RefSeqiNP_416118.1. NC_000913.3.
WP_001118241.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74673; AAC74673; b1601.
BAA15335; BAA15335; BAA15335.
GeneIDi946142.
KEGGiecj:JW1593.
eco:b1601.
PATRICi32118504. VBIEscCol129921_1672.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74673.1.
AP009048 Genomic DNA. Translation: BAA15335.1.
PIRiC64916.
RefSeqiNP_416118.1. NC_000913.3.
WP_001118241.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFS5.
SMRiP0AFS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260647. 14 interactors.
STRINGi511145.b1601.

Protein family/group databases

TCDBi2.A.86.1.4. the autoinducer-2 exporter (ai-2e) family (formerly the perm family, tc #9.b.22).

Proteomic databases

PaxDbiP0AFS5.
PRIDEiP0AFS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74673; AAC74673; b1601.
BAA15335; BAA15335; BAA15335.
GeneIDi946142.
KEGGiecj:JW1593.
eco:b1601.
PATRICi32118504. VBIEscCol129921_1672.

Organism-specific databases

EchoBASEiEB3687.
EcoGeneiEG13928. tqsA.

Phylogenomic databases

eggNOGiENOG4105DSF. Bacteria.
COG0628. LUCA.
HOGENOMiHOG000290196.
InParanoidiP0AFS5.
KOiK11744.
OMAiGMANIQR.
PhylomeDBiP0AFS5.

Enzyme and pathway databases

BioCyciEcoCyc:G6859-MONOMER.
ECOL316407:JW1593-MONOMER.
MetaCyc:G6859-MONOMER.

Miscellaneous databases

PROiP0AFS5.

Family and domain databases

InterProiIPR002549. AI-2E-like.
[Graphical view]
PANTHERiPTHR21716. PTHR21716. 1 hit.
PfamiPF01594. AI-2E_transport. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTQSA_ECOLI
AccessioniPrimary (citable) accession number: P0AFS5
Secondary accession number(s): P77535
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.