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P0AFS4

- FOLM_ECO57

UniProt

P0AFS4 - FOLM_ECO57

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Protein

Dihydrofolate reductase FolM

Gene

folM

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reduction of dihydrofolate to tetrahydrofolate.By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. one-carbon metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciECOL386585:GJFA-2286-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase FolM (EC:1.5.1.3)
Short name:
DHFR
Gene namesi
Name:folM
Ordered Locus Names:Z2606, ECs2312
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Dihydrofolate reductase FolMPRO_0000054834Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi155864.Z2606.

Structurei

3D structure databases

ProteinModelPortaliP0AFS4.
SMRiP0AFS4. Positions 8-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK13938.
OMAiRRDIAPC.
OrthoDBiEOG6WDSG3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFS4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA
60 70 80 90 100
GAQCIQADFS TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLAD
110 120 130 140 150
VLACMMQIHV NTPYLLNHAL ERLLRGHGHA ASDIIHFTDY VVERGSDKHI
160 170 180 190 200
AYAASKAALD NMTRSFARKL APEVKVNSIA PSLILFNEHD DAEYRQQALN
210 220 230 240
KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR
Length:240
Mass (Da):26,348
Last modified:December 20, 2005 - v1
Checksum:i73B942EE144E2CC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56593.1.
BA000007 Genomic DNA. Translation: BAB35735.1.
PIRiE85766.
H90917.
RefSeqiNP_288041.1. NC_002655.2.
NP_310339.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG56593; AAG56593; Z2606.
BAB35735; BAB35735; BAB35735.
GeneIDi916931.
961555.
KEGGiece:Z2606.
ecs:ECs2312.
PATRICi18353952. VBIEscCol44059_2189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56593.1 .
BA000007 Genomic DNA. Translation: BAB35735.1 .
PIRi E85766.
H90917.
RefSeqi NP_288041.1. NC_002655.2.
NP_310339.1. NC_002695.1.

3D structure databases

ProteinModelPortali P0AFS4.
SMRi P0AFS4. Positions 8-240.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z2606.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG56593 ; AAG56593 ; Z2606 .
BAB35735 ; BAB35735 ; BAB35735 .
GeneIDi 916931.
961555.
KEGGi ece:Z2606.
ecs:ECs2312.
PATRICi 18353952. VBIEscCol44059_2189.

Phylogenomic databases

eggNOGi COG1028.
KOi K13938.
OMAi RRDIAPC.
OrthoDBi EOG6WDSG3.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-2286-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiFOLM_ECO57
AccessioniPrimary (citable) accession number: P0AFS4
Secondary accession number(s): P52109, P77386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 1, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3