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P0AFS4

- FOLM_ECO57

UniProt

P0AFS4 - FOLM_ECO57

Protein

Dihydrofolate reductase FolM

Gene

folM

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of dihydrofolate to tetrahydrofolate.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei152 – 1521Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. one-carbon metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-2286-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase FolM (EC:1.5.1.3)
    Short name:
    DHFR
    Gene namesi
    Name:folM
    Ordered Locus Names:Z2606, ECs2312
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 240240Dihydrofolate reductase FolMPRO_0000054834Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi155864.Z2606.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AFS4.
    SMRiP0AFS4. Positions 8-240.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    KOiK13938.
    OMAiRRDIAPC.
    OrthoDBiEOG6WDSG3.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AFS4-1 [UniParc]FASTAAdd to Basket

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    MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA    50
    GAQCIQADFS TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLAD 100
    VLACMMQIHV NTPYLLNHAL ERLLRGHGHA ASDIIHFTDY VVERGSDKHI 150
    AYAASKAALD NMTRSFARKL APEVKVNSIA PSLILFNEHD DAEYRQQALN 200
    KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR 240
    Length:240
    Mass (Da):26,348
    Last modified:December 20, 2005 - v1
    Checksum:i73B942EE144E2CC3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG56593.1.
    BA000007 Genomic DNA. Translation: BAB35735.1.
    PIRiE85766.
    H90917.
    RefSeqiNP_288041.1. NC_002655.2.
    NP_310339.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG56593; AAG56593; Z2606.
    BAB35735; BAB35735; BAB35735.
    GeneIDi916931.
    961555.
    KEGGiece:Z2606.
    ecs:ECs2312.
    PATRICi18353952. VBIEscCol44059_2189.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG56593.1 .
    BA000007 Genomic DNA. Translation: BAB35735.1 .
    PIRi E85766.
    H90917.
    RefSeqi NP_288041.1. NC_002655.2.
    NP_310339.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali P0AFS4.
    SMRi P0AFS4. Positions 8-240.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z2606.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG56593 ; AAG56593 ; Z2606 .
    BAB35735 ; BAB35735 ; BAB35735 .
    GeneIDi 916931.
    961555.
    KEGGi ece:Z2606.
    ecs:ECs2312.
    PATRICi 18353952. VBIEscCol44059_2189.

    Phylogenomic databases

    eggNOGi COG1028.
    KOi K13938.
    OMAi RRDIAPC.
    OrthoDBi EOG6WDSG3.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-2286-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiFOLM_ECO57
    AccessioniPrimary (citable) accession number: P0AFS4
    Secondary accession number(s): P52109, P77386
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3