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Protein

Dihydrofolate reductase FolM

Gene

folM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of dihydrofolate to tetrahydrofolate.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.1 Publication

Enzyme regulationi

Inhibited by methotrexate.1 Publication

Kineticsi

  1. KM=9.5 µM for dihydrofolate (at pH 6.0)1 Publication
  2. KM=1.9 µM for NADPH (at pH 6.0)1 Publication

Vmax=0.083 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 4.7.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. dihydromonapterin reductase activity Source: EcoCyc

GO - Biological processi

  1. 10-formyltetrahydrofolate biosynthetic process Source: EcoCyc
  2. folic acid biosynthetic process Source: EcoCyc
  3. one-carbon metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6862-MONOMER.
ECOL316407:JW1598-MONOMER.
MetaCyc:G6862-MONOMER.
SABIO-RKP0AFS3.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase FolM (EC:1.5.1.3)
Short name:
DHFR
Gene namesi
Name:folM
Synonyms:ydgB
Ordered Locus Names:b1606, JW1598
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13189. folM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Dihydrofolate reductase FolMPRO_0000054833Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP0AFS3.

Interactioni

Protein-protein interaction databases

DIPiDIP-47981N.
IntActiP0AFS3. 5 interactions.
STRINGi511145.b1606.

Structurei

3D structure databases

ProteinModelPortaliP0AFS3.
SMRiP0AFS3. Positions 8-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiP0AFS3.
KOiK13938.
OMAiAVIHNAS.
OrthoDBiEOG6WDSG3.
PhylomeDBiP0AFS3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFS3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA
60 70 80 90 100
GAQCIQADFS TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLAD
110 120 130 140 150
VLACMMQIHV NTPYLLNHAL ERLLRGHGHA ASDIIHFTDY VVERGSDKHI
160 170 180 190 200
AYAASKAALD NMTRSFARKL APEVKVNSIA PSLILFNEHD DAEYRQQALN
210 220 230 240
KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR
Length:240
Mass (Da):26,348
Last modified:December 20, 2005 - v1
Checksum:i73B942EE144E2CC3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti210 – 2101E → Q1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74678.1.
AP009048 Genomic DNA. Translation: BAA15344.1.
U41101 Genomic DNA. No translation available.
PIRiH64916.
RefSeqiNP_416123.1. NC_000913.3.
YP_489869.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74678; AAC74678; b1606.
BAA15344; BAA15344; BAA15344.
GeneIDi12933129.
949096.
KEGGiecj:Y75_p1582.
eco:b1606.
PATRICi32118514. VBIEscCol129921_1677.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74678.1.
AP009048 Genomic DNA. Translation: BAA15344.1.
U41101 Genomic DNA. No translation available.
PIRiH64916.
RefSeqiNP_416123.1. NC_000913.3.
YP_489869.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP0AFS3.
SMRiP0AFS3. Positions 8-240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47981N.
IntActiP0AFS3. 5 interactions.
STRINGi511145.b1606.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74678; AAC74678; b1606.
BAA15344; BAA15344; BAA15344.
GeneIDi12933129.
949096.
KEGGiecj:Y75_p1582.
eco:b1606.
PATRICi32118514. VBIEscCol129921_1677.

Organism-specific databases

EchoBASEiEB2981.
EcoGeneiEG13189. folM.

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiP0AFS3.
KOiK13938.
OMAiAVIHNAS.
OrthoDBiEOG6WDSG3.
PhylomeDBiP0AFS3.

Enzyme and pathway databases

BioCyciEcoCyc:G6862-MONOMER.
ECOL316407:JW1598-MONOMER.
MetaCyc:G6862-MONOMER.
SABIO-RKP0AFS3.

Miscellaneous databases

PROiP0AFS3.

Gene expression databases

GenevestigatoriP0AFS3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Kuempel P.L.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-240.
    Strain: K12.
  5. "FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli."
    Giladi M., Altman-Price N., Levin I., Levy L., Mevarech M.
    J. Bacteriol. 185:7015-7018(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiFOLM_ECOLI
AccessioniPrimary (citable) accession number: P0AFS3
Secondary accession number(s): P52109, P77386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.