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P0AFS3

- FOLM_ECOLI

UniProt

P0AFS3 - FOLM_ECOLI

Protein

Dihydrofolate reductase FolM

Gene

folM

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of dihydrofolate to tetrahydrofolate.

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.1 Publication

    Enzyme regulationi

    Inhibited by methotrexate.1 Publication

    Kineticsi

    1. KM=9.5 µM for dihydrofolate (at pH 6.0)1 Publication
    2. KM=1.9 µM for NADPH (at pH 6.0)1 Publication

    Vmax=0.083 µmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 4.7.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei152 – 1521Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. dihydromonapterin reductase activity Source: EcoCyc

    GO - Biological processi

    1. 10-formyltetrahydrofolate biosynthetic process Source: EcoCyc
    2. folic acid biosynthetic process Source: EcoCyc
    3. one-carbon metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:G6862-MONOMER.
    ECOL316407:JW1598-MONOMER.
    MetaCyc:G6862-MONOMER.
    SABIO-RKP0AFS3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase FolM (EC:1.5.1.3)
    Short name:
    DHFR
    Gene namesi
    Name:folM
    Synonyms:ydgB
    Ordered Locus Names:b1606, JW1598
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13189. folM.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 240240Dihydrofolate reductase FolMPRO_0000054833Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP0AFS3.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-47981N.
    IntActiP0AFS3. 5 interactions.
    STRINGi511145.b1606.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AFS3.
    SMRiP0AFS3. Positions 8-240.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    KOiK13938.
    OMAiMKIAPGE.
    OrthoDBiEOG6WDSG3.
    PhylomeDBiP0AFS3.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AFS3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA    50
    GAQCIQADFS TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLAD 100
    VLACMMQIHV NTPYLLNHAL ERLLRGHGHA ASDIIHFTDY VVERGSDKHI 150
    AYAASKAALD NMTRSFARKL APEVKVNSIA PSLILFNEHD DAEYRQQALN 200
    KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR 240
    Length:240
    Mass (Da):26,348
    Last modified:December 20, 2005 - v1
    Checksum:i73B942EE144E2CC3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti210 – 2101E → Q1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74678.1.
    AP009048 Genomic DNA. Translation: BAA15344.1.
    U41101 Genomic DNA. No translation available.
    PIRiH64916.
    RefSeqiNP_416123.1. NC_000913.3.
    YP_489869.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74678; AAC74678; b1606.
    BAA15344; BAA15344; BAA15344.
    GeneIDi12933129.
    949096.
    KEGGiecj:Y75_p1582.
    eco:b1606.
    PATRICi32118514. VBIEscCol129921_1677.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74678.1 .
    AP009048 Genomic DNA. Translation: BAA15344.1 .
    U41101 Genomic DNA. No translation available.
    PIRi H64916.
    RefSeqi NP_416123.1. NC_000913.3.
    YP_489869.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AFS3.
    SMRi P0AFS3. Positions 8-240.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47981N.
    IntActi P0AFS3. 5 interactions.
    STRINGi 511145.b1606.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74678 ; AAC74678 ; b1606 .
    BAA15344 ; BAA15344 ; BAA15344 .
    GeneIDi 12933129.
    949096.
    KEGGi ecj:Y75_p1582.
    eco:b1606.
    PATRICi 32118514. VBIEscCol129921_1677.

    Organism-specific databases

    EchoBASEi EB2981.
    EcoGenei EG13189. folM.

    Phylogenomic databases

    eggNOGi COG1028.
    KOi K13938.
    OMAi MKIAPGE.
    OrthoDBi EOG6WDSG3.
    PhylomeDBi P0AFS3.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6862-MONOMER.
    ECOL316407:JW1598-MONOMER.
    MetaCyc:G6862-MONOMER.
    SABIO-RK P0AFS3.

    Miscellaneous databases

    PROi P0AFS3.

    Gene expression databases

    Genevestigatori P0AFS3.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Kuempel P.L.
      Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-240.
      Strain: K12.
    5. "FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli."
      Giladi M., Altman-Price N., Levin I., Levy L., Mevarech M.
      J. Bacteriol. 185:7015-7018(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiFOLM_ECOLI
    AccessioniPrimary (citable) accession number: P0AFS3
    Secondary accession number(s): P52109, P77386
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3