Dihydrofolate reductase FolM - Escherichia coli (strain K12)

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P0AFS3 (FOLM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 67. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Dihydrofolate reductase FolM
Short name=DHFR
EC=1.5.1.3

Gene names

Name:	folM
Synonyms:	ydgB
Ordered Locus Names:	b1606, JW1598

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reduction of dihydrofolate to tetrahydrofolate.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH. Ref.5
Enzyme regulation	Inhibited by methotrexate. Ref.5
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily.
Biophysicochemical properties	Kinetic parameters: K_M=9.5 µM for dihydrofolate (at pH 6.0) Ref.5 K_M=1.9 µM for NADPH (at pH 6.0) V_max=0.083 µmol/min/mg enzyme pH dependence: Optimum pH is 4.7.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	10-formyltetrahydrofolate biosynthetic process Inferred from genetic interaction Ref.5. Source: EcoCyc folic acid biosynthetic process Inferred from genetic interaction Ref.5. Source: EcoCyc one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	dihydrofolate reductase activity Inferred from electronic annotation. Source: EC dihydromonapterin reductase activity Inferred from direct assay PubMed 19897652. Source: EcoCyc nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 240

240

Dihydrofolate reductase FolM

PRO_0000054833

Sites

Active site

152

Proton acceptor By similarity

Experimental info

Sequence conflict

210

E → Q Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P0AFS3 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 73B942EE144E2CC3
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FASTA

240

26,348

        10         20         30         40         50         60 
MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA GAQCIQADFS 

        70         80         90        100        110        120 
TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLAD VLACMMQIHV NTPYLLNHAL 

       130        140        150        160        170        180 
ERLLRGHGHA ASDIIHFTDY VVERGSDKHI AYAASKAALD NMTRSFARKL APEVKVNSIA 

       190        200        210        220        230        240 
PSLILFNEHD DAEYRQQALN KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	Kuempel P.L. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-240. Strain: K12.
[5]	"FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli." Giladi M., Altman-Price N., Levin I., Levy L., Mevarech M. J. Bacteriol. 185:7015-7018(2003) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74678.1. AP009048 Genomic DNA. Translation: BAA15344.1. U41101 Genomic DNA. No translation available.
PIR	H64916.
RefSeq	NP_416123.1. NC_000913.2. YP_489869.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AFS3.
SMR	P0AFS3. Positions 8-240.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47981N.
IntAct	P0AFS3. 5 interactions.
STRING	511145.b1606.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74678; AAC74678; b1606. BAA15344; BAA15344; BAA15344.
GeneID	12933129. 949096.
KEGG	ecj:Y75_p1582. eco:b1606.
PATRIC	32118514. VBIEscCol129921_1677.
Organism-specific databases
EchoBASE	EB2981.
EcoGene	EG13189. folM.
Phylogenomic databases
eggNOG	COG1028.
KO	K13938.
OMA	IHNASDW.
ProtClustDB	PRK06483.
Enzyme and pathway databases
BioCyc	EcoCyc:G6862-MONOMER. ECOL316407:JW1598-MONOMER. MetaCyc:G6862-MONOMER.
Gene expression databases
Genevestigator	P0AFS3.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLM_ECOLI

Accession

Primary (citable) accession number: P0AFS3
Secondary accession number(s): P52109, P77386

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents