Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dihydromonapterin reductase

Gene

folM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of dihydromonapterin to tetrahydromonapterin. Also has lower activity with dihydrofolate.1 Publication

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.1 Publication
5,6,7,8-tetrahydromonapterin + NADP+ = 7,8-dihydromonapterin + NADPH.1 Publication

Enzyme regulationi

Inhibited by methotrexate.1 Publication

Kineticsi

  1. KM=9.5 µM for 7,8-dihydrofolate (at pH 6.0)1 Publication
  2. KM=147 µM for 7,8-dihydromonapterin (at pH 6.0)1 Publication
  3. KM=1.9 µM for NADPH (at pH 6.0)1 Publication
  1. Vmax=0.083 µmol/min/mg enzyme with 7,8-dihydrofolate as substrate1 Publication
  2. Vmax=5.99 µmol/min/mg enzyme with 7,8-dihydromoapterin as substrate1 Publication

pH dependencei

Optimum pH is 4.7.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei152Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • dihydrofolate reductase activity Source: UniProtKB-EC
  • dihydromonapterin reductase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

BioCyciEcoCyc:G6862-MONOMER
MetaCyc:G6862-MONOMER
SABIO-RKP0AFS3

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydromonapterin reductase1 Publication (EC:1.5.1.-1 Publication)
Short name:
H(2)-MPt reductase1 Publication
Alternative name(s):
Dihydrofolate reductase1 Publication (EC:1.5.1.31 Publication)
Short name:
DHFR
Gene namesi
Name:folM1 Publication
Synonyms:ydgB
Ordered Locus Names:b1606, JW1598
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13189 folM

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000548331 – 240Dihydromonapterin reductaseAdd BLAST240

Proteomic databases

PaxDbiP0AFS3
PRIDEiP0AFS3

Interactioni

Protein-protein interaction databases

BioGridi4262123, 119 interactors
DIPiDIP-47981N
IntActiP0AFS3, 5 interactors
STRINGi316385.ECDH10B_1739

Structurei

3D structure databases

ProteinModelPortaliP0AFS3
SMRiP0AFS3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410642E Bacteria
COG1028 LUCA
InParanoidiP0AFS3
KOiK13938
OMAiPALLMFN
PhylomeDBiP0AFS3

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PRINTSiPR00081 GDHRDH
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA
60 70 80 90 100
GAQCIQADFS TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLAD
110 120 130 140 150
VLACMMQIHV NTPYLLNHAL ERLLRGHGHA ASDIIHFTDY VVERGSDKHI
160 170 180 190 200
AYAASKAALD NMTRSFARKL APEVKVNSIA PSLILFNEHD DAEYRQQALN
210 220 230 240
KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR
Length:240
Mass (Da):26,348
Last modified:December 20, 2005 - v1
Checksum:i73B942EE144E2CC3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti210E → Q (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74678.1
AP009048 Genomic DNA Translation: BAA15344.1
U41101 Genomic DNA No translation available.
PIRiH64916
RefSeqiNP_416123.1, NC_000913.3
WP_000520804.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74678; AAC74678; b1606
BAA15344; BAA15344; BAA15344
GeneIDi949096
KEGGiecj:JW1598
eco:b1606
PATRICifig|1411691.4.peg.656

Similar proteinsi

Entry informationi

Entry nameiFOLM_ECOLI
AccessioniPrimary (citable) accession number: P0AFS3
Secondary accession number(s): P52109, P77386
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health