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P0AFS3

- FOLM_ECOLI

UniProt

P0AFS3 - FOLM_ECOLI

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Protein

Dihydrofolate reductase FolM

Gene
folM, ydgB, b1606, JW1598
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of dihydrofolate to tetrahydrofolate.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.1 Publication

Enzyme regulationi

Inhibited by methotrexate.1 Publication

Kineticsi

  1. KM=9.5 µM for dihydrofolate (at pH 6.0)1 Publication
  2. KM=1.9 µM for NADPH (at pH 6.0)

Vmax=0.083 µmol/min/mg enzyme

pH dependencei

Optimum pH is 4.7.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521Proton acceptor By similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. dihydromonapterin reductase activity Source: EcoCyc

GO - Biological processi

  1. 10-formyltetrahydrofolate biosynthetic process Source: EcoCyc
  2. folic acid biosynthetic process Source: EcoCyc
  3. one-carbon metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:G6862-MONOMER.
ECOL316407:JW1598-MONOMER.
MetaCyc:G6862-MONOMER.
SABIO-RKP0AFS3.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase FolM (EC:1.5.1.3)
Short name:
DHFR
Gene namesi
Name:folM
Synonyms:ydgB
Ordered Locus Names:b1606, JW1598
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13189. folM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Dihydrofolate reductase FolMPRO_0000054833Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP0AFS3.

Interactioni

Protein-protein interaction databases

DIPiDIP-47981N.
IntActiP0AFS3. 5 interactions.
STRINGi511145.b1606.

Structurei

3D structure databases

ProteinModelPortaliP0AFS3.
SMRiP0AFS3. Positions 8-240.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK13938.
OMAiMKIAPGE.
OrthoDBiEOG6WDSG3.
PhylomeDBiP0AFS3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFS3-1 [UniParc]FASTAAdd to Basket

« Hide

MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA    50
GAQCIQADFS TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLAD 100
VLACMMQIHV NTPYLLNHAL ERLLRGHGHA ASDIIHFTDY VVERGSDKHI 150
AYAASKAALD NMTRSFARKL APEVKVNSIA PSLILFNEHD DAEYRQQALN 200
KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR 240
Length:240
Mass (Da):26,348
Last modified:December 20, 2005 - v1
Checksum:i73B942EE144E2CC3
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti210 – 2101E → Q1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74678.1.
AP009048 Genomic DNA. Translation: BAA15344.1.
U41101 Genomic DNA. No translation available.
PIRiH64916.
RefSeqiNP_416123.1. NC_000913.3.
YP_489869.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74678; AAC74678; b1606.
BAA15344; BAA15344; BAA15344.
GeneIDi12933129.
949096.
KEGGiecj:Y75_p1582.
eco:b1606.
PATRICi32118514. VBIEscCol129921_1677.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74678.1 .
AP009048 Genomic DNA. Translation: BAA15344.1 .
U41101 Genomic DNA. No translation available.
PIRi H64916.
RefSeqi NP_416123.1. NC_000913.3.
YP_489869.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AFS3.
SMRi P0AFS3. Positions 8-240.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47981N.
IntActi P0AFS3. 5 interactions.
STRINGi 511145.b1606.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74678 ; AAC74678 ; b1606 .
BAA15344 ; BAA15344 ; BAA15344 .
GeneIDi 12933129.
949096.
KEGGi ecj:Y75_p1582.
eco:b1606.
PATRICi 32118514. VBIEscCol129921_1677.

Organism-specific databases

EchoBASEi EB2981.
EcoGenei EG13189. folM.

Phylogenomic databases

eggNOGi COG1028.
KOi K13938.
OMAi MKIAPGE.
OrthoDBi EOG6WDSG3.
PhylomeDBi P0AFS3.

Enzyme and pathway databases

BioCyci EcoCyc:G6862-MONOMER.
ECOL316407:JW1598-MONOMER.
MetaCyc:G6862-MONOMER.
SABIO-RK P0AFS3.

Miscellaneous databases

PROi P0AFS3.

Gene expression databases

Genevestigatori P0AFS3.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Kuempel P.L.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-240.
    Strain: K12.
  5. "FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli."
    Giladi M., Altman-Price N., Levin I., Levy L., Mevarech M.
    J. Bacteriol. 185:7015-7018(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiFOLM_ECOLI
AccessioniPrimary (citable) accession number: P0AFS3
Secondary accession number(s): P52109, P77386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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