Reviewed,
UniProtKB/Swiss-Prot P0AFS3 (FOLM_ECOLI)
Last modified
November 3, 2009.
Version 36.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Dihydrofolate reductase folM Short name=DHFR EC=1.5.1.3 | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 240 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the reduction of dihydrofolate to tetrahydrofolate. |
| Catalytic activity | 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. Ref.5 |
| Enzyme regulation | Inhibited by methotrexate. Ref.5 |
| Sequence similarities | Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily. |
| Biophysicochemical properties | Kinetic parameters: KM=9.5 µM for dihydrofolate (at pH 6.0) KM=1.9 µM for NADPH (at pH 6.0) Vmax=0.083 µmol/min/mg enzyme pH dependence: Optimum pH is 4.7. |
Ontologies
| Keywords | |
|---|---|
| Biological process | One-carbon metabolism |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T.DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [2] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [4] | Kuempel P.L. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-240. Strain: K12. |
| [5] | "FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli." Giladi M., Altman-Price N., Levin I., Levy L., Mevarech M. J. Bacteriol. 185:7015-7018(2003) [PubMed: 14617668] [Abstract] Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: K12 / MG1655 / ATCC 47076. |
Cross-references
Sequence databases | |
|---|---|
| U00096 Genomic DNA. Translation: AAC74678.1. AP009048 Genomic DNA. Translation: BAA15344.1. U41101 Genomic DNA. No translation available. | |
| PIR | H64916. |
| RefSeq | AP_002227.1. NP_416123.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P0AFS3. |
Genome annotation databases | |
| GeneID | 949096. |
| GenomeReviews | Gene locus JW1598 in contig AP009048_GR. Gene locus b1606 in contig U00096_GR. |
| KEGG | ecj:JW1598. eco:b1606. |
Organism-specific databases | |
| EchoBASE | EB2981. |
| EcoGene | EG13189. folM. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P0AFS3. |
| OMA | RAIIHNA. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:G6862-MON. MetaCyc:G6862-MON. |
Gene expression databases | |
| Genevestigator | P0AFS3. |
Family and domain databases | |
| InterPro | IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR19410. ADH_short_C2. 1 hit. |
| Pfam | PF00106. adh_short. 1 hit. [Graphical view] |
| PRINTS | PR00081. GDHRDH. |
| PROSITE | PS00061. ADH_SHORT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | FOLM_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0AFS3 Secondary accession number(s): P52109, P77386 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

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