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P0AFS3 (FOLM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase FolM

Short name=DHFR
EC=1.5.1.3
Gene names
Name:folM
Synonyms:ydgB
Ordered Locus Names:b1606, JW1598
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reduction of dihydrofolate to tetrahydrofolate.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. Ref.5

Enzyme regulation

Inhibited by methotrexate. Ref.5

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=9.5 µM for dihydrofolate (at pH 6.0) Ref.5

KM=1.9 µM for NADPH (at pH 6.0)

Vmax=0.083 µmol/min/mg enzyme

pH dependence:

Optimum pH is 4.7.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240Dihydrofolate reductase FolM
PRO_0000054833

Sites

Active site1521Proton acceptor By similarity

Experimental info

Sequence conflict2101E → Q Ref.4

Sequences

Sequence LengthMass (Da)Tools
P0AFS3 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 73B942EE144E2CC3

FASTA24026,348
        10         20         30         40         50         60 
MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA GAQCIQADFS 

        70         80         90        100        110        120 
TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLAD VLACMMQIHV NTPYLLNHAL 

       130        140        150        160        170        180 
ERLLRGHGHA ASDIIHFTDY VVERGSDKHI AYAASKAALD NMTRSFARKL APEVKVNSIA 

       190        200        210        220        230        240 
PSLILFNEHD DAEYRQQALN KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR 

« Hide

References

« Hide 'large scale' references
[1]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]Kuempel P.L.
Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-240.
Strain: K12.
[5]"FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli."
Giladi M., Altman-Price N., Levin I., Levy L., Mevarech M.
J. Bacteriol. 185:7015-7018(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74678.1.
AP009048 Genomic DNA. Translation: BAA15344.1.
U41101 Genomic DNA. No translation available.
PIRH64916.
RefSeqNP_416123.1. NC_000913.3.
YP_489869.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AFS3.
SMRP0AFS3. Positions 8-240.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47981N.
IntActP0AFS3. 5 interactions.
STRING511145.b1606.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74678; AAC74678; b1606.
BAA15344; BAA15344; BAA15344.
GeneID12933129.
949096.
KEGGecj:Y75_p1582.
eco:b1606.
PATRIC32118514. VBIEscCol129921_1677.

Organism-specific databases

EchoBASEEB2981.
EcoGeneEG13189. folM.

Phylogenomic databases

eggNOGCOG1028.
KOK13938.
OMAMKIAPGE.
OrthoDBEOG6WDSG3.
PhylomeDBP0AFS3.

Enzyme and pathway databases

BioCycEcoCyc:G6862-MONOMER.
ECOL316407:JW1598-MONOMER.
MetaCyc:G6862-MONOMER.
SABIO-RKP0AFS3.

Gene expression databases

GenevestigatorP0AFS3.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0AFS3.

Entry information

Entry nameFOLM_ECOLI
AccessionPrimary (citable) accession number: P0AFS3
Secondary accession number(s): P52109, P77386
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene