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Protein

Dihydromonapterin reductase

Gene

folM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of dihydromonapterin to tetrahydromonapterin. Also has lower activity with dihydrofolate.1 Publication

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.1 Publication
5,6,7,8-tetrahydromonapterin + NADP+ = 7,8-dihydromonapterin + NADPH.1 Publication

Enzyme regulationi

Inhibited by methotrexate.1 Publication

Kineticsi

  1. KM=9.5 µM for 7,8-dihydrofolate (at pH 6.0)1 Publication
  2. KM=147 µM for 7,8-dihydromonapterin (at pH 6.0)1 Publication
  3. KM=1.9 µM for NADPH (at pH 6.0)1 Publication
  1. Vmax=0.083 µmol/min/mg enzyme with 7,8-dihydrofolate as substrate1 Publication
  2. Vmax=5.99 µmol/min/mg enzyme with 7,8-dihydromoapterin as substrate1 Publication

pH dependencei

Optimum pH is 4.7.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei152Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • dihydrofolate reductase activity Source: UniProtKB-EC
  • dihydromonapterin reductase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

BioCyciEcoCyc:G6862-MONOMER.
MetaCyc:G6862-MONOMER.
SABIO-RKiP0AFS3.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydromonapterin reductase1 Publication (EC:1.5.1.-1 Publication)
Short name:
H(2)-MPt reductase1 Publication
Alternative name(s):
Dihydrofolate reductase1 Publication (EC:1.5.1.31 Publication)
Short name:
DHFR
Gene namesi
Name:folM1 Publication
Synonyms:ydgB
Ordered Locus Names:b1606, JW1598
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13189. folM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000548331 – 240Dihydromonapterin reductaseAdd BLAST240

Proteomic databases

PaxDbiP0AFS3.
PRIDEiP0AFS3.

Interactioni

Protein-protein interaction databases

BioGridi4262123. 119 interactors.
DIPiDIP-47981N.
IntActiP0AFS3. 5 interactors.
STRINGi316385.ECDH10B_1739.

Structurei

3D structure databases

ProteinModelPortaliP0AFS3.
SMRiP0AFS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410642E. Bacteria.
COG1028. LUCA.
InParanoidiP0AFS3.
KOiK13938.
PhylomeDBiP0AFS3.

Family and domain databases

InterProiView protein in InterPro
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00061. ADH_SHORT. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA
60 70 80 90 100
GAQCIQADFS TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLAD
110 120 130 140 150
VLACMMQIHV NTPYLLNHAL ERLLRGHGHA ASDIIHFTDY VVERGSDKHI
160 170 180 190 200
AYAASKAALD NMTRSFARKL APEVKVNSIA PSLILFNEHD DAEYRQQALN
210 220 230 240
KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR
Length:240
Mass (Da):26,348
Last modified:December 20, 2005 - v1
Checksum:i73B942EE144E2CC3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti210E → Q (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74678.1.
AP009048 Genomic DNA. Translation: BAA15344.1.
U41101 Genomic DNA. No translation available.
PIRiH64916.
RefSeqiNP_416123.1. NC_000913.3.
WP_000520804.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74678; AAC74678; b1606.
BAA15344; BAA15344; BAA15344.
GeneIDi949096.
KEGGiecj:JW1598.
eco:b1606.
PATRICifig|1411691.4.peg.656.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiFOLM_ECOLI
AccessioniPrimary (citable) accession number: P0AFS3
Secondary accession number(s): P52109, P77386
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: July 5, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families