Reviewed, UniProtKB/Swiss-Prot P0AFS3 (FOLM_ECOLI)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase folM
      Short name=DHFR
    EC=1.5.1.3

Gene names

Name:	folM
Synonyms:	ydgB
Ordered Locus Names:	b1606, JW1598

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reduction of dihydrofolate to tetrahydrofolate.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. Ref.5
Enzyme regulation	Inhibited by methotrexate. Ref.5
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily.
Biophysicochemical properties	Kinetic parameters: KM=9.5 µM for dihydrofolate (at pH 6.0) KM=1.9 µM for NADPH (at pH 6.0) Vmax=0.083 µmol/min/mg enzyme pH dependence: Optimum pH is 4.7.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Dihydrofolate reductase folM		PRO_0000054833
Sites
	Active site	152	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	210	1	E → Q Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P0AFS3-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 73B942EE144E2CC3	FASTA	240	26,348
	10 20 30 40 50 60 MGKTQPLPIL ITGGGRRIGL ALAWHFINQK QPVIVSYRTH YPAIDGLINA GAQCIQADFS 70 80 90 100 110 120 TNDGVMAFAD EVLKSTHGLR AILHNASAWM AEKPGAPLAD VLACMMQIHV NTPYLLNHAL 130 140 150 160 170 180 ERLLRGHGHA ASDIIHFTDY VVERGSDKHI AYAASKAALD NMTRSFARKL APEVKVNSIA 190 200 210 220 230 240 PSLILFNEHD DAEYRQQALN KSLMKTAPGE KEVIDLVDYL LTSCFVTGRS FPLDGGRHLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	Kuempel P.L. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-240. Strain: K12.
[5]	"FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli." Giladi M., Altman-Price N., Levin I., Levy L., Mevarech M. J. Bacteriol. 185:7015-7018(2003) [PubMed: 14617668] [Abstract] Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00096 Genomic DNA. Translation: AAC74678.1. AP009048 Genomic DNA. Translation: BAA15344.1. U41101 Genomic DNA. No translation available.
PIR	H64916.
RefSeq	AP_002227.1. NP_416123.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P0AFS3.
Genome annotation databases
GeneID	949096.
GenomeReviews	Gene locus JW1598 in contig AP009048_GR. Gene locus b1606 in contig U00096_GR.
KEGG	ecj:JW1598. eco:b1606.
Organism-specific databases
EchoBASE	EB2981.
EcoGene	EG13189. folM.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AFS3.
OMA	RAIIHNA.
Enzyme and pathway databases
BioCyc	EcoCyc:G6862-MON. MetaCyc:G6862-MON.
Gene expression databases
Genevestigator	P0AFS3.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLM_ECOLI

Accession

Primary (citable) accession number: P0AFS3
Secondary accession number(s): P52109, P77386

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)