Autoinducer 2 import system permease protein LsrD

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P0AFS1 (LSRD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 61. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Autoinducer 2 import system permease protein LsrD
Short name=AI-2 import system permease protein LsrD

Gene names

Name:	lsrD
Synonyms:	ydeZ
Ordered Locus Names:	b1515, JW1508

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Probably responsible for the translocation of the substrate across the membrane Probable. Ref.4
Subunit structure	The complex is composed of two ATP-binding proteins (LsrA), two transmembrane proteins (LsrC and LsrD) and a solute-binding protein (LsrB) Probable.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Induction	In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter. Ref.4 Ref.5
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. AraH/RbsC subfamily.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 330

330

Autoinducer 2 import system permease protein LsrD

PRO_0000060248

Regions

Topological domain

1 – 4

Cytoplasmic Potential

Transmembrane

5 – 25

Helical; Potential

Topological domain

26 – 42

Periplasmic Potential

Transmembrane

43 – 63

Helical; Potential

Topological domain

64 – 67

Cytoplasmic Potential

Transmembrane

68 – 88

Helical; Potential

Transmembrane

89 – 109

Helical; Potential

Topological domain

110 – 115

Cytoplasmic Potential

Transmembrane

116 – 136

Helical; Potential

Topological domain

137 – 159

Periplasmic Potential

Transmembrane

160 – 180

Helical; Potential

Topological domain

181 – 209

Cytoplasmic Potential

Transmembrane

210 – 230

Helical; Potential

Topological domain

231 – 237

Periplasmic Potential

Transmembrane

238 – 258

Helical; Potential

Transmembrane

259 – 279

Helical; Potential

Topological domain

280 – 285

Periplasmic Potential

Transmembrane

286 – 306

Helical; Potential

Topological domain

307 – 330

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P0AFS1 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 87CDB0958FB81C1E
Show »

FASTA

330

34,456

        10         20         30         40         50         60 
MRIRYGWELA LAALLVIEIV AFGAINPRML DLNMLLFSTS DFICIGIVAL PLTMVIVSGG 

        70         80         90        100        110        120 
IDISFGSTIG LCAIALGVLF QSGVPMPLAI LLTLLLGALC GLINAGLIIY TKVNPLVITL 

       130        140        150        160        170        180 
GTLYLFAGSA LLLSGMAGAT GYEGIGGFPM AFTDFANLDV LGLPVPLIIF LICLLVFWLW 

       190        200        210        220        230        240 
LHKTHAGRNV FLIGQSPRVA LYSAIPVNRT LCALYAMTGL ASAVAAVLLV SYFGSARSDL 

       250        260        270        280        290        300 
GASFLMPAIT AVVLGGANIY GGSGSIIGTA IAVLLVGYLQ QGLQMAGVPN QVSSALSGAL 

       310        320        330 
LIVVVVGRSV SLHRQQIKEW LARRANNPLP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli." Xavier K.B., Bassler B.L. J. Bacteriol. 187:238-248(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN AI-2 IMPORT, INDUCTION. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli." Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E. J. Bacteriol. 187:2066-2076(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74588.1. AP009048 Genomic DNA. Translation: BAA15202.1.
PIR	F64905.
RefSeq	NP_416032.1. NC_000913.2. YP_489778.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AFS1.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1515.
Protein family/group databases
TCDB	3.A.1.2.8. ATP-binding cassette (ABC) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74588; AAC74588; b1515. BAA15202; BAA15202; BAA15202.
GeneID	12930122. 946264.
KEGG	ecj:Y75_p1490. eco:b1515.
PATRIC	32118326. VBIEscCol129921_1583.
Organism-specific databases
EchoBASE	EB3569.
EcoGene	EG13808. lsrD.
Phylogenomic databases
eggNOG	COG1172.
HOGENOM	HOG000212232.
KO	K10557.
OMA	GIMTKKV.
ProtClustDB	PRK15038.
Enzyme and pathway databases
BioCyc	EcoCyc:YDEZ-MONOMER. ECOL316407:JW1508-MONOMER.
Gene expression databases
Genevestigator	P0AFS1.
Family and domain databases
InterPro	IPR001851. ABC_transp_permease. [Graphical view]
Pfam	PF02653. BPD_transp_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSRD_ECOLI

Accession

Primary (citable) accession number: P0AFS1
Secondary accession number(s): P76881, P77651

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents