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Protein

Autoinducer 2 import system permease protein LsrD

Gene

lsrD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Probably responsible for the translocation of the substrate across the membrane (Probable).1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:YDEZ-MONOMER.
ECOL316407:JW1508-MONOMER.
MetaCyc:YDEZ-MONOMER.

Protein family/group databases

TCDBi3.A.1.2.8. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Autoinducer 2 import system permease protein LsrD
Short name:
AI-2 import system permease protein LsrD
Gene namesi
Name:lsrD
Synonyms:ydeZ
Ordered Locus Names:b1515, JW1508
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13808. lsrD.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44CytoplasmicSequence analysis
Transmembranei5 – 2521HelicalSequence analysisAdd
BLAST
Topological domaini26 – 4217PeriplasmicSequence analysisAdd
BLAST
Transmembranei43 – 6321HelicalSequence analysisAdd
BLAST
Topological domaini64 – 674CytoplasmicSequence analysis
Transmembranei68 – 8821HelicalSequence analysisAdd
BLAST
Transmembranei89 – 10921HelicalSequence analysisAdd
BLAST
Topological domaini110 – 1156CytoplasmicSequence analysis
Transmembranei116 – 13621HelicalSequence analysisAdd
BLAST
Topological domaini137 – 15923PeriplasmicSequence analysisAdd
BLAST
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST
Topological domaini181 – 20929CytoplasmicSequence analysisAdd
BLAST
Transmembranei210 – 23021HelicalSequence analysisAdd
BLAST
Topological domaini231 – 2377PeriplasmicSequence analysis
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Transmembranei259 – 27921HelicalSequence analysisAdd
BLAST
Topological domaini280 – 2856PeriplasmicSequence analysis
Transmembranei286 – 30621HelicalSequence analysisAdd
BLAST
Topological domaini307 – 33024CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Autoinducer 2 import system permease protein LsrDPRO_0000060248Add
BLAST

Proteomic databases

PaxDbiP0AFS1.

Expressioni

Inductioni

In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.2 Publications

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (LsrA), two transmembrane proteins (LsrC and LsrD) and a solute-binding protein (LsrB).Curated

Protein-protein interaction databases

BioGridi4259116. 6 interactions.
STRINGi511145.b1515.

Structurei

3D structure databases

ProteinModelPortaliP0AFS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105F5R. Bacteria.
ENOG410XS1A. LUCA.
HOGENOMiHOG000212232.
InParanoidiP0AFS1.
KOiK10557.
OMAiLNPRMLD.
OrthoDBiEOG61P6T8.
PhylomeDBiP0AFS1.

Family and domain databases

InterProiIPR001851. ABC_transp_permease.
[Graphical view]
PfamiPF02653. BPD_transp_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIRYGWELA LAALLVIEIV AFGAINPRML DLNMLLFSTS DFICIGIVAL
60 70 80 90 100
PLTMVIVSGG IDISFGSTIG LCAIALGVLF QSGVPMPLAI LLTLLLGALC
110 120 130 140 150
GLINAGLIIY TKVNPLVITL GTLYLFAGSA LLLSGMAGAT GYEGIGGFPM
160 170 180 190 200
AFTDFANLDV LGLPVPLIIF LICLLVFWLW LHKTHAGRNV FLIGQSPRVA
210 220 230 240 250
LYSAIPVNRT LCALYAMTGL ASAVAAVLLV SYFGSARSDL GASFLMPAIT
260 270 280 290 300
AVVLGGANIY GGSGSIIGTA IAVLLVGYLQ QGLQMAGVPN QVSSALSGAL
310 320 330
LIVVVVGRSV SLHRQQIKEW LARRANNPLP
Length:330
Mass (Da):34,456
Last modified:December 20, 2005 - v1
Checksum:i87CDB0958FB81C1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74588.1.
AP009048 Genomic DNA. Translation: BAA15202.1.
PIRiF64905.
RefSeqiNP_416032.1. NC_000913.3.
WP_001222721.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74588; AAC74588; b1515.
BAA15202; BAA15202; BAA15202.
GeneIDi946264.
KEGGiecj:JW1508.
eco:b1515.
PATRICi32118326. VBIEscCol129921_1583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74588.1.
AP009048 Genomic DNA. Translation: BAA15202.1.
PIRiF64905.
RefSeqiNP_416032.1. NC_000913.3.
WP_001222721.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFS1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259116. 6 interactions.
STRINGi511145.b1515.

Protein family/group databases

TCDBi3.A.1.2.8. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP0AFS1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74588; AAC74588; b1515.
BAA15202; BAA15202; BAA15202.
GeneIDi946264.
KEGGiecj:JW1508.
eco:b1515.
PATRICi32118326. VBIEscCol129921_1583.

Organism-specific databases

EchoBASEiEB3569.
EcoGeneiEG13808. lsrD.

Phylogenomic databases

eggNOGiENOG4105F5R. Bacteria.
ENOG410XS1A. LUCA.
HOGENOMiHOG000212232.
InParanoidiP0AFS1.
KOiK10557.
OMAiLNPRMLD.
OrthoDBiEOG61P6T8.
PhylomeDBiP0AFS1.

Enzyme and pathway databases

BioCyciEcoCyc:YDEZ-MONOMER.
ECOL316407:JW1508-MONOMER.
MetaCyc:YDEZ-MONOMER.

Miscellaneous databases

PROiP0AFS1.

Family and domain databases

InterProiIPR001851. ABC_transp_permease.
[Graphical view]
PfamiPF02653. BPD_transp_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli."
    Xavier K.B., Bassler B.L.
    J. Bacteriol. 187:238-248(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN AI-2 IMPORT, INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli."
    Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E.
    J. Bacteriol. 187:2066-2076(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiLSRD_ECOLI
AccessioniPrimary (citable) accession number: P0AFS1
Secondary accession number(s): P76881, P77651
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: July 6, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.