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Protein

C4-dicarboxylic acid transporter DauA

Gene

dauA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the aerobic transport of succinate from the periplasm to the cytoplasm at acidic pH. Can transport other C4-dicarboxylic acids such as aspartate and fumarate. May also play a role in the regulation of C4-dicarboxylic acid metabolism at pH 7, via regulation of expression and/or activity of DctA. May act as a co-sensor of DcuS.1 Publication

GO - Molecular functioni

GO - Biological processi

  • aspartate transmembrane transport Source: EcoCyc
  • fumarate transport Source: EcoCyc
  • succinate transmembrane transport Source: EcoCyc

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:YCHM-MONOMER
MetaCyc:YCHM-MONOMER

Protein family/group databases

TCDBi2.A.53.3.11 the sulfate permease (sulp) family

Names & Taxonomyi

Protein namesi
Recommended name:
C4-dicarboxylic acid transporter DauA
Alternative name(s):
Dicarboxylic acid uptake system A
Gene namesi
Name:dauA
Synonyms:ychM
Ordered Locus Names:b1206, JW5189
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12392 dauA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicSequence analysisAdd BLAST32
Transmembranei33 – 53HelicalSequence analysisAdd BLAST21
Topological domaini54PeriplasmicSequence analysis1
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 79CytoplasmicSequence analysis4
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Topological domaini101PeriplasmicSequence analysis1
Transmembranei102 – 122HelicalSequence analysisAdd BLAST21
Topological domaini123 – 126CytoplasmicSequence analysis4
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Topological domaini148 – 177PeriplasmicSequence analysisAdd BLAST30
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 212CytoplasmicSequence analysisAdd BLAST14
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 278PeriplasmicSequence analysisAdd BLAST45
Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
Topological domaini300 – 313CytoplasmicSequence analysisAdd BLAST14
Transmembranei314 – 334HelicalSequence analysisAdd BLAST21
Topological domaini335 – 350PeriplasmicSequence analysisAdd BLAST16
Transmembranei351 – 371HelicalSequence analysisAdd BLAST21
Topological domaini372CytoplasmicSequence analysis1
Transmembranei373 – 393HelicalSequence analysisAdd BLAST21
Topological domaini394 – 418PeriplasmicSequence analysisAdd BLAST25
Transmembranei419 – 439HelicalSequence analysisAdd BLAST21
Topological domaini440 – 559CytoplasmicSequence analysisAdd BLAST120

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant is metabolically inactive when succinate is used as sole carbon source. Does not affect growth on glucose, fructose, maltose and glycerol.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000801951 – 559C4-dicarboxylic acid transporter DauAAdd BLAST559

Proteomic databases

PaxDbiP0AFR2
PRIDEiP0AFR2

Interactioni

Protein-protein interaction databases

BioGridi4259558, 713 interactors
DIPiDIP-48160N
STRINGi316385.ECDH10B_1259

Structurei

Secondary structure

1559
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi446 – 450Combined sources5
Beta strandi459 – 466Combined sources8
Helixi470 – 481Combined sources12
Beta strandi488 – 496Combined sources9
Helixi502 – 514Combined sources13
Beta strandi520 – 524Combined sources5
Helixi528 – 536Combined sources9
Turni543 – 545Combined sources3
Beta strandi546 – 551Combined sources6
Helixi552 – 555Combined sources4
Turni556 – 558Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NY7X-ray1.92A445-559[»]
ProteinModelPortaliP0AFR2
SMRiP0AFR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini449 – 559STASPROSITE-ProRule annotationAdd BLAST111

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CHH Bacteria
COG0659 LUCA
HOGENOMiHOG000236211
InParanoidiP0AFR2
KOiK03321
OMAiTRPRIQI
PhylomeDBiP0AFR2

Family and domain databases

Gene3Di3.30.750.24, 1 hit
InterProiView protein in InterPro
IPR018045 S04_transporter_CS
IPR011547 SLC26A/SulP_dom
IPR001902 SLC26A/SulP_fam
IPR002645 STAS_dom
IPR036513 STAS_dom_sf
PANTHERiPTHR11814 PTHR11814, 1 hit
PfamiView protein in Pfam
PF01740 STAS, 1 hit
PF00916 Sulfate_transp, 1 hit
SUPFAMiSSF52091 SSF52091, 1 hit
TIGRFAMsiTIGR00815 sulP, 1 hit
PROSITEiView protein in PROSITE
PS01130 SLC26A, 1 hit
PS50801 STAS, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIFSSHVM PFRALIDACW KEKYTAARFT RDLIAGITVG IIAIPLAMAL
60 70 80 90 100
AIGSGVAPQY GLYTAAVAGI VIALTGGSRF SVSGPTAAFV VILYPVSQQF
110 120 130 140 150
GLAGLLVATL LSGIFLILMG LARFGRLIEY IPVSVTLGFT SGIGITIGTM
160 170 180 190 200
QIKDFLGLQM AHVPEHYLQK VGALFMALPT INVGDAAIGI VTLGILVFWP
210 220 230 240 250
RLGIRLPGHL PALLAGCAVM GIVNLLGGHV ATIGSQFHYV LADGSQGNGI
260 270 280 290 300
PQLLPQLVLP WDLPNSEFTL TWDSIRTLLP AAFSMAMLGA IESLLCAVVL
310 320 330 340 350
DGMTGTKHKA NSELVGQGLG NIIAPFFGGI TATAAIARSA ANVRAGATSP
360 370 380 390 400
ISAVIHSILV ILALLVLAPL LSWLPLSAMA ALLLMVAWNM SEAHKVVDLL
410 420 430 440 450
RHAPKDDIIV MLLCMSLTVL FDMVIAISVG IVLASLLFMR RIARMTRLAP
460 470 480 490 500
VVVDVPDDVL VLRVIGPLFF AAAEGLFTDL ESRLEGKRIV ILKWDAVPVL
510 520 530 540 550
DAGGLDAFQR FVKRLPEGCE LRVCNVEFQP LRTMARAGIQ PIPGRLAFFP

NRRAAMADL
Length:559
Mass (Da):59,429
Last modified:December 4, 2007 - v2
Checksum:i14EC83860002F23C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111 – 114LSGI → AVGD in X53983 (Ref. 4) Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74290.2
AP009048 Genomic DNA Translation: BAA36064.2
X53983 Genomic DNA No translation available.
M13174 Genomic DNA No translation available.
PIRiC64867
RefSeqiNP_415724.2, NC_000913.3
WP_001033352.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74290; AAC74290; b1206
BAA36064; BAA36064; BAA36064
GeneIDi945770
KEGGiecj:JW5189
eco:b1206
PATRICifig|511145.12.peg.1254

Similar proteinsi

Entry informationi

Entry nameiDAUA_ECOLI
AccessioniPrimary (citable) accession number: P0AFR2
Secondary accession number(s): P40877, P76019
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 4, 2007
Last modified: April 25, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health