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Protein

C4-dicarboxylic acid transporter DauA

Gene

dauA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the aerobic transport of succinate from the periplasm to the cytoplasm at acidic pH. Can transport other C4-dicarboxylic acids such as aspartate and fumarate. May also play a role in the regulation of C4-dicarboxylic acid metabolism at pH 7, via regulation of expression and/or activity of DctA. May act as a co-sensor of DcuS.1 Publication

GO - Molecular functioni

GO - Biological processi

  • aspartate transport Source: EcoCyc
  • fumarate transport Source: EcoCyc
  • L-aspartate transmembrane transport Source: GOC
  • succinate transmembrane transport Source: EcoCyc
  • sulfate transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:YCHM-MONOMER.
ECOL316407:JW5189-MONOMER.
MetaCyc:YCHM-MONOMER.

Protein family/group databases

TCDBi2.A.53.3.11. the sulfate permease (sulp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
C4-dicarboxylic acid transporter DauA
Alternative name(s):
Dicarboxylic acid uptake system A
Gene namesi
Name:dauA
Synonyms:ychM
Ordered Locus Names:b1206, JW5189
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12392. dauA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232CytoplasmicSequence analysisAdd
BLAST
Transmembranei33 – 5321HelicalSequence analysisAdd
BLAST
Topological domaini54 – 541PeriplasmicSequence analysis
Transmembranei55 – 7521HelicalSequence analysisAdd
BLAST
Topological domaini76 – 794CytoplasmicSequence analysis
Transmembranei80 – 10021HelicalSequence analysisAdd
BLAST
Topological domaini101 – 1011PeriplasmicSequence analysis
Transmembranei102 – 12221HelicalSequence analysisAdd
BLAST
Topological domaini123 – 1264CytoplasmicSequence analysis
Transmembranei127 – 14721HelicalSequence analysisAdd
BLAST
Topological domaini148 – 17730PeriplasmicSequence analysisAdd
BLAST
Transmembranei178 – 19821HelicalSequence analysisAdd
BLAST
Topological domaini199 – 21214CytoplasmicSequence analysisAdd
BLAST
Transmembranei213 – 23321HelicalSequence analysisAdd
BLAST
Topological domaini234 – 27845PeriplasmicSequence analysisAdd
BLAST
Transmembranei279 – 29921HelicalSequence analysisAdd
BLAST
Topological domaini300 – 31314CytoplasmicSequence analysisAdd
BLAST
Transmembranei314 – 33421HelicalSequence analysisAdd
BLAST
Topological domaini335 – 35016PeriplasmicSequence analysisAdd
BLAST
Transmembranei351 – 37121HelicalSequence analysisAdd
BLAST
Topological domaini372 – 3721CytoplasmicSequence analysis
Transmembranei373 – 39321HelicalSequence analysisAdd
BLAST
Topological domaini394 – 41825PeriplasmicSequence analysisAdd
BLAST
Transmembranei419 – 43921HelicalSequence analysisAdd
BLAST
Topological domaini440 – 559120CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant is metabolically inactive when succinate is used as sole carbon source. Does not affect growth on glucose, fructose, maltose and glycerol.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 559559C4-dicarboxylic acid transporter DauAPRO_0000080195Add
BLAST

Proteomic databases

PaxDbiP0AFR2.

Interactioni

Protein-protein interaction databases

BioGridi4259558. 626 interactions.
DIPiDIP-48160N.
STRINGi511145.b1206.

Structurei

Secondary structure

1
559
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi446 – 4505Combined sources
Beta strandi459 – 4668Combined sources
Helixi470 – 48112Combined sources
Beta strandi488 – 4969Combined sources
Helixi502 – 51413Combined sources
Beta strandi520 – 5245Combined sources
Helixi528 – 5369Combined sources
Turni543 – 5453Combined sources
Beta strandi546 – 5516Combined sources
Helixi552 – 5554Combined sources
Turni556 – 5583Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NY7X-ray1.92A445-559[»]
ProteinModelPortaliP0AFR2.
SMRiP0AFR2. Positions 31-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini449 – 559111STASPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 STAS domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CHH. Bacteria.
COG0659. LUCA.
HOGENOMiHOG000236211.
InParanoidiP0AFR2.
KOiK03321.
OMAiGMEADNP.
OrthoDBiEOG618QS9.
PhylomeDBiP0AFR2.

Family and domain databases

Gene3Di3.30.750.24. 1 hit.
InterProiIPR018045. S04_transporter_CS.
IPR011547. SLC26A/SulP_dom.
IPR001902. SLC26A/SulP_fam.
IPR002645. STAS_dom.
[Graphical view]
PANTHERiPTHR11814. PTHR11814. 1 hit.
PfamiPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 1 hit.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIFSSHVM PFRALIDACW KEKYTAARFT RDLIAGITVG IIAIPLAMAL
60 70 80 90 100
AIGSGVAPQY GLYTAAVAGI VIALTGGSRF SVSGPTAAFV VILYPVSQQF
110 120 130 140 150
GLAGLLVATL LSGIFLILMG LARFGRLIEY IPVSVTLGFT SGIGITIGTM
160 170 180 190 200
QIKDFLGLQM AHVPEHYLQK VGALFMALPT INVGDAAIGI VTLGILVFWP
210 220 230 240 250
RLGIRLPGHL PALLAGCAVM GIVNLLGGHV ATIGSQFHYV LADGSQGNGI
260 270 280 290 300
PQLLPQLVLP WDLPNSEFTL TWDSIRTLLP AAFSMAMLGA IESLLCAVVL
310 320 330 340 350
DGMTGTKHKA NSELVGQGLG NIIAPFFGGI TATAAIARSA ANVRAGATSP
360 370 380 390 400
ISAVIHSILV ILALLVLAPL LSWLPLSAMA ALLLMVAWNM SEAHKVVDLL
410 420 430 440 450
RHAPKDDIIV MLLCMSLTVL FDMVIAISVG IVLASLLFMR RIARMTRLAP
460 470 480 490 500
VVVDVPDDVL VLRVIGPLFF AAAEGLFTDL ESRLEGKRIV ILKWDAVPVL
510 520 530 540 550
DAGGLDAFQR FVKRLPEGCE LRVCNVEFQP LRTMARAGIQ PIPGRLAFFP

NRRAAMADL
Length:559
Mass (Da):59,429
Last modified:December 4, 2007 - v2
Checksum:i14EC83860002F23C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1144LSGI → AVGD in X53983 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74290.2.
AP009048 Genomic DNA. Translation: BAA36064.2.
X53983 Genomic DNA. No translation available.
M13174 Genomic DNA. No translation available.
PIRiC64867.
RefSeqiNP_415724.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74290; AAC74290; b1206.
BAA36064; BAA36064; BAA36064.
GeneIDi945770.
KEGGiecj:JW5189.
eco:b1206.
PATRICi32117664. VBIEscCol129921_1254.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74290.2.
AP009048 Genomic DNA. Translation: BAA36064.2.
X53983 Genomic DNA. No translation available.
M13174 Genomic DNA. No translation available.
PIRiC64867.
RefSeqiNP_415724.2. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NY7X-ray1.92A445-559[»]
ProteinModelPortaliP0AFR2.
SMRiP0AFR2. Positions 31-559.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259558. 626 interactions.
DIPiDIP-48160N.
STRINGi511145.b1206.

Protein family/group databases

TCDBi2.A.53.3.11. the sulfate permease (sulp) family.

Proteomic databases

PaxDbiP0AFR2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74290; AAC74290; b1206.
BAA36064; BAA36064; BAA36064.
GeneIDi945770.
KEGGiecj:JW5189.
eco:b1206.
PATRICi32117664. VBIEscCol129921_1254.

Organism-specific databases

EchoBASEiEB2293.
EcoGeneiEG12392. dauA.

Phylogenomic databases

eggNOGiENOG4105CHH. Bacteria.
COG0659. LUCA.
HOGENOMiHOG000236211.
InParanoidiP0AFR2.
KOiK03321.
OMAiGMEADNP.
OrthoDBiEOG618QS9.
PhylomeDBiP0AFR2.

Enzyme and pathway databases

BioCyciEcoCyc:YCHM-MONOMER.
ECOL316407:JW5189-MONOMER.
MetaCyc:YCHM-MONOMER.

Miscellaneous databases

PROiP0AFR2.

Family and domain databases

Gene3Di3.30.750.24. 1 hit.
InterProiIPR018045. S04_transporter_CS.
IPR011547. SLC26A/SulP_dom.
IPR001902. SLC26A/SulP_fam.
IPR002645. STAS_dom.
[Graphical view]
PANTHERiPTHR11814. PTHR11814. 1 hit.
PfamiPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 1 hit.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Lloyd R.G.
    Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-114.
    Strain: K12.
  5. "Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene."
    Hove-Jensen B., Harlow K.W., King C.J., Switzer R.L.
    J. Biol. Chem. 261:6765-6771(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 10-66.
  6. "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
    Borodovsky M., Rudd K.E., Koonin E.V.
    Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  7. "Similarities between a soybean nodulin, Neurospora crassa sulphate permease II and a putative human tumour suppressor."
    Sandal N.N., Marcker K.A.
    Trends Biochem. Sci. 19:19-19(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, SIMILARITY TO SULFATE PERMEASES.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  9. "The Escherichia coli SLC26 homologue YchM (DauA) is a C(4) -dicarboxylic acid transporter."
    Karinou E., Compton E.L., Morel M., Javelle A.
    Mol. Microbiol. 87:623-640(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, GENE NAME.
    Strain: K12 / BW25113.

Entry informationi

Entry nameiDAUA_ECOLI
AccessioniPrimary (citable) accession number: P0AFR2
Secondary accession number(s): P40877, P76019
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 4, 2007
Last modified: February 17, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.