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Protein

Uncharacterized metal-dependent hydrolase YcfH

Gene

ycfH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has D-tyrosyl-tRNA deacylase activity in vitro.1 Publication

Cofactori

Co2+1 Publication, Mn2+1 Publication, Ni2+1 PublicationNote: Binds 2 divalent metal cations per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Divalent metal cation 11 Publication1
Metal bindingi9Divalent metal cation 11 Publication1
Metal bindingi94Divalent metal cation 11 Publication1
Metal bindingi94Divalent metal cation 21 Publication1
Metal bindingi130Divalent metal cation 21 Publication1
Metal bindingi155Divalent metal cation 21 Publication1
Metal bindingi205Divalent metal cation 11 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12303-MONOMER.
ECOL316407:JW1086-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized metal-dependent hydrolase YcfHCurated (EC:3.1.-.-Curated)
Gene namesi
Name:ycfH
Ordered Locus Names:b1100, JW1086
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12303. ycfH.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002019951 – 265Uncharacterized metal-dependent hydrolase YcfHAdd BLAST265

Proteomic databases

PaxDbiP0AFQ7.
PRIDEiP0AFQ7.

Interactioni

Protein-protein interaction databases

BioGridi4260943. 192 interactors.
DIPiDIP-48157N.
IntActiP0AFQ7. 8 interactors.
MINTiMINT-1267935.
STRINGi511145.b1100.

Structurei

Secondary structure

1265
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi10 – 12Combined sources3
Turni15 – 18Combined sources4
Helixi22 – 31Combined sources10
Beta strandi34 – 39Combined sources6
Helixi44 – 54Combined sources11
Beta strandi60 – 64Combined sources5
Helixi77 – 84Combined sources8
Beta strandi89 – 98Combined sources10
Helixi106 – 123Combined sources18
Beta strandi127 – 133Combined sources7
Helixi135 – 144Combined sources10
Helixi147 – 149Combined sources3
Beta strandi152 – 154Combined sources3
Helixi161 – 168Combined sources8
Turni169 – 171Combined sources3
Beta strandi173 – 176Combined sources4
Helixi178 – 181Combined sources4
Helixi186 – 194Combined sources9
Helixi197 – 199Combined sources3
Beta strandi200 – 202Combined sources3
Helixi221 – 223Combined sources3
Helixi224 – 235Combined sources12
Helixi239 – 253Combined sources15
Helixi258 – 261Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YIXX-ray1.90A/B1-265[»]
ProteinModelPortaliP0AFQ7.
SMRiP0AFQ7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFQ7.

Family & Domainsi

Sequence similaritiesi

Belongs to the TatD-type hydrolase family.Curated

Phylogenomic databases

eggNOGiENOG4105F8V. Bacteria.
COG0084. LUCA.
HOGENOMiHOG000201520.
InParanoidiP0AFQ7.
KOiK03424.
OMAiFVDHIKV.
PhylomeDBiP0AFQ7.

Family and domain databases

InterProiIPR018228. DNase_TatD-rel_CS.
IPR015991. Hydrolase_TatD-type.
IPR032466. Metal_Hydrolase.
IPR001130. TatD_family.
[Graphical view]
PANTHERiPTHR10060. PTHR10060. 1 hit.
PfamiPF01026. TatD_DNase. 1 hit.
[Graphical view]
PIRSFiPIRSF005902. DNase_TatD. 1 hit.
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR00010. TIGR00010. 1 hit.
PROSITEiPS01137. TATD_1. 1 hit.
PS01091. TATD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLVDSHCHL DGLDYESLHK DVDDVLAKAA ARDVKFCLAV ATTLPGYLHM
60 70 80 90 100
RDLVGERDNV VFSCGVHPLN QNDPYDVEDL RRLAAEEGVV ALGETGLDYY
110 120 130 140 150
YTPETKVRQQ ESFIHHIQIG RELNKPVIVH TRDARADTLA ILREEKVTDC
160 170 180 190 200
GGVLHCFTED RETAGKLLDL GFYISFSGIV TFRNAEQLRD AARYVPLDRL
210 220 230 240 250
LVETDSPYLA PVPHRGKENQ PAMVRDVAEY MAVLKGVAVE ELAQVTTDNF
260
ARLFHIDASR LQSIR
Length:265
Mass (Da):29,809
Last modified:December 20, 2005 - v1
Checksum:i722C9450157620C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97L → Q (PubMed:8509334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74184.1.
AP009048 Genomic DNA. Translation: BAA35907.1.
L01483 Genomic DNA. No translation available.
L04577 Genomic DNA. No translation available.
PIRiA64854.
RefSeqiNP_415618.1. NC_000913.3.
WP_000480245.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74184; AAC74184; b1100.
BAA35907; BAA35907; BAA35907.
GeneIDi945656.
KEGGiecj:JW1086.
eco:b1100.
PATRICi32117441. VBIEscCol129921_1143.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74184.1.
AP009048 Genomic DNA. Translation: BAA35907.1.
L01483 Genomic DNA. No translation available.
L04577 Genomic DNA. No translation available.
PIRiA64854.
RefSeqiNP_415618.1. NC_000913.3.
WP_000480245.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YIXX-ray1.90A/B1-265[»]
ProteinModelPortaliP0AFQ7.
SMRiP0AFQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260943. 192 interactors.
DIPiDIP-48157N.
IntActiP0AFQ7. 8 interactors.
MINTiMINT-1267935.
STRINGi511145.b1100.

Proteomic databases

PaxDbiP0AFQ7.
PRIDEiP0AFQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74184; AAC74184; b1100.
BAA35907; BAA35907; BAA35907.
GeneIDi945656.
KEGGiecj:JW1086.
eco:b1100.
PATRICi32117441. VBIEscCol129921_1143.

Organism-specific databases

EchoBASEiEB2209.
EcoGeneiEG12303. ycfH.

Phylogenomic databases

eggNOGiENOG4105F8V. Bacteria.
COG0084. LUCA.
HOGENOMiHOG000201520.
InParanoidiP0AFQ7.
KOiK03424.
OMAiFVDHIKV.
PhylomeDBiP0AFQ7.

Enzyme and pathway databases

BioCyciEcoCyc:EG12303-MONOMER.
ECOL316407:JW1086-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFQ7.
PROiP0AFQ7.

Family and domain databases

InterProiIPR018228. DNase_TatD-rel_CS.
IPR015991. Hydrolase_TatD-type.
IPR032466. Metal_Hydrolase.
IPR001130. TatD_family.
[Graphical view]
PANTHERiPTHR10060. PTHR10060. 1 hit.
PfamiPF01026. TatD_DNase. 1 hit.
[Graphical view]
PIRSFiPIRSF005902. DNase_TatD. 1 hit.
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR00010. TIGR00010. 1 hit.
PROSITEiPS01137. TATD_1. 1 hit.
PS01091. TATD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYCFH_ECOLI
AccessioniPrimary (citable) accession number: P0AFQ7
Secondary accession number(s): P37346, P78057
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.