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Protein

Uncharacterized metal-dependent hydrolase YcfH

Gene

ycfH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has D-tyrosyl-tRNA deacylase activity in vitro.1 Publication

Cofactori

Co2+1 Publication, Mn2+1 Publication, Ni2+1 PublicationNote: Binds 2 divalent metal cations per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Divalent metal cation 11 Publication1
Metal bindingi9Divalent metal cation 11 Publication1
Metal bindingi94Divalent metal cation 11 Publication1
Metal bindingi94Divalent metal cation 21 Publication1
Metal bindingi130Divalent metal cation 21 Publication1
Metal bindingi155Divalent metal cation 21 Publication1
Metal bindingi205Divalent metal cation 11 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12303-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized metal-dependent hydrolase YcfHCurated (EC:3.1.-.-Curated)
Gene namesi
Name:ycfH
Ordered Locus Names:b1100, JW1086
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12303 ycfH

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002019951 – 265Uncharacterized metal-dependent hydrolase YcfHAdd BLAST265

Proteomic databases

PaxDbiP0AFQ7
PRIDEiP0AFQ7

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
gcdP158772EBI-560527,EBI-545666

Protein-protein interaction databases

BioGridi4260943, 192 interactors
DIPiDIP-48157N
IntActiP0AFQ7, 9 interactors
STRINGi316385.ECDH10B_1172

Structurei

Secondary structure

1265
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi10 – 12Combined sources3
Turni15 – 18Combined sources4
Helixi22 – 31Combined sources10
Beta strandi34 – 39Combined sources6
Helixi44 – 54Combined sources11
Beta strandi60 – 64Combined sources5
Helixi77 – 84Combined sources8
Beta strandi89 – 98Combined sources10
Helixi106 – 123Combined sources18
Beta strandi127 – 133Combined sources7
Helixi135 – 144Combined sources10
Helixi147 – 149Combined sources3
Beta strandi152 – 154Combined sources3
Helixi161 – 168Combined sources8
Turni169 – 171Combined sources3
Beta strandi173 – 176Combined sources4
Helixi178 – 181Combined sources4
Helixi186 – 194Combined sources9
Helixi197 – 199Combined sources3
Beta strandi200 – 202Combined sources3
Helixi221 – 223Combined sources3
Helixi224 – 235Combined sources12
Helixi239 – 253Combined sources15
Helixi258 – 261Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YIXX-ray1.90A/B1-265[»]
ProteinModelPortaliP0AFQ7
SMRiP0AFQ7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFQ7

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F8V Bacteria
COG0084 LUCA
HOGENOMiHOG000201520
InParanoidiP0AFQ7
KOiK03424
OMAiESDGPYE
PhylomeDBiP0AFQ7

Family and domain databases

CDDicd01310 TatD_DNAse, 1 hit
InterProiView protein in InterPro
IPR018228 DNase_TatD-rel_CS
IPR015991 Hydrolase_TatD-type
IPR032466 Metal_Hydrolase
IPR001130 TatD_family
PANTHERiPTHR10060 PTHR10060, 1 hit
PfamiView protein in Pfam
PF01026 TatD_DNase, 1 hit
PIRSFiPIRSF005902 DNase_TatD, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR00010 TIGR00010, 1 hit
PROSITEiView protein in PROSITE
PS01137 TATD_1, 1 hit
PS01091 TATD_3, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLVDSHCHL DGLDYESLHK DVDDVLAKAA ARDVKFCLAV ATTLPGYLHM
60 70 80 90 100
RDLVGERDNV VFSCGVHPLN QNDPYDVEDL RRLAAEEGVV ALGETGLDYY
110 120 130 140 150
YTPETKVRQQ ESFIHHIQIG RELNKPVIVH TRDARADTLA ILREEKVTDC
160 170 180 190 200
GGVLHCFTED RETAGKLLDL GFYISFSGIV TFRNAEQLRD AARYVPLDRL
210 220 230 240 250
LVETDSPYLA PVPHRGKENQ PAMVRDVAEY MAVLKGVAVE ELAQVTTDNF
260
ARLFHIDASR LQSIR
Length:265
Mass (Da):29,809
Last modified:December 20, 2005 - v1
Checksum:i722C9450157620C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97L → Q (PubMed:8509334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74184.1
AP009048 Genomic DNA Translation: BAA35907.1
L01483 Genomic DNA No translation available.
L04577 Genomic DNA No translation available.
PIRiA64854
RefSeqiNP_415618.1, NC_000913.3
WP_000480245.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74184; AAC74184; b1100
BAA35907; BAA35907; BAA35907
GeneIDi945656
KEGGiecj:JW1086
eco:b1100
PATRICifig|1411691.4.peg.1168

Similar proteinsi

Entry informationi

Entry nameiYCFH_ECOLI
AccessioniPrimary (citable) accession number: P0AFQ7
Secondary accession number(s): P37346, P78057
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health