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Protein

Putative aminoacrylate peracid reductase RutC

Gene

rutC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce in vivo, but not in vitro in the pyrimidine nitrogen degradation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei106By similarity1

GO - Molecular functioni

GO - Biological processi

  • nitrogen utilization Source: UniProtKB
  • pyrimidine nucleobase catabolic process Source: EcoCyc
  • uracil catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciEcoCyc:G6521-MONOMER.
ECOL316407:JW0995-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative aminoacrylate peracid reductase RutC (EC:1.-.-.-)
Gene namesi
Name:rutC
Synonyms:ycdK
Ordered Locus Names:b1010, JW0995
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13857. rutC.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to grow on uridine as the sole source of nitrogen at room temperature indicating a probably accumulation of a toxic intermediate, the peracid aminoacrylate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001703191 – 128Putative aminoacrylate peracid reductase RutCAdd BLAST128

Proteomic databases

PaxDbiP0AFQ5.
PRIDEiP0AFQ5.

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.2 Publications

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

BioGridi4260036. 13 interactors.
IntActiP0AFQ5. 7 interactors.
STRINGi511145.b1010.

Structurei

3D structure databases

ProteinModelPortaliP0AFQ5.
SMRiP0AFQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RutC family.Curated

Phylogenomic databases

eggNOGiENOG4107YYT. Bacteria.
COG0251. LUCA.
HOGENOMiHOG000267215.
InParanoidiP0AFQ5.
KOiK09021.
OMAiTIITPPG.
PhylomeDBiP0AFQ5.

Family and domain databases

Gene3Di3.30.1330.40. 1 hit.
HAMAPiMF_00831. RutC. 1 hit.
InterProiIPR013813. Endoribo_LPSP/chorism_mut-like.
IPR019897. RidA_CS.
IPR019898. RutC.
IPR006175. YjgF/YER057c/UK114.
[Graphical view]
PANTHERiPTHR11803. PTHR11803. 1 hit.
PfamiPF01042. Ribonuc_L-PSP. 1 hit.
[Graphical view]
SUPFAMiSSF55298. SSF55298. 1 hit.
TIGRFAMsiTIGR03610. RutC. 1 hit.
PROSITEiPS01094. UPF0076. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKSVIIPAG SSAPLAPFVP GTLADGVVYV SGTLAFDQHN NVLFADDPKA
60 70 80 90 100
QTRHVLETIR KVIETAGGTM ADVTFNSIFI TDWKNYAAIN EIYAEFFPGD
110 120
KPARFCIQCG LVKPDALVEI ATIAHIAK
Length:128
Mass (Da):13,763
Last modified:December 20, 2005 - v1
Checksum:i7D5FC2374597D907
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74095.1.
AP009048 Genomic DNA. Translation: BAA35777.1.
PIRiH64842.
RefSeqiNP_415530.1. NC_000913.3.
WP_001126780.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74095; AAC74095; b1010.
BAA35777; BAA35777; BAA35777.
GeneIDi945599.
KEGGiecj:JW0995.
eco:b1010.
PATRICi32117249. VBIEscCol129921_1048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74095.1.
AP009048 Genomic DNA. Translation: BAA35777.1.
PIRiH64842.
RefSeqiNP_415530.1. NC_000913.3.
WP_001126780.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFQ5.
SMRiP0AFQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260036. 13 interactors.
IntActiP0AFQ5. 7 interactors.
STRINGi511145.b1010.

Proteomic databases

PaxDbiP0AFQ5.
PRIDEiP0AFQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74095; AAC74095; b1010.
BAA35777; BAA35777; BAA35777.
GeneIDi945599.
KEGGiecj:JW0995.
eco:b1010.
PATRICi32117249. VBIEscCol129921_1048.

Organism-specific databases

EchoBASEiEB3617.
EcoGeneiEG13857. rutC.

Phylogenomic databases

eggNOGiENOG4107YYT. Bacteria.
COG0251. LUCA.
HOGENOMiHOG000267215.
InParanoidiP0AFQ5.
KOiK09021.
OMAiTIITPPG.
PhylomeDBiP0AFQ5.

Enzyme and pathway databases

BioCyciEcoCyc:G6521-MONOMER.
ECOL316407:JW0995-MONOMER.

Miscellaneous databases

PROiP0AFQ5.

Family and domain databases

Gene3Di3.30.1330.40. 1 hit.
HAMAPiMF_00831. RutC. 1 hit.
InterProiIPR013813. Endoribo_LPSP/chorism_mut-like.
IPR019897. RidA_CS.
IPR019898. RutC.
IPR006175. YjgF/YER057c/UK114.
[Graphical view]
PANTHERiPTHR11803. PTHR11803. 1 hit.
PfamiPF01042. Ribonuc_L-PSP. 1 hit.
[Graphical view]
SUPFAMiSSF55298. SSF55298. 1 hit.
TIGRFAMsiTIGR03610. RutC. 1 hit.
PROSITEiPS01094. UPF0076. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRUTC_ECOLI
AccessioniPrimary (citable) accession number: P0AFQ5
Secondary accession number(s): P75896
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37 degrees Celsius, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature (RutE/YdfG function).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.