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Protein

Putative aminoacrylate peracid reductase RutC

Gene

rutC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce in vivo, but not in vitro in the pyrimidine nitrogen degradation.1 Publication

Miscellaneous

The Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37 degrees Celsius, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature (RutE/YdfG function).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei106By similarity1

GO - Molecular functioni

GO - Biological processi

  • nitrogen utilization Source: UniProtKB
  • pyrimidine nucleobase catabolic process Source: EcoCyc
  • uracil catabolic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase

Enzyme and pathway databases

BioCyciEcoCyc:G6521-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative aminoacrylate peracid reductase RutC (EC:1.-.-.-)
Gene namesi
Name:rutC
Synonyms:ycdK
Ordered Locus Names:b1010, JW0995
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13857 rutC

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to grow on uridine as the sole source of nitrogen at room temperature indicating a probably accumulation of a toxic intermediate, the peracid aminoacrylate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001703191 – 128Putative aminoacrylate peracid reductase RutCAdd BLAST128

Proteomic databases

PaxDbiP0AFQ5
PRIDEiP0AFQ5

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.2 Publications

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

BioGridi4260036, 13 interactors
IntActiP0AFQ5, 7 interactors
STRINGi316385.ECDH10B_1082

Structurei

3D structure databases

ProteinModelPortaliP0AFQ5
SMRiP0AFQ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RutC family.Curated

Phylogenomic databases

eggNOGiENOG4107YYT Bacteria
COG0251 LUCA
HOGENOMiHOG000267215
InParanoidiP0AFQ5
KOiK09021
OMAiVYCSGNI
PhylomeDBiP0AFQ5

Family and domain databases

Gene3Di3.30.1330.40, 1 hit
HAMAPiMF_00831 RutC, 1 hit
InterProiView protein in InterPro
IPR019897 RidA_CS
IPR019898 RutC
IPR035959 RutC-like_sf
IPR006175 YjgF/YER057c/UK114
PANTHERiPTHR11803 PTHR11803, 1 hit
PfamiView protein in Pfam
PF01042 Ribonuc_L-PSP, 1 hit
SUPFAMiSSF55298 SSF55298, 1 hit
TIGRFAMsiTIGR03610 RutC, 1 hit
PROSITEiView protein in PROSITE
PS01094 UPF0076, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKSVIIPAG SSAPLAPFVP GTLADGVVYV SGTLAFDQHN NVLFADDPKA
60 70 80 90 100
QTRHVLETIR KVIETAGGTM ADVTFNSIFI TDWKNYAAIN EIYAEFFPGD
110 120
KPARFCIQCG LVKPDALVEI ATIAHIAK
Length:128
Mass (Da):13,763
Last modified:December 20, 2005 - v1
Checksum:i7D5FC2374597D907
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74095.1
AP009048 Genomic DNA Translation: BAA35777.1
PIRiH64842
RefSeqiNP_415530.1, NC_000913.3
WP_001126780.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74095; AAC74095; b1010
BAA35777; BAA35777; BAA35777
GeneIDi945599
KEGGiecj:JW0995
eco:b1010
PATRICifig|1411691.4.peg.1261

Similar proteinsi

Entry informationi

Entry nameiRUTC_ECOLI
AccessioniPrimary (citable) accession number: P0AFQ5
Secondary accession number(s): P75896
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health