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Protein

DNA base-flipping protein

Gene

atl

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in DNA damage recognition. Binds DNA containing O6-methylguanine and larger O6-alkylguanine adducts, and to double-stranded DNA that contains an AP (apurinic/apyrimidinic) site (PubMed:16027108, PubMed:18084297, PubMed:19269902, PubMed:20921378). Binds to the damaged base and flips the base out of the DNA duplex into an extrahelical conformation, which allows processing by repair proteins (PubMed:18084297). Works in partnership with the nucleotide excision repair (NER) pathway to enhance the repair of the O6-alkylguanine adducts larger than the methyl adduct (PubMed:19269902, PubMed:20921378). Also prevents methyl-directed mismatch repair (MMR)-mediated attack of the O6-alkylguanine:T mispairs for the larger alkyl groups (PubMed:20921378).4 Publications

Miscellaneous

Does not have alkyltransferase activity. A tryptophan residue replaces the cysteine at the known active site of MGMT.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei52Required for phosphate rotation/nucleotide flippingBy similarity1
Sitei66Arg finger, required for nucleotide flippingBy similarity1

GO - Molecular functioni

  • catalytic activity Source: InterPro
  • damaged DNA binding Source: EcoCyc
  • enzyme binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage

Enzyme and pathway databases

BioCyciEcoCyc:G6251-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA base-flipping protein1 Publication
Alternative name(s):
Alkyltransferase-like protein ATL1 Publication
Gene namesi
Name:atl1 Publication
Synonyms:ybaZ1 Publication
Ordered Locus Names:b0454, JW0444
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13254 ybaZ

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi83W → C: Does not confer alkyltransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393901 – 129DNA base-flipping proteinAdd BLAST129

Proteomic databases

PaxDbiP0AFP2
PRIDEiP0AFP2

Interactioni

Subunit structurei

Interacts with HelD and UvrA.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
uvrAP0A6982EBI-560039,EBI-552091

GO - Molecular functioni

  • enzyme binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4259845, 66 interactors
DIPiDIP-48135N
IntActiP0AFP2, 16 interactors
STRINGi316385.ECDH10B_0410

Structurei

3D structure databases

ProteinModelPortaliP0AFP2
SMRiP0AFP2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family. ATL subfamily.Curated

Phylogenomic databases

eggNOGiENOG41080UX Bacteria
COG3695 LUCA
HOGENOMiHOG000244138
InParanoidiP0AFP2
KOiK07443
OMAiWHRVINA
PhylomeDBiP0AFP2

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVSCAMRLH SGVFPDYAEK LPQEEKMEKE DSFPQRVWQI VAAIPEGYVT
60 70 80 90 100
TYGDVAKLAG SPRAARQVGG VLKRLPEGST LPWHRVVNRH GTISLTGPDL
110 120
QRQRQALLAE GVMVSGSGQI DLQRYRWNY
Length:129
Mass (Da):14,450
Last modified:December 20, 2005 - v1
Checksum:i584382A5657919CA
GO

Sequence cautioni

The sequence AAB40210 differs from that shown. Reason: Frameshift at position 64.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82664 Genomic DNA Translation: AAB40210.1 Frameshift.
U00096 Genomic DNA Translation: AAC73557.1
AP009048 Genomic DNA Translation: BAE76234.1
PIRiF64775
RefSeqiNP_414988.1, NC_000913.3
WP_001300427.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73557; AAC73557; b0454
BAE76234; BAE76234; BAE76234
GeneIDi945094
KEGGiecj:JW0444
eco:b0454
PATRICifig|511145.12.peg.473

Entry informationi

Entry nameiATL_ECOLI
AccessioniPrimary (citable) accession number: P0AFP2
Secondary accession number(s): P75707, P77119, Q2MBX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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