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Protein

DNA base-flipping protein

Gene

atl

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA damage recognition. Binds DNA containing O(6)-methylguanine and larger O(6)-alkylguanine adducts, and to double-stranded DNA that contains an AP (apurinic/apyrimidinic) site (PubMed:16027108, PubMed:18084297, PubMed:19269902, PubMed:20921378). Binds to the damaged base and flips the base out of the DNA duplex into an extrahelical conformation, which allows processing by repair proteins (PubMed:18084297). Works in partnership with the nucleotide excision repair (NER) pathway to enhance the repair of the O(6)-alkylguanine adducts larger than the methyl adduct (PubMed:19269902, PubMed:20921378). Also prevents methyl-directed mismatch repair (MMR)-mediated attack of the O(6)-alkylguanine:T mispairs for the larger alkyl groups (PubMed:20921378).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei52Required for phosphate rotation/nucleotide flippingBy similarity1
Sitei66Arg finger, required for nucleotide flippingBy similarity1

GO - Molecular functioni

  • catalytic activity Source: InterPro
  • damaged DNA binding Source: EcoCyc
  • enzyme binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6251-MONOMER.
ECOL316407:JW0444-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA base-flipping protein1 Publication
Alternative name(s):
Alkyltransferase-like protein ATL1 Publication
Gene namesi
Name:atl1 Publication
Synonyms:ybaZ1 Publication
Ordered Locus Names:b0454, JW0444
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13254. ybaZ.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi83W → C: Does not confer alkyltransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393901 – 129DNA base-flipping proteinAdd BLAST129

Proteomic databases

PaxDbiP0AFP2.
PRIDEiP0AFP2.

Interactioni

Subunit structurei

Interacts with HelD and UvrA.2 Publications

GO - Molecular functioni

  • enzyme binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4259845. 66 interactors.
DIPiDIP-48135N.
IntActiP0AFP2. 13 interactors.
MINTiMINT-1239891.
STRINGi511145.b0454.

Structurei

3D structure databases

ProteinModelPortaliP0AFP2.
SMRiP0AFP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family. ATL subfamily.Curated

Phylogenomic databases

eggNOGiENOG41080UX. Bacteria.
COG3695. LUCA.
HOGENOMiHOG000244138.
InParanoidiP0AFP2.
KOiK07443.
OMAiAVIHQIP.
PhylomeDBiP0AFP2.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AFP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVSCAMRLH SGVFPDYAEK LPQEEKMEKE DSFPQRVWQI VAAIPEGYVT
60 70 80 90 100
TYGDVAKLAG SPRAARQVGG VLKRLPEGST LPWHRVVNRH GTISLTGPDL
110 120
QRQRQALLAE GVMVSGSGQI DLQRYRWNY
Length:129
Mass (Da):14,450
Last modified:December 20, 2005 - v1
Checksum:i584382A5657919CA
GO

Sequence cautioni

The sequence AAB40210 differs from that shown. Reason: Frameshift at position 64.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82664 Genomic DNA. Translation: AAB40210.1. Frameshift.
U00096 Genomic DNA. Translation: AAC73557.1.
AP009048 Genomic DNA. Translation: BAE76234.1.
PIRiF64775.
RefSeqiNP_414988.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73557; AAC73557; b0454.
BAE76234; BAE76234; BAE76234.
GeneIDi945094.
KEGGiecj:JW0444.
eco:b0454.
PATRICi32116063. VBIEscCol129921_0473.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82664 Genomic DNA. Translation: AAB40210.1. Frameshift.
U00096 Genomic DNA. Translation: AAC73557.1.
AP009048 Genomic DNA. Translation: BAE76234.1.
PIRiF64775.
RefSeqiNP_414988.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0AFP2.
SMRiP0AFP2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259845. 66 interactors.
DIPiDIP-48135N.
IntActiP0AFP2. 13 interactors.
MINTiMINT-1239891.
STRINGi511145.b0454.

Proteomic databases

PaxDbiP0AFP2.
PRIDEiP0AFP2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73557; AAC73557; b0454.
BAE76234; BAE76234; BAE76234.
GeneIDi945094.
KEGGiecj:JW0444.
eco:b0454.
PATRICi32116063. VBIEscCol129921_0473.

Organism-specific databases

EchoBASEiEB3043.
EcoGeneiEG13254. ybaZ.

Phylogenomic databases

eggNOGiENOG41080UX. Bacteria.
COG3695. LUCA.
HOGENOMiHOG000244138.
InParanoidiP0AFP2.
KOiK07443.
OMAiAVIHQIP.
PhylomeDBiP0AFP2.

Enzyme and pathway databases

BioCyciEcoCyc:G6251-MONOMER.
ECOL316407:JW0444-MONOMER.

Miscellaneous databases

PROiP0AFP2.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiATL_ECOLI
AccessioniPrimary (citable) accession number: P0AFP2
Secondary accession number(s): P75707, P77119, Q2MBX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Does not have alkyltransferase activity. A tryptophan residue replaces the cysteine at the known active site of MGMT.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.