P0AFM9 (PSPB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phage shock protein B

Gene names

Name:	pspB
Ordered Locus Names:	b1305, JW1298

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	74 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspB is involved in transcription regulation. Ref.1
Subunit structure	Interacts with PspA; could bind PspA only in the presence of PspC. Ref.5
Subcellular location	Cell inner membrane; Single-pass membrane protein.
Induction	By heat, ethanol, osmotic shock and infection by filamentous bacteriophages. Ref.1
Sequence similarities	Belongs to the PspB family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phage shock Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from experiment PubMed 1717346. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 74	74	Phage shock protein B		PRO_0000097071
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Coiled coil	33 – 68	36	Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AFM9 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 22B82DF367099A1B	FASTA	74	8,763
	10 20 30 40 50 60 MSALFLAIPL TIFVLFVLPI WLWLHYSNRS GRSELSQSEQ QRLAQLADEA KRMRERIQAL 70 ESILDAEHPN WRDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and sequence of the Escherichia coli stress-induced psp operon." Brissette J.L., Weiner L., Ripmaster T.L., Model P. J. Mol. Biol. 220:35-48(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Interactions between phage-shock proteins in Escherichia coli." Adams H., Teertstra W., Demmers J., Boesten R., Tommassen J. J. Bacteriol. 185:1174-1180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PSPA. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57560 Genomic DNA. Translation: CAA40790.1. U00096 Genomic DNA. Translation: AAC74387.1. AP009048 Genomic DNA. Translation: BAA14874.1.
PIR	S17122.
RefSeq	NP_415821.1. NC_000913.2. YP_489573.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AFM9.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AFM9. 1 interaction.
STRING	511145.b1305.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74387; AAC74387; b1305. BAA14874; BAA14874; BAA14874.
GeneID	12934239. 945893.
KEGG	ecj:Y75_p1280. eco:b1305.
PATRIC	32117882. VBIEscCol129921_1361.
Organism-specific databases
EchoBASE	EB0770.
EcoGene	EG10777. pspB.
Phylogenomic databases
eggNOG	NOG08124.
HOGENOM	HOG000276514.
KO	K03970.
OMA	IWLILHY.
ProtClustDB	PRK09458.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10777-MONOMER. ECOL316407:JW1298-MONOMER.
Gene expression databases
Genevestigator	P0AFM9.
Family and domain databases
InterPro	IPR009554. Phageshock_PspB. [Graphical view]
Pfam	PF06667. PspB. 1 hit. [Graphical view]
TIGRFAMs	TIGR02976. phageshock_pspB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PSPB_ECOLI

Accession

Primary (citable) accession number: P0AFM9
Secondary accession number(s): P23854

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program