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Protein

Glycine betaine/proline betaine-binding periplasmic protein

Gene

proX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ProU ABC transporter complex involved in glycine betaine and proline betaine uptake. Binds glycine betaine and proline betaine with high affinity.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861Substrate1 Publication
Binding sitei90 – 901Substrate1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PROX-MONOMER.
ECOL316407:JW2654-MONOMER.
MetaCyc:PROX-MONOMER.

Protein family/group databases

TCDBi3.A.1.12.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine betaine/proline betaine-binding periplasmic proteinCurated
Alternative name(s):
GBBP1 Publication
Gene namesi
Name:proX1 Publication
Synonyms:proU1 Publication
Ordered Locus Names:b2679, JW2654
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10773. proX.

Subcellular locationi

  • Periplasm 2 Publications

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 330309Glycine betaine/proline betaine-binding periplasmic proteinPRO_0000031847Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi157 ↔ 1631 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0AFM2.
PRIDEiP0AFM2.

2D gel databases

SWISS-2DPAGEP0AFM2.

Expressioni

Inductioni

By cold shock in a PNPase-dependent fashion.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (ProV), two transmembrane proteins (ProW) and a solute-binding protein (ProX).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
yagTP771652EBI-1129961,EBI-1115563

Protein-protein interaction databases

BioGridi4262270. 7 interactions.
IntActiP0AFM2. 2 interactions.
STRINGi511145.b2679.

Structurei

Secondary structure

1
330
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni25 – 284Combined sources
Beta strandi33 – 353Combined sources
Helixi39 – 413Combined sources
Helixi42 – 5413Combined sources
Helixi67 – 7610Combined sources
Beta strandi77 – 793Combined sources
Beta strandi81 – 877Combined sources
Turni88 – 903Combined sources
Helixi91 – 966Combined sources
Helixi99 – 1013Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi109 – 12012Combined sources
Helixi121 – 1277Combined sources
Helixi132 – 1365Combined sources
Helixi138 – 1414Combined sources
Helixi142 – 1443Combined sources
Beta strandi146 – 15510Combined sources
Helixi162 – 17312Combined sources
Turni177 – 1793Combined sources
Beta strandi180 – 1834Combined sources
Helixi187 – 19913Combined sources
Beta strandi205 – 2139Combined sources
Helixi214 – 2174Combined sources
Turni220 – 2223Combined sources
Beta strandi223 – 2264Combined sources
Beta strandi254 – 2629Combined sources
Helixi263 – 2686Combined sources
Helixi270 – 2789Combined sources
Helixi283 – 29513Combined sources
Helixi300 – 31314Combined sources
Helixi315 – 32612Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R9LX-ray1.59A22-330[»]
1R9QX-ray2.05A22-330[»]
ProteinModelPortaliP0AFM2.
SMRiP0AFM2. Positions 22-330.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFM2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 1633Substrate binding1 Publication

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105GIJ. Bacteria.
COG2113. LUCA.
HOGENOMiHOG000282038.
InParanoidiP0AFM2.
KOiK02002.
OMAiTAEKYHI.
PhylomeDBiP0AFM2.

Family and domain databases

InterProiIPR007210. ABC_Gly_betaine_transp_sub-bd.
[Graphical view]
PfamiPF04069. OpuAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHSVLFATA FATLISTQTF AADLPGKGIT VNPVQSTITE ETFQTLLVSR
60 70 80 90 100
ALEKLGYTVN KPSEVDYNVG YTSLASGDAT FTAVNWTPLH DNMYEAAGGD
110 120 130 140 150
KKFYREGVFV NGAAQGYLID KKTADQYKIT NIAQLKDPKI AKLFDTNGDG
160 170 180 190 200
KADLTGCNPG WGCEGAINHQ LAAYELTNTV THNQGNYAAM MADTISRYKE
210 220 230 240 250
GKPVFYYTWT PYWVSNELKP GKDVVWLQVP FSALPGDKNA DTKLPNGANY
260 270 280 290 300
GFPVSTMHIV ANKAWAEKNP AAAKLFAIMQ LPVADINAQN AIMHDGKASE
310 320 330
GDIQGHVDGW IKAHQQQFDG WVNEALAAQK
Length:330
Mass (Da):36,023
Last modified:December 20, 2005 - v1
Checksum:i2B98AF52552ACD2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24856 Genomic DNA. Translation: AAA24429.1.
U00096 Genomic DNA. Translation: AAC75726.1.
AP009048 Genomic DNA. Translation: BAA16544.1.
PIRiJS0130. BLECGP.
RefSeqiNP_417165.1. NC_000913.3.
WP_001216525.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75726; AAC75726; b2679.
BAA16544; BAA16544; BAA16544.
GeneIDi947165.
KEGGiecj:JW2654.
eco:b2679.
PATRICi32120748. VBIEscCol129921_2771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24856 Genomic DNA. Translation: AAA24429.1.
U00096 Genomic DNA. Translation: AAC75726.1.
AP009048 Genomic DNA. Translation: BAA16544.1.
PIRiJS0130. BLECGP.
RefSeqiNP_417165.1. NC_000913.3.
WP_001216525.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R9LX-ray1.59A22-330[»]
1R9QX-ray2.05A22-330[»]
ProteinModelPortaliP0AFM2.
SMRiP0AFM2. Positions 22-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262270. 7 interactions.
IntActiP0AFM2. 2 interactions.
STRINGi511145.b2679.

Protein family/group databases

TCDBi3.A.1.12.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP0AFM2.

Proteomic databases

PaxDbiP0AFM2.
PRIDEiP0AFM2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75726; AAC75726; b2679.
BAA16544; BAA16544; BAA16544.
GeneIDi947165.
KEGGiecj:JW2654.
eco:b2679.
PATRICi32120748. VBIEscCol129921_2771.

Organism-specific databases

EchoBASEiEB0766.
EcoGeneiEG10773. proX.

Phylogenomic databases

eggNOGiENOG4105GIJ. Bacteria.
COG2113. LUCA.
HOGENOMiHOG000282038.
InParanoidiP0AFM2.
KOiK02002.
OMAiTAEKYHI.
PhylomeDBiP0AFM2.

Enzyme and pathway databases

BioCyciEcoCyc:PROX-MONOMER.
ECOL316407:JW2654-MONOMER.
MetaCyc:PROX-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFM2.
PROiP0AFM2.

Family and domain databases

InterProiIPR007210. ABC_Gly_betaine_transp_sub-bd.
[Graphical view]
PfamiPF04069. OpuAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROX_ECOLI
AccessioniPrimary (citable) accession number: P0AFM2
Secondary accession number(s): P14177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.