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Protein

Glycine betaine/proline betaine-binding periplasmic protein

Gene

proX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ProU ABC transporter complex involved in glycine betaine and proline betaine uptake. Binds glycine betaine and proline betaine with high affinity.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86Substrate1 Publication1
Binding sitei90Substrate1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PROX-MONOMER.
ECOL316407:JW2654-MONOMER.
MetaCyc:PROX-MONOMER.

Protein family/group databases

TCDBi3.A.1.12.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine betaine/proline betaine-binding periplasmic proteinCurated
Alternative name(s):
GBBP1 Publication
Gene namesi
Name:proX1 Publication
Synonyms:proU1 Publication
Ordered Locus Names:b2679, JW2654
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10773. proX.

Subcellular locationi

  • Periplasm 2 Publications

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000003184722 – 330Glycine betaine/proline betaine-binding periplasmic proteinAdd BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi157 ↔ 1631 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0AFM2.
PRIDEiP0AFM2.

2D gel databases

SWISS-2DPAGEP0AFM2.

Expressioni

Inductioni

By cold shock in a PNPase-dependent fashion.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (ProV), two transmembrane proteins (ProW) and a solute-binding protein (ProX).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
yagTP771652EBI-1129961,EBI-1115563

Protein-protein interaction databases

BioGridi4262270. 7 interactors.
IntActiP0AFM2. 2 interactors.
STRINGi511145.b2679.

Structurei

Secondary structure

1330
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni25 – 28Combined sources4
Beta strandi33 – 35Combined sources3
Helixi39 – 41Combined sources3
Helixi42 – 54Combined sources13
Helixi67 – 76Combined sources10
Beta strandi77 – 79Combined sources3
Beta strandi81 – 87Combined sources7
Turni88 – 90Combined sources3
Helixi91 – 96Combined sources6
Helixi99 – 101Combined sources3
Beta strandi103 – 105Combined sources3
Beta strandi109 – 120Combined sources12
Helixi121 – 127Combined sources7
Helixi132 – 136Combined sources5
Helixi138 – 141Combined sources4
Helixi142 – 144Combined sources3
Beta strandi146 – 155Combined sources10
Helixi162 – 173Combined sources12
Turni177 – 179Combined sources3
Beta strandi180 – 183Combined sources4
Helixi187 – 199Combined sources13
Beta strandi205 – 213Combined sources9
Helixi214 – 217Combined sources4
Turni220 – 222Combined sources3
Beta strandi223 – 226Combined sources4
Beta strandi254 – 262Combined sources9
Helixi263 – 268Combined sources6
Helixi270 – 278Combined sources9
Helixi283 – 295Combined sources13
Helixi300 – 313Combined sources14
Helixi315 – 326Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R9LX-ray1.59A22-330[»]
1R9QX-ray2.05A22-330[»]
ProteinModelPortaliP0AFM2.
SMRiP0AFM2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFM2.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni161 – 163Substrate binding1 Publication3

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105GIJ. Bacteria.
COG2113. LUCA.
HOGENOMiHOG000282038.
InParanoidiP0AFM2.
KOiK02002.
OMAiTAEKYHI.
PhylomeDBiP0AFM2.

Family and domain databases

InterProiIPR007210. ABC_Gly_betaine_transp_sub-bd.
[Graphical view]
PfamiPF04069. OpuAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHSVLFATA FATLISTQTF AADLPGKGIT VNPVQSTITE ETFQTLLVSR
60 70 80 90 100
ALEKLGYTVN KPSEVDYNVG YTSLASGDAT FTAVNWTPLH DNMYEAAGGD
110 120 130 140 150
KKFYREGVFV NGAAQGYLID KKTADQYKIT NIAQLKDPKI AKLFDTNGDG
160 170 180 190 200
KADLTGCNPG WGCEGAINHQ LAAYELTNTV THNQGNYAAM MADTISRYKE
210 220 230 240 250
GKPVFYYTWT PYWVSNELKP GKDVVWLQVP FSALPGDKNA DTKLPNGANY
260 270 280 290 300
GFPVSTMHIV ANKAWAEKNP AAAKLFAIMQ LPVADINAQN AIMHDGKASE
310 320 330
GDIQGHVDGW IKAHQQQFDG WVNEALAAQK
Length:330
Mass (Da):36,023
Last modified:December 20, 2005 - v1
Checksum:i2B98AF52552ACD2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24856 Genomic DNA. Translation: AAA24429.1.
U00096 Genomic DNA. Translation: AAC75726.1.
AP009048 Genomic DNA. Translation: BAA16544.1.
PIRiJS0130. BLECGP.
RefSeqiNP_417165.1. NC_000913.3.
WP_001216525.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75726; AAC75726; b2679.
BAA16544; BAA16544; BAA16544.
GeneIDi947165.
KEGGiecj:JW2654.
eco:b2679.
PATRICi32120748. VBIEscCol129921_2771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24856 Genomic DNA. Translation: AAA24429.1.
U00096 Genomic DNA. Translation: AAC75726.1.
AP009048 Genomic DNA. Translation: BAA16544.1.
PIRiJS0130. BLECGP.
RefSeqiNP_417165.1. NC_000913.3.
WP_001216525.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R9LX-ray1.59A22-330[»]
1R9QX-ray2.05A22-330[»]
ProteinModelPortaliP0AFM2.
SMRiP0AFM2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262270. 7 interactors.
IntActiP0AFM2. 2 interactors.
STRINGi511145.b2679.

Protein family/group databases

TCDBi3.A.1.12.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP0AFM2.

Proteomic databases

PaxDbiP0AFM2.
PRIDEiP0AFM2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75726; AAC75726; b2679.
BAA16544; BAA16544; BAA16544.
GeneIDi947165.
KEGGiecj:JW2654.
eco:b2679.
PATRICi32120748. VBIEscCol129921_2771.

Organism-specific databases

EchoBASEiEB0766.
EcoGeneiEG10773. proX.

Phylogenomic databases

eggNOGiENOG4105GIJ. Bacteria.
COG2113. LUCA.
HOGENOMiHOG000282038.
InParanoidiP0AFM2.
KOiK02002.
OMAiTAEKYHI.
PhylomeDBiP0AFM2.

Enzyme and pathway databases

BioCyciEcoCyc:PROX-MONOMER.
ECOL316407:JW2654-MONOMER.
MetaCyc:PROX-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFM2.
PROiP0AFM2.

Family and domain databases

InterProiIPR007210. ABC_Gly_betaine_transp_sub-bd.
[Graphical view]
PfamiPF04069. OpuAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROX_ECOLI
AccessioniPrimary (citable) accession number: P0AFM2
Secondary accession number(s): P14177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.