P0AFL9 (PQIA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Paraquat-inducible protein A

Gene names

Name:	pqiA
Synonyms:	pqi5A
Ordered Locus Names:	b0950, JW0933

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Induction	By paraquat.
Sequence similarities	Belongs to the PqiA family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from direct assay Ref.1. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Paraquat-inducible protein A		PRO_0000058554
Regions
	Transmembrane	51 – 71	21	Helical; Potential
	Transmembrane	102 – 122	21	Helical; Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Transmembrane	257 – 277	21	Helical; Potential
	Transmembrane	285 – 305	21	Helical; Potential
	Transmembrane	306 – 326	21	Helical; Potential
	Transmembrane	347 – 367	21	Helical; Potential
	Transmembrane	372 – 392	21	Helical; Potential
Experimental info
	Sequence conflict	338 – 417	80	SERMH…EHEES → RKECI in CAA57256. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0AFL9 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 959FD92C83F3BC8D	FASTA	417	46,463
	10 20 30 40 50 60 MCEHHHAAKH ILCSQCDMLV ALPRLEHGQK AACPRCGTTL TVAWDAPRQR PTAYALAALF 70 80 90 100 110 120 MLLLSNLFPF VNMNVAGVTS EITLLEIPGV LFSEDYASLG TFFLLFVQLV PAFCLITILL 130 140 150 160 170 180 LVNRAELPVR LKEQLARVLF QLKTWGMAEI FLAGVLVSFV KLMAYGSIGV GSSFLPWCLF 190 200 210 220 230 240 CVLQLRAFQC VDRRWLWDDI APMPELRQPL KPGVTGIRQG LRSCSCCTAI LPADEPVCPR 250 260 270 280 290 300 CSTKGYVRRR NSLQWTLALL VTSIMLYLPA NILPIMVTDL LGSKMPSTIL AGVILLWSEG 310 320 330 340 350 360 SYPVAAVIFL ASIMVPTLKM IAIAWLCWDA KGHGKRDSER MHLIYEVVEF VGRWSMIDVF 370 380 390 400 410 VIAVLSALVR MGGLMSIYPA MGALMFALVV IMTMFSAMTF DPRLSWDRQP ESEHEES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a novel paraquat-inducible (pqi) gene regulated by the soxRS locus in Escherichia coli." Koh Y.-S., Roe J.-H. J. Bacteriol. 177:2673-2678(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X81561 Genomic DNA. Translation: CAA57256.1. U00096 Genomic DNA. Translation: AAC74036.1. AP009048 Genomic DNA. Translation: BAA35708.1.
PIR	E64835.
RefSeq	NP_415470.1. NC_000913.2. YP_489222.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AFL9.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AFL9. 2 interactions.
STRING	511145.b0950.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74036; AAC74036; b0950. BAA35708; BAA35708; BAA35708.
GeneID	12931033. 944999.
KEGG	ecj:Y75_p0922. eco:b0950.
PATRIC	32117119. VBIEscCol129921_0984.
Organism-specific databases
EchoBASE	EB2794.
EcoGene	EG12965. pqiA.
Phylogenomic databases
eggNOG	COG2995.
HOGENOM	HOG000283618.
KO	K03808.
OMA	MIDIFVI.
ProtClustDB	PRK15103.
Enzyme and pathway databases
BioCyc	EcoCyc:G6490-MONOMER. ECOL316407:JW0933-MONOMER.
Gene expression databases
Genevestigator	P0AFL9.
Family and domain databases
InterPro	IPR005219. CHP00155. IPR007498. PqiA. [Graphical view]
Pfam	PF04403. PqiA. 2 hits. [Graphical view]
TIGRFAMs	TIGR00155. pqiA_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PQIA_ECOLI

Accession

Primary (citable) accession number: P0AFL9
Secondary accession number(s): P43670, P77566

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program