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P0AFL8

- PPX_ECO57

UniProt

P0AFL8 - PPX_ECO57

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Protein
Exopolyphosphatase
Gene
ppx, Z3765, ECs3364
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate By similarity.

Catalytic activityi

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

Cofactori

Magnesium By similarity.

GO - Molecular functioni

  1. exopolyphosphatase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. polyphosphate catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3326-MONOMER.
ECOO157:PPX-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolyphosphatase (EC:3.6.1.11)
Short name:
ExopolyPase
Alternative name(s):
Metaphosphatase
Gene namesi
Name:ppx
Ordered Locus Names:Z3765, ECs3364
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 513512Exopolyphosphatase
PRO_0000194300Add
BLAST

Proteomic databases

PRIDEiP0AFL8.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi155864.Z3765.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 187
Beta strandi20 – 3112
Beta strandi34 – 4411
Helixi48 – 503
Helixi59 – 7517
Turni76 – 783
Helixi81 – 833
Beta strandi84 – 885
Helixi90 – 945
Helixi98 – 1058
Turni106 – 1083
Beta strandi113 – 1153
Helixi118 – 13114
Beta strandi139 – 1446
Beta strandi149 – 1557
Beta strandi158 – 1669
Helixi169 – 1768
Helixi178 – 1803
Helixi184 – 19613
Turni197 – 2015
Helixi202 – 2087
Beta strandi211 – 2177
Helixi218 – 22811
Turni229 – 2313
Helixi239 – 25012
Helixi255 – 2573
Helixi265 – 2684
Helixi271 – 28515
Beta strandi290 – 2923
Helixi297 – 30913
Helixi314 – 32613
Helixi330 – 35021
Turni352 – 3543
Helixi357 – 36913
Helixi372 – 3754
Helixi381 – 39111
Helixi399 – 41012
Beta strandi412 – 4143
Helixi418 – 4203
Beta strandi425 – 4273
Helixi429 – 44416
Turni445 – 4484
Helixi449 – 4513
Beta strandi459 – 4635
Beta strandi466 – 4716
Helixi475 – 4773
Helixi480 – 49415
Beta strandi500 – 5056

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FLOX-ray2.20A/B/C/D2-513[»]
ProteinModelPortaliP0AFL8.

Miscellaneous databases

EvolutionaryTraceiP0AFL8.

Family & Domainsi

Sequence similaritiesi

Belongs to the GppA/Ppx family.

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiENNKLLH.
OrthoDBiEOG6M6JN6.

Family and domain databases

InterProiIPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03706. exo_poly_only. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFL8-1 [UniParc]FASTAAdd to Basket

« Hide

MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG    50
LGPDNMLSEE AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD 100
FLKRAEKVIP YPIEIISGNE EARLIFMGVE HTQPEKGRKL VIDIGGGSTE 150
LVIGENFEPI LVESRRMGCV SFAQLYFPGG VINKENFQRA RMAAAQKLET 200
LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE RLEKLVKEVL 250
RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG 300
VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ 350
QPKLAHPQLE ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE 400
QQLMMATLVR YHRKAIKLDD LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ 450
ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA LVLLDLEKEQ EYWEGVAGWR 500
LKIEEESTPE IAA 513
Length:513
Mass (Da):58,136
Last modified:January 23, 2007 - v2
Checksum:i58E58E0F5D9FB9C3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG57612.1.
BA000007 Genomic DNA. Translation: BAB36787.1.
PIRiD91049.
H85893.
RefSeqiNP_289055.1. NC_002655.2.
NP_311391.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG57612; AAG57612; Z3765.
BAB36787; BAB36787; BAB36787.
GeneIDi914211.
957770.
KEGGiece:Z3765.
ecs:ECs3364.
PATRICi18356150. VBIEscCol44059_3270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG57612.1 .
BA000007 Genomic DNA. Translation: BAB36787.1 .
PIRi D91049.
H85893.
RefSeqi NP_289055.1. NC_002655.2.
NP_311391.1. NC_002695.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FLO X-ray 2.20 A/B/C/D 2-513 [» ]
ProteinModelPortali P0AFL8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z3765.

Proteomic databases

PRIDEi P0AFL8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG57612 ; AAG57612 ; Z3765 .
BAB36787 ; BAB36787 ; BAB36787 .
GeneIDi 914211.
957770.
KEGGi ece:Z3765.
ecs:ECs3364.
PATRICi 18356150. VBIEscCol44059_3270.

Phylogenomic databases

eggNOGi COG0248.
HOGENOMi HOG000258672.
KOi K01524.
OMAi ENNKLLH.
OrthoDBi EOG6M6JN6.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-3326-MONOMER.
ECOO157:PPX-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AFL8.

Family and domain databases

InterProi IPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
[Graphical view ]
Pfami PF02541. Ppx-GppA. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR03706. exo_poly_only. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiPPX_ECO57
AccessioniPrimary (citable) accession number: P0AFL8
Secondary accession number(s): P29014, P76981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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