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Protein

Exopolyphosphatase

Gene

ppx

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate (By similarity).By similarity

Catalytic activityi

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciECOO157:PPX-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolyphosphatase (EC:3.6.1.11)
Short name:
ExopolyPase
Alternative name(s):
Metaphosphatase
Gene namesi
Name:ppx
Ordered Locus Names:Z3765, ECs3364
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001943002 – 513ExopolyphosphataseAdd BLAST512

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi155864.Z3765.

Structurei

Secondary structure

1513
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 18Combined sources7
Beta strandi20 – 31Combined sources12
Beta strandi34 – 44Combined sources11
Helixi48 – 50Combined sources3
Helixi59 – 75Combined sources17
Turni76 – 78Combined sources3
Helixi81 – 83Combined sources3
Beta strandi84 – 88Combined sources5
Helixi90 – 94Combined sources5
Helixi98 – 105Combined sources8
Turni106 – 108Combined sources3
Beta strandi113 – 115Combined sources3
Helixi118 – 131Combined sources14
Beta strandi139 – 144Combined sources6
Beta strandi149 – 155Combined sources7
Beta strandi158 – 166Combined sources9
Helixi169 – 176Combined sources8
Helixi178 – 180Combined sources3
Helixi184 – 196Combined sources13
Turni197 – 201Combined sources5
Helixi202 – 208Combined sources7
Beta strandi211 – 217Combined sources7
Helixi218 – 228Combined sources11
Turni229 – 231Combined sources3
Helixi239 – 250Combined sources12
Helixi255 – 257Combined sources3
Helixi265 – 268Combined sources4
Helixi271 – 285Combined sources15
Beta strandi290 – 292Combined sources3
Helixi297 – 309Combined sources13
Helixi314 – 326Combined sources13
Helixi330 – 350Combined sources21
Turni352 – 354Combined sources3
Helixi357 – 369Combined sources13
Helixi372 – 375Combined sources4
Helixi381 – 391Combined sources11
Helixi399 – 410Combined sources12
Beta strandi412 – 414Combined sources3
Helixi418 – 420Combined sources3
Beta strandi425 – 427Combined sources3
Helixi429 – 444Combined sources16
Turni445 – 448Combined sources4
Helixi449 – 451Combined sources3
Beta strandi459 – 463Combined sources5
Beta strandi466 – 471Combined sources6
Helixi475 – 477Combined sources3
Helixi480 – 494Combined sources15
Beta strandi500 – 505Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FLOX-ray2.20A/B/C/D2-513[»]
ProteinModelPortaliP0AFL8.
SMRiP0AFL8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFL8.

Family & Domainsi

Sequence similaritiesi

Belongs to the GppA/Ppx family.Curated

Phylogenomic databases

eggNOGiENOG4105C9X. Bacteria.
COG0248. LUCA.
HOGENOMiHOG000258672.
KOiK01524.
OMAiFETKSRV.

Family and domain databases

InterProiIPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.
TIGRFAMsiTIGR03706. exo_poly_only. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG
60 70 80 90 100
LGPDNMLSEE AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD
110 120 130 140 150
FLKRAEKVIP YPIEIISGNE EARLIFMGVE HTQPEKGRKL VIDIGGGSTE
160 170 180 190 200
LVIGENFEPI LVESRRMGCV SFAQLYFPGG VINKENFQRA RMAAAQKLET
210 220 230 240 250
LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE RLEKLVKEVL
260 270 280 290 300
RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG
310 320 330 340 350
VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ
360 370 380 390 400
QPKLAHPQLE ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE
410 420 430 440 450
QQLMMATLVR YHRKAIKLDD LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ
460 470 480 490 500
ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA LVLLDLEKEQ EYWEGVAGWR
510
LKIEEESTPE IAA
Length:513
Mass (Da):58,136
Last modified:January 23, 2007 - v2
Checksum:i58E58E0F5D9FB9C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57612.1.
BA000007 Genomic DNA. Translation: BAB36787.1.
PIRiD91049.
H85893.
RefSeqiNP_311391.1. NC_002695.1.
WP_001121363.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG57612; AAG57612; Z3765.
BAB36787; BAB36787; BAB36787.
GeneIDi914211.
KEGGiece:Z3765.
ecs:ECs3364.
PATRICi18356150. VBIEscCol44059_3270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57612.1.
BA000007 Genomic DNA. Translation: BAB36787.1.
PIRiD91049.
H85893.
RefSeqiNP_311391.1. NC_002695.1.
WP_001121363.1. NZ_LPWC02000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FLOX-ray2.20A/B/C/D2-513[»]
ProteinModelPortaliP0AFL8.
SMRiP0AFL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z3765.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG57612; AAG57612; Z3765.
BAB36787; BAB36787; BAB36787.
GeneIDi914211.
KEGGiece:Z3765.
ecs:ECs3364.
PATRICi18356150. VBIEscCol44059_3270.

Phylogenomic databases

eggNOGiENOG4105C9X. Bacteria.
COG0248. LUCA.
HOGENOMiHOG000258672.
KOiK01524.
OMAiFETKSRV.

Enzyme and pathway databases

BioCyciECOO157:PPX-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFL8.

Family and domain databases

InterProiIPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.
TIGRFAMsiTIGR03706. exo_poly_only. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPX_ECO57
AccessioniPrimary (citable) accession number: P0AFL8
Secondary accession number(s): P29014, P76981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.