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P0AFL8 (PPX_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Exopolyphosphatase
Short name=ExopolyPase
EC=3.6.1.11
Alternative name(s):
Metaphosphatase
Gene names
Name:ppx
Ordered Locus Names:Z3765, ECs3364
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate By similarity.

Catalytic activity

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

Cofactor

Magnesium By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the gppA/ppx family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   LigandMagnesium
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processpolyphosphate catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionexopolyphosphatase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 513512Exopolyphosphatase
PRO_0000194300

Secondary structure

1 ........................................................................................ 513
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AFL8 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 58E58E0F5D9FB9C3

FASTA51358,136
        10         20         30         40         50         60 
MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG LGPDNMLSEE 

        70         80         90        100        110        120 
AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD FLKRAEKVIP YPIEIISGNE 

       130        140        150        160        170        180 
EARLIFMGVE HTQPEKGRKL VIDIGGGSTE LVIGENFEPI LVESRRMGCV SFAQLYFPGG 

       190        200        210        220        230        240 
VINKENFQRA RMAAAQKLET LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE 

       250        260        270        280        290        300 
RLEKLVKEVL RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG 

       310        320        330        340        350        360 
VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ QPKLAHPQLE 

       370        380        390        400        410        420 
ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE QQLMMATLVR YHRKAIKLDD 

       430        440        450        460        470        480 
LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA 

       490        500        510 
LVLLDLEKEQ EYWEGVAGWR LKIEEESTPE IAA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG57612.1.
BA000007 Genomic DNA. Translation: BAB36787.1.
PIRD91049.
H85893.
RefSeqNP_289055.1. NC_002655.2.
NP_311391.1. NC_002695.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FLOX-ray2.20A/B/C/D2-512[»]
ProteinModelPortalP0AFL8.
SMRP0AFL8. Positions 12-511.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000025772; EBESCP00000024665; EBESCG00000024825.
EBESCT00000058003; EBESCP00000055831; EBESCG00000057051.
GeneID914211.
957770.
GenomeReviewsGene locus Z3765 in contig AE005174_GR.
Gene locus ECs3364 in contig BA000007_GR.
KEGGece:Z3765.
ecs:ECs3364.
PATRIC18356150. VBIEscCol44059_3270.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000009690.
HOGENOMHBG451789.
OMAHKHSYYL.
ProtClustDBPRK10854.

Enzyme and pathway databases

BioCycECOL83334:ECS3364-MONOMER.

Family and domain databases

InterProIPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
[Graphical view]
KOK01524.
PfamPF02541. Ppx-GppA. 1 hit.
[Graphical view]
TIGRFAMsTIGR03706. Exo_poly_only. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPX_ECO57
AccessionPrimary (citable) accession number: P0AFL8
Secondary accession number(s): P29014, P76981
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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