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P0AFL8

- PPX_ECO57

UniProt

P0AFL8 - PPX_ECO57

Protein

Exopolyphosphatase

Gene

ppx

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate By similarity.By similarity

    Catalytic activityi

    (Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

    Cofactori

    Magnesium.By similarity

    GO - Molecular functioni

    1. exopolyphosphatase activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. polyphosphate catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-3326-MONOMER.
    ECOO157:PPX-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exopolyphosphatase (EC:3.6.1.11)
    Short name:
    ExopolyPase
    Alternative name(s):
    Metaphosphatase
    Gene namesi
    Name:ppx
    Ordered Locus Names:Z3765, ECs3364
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 513512ExopolyphosphatasePRO_0000194300Add
    BLAST

    Proteomic databases

    PRIDEiP0AFL8.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi155864.Z3765.

    Structurei

    Secondary structure

    1
    513
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi12 – 187
    Beta strandi20 – 3112
    Beta strandi34 – 4411
    Helixi48 – 503
    Helixi59 – 7517
    Turni76 – 783
    Helixi81 – 833
    Beta strandi84 – 885
    Helixi90 – 945
    Helixi98 – 1058
    Turni106 – 1083
    Beta strandi113 – 1153
    Helixi118 – 13114
    Beta strandi139 – 1446
    Beta strandi149 – 1557
    Beta strandi158 – 1669
    Helixi169 – 1768
    Helixi178 – 1803
    Helixi184 – 19613
    Turni197 – 2015
    Helixi202 – 2087
    Beta strandi211 – 2177
    Helixi218 – 22811
    Turni229 – 2313
    Helixi239 – 25012
    Helixi255 – 2573
    Helixi265 – 2684
    Helixi271 – 28515
    Beta strandi290 – 2923
    Helixi297 – 30913
    Helixi314 – 32613
    Helixi330 – 35021
    Turni352 – 3543
    Helixi357 – 36913
    Helixi372 – 3754
    Helixi381 – 39111
    Helixi399 – 41012
    Beta strandi412 – 4143
    Helixi418 – 4203
    Beta strandi425 – 4273
    Helixi429 – 44416
    Turni445 – 4484
    Helixi449 – 4513
    Beta strandi459 – 4635
    Beta strandi466 – 4716
    Helixi475 – 4773
    Helixi480 – 49415
    Beta strandi500 – 5056

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FLOX-ray2.20A/B/C/D2-513[»]
    ProteinModelPortaliP0AFL8.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AFL8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GppA/Ppx family.Curated

    Phylogenomic databases

    eggNOGiCOG0248.
    HOGENOMiHOG000258672.
    KOiK01524.
    OMAiENNKLLH.
    OrthoDBiEOG6M6JN6.

    Family and domain databases

    InterProiIPR022371. Exopolyphosphatase.
    IPR003695. Ppx_GppA.
    [Graphical view]
    PfamiPF02541. Ppx-GppA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03706. exo_poly_only. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AFL8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG    50
    LGPDNMLSEE AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD 100
    FLKRAEKVIP YPIEIISGNE EARLIFMGVE HTQPEKGRKL VIDIGGGSTE 150
    LVIGENFEPI LVESRRMGCV SFAQLYFPGG VINKENFQRA RMAAAQKLET 200
    LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE RLEKLVKEVL 250
    RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG 300
    VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ 350
    QPKLAHPQLE ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE 400
    QQLMMATLVR YHRKAIKLDD LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ 450
    ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA LVLLDLEKEQ EYWEGVAGWR 500
    LKIEEESTPE IAA 513
    Length:513
    Mass (Da):58,136
    Last modified:January 23, 2007 - v2
    Checksum:i58E58E0F5D9FB9C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG57612.1.
    BA000007 Genomic DNA. Translation: BAB36787.1.
    PIRiD91049.
    H85893.
    RefSeqiNP_289055.1. NC_002655.2.
    NP_311391.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG57612; AAG57612; Z3765.
    BAB36787; BAB36787; BAB36787.
    GeneIDi914211.
    957770.
    KEGGiece:Z3765.
    ecs:ECs3364.
    PATRICi18356150. VBIEscCol44059_3270.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG57612.1 .
    BA000007 Genomic DNA. Translation: BAB36787.1 .
    PIRi D91049.
    H85893.
    RefSeqi NP_289055.1. NC_002655.2.
    NP_311391.1. NC_002695.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FLO X-ray 2.20 A/B/C/D 2-513 [» ]
    ProteinModelPortali P0AFL8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z3765.

    Proteomic databases

    PRIDEi P0AFL8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG57612 ; AAG57612 ; Z3765 .
    BAB36787 ; BAB36787 ; BAB36787 .
    GeneIDi 914211.
    957770.
    KEGGi ece:Z3765.
    ecs:ECs3364.
    PATRICi 18356150. VBIEscCol44059_3270.

    Phylogenomic databases

    eggNOGi COG0248.
    HOGENOMi HOG000258672.
    KOi K01524.
    OMAi ENNKLLH.
    OrthoDBi EOG6M6JN6.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-3326-MONOMER.
    ECOO157:PPX-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AFL8.

    Family and domain databases

    InterProi IPR022371. Exopolyphosphatase.
    IPR003695. Ppx_GppA.
    [Graphical view ]
    Pfami PF02541. Ppx-GppA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR03706. exo_poly_only. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiPPX_ECO57
    AccessioniPrimary (citable) accession number: P0AFL8
    Secondary accession number(s): P29014, P76981
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 60 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3