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P0AFL8

- PPX_ECO57

UniProt

P0AFL8 - PPX_ECO57

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Protein

Exopolyphosphatase

Gene

ppx

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate (By similarity).By similarity

Catalytic activityi

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

Cofactori

Mg2+By similarity

GO - Molecular functioni

  1. exopolyphosphatase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. polyphosphate catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3326-MONOMER.
ECOO157:PPX-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolyphosphatase (EC:3.6.1.11)
Short name:
ExopolyPase
Alternative name(s):
Metaphosphatase
Gene namesi
Name:ppx
Ordered Locus Names:Z3765, ECs3364
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Cell membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 513512ExopolyphosphatasePRO_0000194300Add
BLAST

Proteomic databases

PRIDEiP0AFL8.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi155864.Z3765.

Structurei

Secondary structure

1
513
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 187Combined sources
Beta strandi20 – 3112Combined sources
Beta strandi34 – 4411Combined sources
Helixi48 – 503Combined sources
Helixi59 – 7517Combined sources
Turni76 – 783Combined sources
Helixi81 – 833Combined sources
Beta strandi84 – 885Combined sources
Helixi90 – 945Combined sources
Helixi98 – 1058Combined sources
Turni106 – 1083Combined sources
Beta strandi113 – 1153Combined sources
Helixi118 – 13114Combined sources
Beta strandi139 – 1446Combined sources
Beta strandi149 – 1557Combined sources
Beta strandi158 – 1669Combined sources
Helixi169 – 1768Combined sources
Helixi178 – 1803Combined sources
Helixi184 – 19613Combined sources
Turni197 – 2015Combined sources
Helixi202 – 2087Combined sources
Beta strandi211 – 2177Combined sources
Helixi218 – 22811Combined sources
Turni229 – 2313Combined sources
Helixi239 – 25012Combined sources
Helixi255 – 2573Combined sources
Helixi265 – 2684Combined sources
Helixi271 – 28515Combined sources
Beta strandi290 – 2923Combined sources
Helixi297 – 30913Combined sources
Helixi314 – 32613Combined sources
Helixi330 – 35021Combined sources
Turni352 – 3543Combined sources
Helixi357 – 36913Combined sources
Helixi372 – 3754Combined sources
Helixi381 – 39111Combined sources
Helixi399 – 41012Combined sources
Beta strandi412 – 4143Combined sources
Helixi418 – 4203Combined sources
Beta strandi425 – 4273Combined sources
Helixi429 – 44416Combined sources
Turni445 – 4484Combined sources
Helixi449 – 4513Combined sources
Beta strandi459 – 4635Combined sources
Beta strandi466 – 4716Combined sources
Helixi475 – 4773Combined sources
Helixi480 – 49415Combined sources
Beta strandi500 – 5056Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FLOX-ray2.20A/B/C/D2-513[»]
ProteinModelPortaliP0AFL8.
SMRiP0AFL8. Positions 12-511.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFL8.

Family & Domainsi

Sequence similaritiesi

Belongs to the GppA/Ppx family.Curated

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiENNKLLH.
OrthoDBiEOG6M6JN6.

Family and domain databases

InterProiIPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03706. exo_poly_only. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFL8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG
60 70 80 90 100
LGPDNMLSEE AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD
110 120 130 140 150
FLKRAEKVIP YPIEIISGNE EARLIFMGVE HTQPEKGRKL VIDIGGGSTE
160 170 180 190 200
LVIGENFEPI LVESRRMGCV SFAQLYFPGG VINKENFQRA RMAAAQKLET
210 220 230 240 250
LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE RLEKLVKEVL
260 270 280 290 300
RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG
310 320 330 340 350
VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ
360 370 380 390 400
QPKLAHPQLE ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE
410 420 430 440 450
QQLMMATLVR YHRKAIKLDD LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ
460 470 480 490 500
ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA LVLLDLEKEQ EYWEGVAGWR
510
LKIEEESTPE IAA
Length:513
Mass (Da):58,136
Last modified:January 23, 2007 - v2
Checksum:i58E58E0F5D9FB9C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57612.1.
BA000007 Genomic DNA. Translation: BAB36787.1.
PIRiD91049.
H85893.
RefSeqiNP_289055.1. NC_002655.2.
NP_311391.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG57612; AAG57612; Z3765.
BAB36787; BAB36787; BAB36787.
GeneIDi914211.
957770.
KEGGiece:Z3765.
ecs:ECs3364.
PATRICi18356150. VBIEscCol44059_3270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57612.1 .
BA000007 Genomic DNA. Translation: BAB36787.1 .
PIRi D91049.
H85893.
RefSeqi NP_289055.1. NC_002655.2.
NP_311391.1. NC_002695.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FLO X-ray 2.20 A/B/C/D 2-513 [» ]
ProteinModelPortali P0AFL8.
SMRi P0AFL8. Positions 12-511.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z3765.

Proteomic databases

PRIDEi P0AFL8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG57612 ; AAG57612 ; Z3765 .
BAB36787 ; BAB36787 ; BAB36787 .
GeneIDi 914211.
957770.
KEGGi ece:Z3765.
ecs:ECs3364.
PATRICi 18356150. VBIEscCol44059_3270.

Phylogenomic databases

eggNOGi COG0248.
HOGENOMi HOG000258672.
KOi K01524.
OMAi ENNKLLH.
OrthoDBi EOG6M6JN6.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-3326-MONOMER.
ECOO157:PPX-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AFL8.

Family and domain databases

InterProi IPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
[Graphical view ]
Pfami PF02541. Ppx-GppA. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR03706. exo_poly_only. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiPPX_ECO57
AccessioniPrimary (citable) accession number: P0AFL8
Secondary accession number(s): P29014, P76981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3