P0AFL7 (PPX_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exopolyphosphatase
Short name=ExopolyPase
EC=3.6.1.11

Alternative name(s):
Metaphosphatase

Gene names

Name:	ppx
Ordered Locus Names:	c3020

Organism

Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

199310 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate By similarity.
Catalytic activity	(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the GppA/Ppx family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	polyphosphate catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	exopolyphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 513	512	Exopolyphosphatase		PRO_0000194301

Sequences

	Sequence		Length	Mass (Da)
	P0AFL7 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 58E58E0F5D9FB9C3	FASTA	513	58,136
	10 20 30 40 50 60 MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG LGPDNMLSEE 70 80 90 100 110 120 AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD FLKRAEKVIP YPIEIISGNE 130 140 150 160 170 180 EARLIFMGVE HTQPEKGRKL VIDIGGGSTE LVIGENFEPI LVESRRMGCV SFAQLYFPGG 190 200 210 220 230 240 VINKENFQRA RMAAAQKLET LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE 250 260 270 280 290 300 RLEKLVKEVL RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG 310 320 330 340 350 360 VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ QPKLAHPQLE 370 380 390 400 410 420 ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE QQLMMATLVR YHRKAIKLDD 430 440 450 460 470 480 LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA 490 500 510 LVLLDLEKEQ EYWEGVAGWR LKIEEESTPE IAA

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN81470.1.
RefSeq	NP_754902.1. NC_004431.1.
3D structure databases
ProteinModelPortal	P0AFL7.
SMR	P0AFL7. Positions 12-511.
ModBase	Search...
Protein-protein interaction databases
STRING	199310.c3020.
Proteomic databases
PRIDE	P0AFL7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN81470; AAN81470; c3020.
GeneID	1038687.
KEGG	ecc:c3020.
PATRIC	18283900. VBIEscCol75197_2850.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000258672.
KO	K01524.
OMA	IIGQRFE.
ProtClustDB	PRK10854.
Family and domain databases
InterPro	IPR022371. Exopolyphosphatase. IPR003695. Ppx_GppA. [Graphical view]
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
TIGRFAMs	TIGR03706. exo_poly_only. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPX_ECOL6

Accession

Primary (citable) accession number: P0AFL7
Secondary accession number(s): P29014, P76981

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program