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P0AFL7 (PPX_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exopolyphosphatase

Short name=ExopolyPase
EC=3.6.1.11
Alternative name(s):
Metaphosphatase
Gene names
Name:ppx
Ordered Locus Names:c3020
OrganismEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]
Taxonomic identifier199310 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate By similarity.

Catalytic activity

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

Cofactor

Magnesium By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the GppA/Ppx family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   LigandMagnesium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpolyphosphate catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionexopolyphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 513512Exopolyphosphatase
PRO_0000194301

Sequences

Sequence LengthMass (Da)Tools
P0AFL7 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 58E58E0F5D9FB9C3

FASTA51358,136
        10         20         30         40         50         60 
MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG LGPDNMLSEE 

        70         80         90        100        110        120 
AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD FLKRAEKVIP YPIEIISGNE 

       130        140        150        160        170        180 
EARLIFMGVE HTQPEKGRKL VIDIGGGSTE LVIGENFEPI LVESRRMGCV SFAQLYFPGG 

       190        200        210        220        230        240 
VINKENFQRA RMAAAQKLET LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE 

       250        260        270        280        290        300 
RLEKLVKEVL RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG 

       310        320        330        340        350        360 
VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ QPKLAHPQLE 

       370        380        390        400        410        420 
ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE QQLMMATLVR YHRKAIKLDD 

       430        440        450        460        470        480 
LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA 

       490        500        510 
LVLLDLEKEQ EYWEGVAGWR LKIEEESTPE IAA 

« Hide

References

[1]"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli."
Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R.
Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014075 Genomic DNA. Translation: AAN81470.1.
RefSeqNP_754902.1. NC_004431.1.

3D structure databases

ProteinModelPortalP0AFL7.
SMRP0AFL7. Positions 12-511.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING199310.c3020.

Proteomic databases

PRIDEP0AFL7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN81470; AAN81470; c3020.
GeneID1038687.
KEGGecc:c3020.
PATRIC18283900. VBIEscCol75197_2850.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000258672.
KOK01524.
OMAIIGQRFE.
OrthoDBEOG6M6JN6.
ProtClustDBPRK10854.

Enzyme and pathway databases

BioCycECOL199310:C3020-MONOMER.

Family and domain databases

InterProIPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
[Graphical view]
PfamPF02541. Ppx-GppA. 1 hit.
[Graphical view]
TIGRFAMsTIGR03706. exo_poly_only. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPX_ECOL6
AccessionPrimary (citable) accession number: P0AFL7
Secondary accession number(s): P29014, P76981
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: December 11, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families