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P0AFL6

- PPX_ECOLI

UniProt

P0AFL6 - PPX_ECOLI

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Protein

Exopolyphosphatase

Gene

ppx

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate.

Catalytic activityi

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

Cofactori

Magnesium.

GO - Molecular functioni

  1. exopolyphosphatase activity Source: EcoCyc
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. polyphosphate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciEcoCyc:PPX-MONOMER.
ECOL316407:JW2487-MONOMER.
MetaCyc:PPX-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolyphosphatase (EC:3.6.1.11)
Short name:
ExopolyPase
Alternative name(s):
Metaphosphatase
Gene namesi
Name:ppx
Ordered Locus Names:b2502, JW2487
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11403. ppx.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 513512ExopolyphosphatasePRO_0000194299Add
BLAST

Proteomic databases

PaxDbiP0AFL6.
PRIDEiP0AFL6.

Expressioni

Gene expression databases

GenevestigatoriP0AFL6.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-29140N.
IntActiP0AFL6. 9 interactions.
MINTiMINT-1285895.
STRINGi511145.b2502.

Structurei

Secondary structure

1
513
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 186
Beta strandi23 – 319
Beta strandi34 – 4310
Helixi48 – 503
Helixi59 – 7517
Turni76 – 783
Helixi81 – 833
Beta strandi84 – 885
Helixi90 – 945
Helixi98 – 1058
Turni106 – 1083
Beta strandi113 – 1153
Helixi118 – 13215
Beta strandi139 – 1446
Beta strandi149 – 1557
Beta strandi158 – 1669
Helixi169 – 1768
Helixi178 – 1803
Helixi184 – 19815
Turni199 – 2013
Helixi202 – 2087
Beta strandi211 – 2177
Helixi218 – 22912
Beta strandi233 – 2353
Helixi239 – 24911
Helixi255 – 2573
Helixi267 – 2693
Helixi271 – 28515
Beta strandi290 – 2923
Helixi297 – 30913
Helixi314 – 32512
Helixi330 – 35021
Helixi352 – 3543
Helixi357 – 36913
Turni370 – 3767
Helixi381 – 39111
Helixi399 – 41012
Beta strandi412 – 4143
Helixi429 – 44416
Turni445 – 4484
Helixi449 – 4513
Beta strandi459 – 4635
Beta strandi466 – 4716
Helixi475 – 4784
Helixi480 – 49314
Beta strandi500 – 5056

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U6ZX-ray1.90A/B1-513[»]
ProteinModelPortaliP0AFL6.
SMRiP0AFL6. Positions 12-511.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFL6.

Family & Domainsi

Sequence similaritiesi

Belongs to the GppA/Ppx family.Curated

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
InParanoidiP0AFL6.
KOiK01524.
OMAiENNKLLH.
OrthoDBiEOG6M6JN6.
PhylomeDBiP0AFL6.

Family and domain databases

InterProiIPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03706. exo_poly_only. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFL6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG
60 70 80 90 100
LGPDNMLSEE AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD
110 120 130 140 150
FLKRAEKVIP YPIEIISGNE EARLIFMGVE HTQPEKGRKL VIDIGGGSTE
160 170 180 190 200
LVIGENFEPI LVESRRMGCV SFAQLYFPGG VINKENFQRA RMAAAQKLET
210 220 230 240 250
LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE RLEKLVKEVL
260 270 280 290 300
RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG
310 320 330 340 350
VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ
360 370 380 390 400
QPKLAHPQLE ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE
410 420 430 440 450
QQLMMATLVR YHRKAIKLDD LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ
460 470 480 490 500
ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA LVLLDLEKEQ EYWEGVAGWR
510
LKIEEESTPE IAA
Length:513
Mass (Da):58,136
Last modified:January 23, 2007 - v2
Checksum:i58E58E0F5D9FB9C3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06129 Genomic DNA. Translation: AAA24415.1.
U00096 Genomic DNA. Translation: AAC75555.1.
AP009048 Genomic DNA. Translation: BAA16390.1.
PIRiA45333.
RefSeqiNP_416997.1. NC_000913.3.
YP_490730.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75555; AAC75555; b2502.
BAA16390; BAA16390; BAA16390.
GeneIDi12931600.
946970.
KEGGiecj:Y75_p2455.
eco:b2502.
PATRICi32120393. VBIEscCol129921_2599.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06129 Genomic DNA. Translation: AAA24415.1 .
U00096 Genomic DNA. Translation: AAC75555.1 .
AP009048 Genomic DNA. Translation: BAA16390.1 .
PIRi A45333.
RefSeqi NP_416997.1. NC_000913.3.
YP_490730.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1U6Z X-ray 1.90 A/B 1-513 [» ]
ProteinModelPortali P0AFL6.
SMRi P0AFL6. Positions 12-511.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29140N.
IntActi P0AFL6. 9 interactions.
MINTi MINT-1285895.
STRINGi 511145.b2502.

Proteomic databases

PaxDbi P0AFL6.
PRIDEi P0AFL6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75555 ; AAC75555 ; b2502 .
BAA16390 ; BAA16390 ; BAA16390 .
GeneIDi 12931600.
946970.
KEGGi ecj:Y75_p2455.
eco:b2502.
PATRICi 32120393. VBIEscCol129921_2599.

Organism-specific databases

EchoBASEi EB1375.
EcoGenei EG11403. ppx.

Phylogenomic databases

eggNOGi COG0248.
HOGENOMi HOG000258672.
InParanoidi P0AFL6.
KOi K01524.
OMAi ENNKLLH.
OrthoDBi EOG6M6JN6.
PhylomeDBi P0AFL6.

Enzyme and pathway databases

BioCyci EcoCyc:PPX-MONOMER.
ECOL316407:JW2487-MONOMER.
MetaCyc:PPX-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AFL6.
PROi P0AFL6.

Gene expression databases

Genevestigatori P0AFL6.

Family and domain databases

InterProi IPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
[Graphical view ]
Pfami PF02541. Ppx-GppA. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR03706. exo_poly_only. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon."
    Akiyama M., Crooke E., Kornberg A.
    J. Biol. Chem. 268:633-639(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp 70 superfamily."
    Reizer J., Reizer A., Saier M.H. Jr., Bork B., Sander C.
    Trends Biochem. Sci. 18:247-248(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO GPPA.

Entry informationi

Entry nameiPPX_ECOLI
AccessioniPrimary (citable) accession number: P0AFL6
Secondary accession number(s): P29014, P76981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3