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P0AFL6 (PPX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exopolyphosphatase
Short name=ExopolyPase
EC=3.6.1.11
Alternative name(s):
Metaphosphatase
Gene names
Name:ppx
Ordered Locus Names:b2502, JW2487
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate.

Catalytic activity

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

Cofactor

Magnesium.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the GppA/Ppx family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   LigandMagnesium
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processpolyphosphate catabolic process

Inferred from mutant phenotype PubMed 9143103. Source: EcoCyc

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionexopolyphosphatase activity

Inferred from direct assay Ref.1. Source: EcoCyc

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 513512Exopolyphosphatase
PRO_0000194299

Secondary structure

1 .................................................................................... 513
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AFL6 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 58E58E0F5D9FB9C3

FASTA51358,136
        10         20         30         40         50         60 
MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG LGPDNMLSEE 

        70         80         90        100        110        120 
AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD FLKRAEKVIP YPIEIISGNE 

       130        140        150        160        170        180 
EARLIFMGVE HTQPEKGRKL VIDIGGGSTE LVIGENFEPI LVESRRMGCV SFAQLYFPGG 

       190        200        210        220        230        240 
VINKENFQRA RMAAAQKLET LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE 

       250        260        270        280        290        300 
RLEKLVKEVL RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG 

       310        320        330        340        350        360 
VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ QPKLAHPQLE 

       370        380        390        400        410        420 
ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE QQLMMATLVR YHRKAIKLDD 

       430        440        450        460        470        480 
LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA 

       490        500        510 
LVLLDLEKEQ EYWEGVAGWR LKIEEESTPE IAA

« Hide

References

« Hide 'large scale' references
[1]"An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon."
Akiyama M., Crooke E., Kornberg A.
J. Biol. Chem. 268:633-639(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
Strain: K12.
[2]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp 70 superfamily."
Reizer J., Reizer A., Saier M.H. Jr., Bork B., Sander C.
Trends Biochem. Sci. 18:247-248(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO GPPA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L06129 Genomic DNA. Translation: AAA24415.1.
U00096 Genomic DNA. Translation: AAC75555.1.
AP009048 Genomic DNA. Translation: BAA16390.1.
PIRA45333.
RefSeqNP_416997.1. NC_000913.2.
YP_490730.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1U6ZX-ray1.90A/B1-513[»]
ProteinModelPortalP0AFL6.
SMRP0AFL6. Positions 12-511.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-29140N.
IntActP0AFL6. 7 interactions.
MINTMINT-1285895.
STRING511145.b2502.

Proteomic databases

PaxDbP0AFL6.
PRIDEP0AFL6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75555; AAC75555; b2502.
BAA16390; BAA16390; BAA16390.
GeneID12931600.
946970.
KEGGecj:Y75_p2455.
eco:b2502.
PATRIC32120393. VBIEscCol129921_2599.

Organism-specific databases

EchoBASEEB1375.
EcoGeneEG11403. ppx.

Phylogenomic databases

eggNOGCOG0248.
HOGENOMHOG000258672.
KOK01524.
OMAIIGQRFE.
ProtClustDBPRK10854.

Enzyme and pathway databases

BioCycEcoCyc:PPX-MONOMER.
ECOL316407:JW2487-MONOMER.
MetaCyc:PPX-MONOMER.

Gene expression databases

GenevestigatorP0AFL6.

Family and domain databases

InterProIPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
[Graphical view]
PfamPF02541. Ppx-GppA. 1 hit.
[Graphical view]
TIGRFAMsTIGR03706. exo_poly_only. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0AFL6.

Entry information

Entry namePPX_ECOLI
AccessionPrimary (citable) accession number: P0AFL6
Secondary accession number(s): P29014, P76981
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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