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Reviewed, UniProtKB/Swiss-Prot P0AFL6 (PPX_ECOLI)

Last modified June 16, 2009. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Exopolyphosphatase
      Short name=ExopolyPase
    EC=3.6.1.11
Alternative name(s):
    Metaphosphatase
Gene names
Name: ppx
Ordered Locus Names: b2502, JW2487
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate.

Catalytic activity

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

Cofactor

Magnesium.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the gppA/ppx family.

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Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   LigandMagnesium
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionexopolyphosphatase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 513512Exopolyphosphatase
PRO_0000194299

Secondary structure

1 .................................................................................... 513
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P0AFL6-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 58E58E0F5D9FB9C3

FASTA51358,136
        10         20         30         40         50         60 
MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG LGPDNMLSEE 

        70         80         90        100        110        120 
AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD FLKRAEKVIP YPIEIISGNE 

       130        140        150        160        170        180 
EARLIFMGVE HTQPEKGRKL VIDIGGGSTE LVIGENFEPI LVESRRMGCV SFAQLYFPGG 

       190        200        210        220        230        240 
VINKENFQRA RMAAAQKLET LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE 

       250        260        270        280        290        300 
RLEKLVKEVL RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG 

       310        320        330        340        350        360 
VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ QPKLAHPQLE 

       370        380        390        400        410        420 
ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE QQLMMATLVR YHRKAIKLDD 

       430        440        450        460        470        480 
LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA 

       490        500        510 
LVLLDLEKEQ EYWEGVAGWR LKIEEESTPE IAA

« Hide

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References

« Hide 'large scale' references
[1]"An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon."
Akiyama M., Crooke E., Kornberg A.
J. Biol. Chem. 268:633-639(1993) [PubMed: 8380170] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
Strain: K12.
[2]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp 70 superfamily."
Reizer J., Reizer A., Saier M.H. Jr., Bork B., Sander C.
Trends Biochem. Sci. 18:247-248(1993) [PubMed: 8212131] [Abstract]
Cited for: SIMILARITY TO GPPA.
+Additional computationally mapped references.

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Cross-references

Sequence databases

L06129 Genomic DNA. Translation: AAA24415.1.
U00096 Genomic DNA. Translation: AAC75555.1.
AP009048 Genomic DNA. Translation: BAA16390.1.
PIRA45333.
RefSeqAP_003088.1.
NP_416997.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1U6ZX-ray1.90A/B1-513[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:29140N.

Genome annotation databases

GeneID946970.
GenomeReviewsGene locus JW2487 in contig AP009048_GR.
Gene locus b2502 in contig U00096_GR.
KEGGecj:JW2487.
eco:b2502.

Organism-specific databases

EchoBASEEB1375.
EcoGeneEG11403. ppx.
CMRSearch...

Phylogenomic databases

HOGENOMP0AFL6.
OMAP0AFL6. VREAPNG.

Enzyme and pathway databases

BioCycEcoCyc:PPX-MON.
MetaCyc:PPX-MON.

Family and domain databases

InterProIPR003695. Ppx_GppA.
[Graphical view]
PfamPF02541. Ppx-GppA. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePPX_ECOLI
AccessionPrimary (citable) accession number: P0AFL6
Secondary accession number(s): P29014, P76981
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 33 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents