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Protein

Exopolyphosphatase

Gene

ppx

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate.

Catalytic activityi

(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

Cofactori

GO - Molecular functioni

  • exopolyphosphatase activity Source: EcoCyc

GO - Biological processi

  • polyphosphate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciEcoCyc:PPX-MONOMER.
ECOL316407:JW2487-MONOMER.
MetaCyc:PPX-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolyphosphatase (EC:3.6.1.11)
Short name:
ExopolyPase
Alternative name(s):
Metaphosphatase
Gene namesi
Name:ppx
Ordered Locus Names:b2502, JW2487
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11403. ppx.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001942992 – 513ExopolyphosphataseAdd BLAST512

Proteomic databases

PaxDbiP0AFL6.
PRIDEiP0AFL6.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4260594. 9 interactors.
DIPiDIP-29140N.
IntActiP0AFL6. 9 interactors.
MINTiMINT-1285895.
STRINGi511145.b2502.

Structurei

Secondary structure

1513
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 18Combined sources6
Beta strandi23 – 31Combined sources9
Beta strandi34 – 43Combined sources10
Helixi48 – 50Combined sources3
Helixi59 – 75Combined sources17
Turni76 – 78Combined sources3
Helixi81 – 83Combined sources3
Beta strandi84 – 88Combined sources5
Helixi90 – 94Combined sources5
Helixi98 – 105Combined sources8
Turni106 – 108Combined sources3
Beta strandi113 – 115Combined sources3
Helixi118 – 132Combined sources15
Beta strandi139 – 144Combined sources6
Beta strandi149 – 155Combined sources7
Beta strandi158 – 166Combined sources9
Helixi169 – 176Combined sources8
Helixi178 – 180Combined sources3
Helixi184 – 198Combined sources15
Turni199 – 201Combined sources3
Helixi202 – 208Combined sources7
Beta strandi211 – 217Combined sources7
Helixi218 – 229Combined sources12
Beta strandi233 – 235Combined sources3
Helixi239 – 249Combined sources11
Helixi255 – 257Combined sources3
Helixi267 – 269Combined sources3
Helixi271 – 285Combined sources15
Beta strandi290 – 292Combined sources3
Helixi297 – 309Combined sources13
Helixi314 – 325Combined sources12
Helixi330 – 350Combined sources21
Helixi352 – 354Combined sources3
Helixi357 – 369Combined sources13
Turni370 – 376Combined sources7
Helixi381 – 391Combined sources11
Helixi399 – 410Combined sources12
Beta strandi412 – 414Combined sources3
Helixi429 – 444Combined sources16
Turni445 – 448Combined sources4
Helixi449 – 451Combined sources3
Beta strandi459 – 463Combined sources5
Beta strandi466 – 471Combined sources6
Helixi475 – 478Combined sources4
Helixi480 – 493Combined sources14
Beta strandi500 – 505Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U6ZX-ray1.90A/B1-513[»]
ProteinModelPortaliP0AFL6.
SMRiP0AFL6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFL6.

Family & Domainsi

Sequence similaritiesi

Belongs to the GppA/Ppx family.Curated

Phylogenomic databases

eggNOGiENOG4105C9X. Bacteria.
COG0248. LUCA.
HOGENOMiHOG000258672.
InParanoidiP0AFL6.
KOiK01524.
OMAiFETKSRV.
PhylomeDBiP0AFL6.

Family and domain databases

InterProiIPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.
TIGRFAMsiTIGR03706. exo_poly_only. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG
60 70 80 90 100
LGPDNMLSEE AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD
110 120 130 140 150
FLKRAEKVIP YPIEIISGNE EARLIFMGVE HTQPEKGRKL VIDIGGGSTE
160 170 180 190 200
LVIGENFEPI LVESRRMGCV SFAQLYFPGG VINKENFQRA RMAAAQKLET
210 220 230 240 250
LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE RLEKLVKEVL
260 270 280 290 300
RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG
310 320 330 340 350
VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ
360 370 380 390 400
QPKLAHPQLE ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE
410 420 430 440 450
QQLMMATLVR YHRKAIKLDD LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ
460 470 480 490 500
ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA LVLLDLEKEQ EYWEGVAGWR
510
LKIEEESTPE IAA
Length:513
Mass (Da):58,136
Last modified:January 23, 2007 - v2
Checksum:i58E58E0F5D9FB9C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06129 Genomic DNA. Translation: AAA24415.1.
U00096 Genomic DNA. Translation: AAC75555.1.
AP009048 Genomic DNA. Translation: BAA16390.1.
PIRiA45333.
RefSeqiNP_416997.1. NC_000913.3.
WP_001121363.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75555; AAC75555; b2502.
BAA16390; BAA16390; BAA16390.
GeneIDi946970.
KEGGiecj:JW2487.
eco:b2502.
PATRICi32120393. VBIEscCol129921_2599.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06129 Genomic DNA. Translation: AAA24415.1.
U00096 Genomic DNA. Translation: AAC75555.1.
AP009048 Genomic DNA. Translation: BAA16390.1.
PIRiA45333.
RefSeqiNP_416997.1. NC_000913.3.
WP_001121363.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U6ZX-ray1.90A/B1-513[»]
ProteinModelPortaliP0AFL6.
SMRiP0AFL6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260594. 9 interactors.
DIPiDIP-29140N.
IntActiP0AFL6. 9 interactors.
MINTiMINT-1285895.
STRINGi511145.b2502.

Proteomic databases

PaxDbiP0AFL6.
PRIDEiP0AFL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75555; AAC75555; b2502.
BAA16390; BAA16390; BAA16390.
GeneIDi946970.
KEGGiecj:JW2487.
eco:b2502.
PATRICi32120393. VBIEscCol129921_2599.

Organism-specific databases

EchoBASEiEB1375.
EcoGeneiEG11403. ppx.

Phylogenomic databases

eggNOGiENOG4105C9X. Bacteria.
COG0248. LUCA.
HOGENOMiHOG000258672.
InParanoidiP0AFL6.
KOiK01524.
OMAiFETKSRV.
PhylomeDBiP0AFL6.

Enzyme and pathway databases

BioCyciEcoCyc:PPX-MONOMER.
ECOL316407:JW2487-MONOMER.
MetaCyc:PPX-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFL6.
PROiP0AFL6.

Family and domain databases

InterProiIPR022371. Exopolyphosphatase.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.
TIGRFAMsiTIGR03706. exo_poly_only. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPX_ECOLI
AccessioniPrimary (citable) accession number: P0AFL6
Secondary accession number(s): P29014, P76981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.