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P0AFL6

- PPX_ECOLI

UniProt

P0AFL6 - PPX_ECOLI

Protein

Exopolyphosphatase

Gene

ppx

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate.

    Catalytic activityi

    (Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. exopolyphosphatase activity Source: EcoCyc
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. polyphosphate catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciEcoCyc:PPX-MONOMER.
    ECOL316407:JW2487-MONOMER.
    MetaCyc:PPX-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exopolyphosphatase (EC:3.6.1.11)
    Short name:
    ExopolyPase
    Alternative name(s):
    Metaphosphatase
    Gene namesi
    Name:ppx
    Ordered Locus Names:b2502, JW2487
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11403. ppx.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 513512ExopolyphosphatasePRO_0000194299Add
    BLAST

    Proteomic databases

    PaxDbiP0AFL6.
    PRIDEiP0AFL6.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AFL6.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    DIPiDIP-29140N.
    IntActiP0AFL6. 9 interactions.
    MINTiMINT-1285895.
    STRINGi511145.b2502.

    Structurei

    Secondary structure

    1
    513
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi13 – 186
    Beta strandi23 – 319
    Beta strandi34 – 4310
    Helixi48 – 503
    Helixi59 – 7517
    Turni76 – 783
    Helixi81 – 833
    Beta strandi84 – 885
    Helixi90 – 945
    Helixi98 – 1058
    Turni106 – 1083
    Beta strandi113 – 1153
    Helixi118 – 13215
    Beta strandi139 – 1446
    Beta strandi149 – 1557
    Beta strandi158 – 1669
    Helixi169 – 1768
    Helixi178 – 1803
    Helixi184 – 19815
    Turni199 – 2013
    Helixi202 – 2087
    Beta strandi211 – 2177
    Helixi218 – 22912
    Beta strandi233 – 2353
    Helixi239 – 24911
    Helixi255 – 2573
    Helixi267 – 2693
    Helixi271 – 28515
    Beta strandi290 – 2923
    Helixi297 – 30913
    Helixi314 – 32512
    Helixi330 – 35021
    Helixi352 – 3543
    Helixi357 – 36913
    Turni370 – 3767
    Helixi381 – 39111
    Helixi399 – 41012
    Beta strandi412 – 4143
    Helixi429 – 44416
    Turni445 – 4484
    Helixi449 – 4513
    Beta strandi459 – 4635
    Beta strandi466 – 4716
    Helixi475 – 4784
    Helixi480 – 49314
    Beta strandi500 – 5056

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1U6ZX-ray1.90A/B1-513[»]
    ProteinModelPortaliP0AFL6.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AFL6.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GppA/Ppx family.Curated

    Phylogenomic databases

    eggNOGiCOG0248.
    HOGENOMiHOG000258672.
    KOiK01524.
    OMAiENNKLLH.
    OrthoDBiEOG6M6JN6.
    PhylomeDBiP0AFL6.

    Family and domain databases

    InterProiIPR022371. Exopolyphosphatase.
    IPR003695. Ppx_GppA.
    [Graphical view]
    PfamiPF02541. Ppx-GppA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03706. exo_poly_only. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AFL6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG    50
    LGPDNMLSEE AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD 100
    FLKRAEKVIP YPIEIISGNE EARLIFMGVE HTQPEKGRKL VIDIGGGSTE 150
    LVIGENFEPI LVESRRMGCV SFAQLYFPGG VINKENFQRA RMAAAQKLET 200
    LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE RLEKLVKEVL 250
    RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG 300
    VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ 350
    QPKLAHPQLE ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE 400
    QQLMMATLVR YHRKAIKLDD LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ 450
    ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA LVLLDLEKEQ EYWEGVAGWR 500
    LKIEEESTPE IAA 513
    Length:513
    Mass (Da):58,136
    Last modified:January 23, 2007 - v2
    Checksum:i58E58E0F5D9FB9C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06129 Genomic DNA. Translation: AAA24415.1.
    U00096 Genomic DNA. Translation: AAC75555.1.
    AP009048 Genomic DNA. Translation: BAA16390.1.
    PIRiA45333.
    RefSeqiNP_416997.1. NC_000913.3.
    YP_490730.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75555; AAC75555; b2502.
    BAA16390; BAA16390; BAA16390.
    GeneIDi12931600.
    946970.
    KEGGiecj:Y75_p2455.
    eco:b2502.
    PATRICi32120393. VBIEscCol129921_2599.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06129 Genomic DNA. Translation: AAA24415.1 .
    U00096 Genomic DNA. Translation: AAC75555.1 .
    AP009048 Genomic DNA. Translation: BAA16390.1 .
    PIRi A45333.
    RefSeqi NP_416997.1. NC_000913.3.
    YP_490730.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1U6Z X-ray 1.90 A/B 1-513 [» ]
    ProteinModelPortali P0AFL6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29140N.
    IntActi P0AFL6. 9 interactions.
    MINTi MINT-1285895.
    STRINGi 511145.b2502.

    Proteomic databases

    PaxDbi P0AFL6.
    PRIDEi P0AFL6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75555 ; AAC75555 ; b2502 .
    BAA16390 ; BAA16390 ; BAA16390 .
    GeneIDi 12931600.
    946970.
    KEGGi ecj:Y75_p2455.
    eco:b2502.
    PATRICi 32120393. VBIEscCol129921_2599.

    Organism-specific databases

    EchoBASEi EB1375.
    EcoGenei EG11403. ppx.

    Phylogenomic databases

    eggNOGi COG0248.
    HOGENOMi HOG000258672.
    KOi K01524.
    OMAi ENNKLLH.
    OrthoDBi EOG6M6JN6.
    PhylomeDBi P0AFL6.

    Enzyme and pathway databases

    BioCyci EcoCyc:PPX-MONOMER.
    ECOL316407:JW2487-MONOMER.
    MetaCyc:PPX-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AFL6.
    PROi P0AFL6.

    Gene expression databases

    Genevestigatori P0AFL6.

    Family and domain databases

    InterProi IPR022371. Exopolyphosphatase.
    IPR003695. Ppx_GppA.
    [Graphical view ]
    Pfami PF02541. Ppx-GppA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR03706. exo_poly_only. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon."
      Akiyama M., Crooke E., Kornberg A.
      J. Biol. Chem. 268:633-639(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp 70 superfamily."
      Reizer J., Reizer A., Saier M.H. Jr., Bork B., Sander C.
      Trends Biochem. Sci. 18:247-248(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY TO GPPA.

    Entry informationi

    Entry nameiPPX_ECOLI
    AccessioniPrimary (citable) accession number: P0AFL6
    Secondary accession number(s): P29014, P76981
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 72 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3