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Protein

Peptidyl-prolyl cis-trans isomerase A

Gene

ppiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.1 Publication

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

Enzyme regulationi

Inhibition by cyclosporin A with a Ki of 25 to 50 mu-mol, a concentration 100-fold higher than that required for eukaryotic PPIases.

GO - Molecular functioni

  • peptidyl-prolyl cis-trans isomerase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Rotamase

Enzyme and pathway databases

BioCyciEcoCyc:EG10757-MONOMER.
ECOL316407:JW3326-MONOMER.
MetaCyc:EG10757-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase A (EC:5.2.1.8)
Short name:
PPIase A
Alternative name(s):
Cyclophilin A
Rotamase A
Gene namesi
Name:ppiA
Synonyms:rot, rotA
Ordered Locus Names:b3363, JW3326
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10757. ppiA.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi136F → W: Enhances susceptibility to CSA inhibition. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000002549725 – 190Peptidyl-prolyl cis-trans isomerase AAdd BLAST166

Proteomic databases

EPDiP0AFL3.
PaxDbiP0AFL3.
PRIDEiP0AFL3.

2D gel databases

SWISS-2DPAGEP0AFL3.

Interactioni

Protein-protein interaction databases

BioGridi4261085. 126 interactors.
DIPiDIP-48080N.
STRINGi511145.b3363.

Structurei

Secondary structure

1190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 35Combined sources6
Beta strandi38 – 44Combined sources7
Turni46 – 48Combined sources3
Helixi50 – 61Combined sources12
Turni62 – 67Combined sources6
Beta strandi69 – 74Combined sources6
Turni75 – 77Combined sources3
Beta strandi78 – 84Combined sources7
Helixi101 – 103Combined sources3
Beta strandi111 – 114Combined sources4
Beta strandi118 – 121Combined sources4
Beta strandi127 – 132Combined sources6
Helixi135 – 137Combined sources3
Beta strandi148 – 154Combined sources7
Helixi156 – 163Combined sources8
Beta strandi167 – 170Combined sources4
Beta strandi173 – 179Combined sources7
Beta strandi182 – 188Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CLHNMR-A25-190[»]
1J2AX-ray1.80A25-190[»]
1V9TX-ray1.70A/B25-190[»]
1VAIX-ray1.80A/B25-190[»]
ProteinModelPortaliP0AFL3.
SMRiP0AFL3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFL3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 188PPIase cyclophilin-typePROSITE-ProRule annotationAdd BLAST162

Sequence similaritiesi

Belongs to the cyclophilin-type PPIase family.Curated
Contains 1 PPIase cyclophilin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108R5K. Bacteria.
COG0652. LUCA.
HOGENOMiHOG000065978.
InParanoidiP0AFL3.
KOiK03767.
OMAiRDMKEKP.
PhylomeDBiP0AFL3.

Family and domain databases

Gene3Di2.40.100.10. 1 hit.
InterProiIPR029000. Cyclophilin-like_dom.
IPR024936. Cyclophilin-type_PPIase.
IPR020892. Cyclophilin-type_PPIase_CS.
IPR002130. Cyclophilin-type_PPIase_dom.
[Graphical view]
PANTHERiPTHR11071. PTHR11071. 1 hit.
PfamiPF00160. Pro_isomerase. 1 hit.
[Graphical view]
PRINTSiPR00153. CSAPPISMRASE.
SUPFAMiSSF50891. SSF50891. 1 hit.
PROSITEiPS00170. CSA_PPIASE_1. 1 hit.
PS50072. CSA_PPIASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKSTLAAMA AVFALSALSP AAMAAKGDPH VLLTTSAGNI ELELDKQKAP
60 70 80 90 100
VSVQNFVDYV NSGFYNNTTF HRVIPGFMIQ GGGFTEQMQQ KKPNPPIKNE
110 120 130 140 150
ADNGLRNTRG TIAMARTADK DSATSQFFIN VADNAFLDHG QRDFGYAVFG
160 170 180 190
KVVKGMDVAD KISQVPTHDV GPYQNVPSKP VVILSAKVLP
Length:190
Mass (Da):20,431
Last modified:December 20, 2005 - v1
Checksum:i8B48535F36AA61A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55429 Genomic DNA. Translation: AAA23451.1.
M28363 Genomic DNA. Translation: AAA23772.1.
U18997 Genomic DNA. Translation: AAA58160.1.
U00096 Genomic DNA. Translation: AAC76388.1.
AP009048 Genomic DNA. Translation: BAE77927.1.
M32354 Genomic DNA. Translation: AAA24261.1.
PIRiA37964. CSECA.
RefSeqiNP_417822.1. NC_000913.3.
WP_000477225.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76388; AAC76388; b3363.
BAE77927; BAE77927; BAE77927.
GeneIDi947870.
KEGGiecj:JW3326.
eco:b3363.
PATRICi32122160. VBIEscCol129921_3457.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55429 Genomic DNA. Translation: AAA23451.1.
M28363 Genomic DNA. Translation: AAA23772.1.
U18997 Genomic DNA. Translation: AAA58160.1.
U00096 Genomic DNA. Translation: AAC76388.1.
AP009048 Genomic DNA. Translation: BAE77927.1.
M32354 Genomic DNA. Translation: AAA24261.1.
PIRiA37964. CSECA.
RefSeqiNP_417822.1. NC_000913.3.
WP_000477225.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CLHNMR-A25-190[»]
1J2AX-ray1.80A25-190[»]
1V9TX-ray1.70A/B25-190[»]
1VAIX-ray1.80A/B25-190[»]
ProteinModelPortaliP0AFL3.
SMRiP0AFL3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261085. 126 interactors.
DIPiDIP-48080N.
STRINGi511145.b3363.

2D gel databases

SWISS-2DPAGEP0AFL3.

Proteomic databases

EPDiP0AFL3.
PaxDbiP0AFL3.
PRIDEiP0AFL3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76388; AAC76388; b3363.
BAE77927; BAE77927; BAE77927.
GeneIDi947870.
KEGGiecj:JW3326.
eco:b3363.
PATRICi32122160. VBIEscCol129921_3457.

Organism-specific databases

EchoBASEiEB0750.
EcoGeneiEG10757. ppiA.

Phylogenomic databases

eggNOGiENOG4108R5K. Bacteria.
COG0652. LUCA.
HOGENOMiHOG000065978.
InParanoidiP0AFL3.
KOiK03767.
OMAiRDMKEKP.
PhylomeDBiP0AFL3.

Enzyme and pathway databases

BioCyciEcoCyc:EG10757-MONOMER.
ECOL316407:JW3326-MONOMER.
MetaCyc:EG10757-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AFL3.
PROiP0AFL3.

Family and domain databases

Gene3Di2.40.100.10. 1 hit.
InterProiIPR029000. Cyclophilin-like_dom.
IPR024936. Cyclophilin-type_PPIase.
IPR020892. Cyclophilin-type_PPIase_CS.
IPR002130. Cyclophilin-type_PPIase_dom.
[Graphical view]
PANTHERiPTHR11071. PTHR11071. 1 hit.
PfamiPF00160. Pro_isomerase. 1 hit.
[Graphical view]
PRINTSiPR00153. CSAPPISMRASE.
SUPFAMiSSF50891. SSF50891. 1 hit.
PROSITEiPS00170. CSA_PPIASE_1. 1 hit.
PS50072. CSA_PPIASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPIA_ECOLI
AccessioniPrimary (citable) accession number: P0AFL3
Secondary accession number(s): P20752, Q2M729
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.