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Protein

Putrescine transport system permease protein PotI

Gene

potI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the activity of the bacterial periplasmic transport system of putrescine.

GO - Molecular functioni

  • putrescine-importing ATPase activity Source: EcoCyc

GO - Biological processi

  • putrescine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:POTI-MONOMER.
ECOL316407:JW0841-MONOMER.
MetaCyc:POTI-MONOMER.
RETL1328306-WGS:GSTH-5503-MONOMER.
RETL1328306-WGS:GSTH-732-MONOMER.

Protein family/group databases

TCDBi3.A.1.11.2. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine transport system permease protein PotI
Gene namesi
Name:potI
Ordered Locus Names:b0857, JW0841
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11632. potI.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3320HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini34 – 6835PeriplasmicSequence analysisAdd
BLAST
Transmembranei69 – 8820HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini89 – 11527CytoplasmicSequence analysisAdd
BLAST
Transmembranei116 – 13520HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini136 – 1405PeriplasmicSequence analysis
Transmembranei141 – 16020HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini161 – 18626CytoplasmicSequence analysisAdd
BLAST
Transmembranei187 – 20620HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini207 – 24337PeriplasmicSequence analysisAdd
BLAST
Transmembranei244 – 26320HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini264 – 28118CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281Putrescine transport system permease protein PotIPRO_0000060189Add
BLAST

Proteomic databases

PaxDbiP0AFL1.
PRIDEiP0AFL1.

Interactioni

Protein-protein interaction databases

BioGridi4259996. 3 interactions.
STRINGi511145.b0857.

Structurei

3D structure databases

ProteinModelPortaliP0AFL1.
SMRiP0AFL1. Positions 8-238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 260196ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D38. Bacteria.
COG1177. LUCA.
HOGENOMiHOG000263700.
InParanoidiP0AFL1.
KOiK11074.
OMAiKPWKVFF.
OrthoDBiEOG66XBH9.
PhylomeDBiP0AFL1.

Family and domain databases

Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AFL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNLPVVRSP WRIVILLLGF TFLYAPMLML VIYSFNSSKL VTVWAGWSTR
60 70 80 90 100
WYGELLRDDA MMSAVGLSLT IAACAATAAA ILGTIAAVVL VRFGRFRGSN
110 120 130 140 150
GFAFMITAPL VMPDVITGLS LLLLFVALAH AIGWPADRGM LTIWLAHVTF
160 170 180 190 200
CTAYVAVVIS SRLRELDRSI EEAAMDLGAT PLKVFFVITL PMIMPAIISG
210 220 230 240 250
WLLAFTLSLD DLVIASFVSG PGATTLPMLV FSSVRMGVNP EINALATLIL
260 270 280
GAVGIVGFIA WYLMARAEKQ RIRDIQRARR G
Length:281
Mass (Da):30,541
Last modified:December 20, 2005 - v1
Checksum:iA1B557CE2FC7C9EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93239 Genomic DNA. Translation: AAA24412.1.
U00096 Genomic DNA. Translation: AAC73944.1.
AP009048 Genomic DNA. Translation: BAA35568.1.
PIRiD45313.
RefSeqiNP_415378.1. NC_000913.3.
WP_001061658.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73944; AAC73944; b0857.
BAA35568; BAA35568; BAA35568.
GeneIDi945485.
KEGGiecj:JW0841.
eco:b0857.
PATRICi32116919. VBIEscCol129921_0885.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93239 Genomic DNA. Translation: AAA24412.1.
U00096 Genomic DNA. Translation: AAC73944.1.
AP009048 Genomic DNA. Translation: BAA35568.1.
PIRiD45313.
RefSeqiNP_415378.1. NC_000913.3.
WP_001061658.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AFL1.
SMRiP0AFL1. Positions 8-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259996. 3 interactions.
STRINGi511145.b0857.

Protein family/group databases

TCDBi3.A.1.11.2. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP0AFL1.
PRIDEiP0AFL1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73944; AAC73944; b0857.
BAA35568; BAA35568; BAA35568.
GeneIDi945485.
KEGGiecj:JW0841.
eco:b0857.
PATRICi32116919. VBIEscCol129921_0885.

Organism-specific databases

EchoBASEiEB1589.
EcoGeneiEG11632. potI.

Phylogenomic databases

eggNOGiENOG4105D38. Bacteria.
COG1177. LUCA.
HOGENOMiHOG000263700.
InParanoidiP0AFL1.
KOiK11074.
OMAiKPWKVFF.
OrthoDBiEOG66XBH9.
PhylomeDBiP0AFL1.

Enzyme and pathway databases

BioCyciEcoCyc:POTI-MONOMER.
ECOL316407:JW0841-MONOMER.
MetaCyc:POTI-MONOMER.
RETL1328306-WGS:GSTH-5503-MONOMER.
RETL1328306-WGS:GSTH-732-MONOMER.

Miscellaneous databases

PROiP0AFL1.

Family and domain databases

Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome."
    Pistocchi R., Kashiwagi K., Miyamoto S., Nukui E., Sadakata Y., Kobayashi H., Igarashi K.
    J. Biol. Chem. 268:146-152(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPOTI_ECOLI
AccessioniPrimary (citable) accession number: P0AFL1
Secondary accession number(s): P31136
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 20, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.